Background: Worldwide, children represent a higher proportion of the population, with children younger than age 15 accounting for 1.8 billion (28%) of the world’s 6.4 billion persons. Venipuncture and other invasive procedures (blood draws, intramuscular injections, heel pricks) are the most commonly performed painful procedures in children during hospitalization. Venipuncture is a stressful and painful experience for children. Nurses are in a unique position to improve the management of children’s pain; because they are often the professionals who have the most contact with an ill child. Objectives: The main objective of the study was to evaluate the effectiveness of distraction technique on pain and cooperation level during venipuncture among school aged children. Materials and Methods: True experimental – Post-test only design was employed. The study was conducted at pediatrics ward, Institute of Child Health and Research Centre, Government Rajaji Hospital, Madurai. A total of 60 subjects were selected using simple random sampling technique (30 in experimental group and 30 in control group). Children in the experimental group were displayed the cartoon movie on the laptop during the whole duration of the venipuncture. The usual standard technique was given for the children in control group. Numerical Pain Rating Scale was used for measurement of pain level and Cooperative Behavior Scale of Children in Venipuncture (CBSCV) was used to assess the cooperation level. Findings: In the experimental group majority of the children 43% had no pain and 70% had good cooperation. Whereas, in control group children 53% had severe pain and 70% had no cooperation. The obtained ‘t’ value for pain level 12.07 was significant at p<0.001 level. Similarly the obtained ‘t’ value for cooperation level 11.67 was significant at p<0.001 level. The correlation between the pain level and cooperation level, in experimental group was ‘r’= 0.62 significant at p<0.001 level and in control group was ‘r’= 0.57 significant at p<0.001 level. So, there was highly positive correlation between the pain level and the cooperation level during venipuncture. The number of previous venipuncture was significantly associated with the pain and cooperation level of the children in the experimental and control group. Conclusion: The study concluded that distraction technique was effective in reducing the pain and promoting the cooperation of school aged children during venipuncture. This distracting technique is highly recommended because it is effective, easy to carry out and inexpensive.