Fig Genotypes Research Articles

Development of Molecular Tools for Characterization and Genetic Diversity Analysis in Tunisian Fig (Ficus carica) Cultivars

Fig, Ficus carica L., is a useful genetic resource for commercial cultivation. In this study, RAPD (60), ISSR (48), RAMPO (63), and SSR (34) markers were compared to detect polymorphism and to establish genetic relationships among Tunisian fig tree cultivars. The statistical procedures conducted on the combined data show considerable genetic diversity, and the tested markers discriminated all fig genotypes studied. The identification key established on the basis of SSR permitted the unambiguous discrimination of cultivars and confirmed the reliability of SSR for fingerprinting fig genotypes. The study findings are discussed in relation to the establishment of a national reference collection that will aid in the conservation of Tunisian fig resources.

Evaluation of selected fig genotypes from Southeast Turkey

Turkey has great variations and distributions in wild fig forms as well as fig cultivars. This study was carried out in Mardin and
anlýurfa provinces during 2004 - 2005. Nine fig genotypes were evaluated in this selection study for two years. In this study, some morphological characteristics investigated were tree growth habit, tree vigour, relative degree of branching, leaf shape and leaf margin dentation. Also, the characteristics of all the fig genotypes were identificated. 63-07-006 and 63-07-007 fig genotypes which had the highest scores in all the fig genotypes according to analysis in the weighted ranked method were evaluated to be best table fig genotypes. In pomological characteristics, fruit weight ranged between 71.77 g (47-02-003) and 43.29 g (63-07-005). Some other values were: 65.26 mm (47-02-003) and 46.99 mm (47-02-019) of width; 56.87 mm (47-02-003) and 38.23 mm (47-02-008) of length; 4.44 mm (63-07-007) and 3.58 mm (47-02-020) of ostiole width; 28.57% (47-02-012) and 16.87% (63-07-007) of total soluble solids (TSS) ratio were determined.

RAPD-based study of genetic variation and relationships among wild fig genotypes in Turkey

The fig tree (Ficus carica L.) is of significant socio-economic importance in Turkey, with 25% of the world's fig production. Genetic variation and relationships among 14 wild-grown figs sampled from Coruh Valley in Turkey were characterized by random amplified polymorphic DNA (RAPD). Ninety-eight DNA fragments were scored after amplification of DNA samples with 13 random primers; 70% of the scored bands were polymorphic. Genetic distances between the fig genotypes ranged from 0.21 to 0.62. Genotypes 08-ART-02 and 08-ART-06 were found to be the most closely related, whereas 08-ART-09 and 08-ART-10 were the most distant. The 14 wild-grown genotypes were grouped into six main clusters and one outgroup. We conclude that RAPD analysis is efficient for genotyping wild-grown fig genotypes.

FIG GENETIC RESOURCES AND RESEARCH AT THE US NATIONAL CLONAL GERMPLASM REPOSITORY IN DAVIS, CALIFORNIA

The National Clonal Gcrmplasm Repository (NCGR) in Davis, California houses most of the Mediterranean-adapted fruit and nut crop collections in the US, including the fig. The NCGR is part of the United States Department of Agriculture (USDA) National Plant Germplasm System (NPGS). Our missions are to acquire, preserve, characterize and distribute germplasm resources of our designated crops. The NCGR fig collection currentl y includes 190 different accessions: 78 named fruiting cultivars, 44 regional selections from diverse locations, 40 advanced selections from plant breeders, 28 caprifigs, and a small number of species and hybrids. It is NPGS policy to distribute plant material, free of charge, to research interests around the world (see our website http://www.ars-grin.gov/dav/) . We have initiated DNA microsatellite fingerprinting of NCCR fig accessions, and anticipate complete testing of our collection over the next year. Proper identification is a key concern of the NCGR since individual fig cultivars have been widely distributed with man y s ynonyms, and often the same name used for different cultivars. To finalize identification, it will also be necessary to compare fingerprints to type material from other collections. The microsatellite information and AFL.P data will also make it possible to assess relatedness among fig genotypes, and will facilitate understanding of evolution within the genus Ficus. We are committed to acquiring additional material and are very interested in learning of opportunities, with a special interest in protecting collections which ma y otherwise be lost.

Molecular Characterization of Moroccan Fig Germplasm Using Intersimple Sequence Repeat and Simple Sequence Repeat Markers To Establish A Reference Collection

A study was conducted to identify genotypes present in a Moroccan fig germplasm collection and provide the first database for a reference collection in northern Morocco. In total, 75 fig samples were analyzed using 8 intersimple sequence repeat primers and 6 simple sequence repeat loci. From these samples, we identified 72 fig genotypes. In genetically heterogeneous cultivars, genotypes under the same denomination were distinguished by both molecular markers and pomological traits. Molecular analysis was used to classify the germplasm into 46 well-defined cultivars and 6 caprifig trees. The remaining genotypes were not clearly identified due to three cases of mislabeling and four cases of homonymy. No evidence was found for the occurrence of geographically widespread genotypes.