Fine Sugar Research Articles

EFFECT OF SUGAR MIXTURES WITH DIFFERENT TYPES ON RHEOLOGICAL, PHYSICOCHEMICAL AND SENSORIAL QUALITY OF BISCUITS

Sugar mixtures with different types are commonly used in biscuit formulation. This study investigates the mixture of crystal sugar (CS) and fine sugar (FS) for the quality of biscuits in terms of rheological, sensorial and physical properties. Mixture design approach was also used and the effect of model was significant for all dependent variables such as Tan ? in rheological, hardness and spread ratio (SR) in physical, general acceptability in sensorial analysis. With the crystal sugar decrement in the formulation, a reduction was observed for Tan ? values. High level replacement of CS with FS (from 50% to 100%) causes dramatic increase in SR values of biscuits. Decreasing the replacement level of CS increased the hardness value of biscuits. The results of this study showed that the type of sugar and the mixing ratio can significantly affect the physicochemical and sensory quality of the biscuit. The biscuit sample containing the 90% FS and 10% CS (F6) obtained the highest score for hardness, crispness, and general acceptability.

EFFECTS OF KOH ON SOME BIOCHAR COMPONENTS FROM PYROLYZED FINE SUGAR BAGASSE (FSB)

In this paper the effect of mixing KOH on biochar components from pyrolyzed fine sugar bagasse (FSB) was studied. The fine sugar bagasse (FSB) was ground and mixed in batches with KOH at different weight ratios, such as 0, 20 and 40 %, to form 10-mm pellets, which were then thermal analyzed to identyfy the sutiable pyrolyze temperature. The FSB-derived biochars were characterized using DSC/TG, XRD, SEM/EDX to determine the effect of KOH content on the FSB-derived biochar. Experimen data shown that the obtain biochar posses different pore size vary from 0.5 ~ 1.0 µm. In case of using KOH, the silica from fine sugar bagasse can reacted with KOH, CO2 to form K2SiO3 and K2CO3 on the surface of biochar. Different amount of C can be capture by biochar by vary amount of KOH used. The treatment of biomass product can be used for rice hush, rice hush ash, and also sugarcane bagasse. These biochars can be used as a potential source for biochar-based power plant materials, or to remove toxic substances from wastewater. Based on the results of our study, we can recommend an activation of FSB-derived biochar for further use.

Impact of junk food on health

Junk food refers to fast food, easy to make and easy to consume. Their low nutritional value and just lay down fat in it cause adverse effects on Health of consumer. The term 'Junk food' was created by Michael Jacobson, director of the Center for Science in 1972 in common interests who want to improve public concern about problem foods with high calorific value and low a nutritional value. Junk food contains high levels of fine sugar, white flour, trans fats, polyunsaturated fats and fat salt various food additives such as monosodium glutamate (MSG) and tartrazine, and the lack of protein, vitamins and fiber. The junk food advertising is also play a great role in junk food’s popularity. But it should be avoided, because of lack of energy, high cholesterol and poor concentration. It causes a lot of harmful effect on the body like obesity, diabetes, heart disease and various types of skin cancers. In this review article we will see what are different types of junk food, how it attracts most of people and how to reduce the consumption of junk food. Keywords: Junk food, Cholesterol, Fat, Salt, Sugar, Obesity, Concentration, Advertisement.

Rice Bran Ice Cream Characteristics on Various Proportions of Rice Bran Flour with Skimmed Milk and Kinds of Stabilizer

This research aimed to study rice bran ice cream on treatments of proportions of rice bran flour with skim milk ( 0:100; 25:75; and 50 : 50) and three kinds of stabilizers i.e. CMC; Carageenan; and CMC : carageenan 1 :1. Ice cream was made by mixing skim milk with rice bran flour in accordance with treatments, whipped cream and water then pas- teurized at temperature 75 oC until boiled (dough 1). Dough 2. was made of fine sugar, egg yolk and stabilizers in accordance with treatments then mixed by mixer. Dough 1 and 2 then mixed in heating process at temperature 75 oC then conditioned in ambi- ent temperature. Conditioned dough then stored an hour in refrigerator and stirred by mixer in a minute before it stored in freezer for three hours as further cooling. After the first cooling, ice cream was stirred again by mixer until soft texture was obtained. Those process then repeated once an hour until ice cream desired texture was obtained. Che- mical and physical characteristics of ice cream were observed with three replications. Data obtained were descripted and analized by regression. Result of the research showed that the higher proportions of rice bran flour with skim milk the higher fat content, crude fiber, and melting rate of rice bran ice cream. Carageenan gave highest overrun, while combination of carageenan and CMC resulted in highest melting rate and viscosity of rice bran ice cream.

Non-structural carbohydrate concentrations of Fagus sylvatica and Pinus sylvestris fine roots are linked to ectomycorrhizal enzymatic activity during spring reactivation

We evaluated whether changes in fine root non-structural carbohydrate reserves of Fagus sylvatica and Pinus sylvestris trees influence potential enzymatic activities of their ectomycorrhizal symbionts from winter towards spring reactivation, and whether these changes influence potential soil enzymatic activities. We analyzed sugar and starch concentrations in the fine roots of Fagus sylvatica and Pinus sylvestris and potential activities of ß-glucosidase, ß-xylosidase, and cellobiohydrolase (as proxies for carbon-degrading enzymes) as well as leucine aminopeptidase and chitinase (as proxies for nitrogen-degrading enzymes) of their dominant ectomycorrhizal symbionts as well as in the soil. Sugar concentrations in the fine roots were significantly positively correlated with enzymatic activities of the ectomycorrhizal symbionts. In Pinus sylvestris, both carbon- and nitrogen-degrading enzyme activities showed significant positive correlations with fine root sugar concentrations. In Fagus sylvatica, fine root sugar concentrations were explicitly positively correlated with the activity of nitrogen-degrading enzymes. The chitinase activity in the soil was found to be strongly positively correlated with the enzymatic activity of the ectomycorrhizal symbionts as well as with fine root sugar concentrations. Fine root carbohydrate concentrations of Fagus sylvatica and Pinus sylvestris trees and enzymatic activities of their associated ectomycorrhizal fungi are connected. The specific nutrient demand of the tree species during spring reactivation may affect ectomycorrhizal enzymatic activity via carbon mobilization in the fine roots of Fagus sylvatica and Pinus sylvestris. Moreover, our results suggest that trees indirectly contribute to the degradation of fungal necromass by stimulating ectomycorrhizal chitinase activity in the soil.

Purification, characterization and fine sugar specificity of a N-Acetylgalactosamine specific lectin from Adenia hondala.

Plant lectins are gaining interest because of their interesting biological properties. Several Adenia species, that are being used in traditional medicine to treat many health ailments have shown presence of lectins or carbohydrate binding proteins. Here, we report the purification, characterization and biological significance of N-Acetyl galactosamine specific lectin from Adenia hondala (AHL) from Passifloraceae family. AHL was purified in a single step by affinity chromatography on asialofetuin Sepharose 4B column, characterized and its fine sugar specificity determined by glycan array analysis. AHL is human blood group non specific and also agglutinates rabbit erythrocytes. AHL is a glycoprotein with 12.5% of the carbohydrate, SDS-PAGE, MALDI-TOF-MS and ESI-MS analysis showed that AHL is a monomer of 31.6kDa. AHL is devoid of DNase activity unlike other Ribosome inactivating proteins (RIPs). Glycan array analysis of AHL revealed its highest affinity for terminal lactosamine or polylactosamine of N- glycans, known to be over expressed in hepatocellular carcinoma and colon cancer. AHL showed strong binding to human hepatocellular carcinoma HepG2 cells with MFI of 59.1 expressing these glycans which was effectively blocked by 93.1% by asialofetuin. AHL showed dose and time dependent growth inhibitory effects on HepG2 cells with IC50 of 4.8μg/ml. AHL can be explored for its clinical potential.

The electrical resistivity and relative permittivity of binary powder mixtures

Effective electrical resistivities and static relative permittivities of binary powder mixtures were measured and compared to theoretical equations given by Generalized Mixture Rule (GMR). The measured values for mixtures were influenced by the resistivity and permittivity of the pure component materials, the particle sizes and by the sticking between the different particles. According to the Random Model (RM), the effective resistivity and relative permittivity of any randomly packed mixture material can be calculated from the component resistivities and relative permittivities, respectively, and their concentrations. It was shown that RM gave good predictions for both quantities for powders if the particle sizes of the component materials were almost the same. However, this was not the case when large NaCl particles were mixed with fine sugar particles. When these mixtures were prepared, finer, more numerous sugar particles coated the larger NaCl particles, and the mixtures were not random but closer to the series configuration. Effect of particle size was studied briefly by mixing very fine NaCl particles with sugar of similar size. With these mixtures, results predicted by RM were obtained.

Sauromatum guttatum lectin: Spectral studies, lectin-carbohydrate interaction, molecular cloning and in silico analysis

Non-immune carbohydrate binding proteins are broadly defined as lectins. Having been reported from all kingdoms of life, phytolectins are the most widely studied group of lectins. Sauromatum guttatum agglutinin (SGA) was isolated from the plant tubers and characterized for structural variations due to solvent perturbation using polarimetry, fluorescence and light scattering. For the β-sheet rich SGA, a pH and temperature induced molten globule like intermediate was identified. In isothermal titration microcalorimetry, SGA demonstrated cooperative binding to a complex glycoprotein in enthalpically driven mechanism. Fine sugar specificity exploration identified core pentasaccharide as the most common and highest binding motif with complex N-glycans and fucosylated core N-glycans as additional motifs. Molecular cloning of SGA which has previously been demonstrated to have anti-cancer and anti-insect activities is being reported for the first time. Full length cDNA sequence was obtained with RACE-PCR based upon the conserved carbohydrate recognition site [QXDXNXVXY] present in all GNA-related lectins. Quaternary structure was proposed by homology modeling and an attempt was made to explain the structure-function relationship by in silico analysis.

Purification, characterization and biological significance of mannose binding lectin from Dioscorea bulbifera bulbils

Dioscorea bulbifera or air potato has been used as a folk remedy to treat cancer. A mannose binding lectin from bulbils of D. bulbifera was purified in a single step by affinity chromatography on mucin coupled Sepharose 4B column, determined by its fine sugar specificity by glycan array analysis and studied for its clinical potential in cancer and HIV research.SDS-PAGE showed that lectin is a monomer of Mr 24kDa. DBL agglutinated only rabbit erythrocytes and was inhibited by mucin, asialomucin, fetuin, asialofetuin and transferrin but not by any monosaccharides. Glycan array analysis of DBL revealed its affinity toward high mannose N-linked glycans with enhanced affinity for terminal mannose including N-linked glycans of HIV envelope glycoprotein gp120 and has strong anti-reverse transcriptase activity. DBL showed strong binding to non-metastatic human colon epithelial cancer HT 29, metastatic SW 620 and hepatocellular HepG2 cell lines. DBL showed dose and time dependent growth inhibitory effects on all the three cell lines HT 29, SW 620 and HepG2 with IC50 of 110μg, 9.8μg, 40μg respectively at 72h. Inhibitory effect of DBL was effectively blocked in presence of competing glycans like mucin. DBL has promising clinical potential both in cancer and HIV research.

Exquisite specificity of mitogenic lectin from Cephalosporium curvulum to core fucosylated N-glycans.

Lectins are carbohydrate binding proteins that are gaining attention as important tools for the identification of specific glycan markers expressed during different stages of the cancer. We earlier reported the purification of a mitogenic lectin from human pathogenic fungus Cephalosporium curvulum (CSL) that has complex sugar specificity when analysed by hapten inhibition assay. In the present study, we report the fine sugar specificity of CSL as determined by glycan array analysis. The results revealed that CSL has exquisite specificity towards core fucosylated N-glycans. Fucosylated trimannosyl core is the basic structure required for the binding of CSL. The presence of fucose in the side chain further enhances the avidity of CSL towards such glycans. The affinity of CSL is drastically reduced towards the non-core fucosylated glycans, in spite of their side chain fucosylation. CSL showed no binding to the tested O-glycans and monosaccharides. These observations suggest the unique specificity of CSL towards core fucosylated N-glycans, which was further validated by binding of CSL to human colon cancer epithelial and hepatocarcinoma cell lines namely HT29 and HepG2, respectively, that are known to express core fucosylated N-glycans, using AOL and LCA as positive controls. LCA and AOL are fucose specific lectins that are currently being used clinically for the diagnosis of hepatocellular carcinomas. Most of the gastrointestinal markers express core fucosylated N-glycans. The high affinity and exclusive specificity of CSL towards α1-6 linkage of core fucosylated glycans compared to other fucose specific lectins, makes it a promising molecule that needs to be further explored for its application in the diagnosis of gastrointestinal cancer.

Interaction of chondroitin sulfate and dermatan sulfate from various biological sources with heparin-binding growth factors and cytokines

Chondroitin sulfate (CS) and dermatan sulfate (DS) interact with various extracellular molecules such as growth factors, cytokines/chemokines, neurotrophic factors, morphogens, and viral proteins, thereby playing roles in a variety of biological processes including cell adhesion, proliferation, tissue morphogenesis, neurite outgrowth, infections, and inflammation/leukocyte trafficking. CS/DS are modified with sulfate groups at C-2 of uronic acid residues as well as C-4 and/or C-6 of N-acetyl-D-galactosamine residues, yielding enormous structural diversity, which enables the binding with numerous proteins. We have demonstrated that highly sulfated CS-E from squid cartilage, for example, interacts with heparin-binding proteins including midkine, pleiotrophin, and fibroblast growth factors expressed in brain with high affinity (Kd values in the nM range). Here, we analyzed the binding of CS and DS, which have a relatively low degree of sulfation and have been widely used as a nutraceutical and a drug for osteoarthritis etc., with a number of heparin-binding neurotrophic factors/cytokines using surface plasmon resonance (SPR) and structurally characterized the CS/DS chains. SPR showed that relatively low sulfated CS-A, DS, and CS-C also bound with significant affinity to midkine, pleiotrophin, hepatocyte growth factor, monokine-induced by interferon-γ, and stromal cell derived factor-1β, although the binding was less intense than that with highly sulfated CS-D and CS-E. These findings suggest that even low sulfated CS and/or DS chains may contain binding domains, which include fine sugar sequences with specific sulfation patterns, and that sugar sequences, conformations and electrostatic potential are more important than the simple degree of sulfation represented by disaccharide composition.

Influence of conching temperature and some bulk sweeteners on physical and rheological properties of prebiotic milk chocolate containing inulin

Changes in food consumption habits and the developments set forth in the area of health and nutrition also change consumer expectations and demands. Sugar-free foodstuffs and products that have prebiotic activity are among the primary features of such expectations and demands. In the present study, the effects of substituting fine sugar with isomalt and maltitol in milk chocolate samples that contain inulin (9.0 % w/w), which is a substance with prebiotic activity, and the use of varying conching temperatures (CT) (50, 55 and 60 °C) in the sample preparation process on their physical (colour, hardness, water activity) and rheological properties were examined. Rheological data were obtained using the Herschel–Bulkley model which showed the best fitting for predicting rheology. It was determined that all properties included within the scope of the study are affected by the use of different bulk sweeteners or varying CT (P < 0.01). While colour properties, such as brightness (L*), hue angle (h°), water activity (aw) and rate index properties varied in a narrow range, it was determined that the yield stress and viscosity properties, which are among the important quality parameters of chocolate and can have determining effects on sensory properties, manifest variations within a broad range, depending on the CT and the bulk sweeteners used. It was concluded that maltitol is a more suitable fine sugar substitute in milk chocolates containing inulin.

Characterization of a New α-Galactosyl-Binding Lectin from the MushroomClavaria purpurea

A galactose specific lectin (CpL) was purified from the Clavaria purpurea mushroom by affinity chromatography. CpL agglutinated only trypsin-treated rabbit erythrocytes. On enzyme linked lectin sorbent assay (ELLSA), the lectin bound with thyroglobulin and asialo bovine submaxillary mucin (BSM). The fine sugar binding specificity of CpL was elucidated using inhibition of hemagglutination and sugar immobilized gold nano-particles (SGNP). The results indicated a preference of CpL towards α-galactosyl sugar chains. Among several monosaccharides and disaccharides assayed for dissociation effect on the SGNP-CpL complex, Galα1-3Gal and raffinose were the best inhibitors. The partial amino acid sequence showed two QXW motifs in CpL and similarity towards members of the ricin B superfamily.

Binding of Escherichia coli to Functionalized Gold Nanoparticles

The molecular basis of the diversity of fimbrial lectins dictates the extent of adhesion in different types of Escherichia coli strains to mammalian cells. The mechanism of receptor binding by E. coli in eukaryotic cells differs based on the adhesin domains, patterns in the macromolecular structure and the ligand-binding groove. Current sensor technologies utilize biosensors that are based on the carbohydrate moieties that are involved in pathogen adhesion to host cells. Nanoparticles have been extensively used as carriers for pathogen detection. Gold nanoparticles (Au NPs) of 200 nm size were functionalized with two distinct glycoconjugates mannose (Mn–Au NPs) and Neuαc(α2-3)-Gal-(β1-4)Glc–Paa (Sg–Au NPs) in order to investigate primary and fine sugar specificity of uropathogenic E. coli ORN178 and enterotoxigenic E. coli 13762, respectively. The UV-Vis measurement of pristine, 16-mercaptohexadecanoic acid (MHDA)/2-(2-aminoethoxy)ethanol (AEE)/sugar functionalized Au NPs showed a surface plasmon resonance band for Au. Dynamic light scattering analysis showed that the mean averages of the MHDA/AEE/Mn–Au NP samples increased due to aggregation. The negative zeta potentials of the samples were indicative of aggregation. Fine sugar specificity was observed when Neuαc(α2-3)-Gal-(β1-4)Glc–Paa functionalized Au NPs (Sg–Au NPs) specifically showed binding with E. coli 13762 but not with E. coli ORN178. This specificity of E. coli strains to identify and bind to characteristic sugar moieties can be used in the development of biodiagnostic tools with Au NPs as carriers for diagnosis/treatment of human and veterinary diseases. In regards to the growing antibiotic resistance of microorganisms, gold nanoparticles can also be functionalized specifically to reverse adhesion of E. coli to host tissue and can be detected by their optical properties.

A reproducible test to characterise the triboelectric charging of powders during their pneumatic transport

Although several investigations had been carried out to explore the triboelectrification of powders, only few data are available on the experimental procedures and set-ups required to obtain reliable data. The present study deals with the development of a pneumatic test to characterise the tribocharging of fine powders. An experimental device was set up allowing on-line monitoring of the charge of both particles and transport pipes. Experiments were carried out using two types of powder (fine sugar and PVC) coupled with two types of pipe materials (Teflon and nylon). Results showed the extreme importance of the control of the relative humidity, the initial charge of particles and the charge dissipation of the walls to obtain pertinent data. Furthermore, the results showed that solids loadings higher than 1 (kg of solids/kg of air) are not proper to achieve reliable measurements. However, at very dilute solids loading (∼0.001) the time evolutions of the electrostatic charge and the mass of the powder follow similar trends so that the tribocharging becomes independent of the solids mass flowrate. This allows accurate assessment of the tribocharging of cohesive powders for which a regular flow cannot be guaranteed.

HCA and HML isolated from the red marine algae Hypnea cervicornis and Hypnea musciformis define a novel lectin family

HCA and HML represent lectins isolated from the red marine algae Hypnea cervicornis and Hypnea musciformis, respectively. Hemagglutination inhibition assays suggest that HML binds GalNAc/Gal substituted with a neutral sugar through 1-3, 1-4, or 1-2 linkages in O-linked mucin-type glycans, and Fuc(alpha1-6)GlcNAc of N-linked glycoproteins. The specificity of HCA includes the epitopes recognized by HML, although the glycoproteins inhibited distinctly HML and HCA. The agglutinating activity of HCA was inhibited by GalNAc, highlighting the different fine sugar epitope-recognizing specificity of each algal lectin. The primary structures of HCA (9193+/-3 Da) and HML (9357+/-1 Da) were determined by Edman degradation and tandem mass spectrometry of the N-terminally blocked fragments. Both lectins consist of a mixture of a 90-residue polypeptide containing seven intrachain disulfide bonds and two disulfide-bonded subunits generated by cleavage at the bond T50-E51 (HCA) and R50-E51 (HML). The amino acid sequences of HCA and HML display 55% sequence identity (80% similarity) between themselves, but do not show discernible sequence and cysteine spacing pattern similarities with any other known protein structure, indicating that HCA and HML belong to a novel lectin family. Alignment of the amino acid sequence of the two lectins revealed the existence of internal domain duplication, with residues 1-47 and 48-90 corresponding to the N- and C-terminal domains, respectively. The six conserved cysteines in each domain may form three intrachain cysteine linkages, and the unique cysteine residues of the N-terminal (Cys46) and the C-terminal (Cys71) domains may form an intersubunit disulfide bond.

Interactions in dispersions of sugar particles in food oils: influence of emulsifier

The influence of oil-soluble surface-active components on the interactions of fine sugar particles dispersed in triglyceride oils has been investigated at different volume fractions by a combination of rheological and sedimentation experiments. Four different types of food oil were investigated: soybean, milk fat, lauric fat (palm kernel oil) and cocoa butter. The addition of a low concentration of emulsifier, soya lecithin (phosphatidylcholine) or monoolein, was used to adjust the nature of the interactions between the hydrophilic sugar particles. In a dispersion of 70 wt% sugar and 0.3% of lecithin, around 30–40% of the emulsifier present was found to be adsorbed at the surface of the sugar particles. The viscosities of the dispersions in the different oils were found to be similar up to 30 wt% sugar content. At high sugar contents (30–60 wt%), however, differences between the oil samples were evident. Lauric fat generated the highest viscosity amongst the different oils whereas cocoa butter exhibited the lowest. The presence of the emulsifier lowered the viscosity of the dispersions to differing extents. Gravity settling observations were made for dispersions of 10 wt% sugar particles in the different oils in the presence and absence of emulsifier. The emulsifiers were effective in reducing the volume of sediment and hence in increasing the sediment particle packing density. This implies that the adsorbed surface-active species reduce the strength of the attractive interactions between the sedimenting sugar particles, in good agreement with the rheological data at high sugar contents.

The use of different sugars as fine and coarse carriers for aerosolised salbutamol sulphate

The aim of this study was to investigate the dispersion and deaggregation of a model drug, salbutamol sulphate (SS), using lactose, mannitol or sorbitol as coarse and fine carriers. Binary and tertiary formulations containing micronised salbutamol sulphate (SS) and sieved (63–90 μm) coarse sugar crystals or salbutamol sulphate (SS) with a mixture of coarse and fine sugar particles were prepared. Factorial design was employed to investigate the effects of three variables, i.e. the chemical entity of the coarse sugar carrier, the chemical entity of the fine sugar and the concentration of fine sugar, on the dispersion and deaggregation of salbutamol sulphate after aerosolisation at 60 l/min via a Rotahaler® into a twin stage liquid impinger (TSI). The binary formulations containing the different sugar entities produced differences in the fine (<6.4 μm) particle fraction (FPF) of SS in a decreasing order of mannitol>sorbitol>lactose, but failed to produce efficient dispersion of SS since the FPF was <10%. Adding fine sugar particles and increasing their concentration to the binary mixtures generally resulted in an increase in the FPF of salbutamol sulphate. The chemical nature of the fine carriers was found to play a less important role in determining respirable fraction of the drug than the coarse carriers. In conclusion, other sugars such as mannitol or sorbitol, besides lactose, may be employed as coarse and/or fine carriers for incorporation into dry powder aerosol formulations to increase FPF.

Further characterization of the glycan-binding specificity of the seed lectin from vatairea macrocarpa and its dependence of pH

Abstract: The lectin from Vatairea macrocarpa seeds exhibits a pronounced specificity for glycoproteins possessing Gal/GalNAc residues in their carbohydrate moieties. Determination of the fine sugar specificity of the lectin by affinity chromatography of labelled oligosaccharides and glycopeptides of known structures on the immobilized lectin shows that the lectin precisely recognizes non reducing N acetyllactosamine sequence. This interaction seems to depend of the number of N-acetyllactosamine sequence in each structure and is dependent of pH.

Fine sugar specificity of the mistletoe (Viscum album) lectin I

The behaviour of N-acetyllactosamine-type oligosaccharides and glycopeptides on a column of mistletoe lectin I (MLI) immobilized on Sepharose 4B was examined. The immobilized lectin does not show any affinity for asialo-N-glycosylpeptides and related oligosaccharides, which possess one to four unmasked N-acetyllactosamine sequences. However, substitution of at least one of the N-acetyllactosamine sequences by sialic acid residues, either at O-3 or O-6 of galactose, slightly enhances the affinity of the lectin. Such sialylated N-glycosylpeptides or oligosaccharides are eluted from the lectin column by the starting buffer as retarded fractions. Surprisingly, the affinity of the immobilized MLI is higher for P1 antigen-containing glycopeptide isolated from turtle-dove ovomucoid and for glycopeptides from bovine thyroglobulin containing terminal non-reducing Gal alpha 1-3Gal sequences. These structures are strongly bound on the lectin column and their elution is obtained with 0.15 M galactose in the starting buffer.