Fimbriate Margin Research Articles

Cocconeiscrisscrossis sp. nov., a new monoraphid diatom (Bacillariophyta) from southern China.

A novel monoraphid diatom species, Cocconeiscrisscrossis You, Yu, Kociolek & Wang, sp. nov. is examined and described from the Qingyi River and Maolan Nature Reserve of southern China. The morphological description is based on light microscopy and scanning electron microscopy observations and the new species is compared with similar taxa in this genus. The characteristics unique to Cocconeiscrisscrossissp. nov. include its central area extending irregularly to both sides, it having closed valvocopulae with heavily silicified fimbriate margins and poles of the valvocopulae have 'sword-shaped' siliceous extensions. These features differentiate this new species from others in the genus. This new species was found in alkaline waterbodies, including streams, waterfall and ponds. It was usually found as an epiphyte on the stones; however, it was present on other substrates such as mosses.

Revision of Thraulus Eaton 1881 (Ephemeroptera: Leptophlebiidae: Choroterpinae).

The genus Thraulus is widespread throughout much of the Eastern Hemisphere. Since Eaton established Thraulus in 1881, 62 species have, at one time or another, been placed in this genus. Thirty-eight of those species were eventually moved to other genera. Any comprehensive study of the remaining species, based on the published literature, is difficult as they were described by many authors, using different criteria, over a period of 142 years. The purpose of this study was to redescribe this genus, based on previously described species and nine new species, and to provide a format for future taxonomic and morphological studies of Thraulus. Redescriptions of most species were based on direct examination of external morphological characters. Descriptions or diagnoses of species, whose types were unavailable for study, were made using the original published description and additional information provided by authors of several of those species. The following species were studied: Thraulus amravati Vasanth, Subramanian & Selvakumar, 2022; T. bellus Eaton, 1881; T. bishopi Peters & Tsui, 1972; T. cuspidatus Vasanth, Subramanian & Selvakumar, 2022; T. demoulini Peters & Tsui, 1973; T. fasciatus (Kimmins, 1956); T. fatuus Kang & Yang, 1994; T. femoratus Li, Liu & Zhou, 2006; T. gopalani Grant & Sivaramakrishnan, 1985; T. jacobusi Isack, Srinivasan, Sivaruban & Barathy, 2022; T. macilentus Kang & Yang, 1994; T. malabarensis Vasanth, Subramanian & Selvakumar, 2022; T. mudumalaiensis Soman, 1991; T. plumeus Selvakumar, Vasanth & Subramanian, 2022; T. semicastaneus (Gillies, 1951); T. thiagarajani Balasubramanian & Muthukatturaja, 2019; T. thraker Jacob, 1988; T. torrentis (Gillies, 1964); T. turbinatus (Ulmer, 1909); T. umbrosus Kang & Yang, 1994; and T. vellimalaiensis Vasanth, Subramanian & Selvakumar, 2022. Nine new species of Thraulus are described: T. connubialis sp. nov., Malaysia; T. cursus sp. nov., Japan; T. eatoni sp. nov., Indonesia; T. ishiwatai sp. nov., Japan; T. madagasikarensis sp. nov., Madagascar; T. nihonensis sp. nov., Japan; T. opifer sp. nov., Australia; T. parentalis sp. nov., Malaysia; and T. petersorum sp. nov., Malaysia. Thraulus can be distinguished from all other genera of Leptophlebiidae by the following combination of characters: In the imagos, 1) upper portion of eyes oval-suboval, major axes diverge anteriorly; 2) vein MA fork of fore wings symmetrical; 3) vein MP fork of fore wings asymmetricala cross vein connects base of MP2 to MP1, MP fork closer to base of wing than Rs fork; 4) strongly oblique cross vein extends between veins R4+5 and MA1 just apical to fork of vein MA; 5) 2 cubital intercalary veins in fore wings; 6) costal projection on hind wings well-developed, bluntly rounded to acutely pointed; 7) claws dissimilarone blunt and pad-like, the other apically hooked; 8) penes long, relatively straight, narrow, parallel, usually contiguous mesally but not fused, apex may have lateral projections; 9) sternum 7 of female with posterior margin straight or shallowly concave or convex mesally; and 10) sternum 9 of females rounded apically. In addition, penile spines occur on most species. In the nymphs, 1) lateral margins of clypeus parallel; 2) width of labrum subequal to width of clypeus; 3) 2 dorsal rows of setae on labrum; 4) venter of labrum with 1 row of short stout setae on either side of midline near anterior margin, rows curve mesally; 5) hypopharynx with small, rounded, posterolateral projections on arms of superlingua; 6) large spine on posterolateral corners of terga 69, 79 or 89; 7) gills 17 dissimilar: gill 1 composed of 1 or 2 subulate lamellae or a dorsal subulate lamella and a ventral fimbriate oval lamella, and gills 27 composed of dorsal and ventral oval lamellae with fimbriate margins. Two species continue to be nomen dubiumT. siewertii (Weyenbergh, 1883) and T. vogleri (Weyenbergh, 1883). Thraulus grandis Gose, 1980 is considered nomen nudum. A review of published phylogenetic studies involving Thraulus is provided. With the species discussed in this paper, along with reports of additional new species to be described, Thraulus has the potential to be included among the more specious genera of Ephemeroptera.

First record of Epidendrum ×doroteae (Orchidaceae) in South America

Epidendrum ×doroteae (Orchidaceae), a natural hybrid between Epidendrum ciliare and Epidendrum nocturnum, was hitherto known only from a few specimens collected during the 1940s–1990s in Honduras, Central America. Epidendrum ×doroteae is recorded here for the first time in Nicaragua and in South America, in Brazil. We found three clusters of individuals of the hybrid in sympatry with the parental species on Marajó Island, state of Pará, in the Brazilian Amazon, and we recognised two specimens from Nicaragua deposited in the herbaria AMO and MO as E. ×doroteae. Epidendrum ×doroteae and the parental taxa have star-shape flowers with yellowish-green sepals and petals. However, E. ×doroteae differs from the parental species when fertile by the lateral lobes of the lip, which have an irregularly serrate-dentate, rarely slightly fimbriate margin vs. entire to slightly undulate margin in E. nocturnum and distinctly fimbriate margin in E. ciliare.

Molecular Systematics and Taxonomic Analyses of Three New Wood-Inhabiting Fungi of Hyphoderma (Hyphodermataceae, Basidiomycota).

In this present study, three new wood-inhabiting fungal taxa, Hyphoderma niveomarginatum, H. sordidum and H. weishanense, are proposed. Hyphoderma niveomarginatum is characterized by the ceraceous basidiomata having a smooth, cracking hymenial surface and the presence of the moniliform cystidia and ellipsoid basidiospores (7-9 × 3.5-5 µm). Hyphoderma sordidum is characterized by its resupinate basidiomata with a smooth hymenial surface with the fimbriate margin, the presence of the tubular cystidia and ellipsoid basidiospores (3-4.5 × 2-3 µm). Hyphoderma weishanense differs in its membranous basidiomata with a slightly buff to buff hymenial surface and the presence of broadly ellipsoid basidiospores (4.5-8.5 × 4-7 µm). Sequences of ITS+nLSU+mt-SSU+RPB1+RPB2 genes were used for the phylogenetic analyses using three methods. The ITS+nLSU+mt-SSU+RPB1+RPB2 analysis of the genus Hyphoderma indicated that the 3 new species of Hyphoderma were nested into genus Hyphoderma, in which H. niveomarginatum formed a single group and then grouped with H. membranaceum and H. sinense; H. sordidum was a sister to H. nudicephalum; and H. weishanense closely grouped with H. crystallinum.

First report of Curvularia muehlenbeckiae, the causal agent of Curvularia leaf spot on pecan (Carya illinoinensis) in China.

The pecan (Carya illinoinensis) industry is largely affected by the increased incidence of diseases (Xiao et al 2021). Leaf spot symptoms were identified in an orchard of cultivar Pawnee pecan trees at Zhejiang A&F University, Zhejiang, China in August 2020. Small black spots occurred on the veins and edges of the leaves and nearby tissues turned yellow and slightly deformed (May to July). The spots (0.5-1.5cm) spread to all leaves with 25% to 40% leaf drop occurring in almost all trees between August and October. The causal pathogen was isolated from leaves with target symptoms using the following method: surface sterilized with 70% alcohol (2×, 30 s), rinsed with sterilized water (3×), leaf spots excised and placed on PCA media, and left to incubate at 28℃ in the dark for 3-d. Mycelium on the edge of each clone was excised and incubated on fresh oatmeal agar medium with a 12-hour light/dark cycle for 7-d to obtain conidia. Single spore isolates were germinated on PDA medium under the same conditions as previously described, one 5-mm hyphal plug was transferred to fresh PCA media to obtain the pure cultures. The pathogenicity of the isolates were verified using Koch's postulates. Non-wounded healthy leaves (disinfected with 1% NaClO) of cv. Pawnee (disease susceptible) were obtained from seedlings grown in green-house at 26 ℃ and inoculated with 5-mm hyphal plugs and a conidia-hyphae suspension (~106/mL) containing one-week old purified cultures. After 3-15 days post-inoculation, small black spots appeared on the leaves inoculated with isolate P-6 (the only pathogenic isolate from the leaf spots in the orchard) and grew larger until the whole leaf wilted while the control leaves remained asymptomatic. The experiment was repeated two times with two bio-replicates each run. Finally, the pathogen was re-isolated from infected leaves, which showed the same symptoms as the previous isolate. Aerial mycelia of P-6 turned from white to gray and substrate mycelia from brown to black. Colonies had a fimbriate margin before mycelia filled the medium. Hyphae were septate, branched, brown or black, smooth wall and 1.4-10 µm in width. Conidiophore single-branch, dark brown, curved or straight, 1.93-5.51×44.12-104.41 μm width, conidiogenous cells 6.66-16.67 µm (terminal) and 8.82-23.33 µm (intercalary) length, mono- to polytretic, proliferating sympodially. Conidia (n=20) four cells, 15.7 - 25.7 μm × 7.1 - 11.4 μm wdth, swelling and curving from the basal cell to the third. The bending angle was 5° to 80°. The middle two cells were brown and usually verruculose, the basal and apical cells paler and less ornamented. No sexual morph observed. The morphological characteristics matched previous descriptions of Curvularia species (Madrid et al 2014). The identity of P-6 was confirmed by phylogenetic reconstruction using the concatenated sequences of ITS rDNA, partial GAPDH, LSU, TEF-1α and RPB2 regions (Raza et al 2019). The genomic DNA of P-6 was extracted by the M5 hipermix-MF859 (Mei5 Biotechnology). The sequences of P-6 were used for nucleotide BLAST against the Standard databases and model strains were selected to construct the concatenated sequences of GAPDH-ITS-LSU-TEF-1α-RPB2 for building a phylogenetic tree. This analysis identified P-6 as a strain of C. muehlenbeckiae, a species with few reports other than in gramineous crops (Raza et al 2019; Chen et al 2021; Cui et al 2020; Ni et al 2016). This is the first report of C. muehlenbeckiae on pecan in China and worldwide.

Lamprospora benkertii sp. nov., and an evaluation of Lamprospora spp. with seaveri-type ascospore ornamentation

Lamprospora benkertii is described as a new species based on collections from Georgia, Germany, Norway, Slovakia, and Switzerland. It is characterized by orange apothecia without a fimbriate margin, globose ascospores with a seaveri -type ornamentation comprising strong ridges forming a reticulum and a secondary reticulum formed by fine ridges within the meshes of the primary reticulum. The fungus infects rhizoids of Trichostomum crispulum. Delineation of the new species is supported by LSU and EF1α sequence analyses. Lamprospora benkertii is compared with other Lamprospora species with a seaveri -type or similar ascospore ornamentation: L. ascoboloides, L. cailletii, L. dicranellae, L. funigera, L. hispanica, L. irregulariata, L. leptodictya, L. norvegica, L. paechnatzii, L. seaveri. We also present an updated key for this group of species as well as a more complete description of L. hispanica based on our observations from several collections. New host specificity data are provided for L. hispanica, L. leptodictya, L. norvegica, and L. paechnatzii.

Salvia fimbriaticalyx, a new species of Salvia (Lamiaceae) from Oaxaca, Mexico

In this study, we describe and illustrate Salvia fimbriaticalyx, a new species from Oaxaca, Mexico, which is morphologically similar to Salvia leptostachys, a species from section Angulatae. The new species differs from the latter by having persistent, lanceolate bracts with cuneate base; calyx lobes with a densely fimbriate margin; corolla tube longer (3–3.5 mm long), and the lower lip of the corolla shorter (3–3.5 mm long). Three molecular markers (ITS, trnL-trnF and trnH-psbA) were sequenced for the new species, and its phylogenetic position is discussed.

Surirella dongtingensis sp. nov. and ultrastructure of S. undulata (Bacillariophyta) from China

ABSTRACT The morphological diversity in the genus Surirella is summarized, a new Surirella species is described, and the valve ultrastructure of S. undulata is documented under the scanning electron microscope. The new species, Surirella dongtingensis sp. nov., has a moderately twisted frustule with open valvocopula. Under the light microscope the frustule has one valve that is twisted at one apex, and the second valve is rotated 180 degrees compared to the first, such that one with the left side of one apex is not in the same focal plane with the three other sides, and the second is that the right side of one apex is not in the same focal plane with the three other sides. With respect to the valvocopula, the pars exterior bears oblique striae and scattered warts, and the pars interior have parallel striae and fimbriate ad-valve margins. Internally, both pars exterior and interior are smooth. Two nomenclatural changes for Cymatopleura aquastudia Kociolek & Q.You and C. xinjiangiana Q.You & Kociolek are provided to account for species to align with current concepts of the genus Surirella.

Stemona fimbritepala (Stemonaceae), a new species from northern Laos

Stemona fimbritepala (Stemonaceae), a new species from northern Laos, is described and illustrated. It is morphologically similar to S. hirtella and S. kerrii but clearly differs in longer peduncle, longer tepals, longer petaloid outgrowth of the connective and fimbriate margin of inner tepals. A detailed description, distribution, ecology, etymology, preliminary conservation status and photographs of this species are provided. A key to discriminate between the three hairy species is presented.

Two new species of Phyllanthus (Phyllanthaceae) from Thailand.

Two Phyllanthus species are newly described from a limestone mountain in the north of Thailand. The first species, P. huamotensis Pornp., Chantar. & J.Parn., sp. nov., is one of the most distinct Phyllanthus species easily distinguished by its reddish branchlets and stem, conspicuous reddish venation, especially on the lower leaf surface, red sepals with long fimbriate margin and red capsule with papillose-puberulous surface. The second species, P. chantaranothaii Pornp., J.Parn. & Hodk., sp. nov., is similar to P. pulcher Wall. ex Müll.Arg., but it is distinguished by its puberulous upper leaf surface and pistillate flowers which have red, narrowly lanceolate sepals with a white, long fimbriate margin, puberulous outer side as well as puberulous pedicel.

<p><strong><em>Marasmiellus griseobrunneus</em></strong><strong> <em>sp. nov.</em> (Omphalotaceae) from Kerala State, India</strong></p>

Marasmiellus griseobrunneus sp. nov. is described from Kerala State, India, on the basis of both morphology and molecular phylogeny. This species is characterized by a grayish brown, sulcate-striate pileus with a fimbriate margin, weakly to strongly intervenose lamellae, pip-shaped to short lanceolate or lacrymoid basidiospores, versiform cheilo- and pleurocystidia, subregular hymenophoral trama, and the presence of appressed or ascending setae on the pileipellis and stipitipellis. A detailed description, comparison with both morphologically and phylogenetically related species, photographs of both the basidiocarps and the microscopic structures, and a Maximum Likelihood phylogram, based on the sequence of the Internal Transcribed Spacer region of the nuclear ribosomal DNA, depicting the placement of the new species are provided.

<p><strong>A new species of <em>Polycarpaea </em>(Caryophyllaceae) from India</strong></p>

Polycarpaea rangaiahiana, a new species of Caryophyllaceae from the lateritic plains of Kannur District, Kerala, India is described and illustrated. P. rangaiahiana is morphologically similar to P. aurea from which differs in having ovate-lanceolate stipules with fimbriate margin and other floral characters. The name P. aurea is discussed from the nomenclatural point of view and a second-step typification was proposed.

Drymonia croatii (Gesneriaceae), a new species from the Pacific slopes of the Colombian Andes

Drymonia croatii, a new species of Gesneriaceae from the Pacific slopes of the Colombian Andes (department of Valle del Cauca) is described and illustrated. Drymonia croatii is similar to D. ovatifolia and D. foliacea; it is differentiated by the following characters: indument on stem and leaves composed of a combination of simple (septate and non-septate) and glandular hairs; peltate glandular hairs on the abaxial surfaces of leaf blades, inflorescence bracts and calyx; corolla tube white with purple veins outside and a yellow ventral canal inside, basal third of the corolla tube narrow, corolla lobes purple with fimbriate margins; and a fleshy capsule with reflexed valves that are light yellow with brown dots adaxially.

Lanlabeo duanensis, a new genus and species of labeonin fish (Teleostei: Cyprinidae) from southern China.

Lanlabeo, a new genus of fish belonging to the family Cyprinidae, is described from the Pearl River drainage basin in Guangxi Province, southern China. This new genus is distinguished from all other genera of the cyprinid tribe Labeonini by a combination of morphological and phylogenetic characters. It differs morphologically from all other Asian labeonins in its uniquely modified oromandibular morphology. For example, this genus has a frenum connecting the upper jaw and lower lip at the corner of the mouth, regularly arranged papillae densely scattered over the ventral margin of the rostral cap, a vestigial upper lip, a rostral cap overlying the upper jaw and with a fimbriate posterior margin, and a lower lip divided into two lateral fleshy lobes and one central plate. These two lateral fleshy lobes are small and translucent, and the median lobe of the lower lip is large and has papillae regularly arranged in many transverse rows. In the lower jaw, the dentary is transversely L-shaped in ventral view because its anterior part forms a right-angle turn and its transverse branch is anterioposteriorly expanded. In addition, analyses of four nuclear gene datasets indicate that this new genus forms a highly divergent lineage within the Labeonini, and that Lanlabeo is closely related to the genus Ptychidio. Therefore, based on morphometric differences and phylogenetic relationships, we describe this new genus herein as Lanlabeo, containing the new species Lanlabeo duanensis.

Lamprospora sylvatica (Pyronemataceae), a new bryophilous ascomycete on Dicranum montanum

Lamprospora sylvatica is described as a new species based on finds from Ukraine, Slovakia, Germany and Norway. It is characterised by the combination of the following features: pinkish, orange to reddish-orange apothecia with a fimbriate margin, globose ascospores with more or less regular areolate ornamentation, infecting strong rhizoids of Dicranum montanum with an infectious structure consisting of a one-celled appressorium surrounded by a multi-layered cluster of thick-walled cells and haustorium within the rhizoids. The apothecia were always found on rotten wood, which is an unusual habitat for hosts of bryophilous Pezizales. The new species is compared to similar taxa morphologically and by means of DNA sequencing. In the phylogenetic analysis based on LSU and ITS regions, L. sylvatica forms a well-supported clade close to L. feurichiana (on Ceratodon purpureus), L. kristiansenii (also on C. purpureus) and L. campylopodis (on Campylopus spp.).

First record of Geosiris (Iridaceae: Geosiridoideae) from Australasia : a new record and a new species from the Wet Tropics of Queensland, Australia

Gray, B. & Y.W. Low (2017). First record of Geosiris (Iridaceae: Geosiridoideae) from Australasia: a new record and a new species from the Wet Tropics of Queensland, Australia. Candollea 72: 249–255. In English, English abstract.Geosiris Baill. is a small genus of achlorophyllous, mycoheterotrophic herbs that until now consisted of only two species, Geosiris albiflora Goldblatt & J.C. Manning and Geosiris aphylla Baill., the latter being the type species of the genus. Prior to this study, the genus was known only from two islands off the southeast coast of Africa, Madagascar and Mayotte. A recent discovery in Australia reported here expands its geographic range to the Pacific. The Australian taxon represents a species distinct from the two African taxa based on the key morphological characters for species distinction in the genus, namely stigma characteristics. Geosiris albiflora has a somewhat club-like stigma with three coherent lobes, Geosiris aphylla with a stigma terminates in three fringed broad and flat stigmatic lobes, and the Australian Geosiris has a truncate stigma with a short fimbriate margin. Hence, the Australian taxon is formally described here as Geosiris australiensis B. Gray & Y.W. Low.Received: March 3, 2017; Accepted: April 14, 2017; First published online: June 24, 2017

Osmophore diversity in the Catasetum cristatum Lindl. alliance (Orchidaceae: Catasetinae)

Catasetum is a neotropical orchid genus of 130 species characterized by its unisexual flowers. They are pollinated by male Euglossini bees. It is widely know that these bees collect volatile compounds in Catasetum from structures called osmophores. However, there is little information on morpho-anatomy and histochemistry of secretory tissues for this neotropical genus and data are lacking. Based on these arguments members of the Catasetum cristatum alliance, namely C. arietinum, C. ariquemense, C. barbatum, C. carolinianum, C. cristatum, C. lanciferum, C. multifidum, C. multifissum, C. rivularium and C. semicirculatum were analyzed. The labellum of male flowers of this alliance is elongate and with fimbriate margins, possessing two protuberances and a median saccate portion. The labellum of female flowers is galeiform and there is no ornamentation. The entire adaxial surface of the labellum is secretory in both sexes, including the fimbriae of male flowers. The structure of the secretory tissue is similar among species, and they are composed of a simple epidermis and five layers of underlying parenchyma. In most species the epidermis is flat, and cells are elongated in the saccate portion. In C. ariquemense and C. carolinianum the epidermal cells are papilous, while in C. semicirculatum they have convex surface (male flowers). The histochemical analysis detected lipophilic droplets and starch grains inside the secretory cells. All characteristics observed are encountered in secretory tissues with high-energy demand, as is common in osmophores.

Una especie nueva de Alsobia (Gesneriaceae) de Chiapas, México

Alsobia chiapensis, a new species from Chiapas, Mexico is described and illustrated. It differs from its 2 congeners by a relatively larger corolla and shorter limb; linear-lanceolate calyx lobes; and petiole less than a third of the length of the leaf blade. The new species shares characteristics found in other species of Alsobia such as fimbriate margins on the corolla lobes, and stolons.

Woodsia guizhouensis(Woodsiaceae), a New Species from a Limestone Area in Guizhou, China

A new pteridophyte species, Woodsia guizhouensis P. S. Wang, Q. Luo & Li Bing Zhang (Woodsiaceae), is described and illustrated from higher elevational areas in Guizhou, China. It is morphologically most similar to W. andersonii (Bedd.) Christ and W. rosthorniana Diels. Woodsia guizhouensis is only 3–8 cm tall, its rhizome scales are light brown with sparsely fimbriate margins, its pinnae are short-petiolulate, and its pinna bases are cordate or sometimes rounded. The perispore sculpture of W. guizhouensis pollen is cristate, with numerous acanthoid projections and a few irregular perforations. Woodsia guizhouensis is only known from four localities in western Guizhou and is considered to be Endangered (EN), based on IUCN Red List criteria.

Two new species of Steccherinum (Basidiomycota, Polyporales) from Taiwan

Steccherinum cremicolor and S. elongatum are described and illustrated as new species from Taiwan. Steccherinum cremicolor is characterized by strictly resupinate basidiocarps, a fimbriate margin, short spines, generative hyphae dominating in the trama and subiculum, encrusted skeletocystidia, and ellipsoid basidiospores. Steccherinum elongatum has strictly resupinate and membranaceous basidiocarps, bearing fairly long spines, generative hyphae-dominated subiculum, elongated skeletocystidia, and ellipsoid to subglobose basidiospores.