Filament Spacing Research Articles

Simulation Study on Airflow Field Uniformity of HFCVD Deposition System by Filament Spacing

Simulation Study on Airflow Field Uniformity of HFCVD Deposition System by Filament Spacing

Filamentary surface plasma discharge flow length and time scales

Nanosecond surface dielectric barrier discharges (ns-SDBDs) are a class of plasma actuators that utilize a high-voltage pulse of nanosecond duration between two surface-mounted electrodes to create an electrical breakdown of air, along with rapid heating. These actuators usually produce multiple filaments when operated at high pulse frequencies, and the rapid heating leads to the formation of shock waves and complex flow fields. In this work we replicate a single filament of the ns-SDBDs and characterize the induced flow using velocity measurements from particle image velocimetry and density measurements from background-oriented schlieren. The discharge is produced by a high voltage electrical pulse between two copper electrodes on an acrylic base. A hot gas kernel characterizes the flow field formed close to the electrodes that expands and cools over time and a vortex ring that propagates away from the surface while entraining cold ambient fluid. The gas density deficit inside the kernel displays a power-law decay over time. Based on the observations, we develop a simplified theoretical model based on vortex-driven cooling and perform a scaling analysis to obtain the induced flow length and time scales. The results show that the cooling process’s time scales correspond to a circulation-based time scale of the vortex ring, and the length scale of the kernel corresponds to the vortex ring radius. These findings can guide the choice of optimal filament spacing and pulse frequencies in the design, deployment, and operation of ns-SDBDs for flow control.

Design and Fabrication of Flexible Capacitive Sensor With Cellular Structured Dielectric Layer via 3D Printing

Because of high sensitivity, mechanical robustness, lightweight and wearability, flexible capacitive pressure transducer has been widely considered one of the most critical soft electronics in wearable consumables and e-skins. The enhancement of the pressure sensitivity of a flexible capacitive sensor relies on the introduction of interfacial microstructure to the dielectric layer. We demonstrate a new methodology to fabricate flexible capacitive sensors with copper-plated polyimide (PI) films as the electrodes and a porous polydimethylsiloxane (PDMS) layer 3D printed via the direct-ink-writing approach. Time-of-flight secondary ion mass spectrometry is developed to optimize the electroless copper plated PI films. What is further examined is the impact of the geometric complexity of the cellular PDMS structure, including filament width, spacing and alignment, on sensitivity, repeatability and reliability of the developed capacitive sensor. A robotic gripper equipped with our flexible pressure sensor showcases its competence to grip a soft target with well-posed force control. It is expected that our proposed sensor design and manufacturing methodology will advance the development of soft electronics and wearable sensors.

Cell-Free Bilayered Porous Scaffolds for Osteochondral Regeneration Fabricated by Continuous 3D-Printing Using Nascent Physical Hydrogel as Ink.

Cartilage is difficult to self-repair and it is more challenging to repair an osteochondral defects concerning both cartilage and subchondral bone. Herein, it is hypothesized that a bilayered porous scaffold composed of a biomimetic gelatin hydrogel may, despite no external seeding cells, induce osteochondral regeneration in vivo after being implanted into mammal joints. This idea is confirmed based on the successful continuous 3D-printing of the bilayered scaffolds combined with the sol-gel transition of the aqueous solution of a gelatin derivative (physical gelation) and photocrosslinking of the gelatin methacryloyl (gelMA) macromonomers (chemical gelation). At the direct printing step, a nascent physical hydrogel is extruded, taking advantage of non-Newtonian and thermoresponsive rheological properties of this 3D-printing ink. In particular, a series of crosslinked gelMA (GelMA) and GelMA-hydroxyapatite bilayered hydrogel scaffolds are fabricated to evaluate the influence of the spacing of 3D-printed filaments on osteochondral regeneration in a rabbit model. The moderately spaced scaffolds output excellent regeneration of cartilage with cartilaginous lacunae and formation of subchondral bone. Thus, tricky rheological behaviors of soft matter can be employed to improve 3D-printing, and the bilayered hybrid scaffold resulting from the continuous 3D-printing is promising as a biomaterial to regenerate articular cartilage.

X-ray diffraction and second harmonic imaging reveal new insights into structural alterations caused by pressure-overload in murine hearts

We demonstrate a label-free imaging approach to study cardiac remodeling of fibrotic and hypertrophic hearts, bridging scales from the whole organ down to the molecular level. To this end, we have used mice subjected to transverse aortic constriction and imaged adjacent cardiac tissue sections by microfocus X-ray diffraction and second harmonic generation (SHG) imaging. In this way, the acto-myosin structure was probed in a spatially resolved manner for entire heart sections. From the recorded diffraction data, spatial maps of diffraction intensity, anisotropy and orientation were obtained, and fully automated analysis depicted the acto-myosin filament spacing and direction. X-ray diffraction presented an overview of entire heart sections and revealed that in regions of severe cardiac remodeling the muscle mass is partly replaced by connective tissue and the acto-myosin lattice spacing is increased at these regions. SHG imaging revealed sub-cellular structure of cardiac tissue and complemented the findings from X-ray diffraction by revealing micro-level distortion of myofibrils, immune cell infiltration at regions of cardiac remodeling and the development of fibrosis down to the scale of a single collagen fibril. Overall, our results show that both X-ray diffraction and SHG imaging can be used for label-free and high-resolution visualization of cardiac remodeling and fibrosis progression at different stages in a cardiac pressure-overload mouse model that cannot be achieved by conventional histology.

Nondestructive evaluation of fused filament fabrication 3D printed structures using optical coherence tomography

PurposeThe quality of components under fused filament fabrication (FFF) is related to the correct filament spacing and bonding of successively deposited layers and is evaluated mainly by scanning electron microscopy (SEM). However, it is a destructive technique and real-time evaluation is not possible. Optical coherence tomography (OCT), on the other hand, is an optical method that acquires cross-sectional images non-invasively and in real-time. Therefore, this paper aims to propose and validate the use of OCT as a non-destructive quality evaluation tool for FFF using Polylactic Acid (PLA) filaments.Design/methodology/approachPLA three-dimensional (3D) printed samples were made in a variety of nozzle temperatures and mesh spacing. These samples were fractured in liquid nitrogen and inspected using SEM (as a gold standard) to evaluate dimensions and morphology, then the samples were evaluated by OCT in the same area, allowing the results confrontation.FindingsOur results indicate a good correlation between OCT and SEM for the dimensional assessment of layers. When the filament was extruded in lower temperatures, the OCT images presented sharply defined interfaces between layers, in contrary to higher nozzle temperatures, denoting better fusion between them. However, higher extruding temperatures are incurred in greater deviations from nominal dimensions of the mesh. Finally, we demonstrate the advantage of a full 3D tomographic reconstruction to inspect within a FFF sample, which enabled the inspection of “hidden” information, not visible on a single cross-sectional cut.Originality/valueThis paper proposes OCT as a novel and nondestructive evaluation tool for FFF.

Biofabrication of multiscale bone extracellular matrix scaffolds for bone tissue engineering.

Interconnected porosity is critical to the design of regenerative scaffolds, as it permits cell migration, vascularisation and diffusion of nutrients and regulatory molecules inside the scaffold. 3D printing is a promising strategy to achieve this as it allows the control over scaffold pore size, porosity and interconnectivity. Thus, the aim of the present study was to integrate distinct biofabrication strategies to develop a multiscale porous scaffold that was not only mechanically functional at the time of implantation, but also facilitated rapid vascularisation and provided stem cells with appropriate cues to enable their differentiation into osteoblasts. To achieve this, polycaprolactone (PCL) was functionalised with decellularised bone extracellular matrix (ECM), to produce osteoinductive filaments for 3D printing. The addition of bone ECM to the PCL not only increased the mechanical properties of the resulting scaffold, but also increased cellular attachment and enhanced osteogenesis of mesenchymal stem cells (MSCs). In vivo, scaffold pore size determined the level of vascularisation, with a larger filament spacing supporting faster vessel in-growth and more new bone formation. By freeze-drying solubilised bone ECM within these 3D-printed scaffolds, it was possible to introduce a matrix network with microscale porosity that further enhanced cellular attachment in vitro and increased vessel infiltration and overall levels of new bone formation in vivo. To conclude, an "off-the-shelf" multiscale bone-ECM-derived scaffold was developed that was mechanically stable and, once implanted in vivo, will drive vascularisation and, ultimately, lead to bone regeneration.

Numerical simulations for fluid dynamics and temperature patterns in membrane distillation channels

The effect of spacer orientation on flow behavior is studied at different spacer filament spacings using Computational Fluid Dynamics (CFD) technique. At high inlet velocity / Reynolds number the flow becomes transient and vorticity magnitude increases in a major portion of the two channels. The temperature and heat flux in this case also vary in time. The comparison of various spacer geometrical arrangements/orientations shows that the arrangements in which the spacer filaments are opposite to the membrane layers are more suitable due to higher heat transfer rates. Further appropriate turbulence models for predicting flow and heat transfer behavior in membrane channels are also proposed.

Experimentally-validated computational simulation of direct contact membrane distillation performance

A Direct Contact Membrane Distillation system using a flat-sheet membrane module was fabricated to experimentally study the effects of operating conditions (inlet temperatures and flow rates of feed and permeate streams and salt concentration) on its distilled water production. A two-dimensional Computational Fluid Dynamics (CFD) model considering transmembrane mass flux and heat conduction and associated phase change heat transfer (evaporation and condensation) was developed. It was found that the computational predictions of distilled water production agreed well with the experimental measurements. Furthermore, the CFD model analyzed the effects of filament spacing of a screen spacer in the flow channels and flow configuration (counterflow and parallel) on the water distillation performance. In this study, it is concluded that high water distillation is achieved by using low permeate temperature, high feed temperature, high feed and permeate flow rates, and less salt concentration in a counterflow configuration. The water distillation is more influenced by the feed temperature than the permeate temperature due to the water saturation pressure-temperature relation. The simulation results also showed that the convection thermal resistances in the feed and permeate channels are decreased by using screen spacers and therefore, the temperature and concentration polarization effects are decreased in the channels.

Fabricating ceramics with embedded microchannels using an integrated additive manufacturing and laser machining method

AbstractIn this paper, we demonstrate the feasibility of an innovative method to fabricate flexibly embedded microchannels in bulk ceramics of complex geometries. This innovative method is an integration of extrusion freeforming (EFF) and picosecond (PS) laser machining. The bulk ceramic green body of complex geometries was fabricated using the extrusion method. After one green layer was extruded, in‐plane microchannels with variable cross‐section sizes and aspect ratios were fabricated using a picosecond laser. After the microchannels were fabricated, a cover layer was extruded. The green state processed structures ceramics were pressurelessly sintered to a bulk density of ~94%. In this paper, sinuous channels, channel networks, and patterns have been demonstrated. Vertical channels can also be drilled using the picosecond laser layer‐by‐layer with uniform diameters. The wall between the neighboring channels can be as thin as ~60 μm without any observable defects after firing. With the correct paste rheology, the cover layer did not sag into the microchannels and the bonding between layers was excellent. We did not observe any debonding between layers. During extrusion, the gaps between the adjacent filaments can be eliminated by controlling the filament spacing and the distance between needle tip and substrate. After firing, microchannels did not distort or collapse. The laser can cut through multiple layers without damage to the bonding between layers. Due to the uniform shrinkage during pressureless sintering, the green shapes of the microchannels were well preserved.

On the Numerical Robustness of the Streaming Instability: Particle Concentration and Gas Dynamics in Protoplanetary Disks

Abstract The streaming instability (SI) is a mechanism to concentrate solids in protoplanetary disks. Nonlinear particle clumping from the SI can trigger gravitational collapse into planetesimals. To better understand the numerical robustness of the SI, we perform a suite of vertically stratified 3D simulations with fixed physical parameters known to produce strong clumping. We vary the numerical implementation, namely, the computational domain size and the vertical boundary conditions (vBCs), comparing newly implemented outflow vBCs to the previously used periodic and reflecting vBCs. We find strong particle clumping by the SI is mostly independent of the vBCs. However, peak particle densities are higher in larger simulation domains due to a larger particle mass reservoir. We report SI-triggered zonal flows, i.e., azimuthally banded radial variations of gas pressure. These structures have low amplitudes, insufficient to halt particle radial drift, confirming that particle trapping in gas pressure maxima is not the mechanism of the SI. We find that outflow vBCs produce artificially large gas outflow rates at vertical boundaries. However, the outflow vBCs reduce artificial reflections at vertical boundaries, allowing more particle sedimentation, and showing less temporal variation and better convergence with box size. The radial spacing of dense particle filaments is ∼0.15 gas scale heights (H) for all vBCs, which sets the feeding zone for planetesimal growth in self-gravitating simulations. Our results validate the use of the outflow vBCs in SI simulations, even with vertical boundaries close (≤0.4H) to the disk midplane. Overall, our study demonstrates the numerical robustness of nonlinear particle clumping by the SI.

Rigor bonds cause reduced sarcomeric volume in skinned porcine skeletal muscle under PSE-like conditions

Effects of rigor cross-bridge attachment on water holding in PSE-like conditions were investigated. A new model using permeabilised/skinned porcine longissimus thoracis et lumborum muscle fibres was established to study effects of varied pH, temperature and the presence of rigor bonds on the muscle fibre structure. Preparations were exposed during 30min to different temperatures (22 and 38°C) and pH (7.0 and 5.5) in relaxed and rigor states, followed by evaluation of active force and filament lattice spacing (using small angle X-ray scattering) at standard pH (7.0) and temperature (22°C) conditions. A significant reduction of active force and filament spacing was observed after treatment in rigor at denaturing (38°C, pH5.5) conditions. The present study show that rigor bond attachment under denaturing conditions leads to a compressed interfilamental space, loss of sarcomeric water and affected contractile function.

Cargo navigation across 3D microtubule intersections

The eukaryotic cell's microtubule cytoskeleton is a complex 3D filament network. Microtubules cross at a wide variety of separation distances and angles. Prior studies in vivo and in vitro suggest that cargo transport is affected by intersection geometry. However, geometric complexity is not yet widely appreciated as a regulatory factor in its own right, and mechanisms that underlie this mode of regulation are not well understood. We have used our recently reported 3D microtubule manipulation system to build filament crossings de novo in a purified in vitro environment and used them to assay kinesin-1-driven model cargo navigation. We found that 3D microtubule network geometry indeed significantly influences cargo routing, and in particular that it is possible to bias a cargo to pass or switch just by changing either filament spacing or angle. Furthermore, we captured our experimental results in a model which accounts for full 3D geometry, stochastic motion of the cargo and associated motors, as well as motor force production and force-dependent behavior. We used a combination of experimental and theoretical analysis to establish the detailed mechanisms underlying cargo navigation at microtubule crossings.

Fibre Ultrastructure.

Mammalian hair fibres can be structurally divided into three main components: a cuticle, cortex and sometimes a medulla. The cuticle consists of a thin layer of overlapping cells on the surface of the fibre, constituting around 10% of the total fibre weight. The cortex makes up the remaining 86-90% and is made up of axially aligned spindle-shaped cells of which three major types have been recognised in wool: ortho, meso and para. Cortical cells are packed full of macrofibril bundles, which are a composite of aligned intermediate filaments embedded in an amorphous matrix. The spacing and three-dimensional arrangement of the intermediate filaments vary with cell type. The medulla consists of a continuous or discontinuous column of horizontal spaces in the centre of the cortex that becomes more prevalent as the fibre diameter increases.

Effect of Laser-arc Hybrid Welding Energy Parameters on Welding Stability

The high-speed camera was used to collect the droplet transfer pattern and arc pattern of the laser-arc hybrid welding process. Using the methods of image processing and mathematical statistics, the effects of different laser and arc power conditions on the welding stability were studied. The results show that the melting width depends on the welding current, the depth of penetration depends on the laser power, the droplet transition pattern, the actual filament spacing and the arc length determine the welding stability of the laser arc hybrid welding.

Observation of thick toroidal filaments during the disruptive phase of Aditya tokamak plasma

Major disruptions in Aditya tokamak are initiated by the growth and subsequent locking of m/n = 2/1 and 3/1 tearing modes, which leads to the thermal quench of the plasma. Thick filaments are seen to evolve at the low field side (LFS) of the plasma column following the thermal quench, and during the current quench. The number of filaments and inter filament spacing are observed to be related with the plasma stored energy just prior to the disruption. Rapid enhancement of the outward particle flux is seen during the thermal quench phase and the plasma conductivity reduces considerably. Interchange modes, with low poloidal wavenumber, are inferred to grow due to the reduced plasma conductivity and enhanced effective diffusivity. This may be a plausible explanation for the visualization of the thick filaments at the LFS.

Fabrication of Polydimethylsiloxane films with special surface wettability by 3D printing

Abstract Special wettability of biological surfaces usually depends on the unique micro-structures and surface chemistry natures. However, current preparation methods often involve complex processing steps or expensive moulds and have the limited ability to simulate these ordered and elaborate micro-structures. Herein, we report a facile and mould-free approach to fabricate special wetting surfaces by patterning an ordered porous structure with various geometric parameters via one-step 3D printing of Polydimethylsiloxane (PDMS) ink. By adjusting pore structure at sub-millimeter scale, the printed PDMS porous films can present versatile special surface wettabilities such as superhydrophobicity and anisotropic wettability. With the guide of computer program, water contact angles (WCAs) along parallel and perpendicular directionsand anisotropy wetting degree of the printed film can be tailored by controllingprinting speed and filament spacing. Through optimizing the geometrical parameters, a superhydrophobic PDMS filmwith a WCA of about 155° is obtained, which mainly depends on the sub-millimeter scale structure. The superhydrophobicity of the printed porous PDMS film also exhibits excellent durability against thermal aging. Our work demonstrates that the printed superhydrophobic porous film can be used for some attracting applications such as air-breathable water-proofing, water-repellent floating carrier, and no-loss liquid transfer.

Flightin maintains myofilament lattice organization required for optimal flight power and courtship song quality in Drosophila.

The indirect flight muscles (IFMs) of Drosophila and other insects with asynchronous flight muscles are characterized by a crystalline myofilament lattice structure. The high-order lattice regularity is considered an adaptation for enhanced power output, but supporting evidence for this claim is lacking. We show that IFMs from transgenic flies expressing flightin with a deletion of its poorly conserved N-terminal domain (flnΔN62 ) have reduced inter-thick filament spacing and a less regular lattice. This resulted in a decrease in flight ability by 33% and in skinned fibre oscillatory power output by 57%, but had no effect on wingbeat frequency or frequency of maximum power output, suggesting that the underlying actomyosin kinetics is not affected and that the flight impairment arises from deficits in force transmission. Moreover, we show that flnΔN62 males produced an abnormal courtship song characterized by a higher sine song frequency and a pulse song with longer pulses and longer inter-pulse intervals (IPIs), the latter implicated in male reproductive success. When presented with a choice, wild-type females chose control males over mutant males in 92% of the competition events. These results demonstrate that flightin N-terminal domain is required for optimal myofilament lattice regularity and IFM activity, enabling powered flight and courtship song production. As the courtship song is subject to female choice, we propose that the low amino acid sequence conservation of the N-terminal domain reflects its role in fine-tuning species-specific courtship songs.

Myosin MgADP Release Rate Decreases as Sarcomere Length Increases in Skinned Rat Soleus Muscle Fibers

Actin-myosin cross-bridges use chemical energy from MgATP hydrolysis to generate force and shortening in striated muscle. Previous studies show that increases in sarcomere length can reduce thick-to-thin filament spacing in skinned muscle fibers, thereby increasing force production at longer sarcomere lengths. However, it is unclear how changes in sarcomere length and lattice spacing affect cross-bridge kinetics at fundamental steps of the cross-bridge cycle, such as the MgADP release rate. We hypothesize that decreased lattice spacing, achieved through increased sarcomere length or osmotic compression of the fiber via dextran T-500, could slow MgADP release rate and increase cross-bridge attachment duration. To test this, we measured cross-bridge cycling and MgADP release rates in skinned soleus fibers using stochastic length-perturbation analysis at 2.5 and 2.0 μm sarcomere lengths as pCa and [MgATP] varied. In the absence of dextran, the force-pCa relationship showed greater Ca2+ sensitivity for 2.5 vs. 2.0 μm sarcomere length fibers (pCa50 = 5.68 ± 0.01 vs. 5.60 ± 0.01). When fibers were compressed with 4% dextran, the length-dependent increase in Ca2+ sensitivity of force was attenuated, though the Ca2+ sensitivity of the force-pCa relationship at both sarcomere lengths was greater with osmotic compression via 4% dextran compared to no osmotic compression. Without dextran, the cross-bridge detachment rate slowed by ∼15% as sarcomere length increased, due to a slower MgADP release rate (11.2 ± 0.5 vs. 13.5 ± 0.7 s−1). In the presence of dextran, cross-bridge detachment was ∼20% slower at 2.5 vs. 2.0 μm sarcomere length due to a slower MgADP release rate (10.1 ± 0.6 vs. 12.9 ± 0.5 s−1). However, osmotic compression of fibers at either 2.5 or 2.0 μm sarcomere length produced only slight (and statistically insignificant) slowing in the rate of MgADP release. These data suggest that skeletal muscle exhibits sarcomere-length-dependent changes in cross-bridge kinetics and MgADP release that are separate from, or complementary to, changes in lattice spacing.

Effect of barrier capacitance on self-organized structure in dielectric-barrier discharge microplasma

We experimentally observed variations in self-organized microgap barrier discharge with changes in barrier capacitance. We also performed a computer simulation using a reaction–diffusion equation. The simulation results showed the same tendency for the lattice spacing and size of filaments as hexagonal pattern structures in discharge experiments. We confirmed that the experimental result of the dependence of voltage on filament number density is consistent with the simulation result. From a theoretical viewpoint, it is possible that the size of filaments of a hexagonal structure caused by Turing instability corresponds to the characteristic wavelength obtained by simple analysis.