Pharmaceutical Field Research Articles

Green Biosynthesis of TiO2Nanoparticles Using Aqueous Extract ofBrassica juncea(L.) Czern Seed: Characterization, Antimicrobial, Antioxidant and Anticancer Activities

AbstractPrimary objective of this research was to examine the biogenic production of TiO2NPs usingBrassica juncea (L.)Czern seed and investigate the prospective uses of thus synthesized nanoparticles in the area of biomedicine. The synthesized TiO2NPs were subjected to characterization using several analytical methods namely TEM, UV‐vis, XRD, FE‐SEM, FTIR and EDAX. The average particle size (TEM) is found to be 38 nm, exhibiting irregular shapes. The FT‐IR spectra indicated that the extract contains functional groups, such as polyphenolic, hydroxyl and carboxylic groups, which are likely responsible for formation of nanoparticles. The cytotoxicity studies of the synthesized TiO2NPs against breast cancer cells (MCF‐7) and lung cells (A549) are shown to be dosage dependent. The inhibitory doses (IC50) were determined to be 43.98 μg/mL and 74.50 μg/mL on MCF‐7 and A549 cells, respectively. Green synthesized TiO2NPs were assessed for antioxidant activity inhibition rate (DPPH 89.42 %). TiO2NPs exhibited a maximal zone of inhibition of Staphylococcus aureus 23±0.20 mm against and 20±0.30 against Klebsiella pneumoniae at a concentration of 100 μg/mL, indicating strong antibacterial activity. The findings suggestBrassica juncea (L.)Czern seed encapsulated TiO2NPs possesses excellent biological applications which can be explored for its application in pharmaceutical field.

Nickel(II)‐Catalyzed Reductive Coupling of Xanthate Esters with Sulfur‐Containing and Selenium‐Containing Compounds: Synthesis of Unsymmetric Sulfides and Selenides

Comprehensive SummaryUnsymmetric sulfides and selenides have great applications in the pharmaceutical field. Herein, we describe the reductive coupling reaction of xanthate esters with sulfur‐containing and selenium‐containing compounds (thio(seleno)sulfonates and disulfides(selenides)) under the nickel‐catalyzed condition. It provides a mild and effective method for the synthesis of unsymmetric sulfides and selenides which has the advantages of mild reaction conditions, high yields and a wide range of substrates.

Đánh giá độc tính cấp và độc tính bán trường diễn của viên nang cứng Probiotic EntVN 500 mg trên động vật thực nghiệm

Probiotic EntVN 500 mg contains the probiotic strains E. faecium F26BA and E. faecium F54BA, both of which were isolated from traditional Vietnamese foods. This study's goal is to evaluate the safety of Probiotic EntVN 500 mg through an acute and semi-chronic toxicity oral administration. Toxicity studies on Wistar rats showed that even at a dose of 10 000 mg/kg body weight-40 times higher than the typical human dose-no 50% mortality rate (LD50) was observed. After administering EntVN Probiotic 500 mg to sewer rat at a daily dose of 140 mg/kg body weight (equivalent to the human dosage) and 420 mg/kg body weight (three times the human dosage) over a four-week period, no significant changes were observed hematological parameters including red blood cells, white blood cells, hemoglobin, hematocrit, and platelets. Blood chemistry tests, measuring levels of AST, ALT, cholesterol, albumin, and creatinine, also showed no significant variations. Additionally, histopathological examinations of the liver, spleen, and kidneys revealed no damage. Mice after using EntVN 500 mg increased their weight slightly compared to the control group. The concentration of viable probiotic bacteria was 3.3 x 1010 CFU/g at the time of production, and 3 x 1010 CFU/g one month later, indicating stable potency. The capsule formulation complies with Vietnam's national standards for bacterial contamination limits and heavy metal contamination limits. The results indicate that the capsule Probiotic EntVN 500 mg ingredients are safe and have potential applications in the field of pharmaceuticals and functional foods

Volumetric, compressibility and viscometric studies on intermolecular interactions of NaC and NaDC with Emtricitabine and Lamivudine in aqueous medium at (298.15–313.15) K

Bile salts are bio-compatible natural amphiphiles having a significant role in pharmaceutical field as a solubility and permeability enhancer and possess a great biological importance for release and transport of nutrients due to dynamic micellization behavior. Hence, to unfold the interactional mystery of drug − bile salt system in the field of anti-retroviral drugs, we have aimed to investigate solute–solute and solute–solvent interactions of bile salts (NaC and NaDC) with anti-HIV drugs (Emtricitabine and Lamivudine) by using various techniques like volumetry, compressibility, and viscometry at temperature range (298.15 K-313.15 K) with a regular variation of 5 K. Experimental density and speed of sound data have been used to evaluate various physicochemical parameters such as apparent molar volume (φv), isentropic compressibility (κs), and apparent molar adiabatic compression (φκ). The CMC values of bile salts in pure water and aqueous solution of ECT and LMV at 298.15 K have also been evaluated from speed of sound data which corroborate well with our earlier conductivity study on these surfactants. The trends of φv, κs and φκ values for both NaC and NaDC with temperature and in presence of drugs (ECT/LMV) indicate strong solute–solvent interactions in studied system. The results are elucidated in terms of electrostatic and hydrophilic-hydrophobic interactions in bile salt-drug-water system which is confirmed by co-sphere overlap model. Comparatively, NaC offers strong interactions with both drugs due to more hydrophilic character than NaDC. Similarly, out of ECT and LMV, ECT shows strong solute–solvent interactions with both bile salts due to more hydrophilic character than LMV. Relative viscosity parameter (ηr) determined from viscosity measurements further supports above results. This interactional knowledge of bile salts with ECT and LMV would be beneficial to develop a better mechanism in pharmacy for enhancing efficacy and permeability of anti-HIV drugs by using bile salts as a drug delivery agent.

Integrated exploration of molecular structure, quantum chemical properties, molecular docking, and antioxidant activity of 4-(2-hydroxyanilino)pent-3-en-2-one

Schiff bases are crucial intermediates in organic chemistry. They are used to introduce carbon–nitrogen double bonds in organic molecules. In this study, 4-(2-hydroxyanilino) pent-3-in-2-one (L) was synthesized from the reaction of 2,4-pentanedione and 2-aminophenol. The structure of L was confirmed through analytical and spectroscopic data. In addition, its thermal stability was examined using thermogravimetric and differential thermal analysis. In the gas phase, investigations of the highest occupied molecular orbital (HOMO) and lowest unoccupied molecular orbital (LUMO) revealed an energy gap (ΔE) of 3.9304 eV. The antioxidant capacity of L was assessed using various techniques, including hydroxyl radical scavenging, scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH), β-carotene bleaching, and scavenging of 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS). The synthesized Schiff base displayed significant antioxidant activity, with a half-maximal inhibitory concentration (IC50) of 19.8 µg/mL in the DPPH assay, 0.14 µg/mL in the ABTS assay, and 59.6 μg/mL in the hydroxyl radical scavenging assay. The bioactivity of the 4-(2-hydroxyanilino) pent-3-in-2-one was confirmed by the DFT study, and the MEP analysis indicates the reactivity of nitrogen and oxygen atoms. Docking studies and wave function analyses were conducted to provide further insights into the compound’s potential applications in the pharmaceutical field.

Wave-Assisted Techniques, a Greener and Quicker Alternative to Synthesis of Cyclodextrin-Based Nanosponges: A Review.

The ever-increasing applications of cyclodextrin and cyclodextrin-based nanosponges in formulation development has gained much attention from researchers towards needed research in this arena. Nanosponges are three-dimensional nanoporous versatile carriers in the pharmaceutical research field because of their capability to encapsulate lipophilic and hydrophilic drugs both in their crystalline structure by inclusion and non-inclusion phenomenon. This review sheds light on the advancements made in this field and the associated patents with regard to their synthesis while zooming in on the utilization of two novel energies (Microwave and ultrasonic) in accomplishing this goal and its future thereof. Microwave and ultrasound-assisted manufacturing of cyclodextrin-based nanosponges (CDNS) has been found superior to conventional heat-dependent methods due to rapid/homogenous heating and fast kinetics, which ultimately provide the final product with high yield and crystallinity relatively rapidly. The review article also defines several facets of microwave and ultrasound-assisted nanosponge synthesis including the synergism of microwave and ultrasonic energy and the theories behind them. This hitherto unexplored microwave-ultrasonic coupling technology could be a future technology to synthesize CD-NS with a better outcome. In the recent past, these novel energy processes have been used successfully in material synthesis at an industrial scale due to their swift and streamlined synthesis attributes. Likewise, these wave-assisted methods have the full potential to materialize the concept of CD-NS from lab scale to industrial scale as a competent and versatile drug carrier, having all the prerequisite characteristics, for commercialization.

Lyophilised protein formulations as a patient-centric dosage form: A contribution toward sustainability paradigm.

At present, society has embraced the fact apropos population aging and climate changes, that demand, amongst others, innovative pharmaceutical technologies, emphasising the development of patient-specific delivery systems and thus the provision of efficient and sustainable drugs. Protein drugs for subcutaneous administration, by allowing less frequent application, represent one of the most important parts of the pharmaceutical field, but their development is inevitably faced with obstacles in providing protein stability and suitable formulation viscosity. To gain further knowledge and fill the gaps in the already constructed data platform for the development of monoclonal antibody formulations, we designed a study that examines small model proteins, i.e., bovine serum albumin. The main aim of the presented work is to evaluate the effect of protein concentrations on critical quality attributes of both, pre-lyophilised liquid formulations, and lyophilised products. Through the study, the hypothesis that increasing protein concentration leads to higher viscosity and higher reconstitution time without affecting the stability of the protein was confirmed. The most important finding is that sucrose plays a key role in the lyophilisation of investigated protein, nevertheless, it can be predicted that, to ensure the beneficial effect of mannitol, its amount has to prevail over the amount of sucrose.

Black soldier fly (Hermetia illucens L.): A potential small mighty giant in the field of cosmeceuticals.

Natural products are widely used in the pharmaceutical and cosmetics industries due to their high-value bioactive compounds, which make for "greener" and more environmentally friendly ingredients. These natural compounds are also considered a safer alternative to antibiotics, which may result in antibiotic resistance as well as unfavorable side effects. The development of cosmeceuticals, which combine the cosmetic and pharmaceutical fields to create skincare products with therapeutic value, has increased the demand for unique natural resources. The objective of this review is to discuss the biological properties of extracts derived from larvae of the black soldier fly (BSF; Hermetia illucens), the appropriate extraction methods, and the potential of this insect as a novel active ingredient in the formulation of new cosmeceutical products. This review also addresses the biological actions of compounds originating from the BSF, and the possible association between the diets of BSF larvae and their subsequent bioactive composition. A literature search was conducted using PubMed and Google Scholar to identify and evaluate the various biological properties of the BSF. One such natural resource that may be useful in the cosmeceutical field is the BSF, a versatile insect with numerous potential applications due to its nutrient content and scavenging behavior. Previous research has also shown that the BSF has several biological properties, including antimicrobial, antioxidant, anti-inflammatory, and wound healing effects. Given the range of biological activities and metabolites possessed by the BSF, this insect may have the cosmeceutical potential to treat a number of skin pathologies.

Characterization of titanium dioxide nanomaterials synthesized from leaf extract of Pogostemon cablin Benth and their potential antifungal activity

Recently, dermal infections caused by fungus have increased drastically, affecting people’s daily lives and causing embarrassment. These infections are either due to dermatophytes or non-dermatophytic fungi. Basically, three genera of dermatophytes namely Trichophyton, Microsporum and Epidermohyton are the main cause of dermatophytosis. Candida albicans is also responsible for some dermal infections as a non-dermatophytic fungus. Traditional antifungal compounds cause side effects and resistance in fungi, so better and non-toxic alternatives are needed. Now a day’s, nanotechnology is widely used in the pharmaceutical field and has proven to be a good antifungal agent. This study used TiO2 nanoparticles (NPs) against four human pathogens Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum gypseum, and Candida albicans. Green and chemical synthesis methods were used for the synthesis of TiO2 nanoparticles. In green synthesis, we used aqueous leaf extract of Pogostemon cablin Benth. The formation of TiO2 nanoparticles is confirmed by UV-visible spectroscopy, Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Fourier Transform Infrared (FTIR) and X-ray Diffraction (XRD) analysis. According to XRD, the average size of chemically synthesized nanoparticles was 70.52 nm, while green synthesized nanoparticles were 71.82 nm. All the characterizations support that Pogostemon cablin aqueous extract can synthesize TiO2 nanoparticles. The minimum inhibitory concentration was determined according to the broth microdilution recommended assay. This study concluded that green synthesized TiO2 NPs showed good results against all four pathogens.

A review: Pharmacological and phytochemical update of Passiflora edulis F. Flavicarpa

A native of tropical America (Brazil), passion fruit (Passiflora edulis F. Flavicarpa) is a desirable, high-value crop that is a member of the Passifloraceae family. The fruits of the yellow species (Passiflora edulis F. Flavicarpa) are almost round to oval in shape, with a stiff peel that is smooth and waxy, and weigh approximately 35-40 grams. Plants are used medicinally all over the world for their many health advantages; this particular plant has the highest concentration of phytoconstituents. Numerous phytoconstituents, including flavonoids, tannins, phenols, glycosides, fatty acids, and alkaloids, are present, according to the review. According to reports, Passiflora edulis F. Flavicarpa has anti-inflammatory, anti-convulsant, anti-microbial, anti- cancer, anti-diabetic, anti-hypertensive, anti-sedative, and anti-oxidant qualities. It can also be used as a colon cleanser and have a variety of therapeutic uses for ailments like osteoarthritis and asthma. The various plant parts have also been used as sedatives, sleep aids, ulcer and hemorrhoid treatments, and digestive aids. The ethnopharmacology, phytoconstituents, and medicinal advantages of plants are briefly reviewed in the article, emphasizing the need for more study and advancement in the pharmaceutical field.

Counter-intuitive discovery in the formulation of poorly water-soluble drugs: Amorphous small-molecule gels.

Amorphous strategies have been extensively used in improving the dissolution of insoluble drugs for decades due to their high free energy. However, the formation of amorphous small-molecule gels (ASMGs) presents a counter-intuitive discovery that significantly limits their practical application. Recently, ASMGs have garnered attention because of their noncovalent structures, excellent biodegradability, and significant potential in various drug delivery systems in the pharmaceutical field. Hence, a comprehensive review is necessary to contribute to a better understanding of recent advances in ASMGs. This review aimed to introduce the main formation mechanisms, summarize possible influencing factors, generalize unique properties, outline elimination strategies, and discuss clinical application potential with preclinical cases of ASMGs. Moreover, few ASMGs are advanced to clinical stages. Intensive clinical research is needed for further development. We hope that this review can provide more efficient and rational guidance for exploring further clinical applications of ASMGs.

Expedient Synthesis of Alkyl and Aryl Thioethers Using Xanthates as Thiol-Free Reagents.

Thioethers are critical in the fields of pharmaceuticals and organic synthesis, but most of the methods for synthesis alkyl thioethers employ foul-smelling thiols as starting materials or generate them as by-products. Additionally, most thiols are air-sensitive and are easily oxidized to produce disulfides under atmospheric conditions; thus, a novel method for synthesizing thioethers is necessary. This paper reports a simple, effective, green method for synthesizing dialkyl or alkyl aryl thioether derivatives using odorless, stable, low-cost ROCS2K as a thiol surrogate. This transformation offers a broad substrate scope and good functional group tolerance with excellent selectivity. The reaction likely proceeds via xanthate intermediates, which can be readily generated via the nucleophilic substitution of alkyl halides or aryl halides with ROCS2K under transition-metal-free and base-free conditions.

Fluoroalkoxylating Reagents in Organic Synthesis: Recent Advances

AbstractThe fluoroalkoxy groups (ORf) are widely used motifs in pharmaceutical and agrochemical fields due to their unique physicochemical properties including higher lipophilicity and increased metabolic stability. Thus, the high value of ORf‐containing derivatives has stimulated the development of fluoroalkoxylation reactions. In this review, we highlight the recent progress of various trifluoroalkoxylating reagents and their applications in organic synthesis in the past five years.

Progress in the Preparation and Application of Inulin-Based Hydrogels.

Inulin, a natural polysaccharide, has emerged as a promising precursor for the preparation of hydrogels due to its biocompatibility, biodegradability, and structural versatility. This review provides a comprehensive overview of the recent progress in the preparation, characterization, and diverse applications of inulin-based hydrogels. Different synthesis strategies, including physical methods (thermal induction and non-thermal induction), chemical methods (free-radical polymerization and chemical crosslinking), and enzymatic approaches, are discussed in detail. The unique properties of inulin-based hydrogels, such as stimuli-responsiveness, antibacterial activity, and their potential as fat replacers, are highlighted. Special emphasis is given to their promising applications in drug delivery systems, especially for colon-targeted delivery, due to the selective degradation of inulin via colonic microflora. The ability to incorporate both hydrophilic and hydrophobic drugs further expands their therapeutic potential. In addition, the applications of inulin-based hydrogels in responsive materials, the food industry, wound dressings, and tissue engineering are discussed. While significant progress has been achieved, challenges and prospects in optimizing synthesis, improving mechanical properties, and exploring new functionalities are discussed. Overall, this review highlights the remarkable properties of inulin-based hydrogels as a promising class of biomaterials with immense potential in the biomedical, pharmaceutical, and materials science fields.

Biosurfactant production by Bacillus amyloliquefaciens, characterization and its potential applications

Aim: To identify indigenous bacteria responsible for producing biosurfactants in soil contaminated by oil and to investigate their potential uses in the fields of pharmaceuticals and environmental applications. Methodology: The microorganisms of oil-contaminated soil were isolated, screened, and identified using 16S rRNA gene sequencing. Bacillus amyloliquefaciens was utilized to produce biosurfactants, and were characterized by UV, TLC, FTIR, HPTLC, and GCMS analysis. Antibacterial activity, plant growth promotion, biocompatibility, and wound healing activity of surfactant were performed. Results: Six bacterial cultures (B1 to B6) were isolated from the soil samples collected from various locations in Kerala and Tamil Nadu. Among the isolates screened for biosurfactant production, Bacillus amyloliquefaciens exhibited the highest capacity for biosurfactant production, confirmed through 16S rRNA gene sequencing. The biosurfactant produced was identified as surfactin of lipopeptide nature, characterized by various analytical techniques: UV absorption at 400 nm, TLC and HPTLC (Rf value of 0.58), FTIR (prominent peak at 3819 cm-1 corresponding to -OH groups), GCMS with a maximum retention time of 12.03. Surfactin effectively inhibited pathogenic bacteria, enhanced plant growth, was biocompatible, and enhanced the rate of cell migration towards wound scratch. Interpretation: The current study revealed that Bacillus amyloliquefaciens, isolated from oil-contaminated soil, is capable of producing biosurfactant surfactin. Additionally, it unveiled a broad spectrum of applications for surfactin, encompassing wound healing, biocompatibility, antimicrobial activity, and promotion of plant growth. Key words: Antibacterial activity, Biocompatibility, Bacillus amyloliquefaciens, Biosurfactant, Wound healing

Bioactive Compounds Produced by Macromycetes for Application in the Pharmaceutical Sector: Patents and Products

It is widely known that mushrooms present several properties with applications in the medicinal and pharmaceutical sectors, including antimicrobial, immunomodulatory, antioxidant, hypotensive, neuroprotective, and anti-inflammatory activities. This article aims to review examples of the bioactive metabolites responsible for those activities, such as polysaccharides, phenols and polyphenols, terpenes, peptides, alkaloids, and steroids, which are produced by several relevant mushroom species. It also discusses their production through solid-state fermentation and submerged fermentation, as well as the processes of obtention of mushroom bioactive extracts and considerations on their stability aiming industrial applications. In addition, the article examines the patent landscape surrounding mushroom-derived bioactives, shedding light on the intellectual property history and innovations driving this field forward. Examples of recently deposited patents in the field are highlighted, as well as the main depositors. China and the United States are the major depositor countries in this field (52% and 35% of patents, respectively), and the principal compounds on the patents are polysaccharides and alkaloids. The article also provides insights into the current market landscape, showcasing mushroom-derived products in the pharmaceutical field available to consumers. From dietary supplements to skincare formulations, the market offerings reflect the growing interest in harnessing the health benefits of mushroom bioactives.

Future of Pharmaceutical Industry: Role of Artificial Intelligence, Automation and Robotics

Background The future of smart factories and pharmaceutical industries has evolved significantly since the 19th century. Computers have been used in the pharmaceutical field since the 1980s with the emergence of Artificial Intelligence (AI). In addition, automation and robotics are used in the pharmaceutical industry to improve the efficiency of pharmaceutical development and production. Purpose The present review article covers the future roles of AI, automation and robotics in pharmaceutical industries. Methodology The current review article employed a comprehensive search strategy across relevant databases, including PubMed, Scopus and Web of Science, utilizing keywords such as AI, Automation, Robotics and Pharmaceutical Industries. The articles considered a focus on recent advancements and emerging trends in the intersection of AI, automation and robotics within pharmaceutical sectors. Results AI, incorporating predictive machine learning and reasoning techniques, aids in the preclinical identification of molecules and forecasting potential lead compounds before conducting clinical trials. automation offers significant benefits in monitoring and predictive maintenance of production lines, power distribution and control machines. Robotic process automation improves efficiency by connecting computer terminals to handle various manufacturing process elements. Conclusion Artificial intelligence, automation and Robotics have sparked innovations in the healthcare business, benefiting the global ecosystem and healthcare delivery. Incorporating these advanced tools in pharmaceutical industries from raw material selection to final product development could improve the quality and safety of the pharmaceutical products and reduce the time and cost.

Formulation of Ascorbic Acid and Betaine-based Therapeutic Deep Eutectic System for Enhanced Transdermal Delivery of Ascorbic Acid.

L-ascorbic acid (AA), a potent antioxidant, is commonly used topically in the pharmaceutical and cosmetic fields. However, the incorporation of AA into topical formulations is difficult because of its highly unstable nature and relatively poor skin permeability. In this study, we propose an alternative strategy for improving the solubility and topical delivery of AA through its conversion to a therapeutic deep eutectic system (THEDES). AA and betaine (Bet)-based THEDESs were prepared at certain molar ratios and characterized using polarized optical microscopy, Fourier transform infrared spectroscopy, and differential scanning calorimetry. Solubility tests showed that AA in the form of THEDES was readily soluble in various polyols (glycerin, 1,3-butylene glycol, dipropylene glycol, and 1,3-propanediol) at a high concentration (approximately 40%). Furthermore, compared to AA alone or the physical mixture of AA and Bet, AA-based THEDES significantly enhanced AA delivery through porcine skin. In an in vivo human study, THEDES-containing serum reduced the markers of aging and induced an even skin tone. These findings indicate the utility of AA and Bet-based THEDES as novel transdermal delivery systems for AA. Furthermore, our approach also showed good extension to developing gluconolactone, a well-known natural antioxidant, and Bet-based THEDES, showing potential application in transdermal delivery systems.

Continuous synthesis of drug particles via spray drying of emulsions obtained by mechanical emulsification and ouzo effect

In the pharmaceutical field, nanoparticle formulation is an effective approach to improve the solubility of poorly water-soluble drugs. Spray drying is used for the synthesis of fine drug particles. The particle size obtained by spray drying generally depends on the size of the sprayed droplet. In this study, an emulsion spray drying method is proposed as a novel method for nanoparticle synthesis. Oil-in-water (O/W) nanoemulsions containing oil-soluble drugs were prepared. Spraying O/W emulsions could produce fine particles with the size of 122 nm. The particles obtained by emulsion spray drying were smaller than those obtained by spray drying without an emulsion. Furthermore, an Ouzo emulsion, which is a self-emulsification method, was investigated to improve the particle yield. The particles obtained by spray drying of the Ouzo emulsion had the size of 0.50 μm, which was smaller than those obtained without the Ouzo emulsion. Moreover, in-vitro dissolution tests were performed to evaluate the solubility of the obtained particles as well as the unprocessed drug powder. The particles obtained via spray drying of the Ouzo emulsions showed the highest dissolution rate. In summary, spray drying of O/W and Ouzo emulsions resulted in the production of fine drug particles.

Preparation of silver nitroprusside nanoparticles for enhanced acid red 94 removal from effluent: A study on synergistic adsorption and photocatalytic processes, with toxicological assessment

Toxic dye pollution in water is an urgent global environmental issue. The synergistic action of adsorption and degradation has shown great removal effects for treating toxic dyes in aqueous environments. Silver ions and nitroprusside salts exhibit excellent antimicrobial, anticancer, and biocompatible properties and are widely used in the pharmaceutical field; however, their application in environmental treatment has not been extensively explored. Hence, we prepared novel and highly efficient silver nitroprusside nanoparticles (Ag2[Fe(CN)5NO] NPs) through a one-pot reaction using sodium nitroprusside and silver nitrate for the first removal of acid red dye (AR 94). Furthermore, Ag2[Fe(CN)5NO] NPs were characterized using various analytical techniques. The Ag2[Fe(CN)5NO] NPs have both cubic and rod-like structures with a size of 107.46 ± 34.54 d.nm, the PDI is 0.263 ± 0.018 and the zeta potential is −46.9 ± 0.026 mV. In addition, the synergistic adsorption and degradation performance of Ag2[Fe(CN)5NO] NPs was evaluated. The removal efficiencies of Ag2[Fe(CN)5NO] NPs on AR 94 can reach more than 95 % under natural light irradiation and optimal reaction conditions of 60 mg/L AR 94 dye concentration, pH 6, temperature 45 °C, and time 6 h. After 5 cycles of recycling, the removal efficiency remained above 90 %. The aqueous solution obtained after removing AR 94 demonstrated no adverse effects on Zea mays L. seed growth. It also exhibited low toxicity to Artemia salina. These results emphasize the potential of Ag2[Fe(CN)5NO] NPs as an efficient and environmentally friendly material for the removal of dyes from aqueous environments.