Final Segments Research Articles

Development of morphological technique for segmentation of anatomical objects in abdominal MRI

In this paper, a morphological technique for the segmentation of abdominal organs in magnetic resonance imaging (MRI) images is proposed based on watershed segmentation. New morphological based preprocessing and post-processing techniques are developed to reduce oversegmentation by means of removing and merging spurious segments. The preprocessing aims at removing trivial regions as well as background noise by combining thresholding, morphological smoothing, Gaussian smoothing and morphological edge detection. To obtain a more concise region representation, the watershed segmented image is post-processed, where a region adjacency list is built for the region merging process that produces the final segments. To control the merging process, a similarity function is defined, whence the most similar neighbouring regions are merged. The proposed technique produces effective and significant results in successfully segmenting various anatomical objects in axial MRI images of the abdomen, as it is shown in this paper.

Enhanced phonological facilitation and traces of concurrent word form activation in speech production: An object-naming study with multiple distractors

In the present study, we extended the classic picture-word interference paradigm by the presentation of multiple distractor words (Experiment 1) to reexamine whether the word forms of semantic alternatives receive activation in the course of object naming. Experiment 2 showed that phonological facilitation can be magnified by the presentation of multiple words that share overlapping initial and final segments with the target name. Experiments 3 and 4 tested for traces ofnontarget phonological activation with multiple distractors, which enhances the chances of detecting such effects. These experiments revealed a consistent pattern of interference effects induced by words that were related to a semantic category member, consistent with theories assuming phonological activation of nontarget alternatives.

Experimental validation of metabolic pathway modeling

In the search for new drug targets in the human parasite Entamoeba histolytica, metabolic control analysis was applied to determine, experimentally, flux control distribution of amebal glycolysis. The first (hexokinase, hexose-6-phosphate isomerase, pyrophosphate-dependent phosphofructokinase (PP(i)-PFK), aldolase and triose-phosphate isomerase) and final (3-phosphoglycerate mutase, enolase and pyruvate phosphate dikinase) glycolytic segments were reconstituted in vitro with recombinant enzymes under near-physiological conditions of pH, temperature and enzyme proportion. Flux control was determined by titrating flux with each enzyme component. In parallel, both glycolytic segments were also modeled by using the rate equations and kinetic parameters previously determined. Because the flux control distribution predicted by modeling and that determined by reconstitution were not similar, kinetic interactions among all the reconstituted components were experimentally revised to unravel the causes of the discrepancy. For the final segment, it was found that 3-phosphoglycerate was a weakly competitive inhibitor of enolase, whereas PP(i) was a moderate inhibitor of 3-phosphoglycerate mutase and enolase. For the first segment, PP(i) was both a strong inhibitor of aldolase and a nonessential mixed-type activator of amebal hexokinase; in addition, lower V(max) values for hexose-6-phosphate isomerase, PP(i)-PFK and aldolase were induced by PP(i) or ATP inhibition. It should be noted that PP(i) and other metabolites were absent from the 3-phosphoglycerate mutase and enolase or aldolase and hexokinase kinetics experiments, but present in reconstitution experiments. Only by incorporating these modifications in the rate equations, modeling predicted values of flux control distribution, flux rate and metabolite concentrations similar to those experimentally determined. The experimentally validated segment models allowed 'in silico experimentation' to be carried out, which is not easy to achieve in in vivo or in vitro systems. The results predicted a nonsignificant effect on flux rate and flux control distribution by adding parallel routes (pyruvate kinase for the final segment and ATP-dependent PFK for the first segment), because of the much lower activity of these enzymes in the ameba. Furthermore, modeling predicted full flux-control by 3-phosphoglycerate mutase and hexokinase, in the presence of low physiological substrate and product concentrations. It is concluded that the combination of in vitro pathway reconstitution with modeling and enzyme kinetics experimentation permits a more comprehensive understanding of the pathway behavior and control properties.

Quantitative analysis of individual metal-CdSe-metal nanowire field-effect transistors

Heterostructured metal-CdSe-metal nanowires were fabricated by sequential electrochemical deposition of layers of Au and the semiconductor CdSe. Nonlinear I-V curves were observed, and a parameter retrieval model was used to extract the majority carrier mobility of 0.5cm2V−1s−1 for nanowires fabricated with zero deposition current during the exchange of the final CdSe and Au segments. This improved threefold with the application of a small current during the solution exchange. Values for the resistance and the electron density for these nanowires were determined.

A case of myiasis in a patient with psoriasis from Turkey

Myiasis infestations caused by the larvae of flies mostly belonging to Cyclorapha suborders are frequently encountered in Turkey, which is located in the subtropical zone. The skin is a common site for myiasis, and the infestations are likely to develop in infected tissues and poorly attended wounds of the skin.The case, a 30-year-old male patient, was diagnosed with psoriasis 18 years ago. He had psoriatic scales on his right big toe and was receiving corticosteroid and immunosuppressive drugs.A total of 11 fly larvae were removed from the infected right first toe of the patient. Structures of the stigmas seen in the cross-sections taken from the final segments of these larvae were examined and determined as Sarcophaga spp. larvae.Patients with infected tissues should be extremely cautious about their wound hygiene and take required fly control measures especially during summer as flies can find a suitable environment for sustenance.

Incidence structures and Stone–Priestley duality

We observe that if R: = (I,ρ, J) is an incidence structure, viewed as a matrix, then the topological closure of the set of columns is the Stone space of the Boolean algebra generated by the rows. As a consequence, we obtain that the topological closure of the collection of principal initial segments of a poset P is the Stone space of the Boolean algebra Tailalg (P) generated by the collection of principal final segments of P, the so-called tail-algebra of P. Similar results concerning Priestley spaces and distributive lattices are given. A generalization to incidence structures valued by abstract algebras is considered.

A local-coloring method for night-vision colorization utilizing image analysis and fusion

A natural color mapping method has been previously proposed that matches the statistical properties (mean and standard deviation) of night-vision (NV) imagery to those of a daylight color image (manually selected as the “target” color distribution). Thus the rendered NV image appears to resemble the natural target image in terms of color appearance. However, in this prior method (termed “global-coloring”) the colored NV image may appear unnatural if the target image’s “global” color statistics are different from that of the night-vision scene (e.g., it would appear to have too much green if much more vegetation was contained in the target image). Consequently, a new “local-coloring” method is presented that functions to render the NV image segment-by-segment by taking advantage of image segmentation, pattern recognition, histogram matching and image fusion. Specifically, a false-color image (source image) is formed by assigning multi-band NV images to three RGB (red, green and blue) channels. A nonlinear diffusion filter is then applied to the false-colored image to reduce the number of colors. The final grayscale segments are obtained by using clustering and merging techniques. With a supervised nearest-neighbor paradigm, a segment can be automatically associated with a known “color scheme”. The statistic-matching procedure is merged with the histogram-matching procedure to enhance the color mapping effect. Instead of extracting the color set from a single target image, the mean, standard deviation and histogram distribution of the color planes from a set of natural scene images are used as the target color properties for each color scheme. The target color schemes are grouped by their scene contents and colors such as plants, mountain, roads, sky, water, etc. In our experiments, five pairs of night-vision images were initially analyzed, and the images that were colored (segment-by-segment) by the proposed “local-coloring” method are shown to possess much more natural and realistic coloration when compared with those produced by the previous “global-coloring” method.

The effect of alcohol ingestion on ocular counterrolling

Nineteen normal subjects underwent two naso-occipital rotations to 90 degrees right and left ear down in our standard ocular counterrolling (OCR) protocol. Both eyes were videotaped. Following two rotations, subjects drank 90 ml vodka in 180 ml orange juice; and in about 20 min, when blood alcohol levels reached 0.04-0.09%, the protocol was repeated. An SMI videooculography system provided measurements. Results showed that amplitude of OCR was significantly decreased after alcohol ingestion; smoothness was significantly increased after alcohol, similar to alcohol's effect on essential tremor. Although disconjugacy was not significantly different in the two conditions when the entire trials were examined, the latter portions of the post-alcohol trials did show significant disconjugacy, similar to earlier findings in vestibular-defective patients whose OCR deficits were apparent only in the final segments of the rotation trials. We postulate the results are due to alcohol's action on cerebellar GABAergic Purkinje cells projecting to vestibular nuclei.

A Syntactic and Prosodic Analysis of Utterance Final Segments : in Explanation-Giving Dialogues among Young Japanese

A Syntactic and Prosodic Analysis of Utterance Final Segments : in Explanation-Giving Dialogues among Young Japanese

Time pressure and phonological advance planning in spoken production

Current accounts of spoken production debate the extent to which speakers plan ahead. Here, we investigated whether the scope of phonological planning is influenced by changes in time pressure constraints. The first experiment used a picture–word interference task and showed that picture naming latencies were shorter when word distractors shared the final segments with the picture name. Experiment 2 used the same paradigm but with colored pictures to elicit determiner + adjective + noun phrases. Latencies were shorter when the distractor overlapped phonologically with the picture name. Finally, Experiment 3 demonstrated that in colored picture naming without distractors, latencies were shorter when the object noun began with the same phoneme as the color adjective. Crucially, in all experiments introduction of a response deadline accelerated latencies, but did not alter the relative magnitude of the priming effects. In sum, pressure to provide a swift response does not reduce the scope of phonological planning.

Linear and nonlinear fetal heart rate analysis of normal and acidemic fetuses in the minutes preceding delivery

Linear and nonlinear fetal heart rate (FHR) indices, namely mean FHR, interval index (II), very low, low and high frequencies, approximate (ApEn) and sample entropy (SampEn), were computed, immediately before delivery, in the initial and final FHR tracing segments, from 48 normal, 10 mildly acidemic and 10 moderate-to-severely acidemic fetuses. Progression of labor was associated with a significant increase in linear frequency domain indices whereas nonlinear indices were significantly decreased. Moderate-to-severe fetal acidemia was associated with a significant decrease in nonlinear indices. The best discrimination between moderate-to-severe acidemic fetuses and the remaining cases was obtained combining II and ApEn(2,0.15), with a specificity of 71% and a sensitivity of 80%. These findings support the hypothesis of increased autonomic nervous system activity in the final minutes of labor and of decreased central nervous system activity, both in the final minutes of labor and in moderate-to-severe acidemic fetuses.

Neocortical Network ActivityIn VivoIs Generated through a Dynamic Balance of Excitation and Inhibition

The recurrent excitatory and inhibitory connections between and within layers of the cerebral cortex are fundamental to the operation of local cortical circuits. Models of cortical function often assume that recurrent excitation and inhibition are balanced, and we recently demonstrated that spontaneous network activity in vitro contains a precise balance of excitation and inhibition; however, the existence of a balance between excitation and inhibition in the intact and spontaneously active cerebral cortex has not been directly tested. We examined this hypothesis in the prefrontal cortex in vivo, during the slow (<1 Hz) oscillation in ketamine-xylazine-anesthetized ferrets. We measured persistent network activity (Up states) with extracellular multiple unit and local field potential recording, while simultaneously recording synaptic currents in nearby cells. We determined the reversal potential and conductance change over time during Up states and found that the body of Up state activity exhibited a steady reversal potential (-37 mV on average) for hundreds of milliseconds, even during substantial (21 nS on average) changes in membrane conductance. Furthermore, we found that both the initial and final segments of the Up state were characterized by significantly more depolarized reversal potentials and concomitant increases in excitatory conductance, compared with the stable middle portions of Up states. This ongoing temporal evolution between excitation and inhibition, which exhibits remarkable proportionality within and across neurons in active local networks, may allow for rapid transitions between relatively stable network states, permitting the modulation of neuronal responsiveness in a behaviorally relevant manner.

Effects of auditory pattern structure on anticipatory and reactive attending

In three experiments, participants listened for a target’s pitch change within recurrent nine-tone patterns having largely isochronous rhythms. Patterns differed in pitch structure of initial (context) and final (target distance) pattern segments. Also varied were: probe timing (Experiments 2 and 3) and instructions about probe timing (Experiments 2 and 3). In all experiments, identification of a recurrent target was poorer in patterns with wider context pitch intervals (in semitones) than in others. Effects of probe timing also occurred, with better performance for temporally expected than unexpected probes. However, when listeners were explicitly told to focus upon a target’s pitch and not its timing (Experiment 3), they performed selectively better in patterns with smaller target/probe pitch distances, especially for rhythmically expected probes. Five theoretical approaches to the respective roles of pitch and/or time structure were assessed. Although no single approach accounted for all results, a modification of one theory (a Pitch/Time Entrainment model) provided a reasonable description of findings.

Internal versus external intrapartum foetal heart rate monitoring: the effect on linear and nonlinear parameters

The effect of foetal heart rate (FHR) acquisition mode on linear and nonlinear parameters is still largely unknown. In 33 normal labouring women, FHR signals were acquired simultaneously by an external ultrasound sensor applied to the maternal abdomen and an internal scalp electrode, in the minutes preceding delivery. For each case, the initial and final 5, 10 and 20 min segments were analysed, considering FHR signals at a frequency of 4 Hz (the frequency at which they are transmitted by the majority of commercialized foetal monitors). Several time and frequency domain linear and nonlinear FHR indices were computed in these segments, namely mean FHR, very low frequency (VLF), low frequency (LF), high frequency (HF), approximate entropy (ApEn) and sample entropy (SampEn). Parametric confidence intervals, statistical tests and correlation coefficients were calculated in order to evaluate the effect of internal versus external FHR monitoring modes on the considered indices. The whole evaluation was repeated using FHR signals at a frequency of 2 Hz. Most time domain linear indices were similar with external and internal monitoring in the initial and final segments of the tracings. However, linear frequency domain indices were poorly correlated in the final segments and had significantly different mean values in the initial segments. Nonlinear indices were significantly different in both initial and final segments. The correlation between 4 and 2 Hz sampled parameters was high for both linear and nonlinear indices (most correlation coefficient values ranging between 0.95 and 1) but nonlinear index values were significantly higher at 2 Hz. In conclusion, the mode used to acquire FHR signals and the sampling rate employed can significantly affect most FHR indices.

Patterns of English phoneme confusions by native and non-native listeners

Native American English and non-native (Dutch) listeners identified either the consonant or the vowel in all possible American English CV and VC syllables. The syllables were embedded in multispeaker babble at three signal-to-noise ratios (0, 8, and 16 dB). The phoneme identification performance of the non-native listeners was less accurate than that of the native listeners. All listeners were adversely affected by noise. With these isolated syllables, initial segments were harder to identify than final segments. Crucially, the effects of language background and noise did not interact; the performance asymmetry between the native and non-native groups was not significantly different across signal-to-noise ratios. It is concluded that the frequently reported disproportionate difficulty of non-native listening under disadvantageous conditions is not due to a disproportionate increase in phoneme misidentifications.

A graph‐searching method for MLC leaf sequencing under constraints

A new leaf-sequencing algorithm for step-and-shoot IMRT that is based on a graph-searching technique is described. An iterative process guided by a quantitative measure for the complexity of the initial or residual intensity pattern is used to identify the field segments shaped by a multileaf collimator (MLC). Given a user selected number of intensity levels, the algorithm searches deliverable segment candidates considering all intensity levels and two collimator positions separated by 90 degrees. The candidates for each intensity level are obtained as the least number of segments to cover the areas with equal or higher intensity. The shape of a deliverable segment is adjusted by leaving out certain beam elements for later delivery if this results in a simpler residual intensity pattern and the segment is still deliverable. For a MLC design that does not allow leaf interdigitation, it is initially assumed that a single segment cannot cover two disjoined areas. Among all candidates the segment with the greatest reduction of the complexity of the residual intensity distribution is chosen for the current step of iteration. The iterative process generates a set of deliverable segments of simply connected areas. These segments are combined later under specific MLC constraints. Different orders of segment combination are considered for minimizing the beam-on time. The final segments are sequenced to minimize the leaf travel. This algorithm has been tested using randomly generated intensity distributions and clinical cases for the Varian, Siemens, and Elekta MLC systems. The results show that as the number of intensity levels is increased, the numbers of segments and MUs increase only modestly. Using two collimator angles results in decreases in the required number of segments and the number of monitor units that can be as much as 20%.

Segment sequencing and the jaw cycle

The open–close jaw cycle established in running speech often spans the articulation of several segments. Moreover, the extremes of the cycle are associated with opposing segment types, consonants and vowels, which form higher-level linguistic units, syllables, when grouped. The present study sought to determine how the syllable is related to the cycle. A partial relationship may imply that the jaw moves linearly from one segment to another. An isomorphic relationship that jaw movement is planned over the articulation of a group of segments, regardless of segment sequencing within the group. Five speakers repeated one of five syllable types (CV, VC, CVC, CCV, VCC) continuously for several seconds, producing an unbroken sequence of consonants and vowels. Jaw movement and acoustic data were recorded simultaneously and used to determine the temporal boundaries of the cycles and syllables, respectively. Cycle boundaries were defined by the points of maximum closure preceding and following an opening gesture, and syllable boundaries according to changes in frequency and amplitude corresponding to the onset of the initial and final segments in the syllable. A comparison of the relative boundary locations suggested an isomorphic relationship between cycles and syllables that interacted with segment type, syllable shape, and speaker.

The onset effect in word naming

This study investigates whether or not masked form priming effects in the naming task depend on the number of shared segments between prime and target. Dutch participants named bisyllabic words, which were preceded by visual masked primes. When primes shared the initial segment(s) with the target, naming latencies were shorter than in a control condition (string of percent signs). Onset complexity (singleton vs. complex word onset) did not modulate this priming effect in Dutch. Furthermore, significant priming due to shared final segments was only found when the prime did not contain a mismatching onset, suggesting an interfering role of initial non-target segments. It is concluded that (a) degree of overlap (segmental match vs. mismatch), and (b) position of overlap (initial vs. final) influence the magnitude of the form priming effect in the naming task. A modification of the segmental overlap hypothesis ( Schiller, 1998) is proposed to account for the data.

Regulated-stereoselective construction of thirteen stereogenic centers necessary for the frame of (+)-discodermolide, based on iterative Lewis acid-promoted aldol reactions.

The segments C(1)-C(13) and C(15)-C(21) containing the 13 stereogenic centers required for the frame of (+)-discodermolide were synthesized in good to excellent enantio- and diastereoselectivities from a common racemic aldehyde, derived from 2-methyl-1,3-propanediol. The enantioselective aldol reactions of the racemic aldehyde with a silylketene acetal, derived from ethyl 2-bromopropionate, in the presence of chiral oxazaborolidinones, prepared in situ with N-p-toluenesulfonyl-(R)- and -(S)-valine and BH(3).THF, proceeded under kinetic control to give the stereotriads with a high degree of enantioselectivity. Enantioselective (chiral borane) and diastereoselective (BF(3).OEt(2) and TiCl(4)) aldol reactions with the silylketene acetal, coupled with diastereoselective radical debrominations (Bu(3)SnH, Et(3)B, with or without MgBr(2)), were used iteratively. This aldol reaction strategy for the construction of the polypropionate frame dramatically shortened the steps needed for the construction of the final segments.

Electrolyte and fluid secretion by cultured human inner medullary collecting duct cells.

Inner medullary collecting ducts (IMCD) are the final nephron segments through which urine flows. To investigate epithelial ion transport in human IMCD, we established primary cell cultures from initial (hIMCD(i)) and terminal (hIMCD(t)) inner medullary regions of human kidneys. AVP, PGE(2), and forskolin increased cAMP in both hIMCD(i) and hIMCD(t) cells. The effects of AVP and PGE2 were greatest in hIMCD(i); however, forskolin increased cAMP to the same extent in hIMCD(i) and hIMCD(t). Basal short-circuit current (I(SC)) of hIMCD(i) monolayers was 1.4 +/- 0.5 microA/cm2 and was inhibited by benzamil, a Na+ channel blocker. 8-Bromo-cAMP, AVP, PGE(2), and forskolin increased I(SC); the current was reduced by blocking PKA, apical Cl- channels, basolateral NKCC1 (a Na+ - K+ - 2Cl- cotransporter), and basolateral Cl-/HCO(3)(-) exchangers. In fluid transport studies, hIMCD(i) monolayers absorbed fluid in the basal state and forskolin reversed net fluid transport to secretion. In hIMCD(t) monolayers, basal current was not different from zero and cAMP had no effect on I(SC). We conclude that AVP and PGE2 stimulate cAMP-dependent Cl- secretion by hIMCD(i) cells, but not hIMCD(t) cells, in vitro. We suggest that salt secretion at specialized sites along human collecting ducts may be important in the formation of the final urine.