This research uses electrospinning to present a method for producing a pressure- and breath-responsive nanofiber membrane (PBAT/PDMS/CNTsNFM). This method utilizes polydimethylsiloxane (PDMS) and poly (butylene adipate-co-terephthalate) (PBAT), which is biodegradable, as the matrix, along with carbon nanotubes (CNTs) as a conductive filler. Additionally, a combination approach was proposed incorporating cellulose non-woven fabric (CNWF) as a support and initial filtration layer, serving as a collector for electrospinning PBAT/PDMS/CNTsNFM to create a twin-layer structure. In rheology analysis, the viscosity of PBAT/PDMS/CNTs solution was 0.811 Pa·s at the shear rate of sample preparation. PBAT/PDMS/CNTs solution performed 30.14 mN/m in the surface tension investigation. The formation mechanism of PBAT/PDMS/CNTsNFM was analyzed, and CNTs aid in forming fibers in PBAT/PDMS/CNTsNFM. PBAT/PDMS/CNTsNFM exhibits an average fiber diameter of 370 nm. In the air purification performance test (with PM0.3, 85 L/min air flow rate, 8 mg/m3 PM concentration), the 7.62 g/m2 PBAT/PDMS/CNTsNFM sample performed an air purification efficiency of 87.5 % and a pressure drop of 225 Pa, and the 22.3 g/m2 PBAT/PDMS/CNTsNFM sample performed an air purification efficiency of 92.4 % and a pressure drop of 1959 Pa. In the pressure detection test, the resistance of the 22.3 g/m2 PBAT/PDMS/CNTsNFM sample decreased as the pressure increased, and the response can be observed even at a low pressure of 25 Pa. The 7.62 g/m2 PBAT/PDMS/CNTsNFM sample could respond to breath with different strengths. The CNTs form a pressure-constructed dynamic conductive network in PBAT/PDMS/CNTsNFM. PBAT/PDMS/CNTsNFM is expected to be applied to pressure sensing, monitorable air purification, and breathing detection due to its performance in pressure detection and air purification.