Objective – This research aimed to investigate potential disparities between genders in Croatia regarding their pro-environmental attitudes and mindsets, focusing on understanding how gender influences individuals' environmental consciousness and support for sustainable practices. This paper presents an empirical study utilizing survey data to examine gender differences in environmental attitudes and behaviors. Methodology – It contributes to the field of environmental sociology by exploring the role of gender in shaping individuals' responses to environmental challenges, with implications for policy development and sustainability initiatives. By employing an online survey via snowball sampling, a total of 263 adult participants from Croatia were recruited for the study. Closed-ended questions on a Likert scale measured respondents' attitudes towards recycling, waste sorting, environmental protection urgency, and health impacts of environmental issues. Findings – The findings in this research indicate that gender plays a role in influencing individuals' environmental attitudes and behaviors, with significant gender-based variations in environmental consciousness. Women exhibit higher levels of concern for environmental issues, stronger support for recycling policies, and a more positive outlook on the health implications of ecological problems. This study provides valuable insights into the connection between gender and environmentalism in Croatia, making significant contributions to existing literature. Moreover, it offers implications for the development of policies targeting environmental management and recognizing the need to influence the environmental behavior of each gender. By focusing on gender disparities in pro-environmental behaviors, the study offers new insights into the connection between gender and environmentalism, emphasizing the need for gender-sensitive approaches to environmental policymaking and intervention. Novelty – The research aims to deepen our understanding of the intersection between gender and environmental attitudes, highlighting the importance of incorporating gender-specific perspectives into efforts to promote sustainable behaviors, address environmental challenges, and foster more environmentally conscious societies in Croatia and beyond. Type of Paper: Empirical JEL Classification: F64, J16, Q54, Q56 Keywords: environmental attitudes, sustainability, eco-awareness, environmental behavior Reference to this paper should be made as follows: Mandarić, D; Hunjet, S.A. (2024). Gender Disparities in Pro-Environmental Attitudes: Implications for Sustainable Business Practices in Croatia, J. Bus. Econ. Review, 9(1), 73–84. https://doi.org/10.35609/jber.2024.9.1(4)