Field Strength Research Articles

Pulsed Electric Field for Quick-Cooking Rice: Impacts on Cooking Quality, Physicochemical Properties, and In Vitro Digestion Kinetics

Pulsed electric field (PEF) is one of the emerging technologies that has been applied in many aspects of the food industry. This study examined the impacts of a PEF on the cooking quality, physicochemical properties, nutritional factors, and in vitro protein and starch digestion of two varieties of rice, including Jasmine 105 (white non-glutinous rice) and San Pa Tong 1 (white glutinous rice). Response surface methodology (RSM) and a three-level, three-factor Box–Behnken design were employed to assess the effects of the pulse number, electric field strength, and frequency on cooking time. The findings demonstrated that the number of pulses was a crucial factor influencing cooking time. Under optimal conditions (3347–4345 pulses, electric field strengths of 6–8 kV/cm, and frequencies ranging from 6 to 15 Hz), the rice cooking time was significantly reduced by 40–50% (p < 0.05) when compared to a conventional method. Moreover, PEF-treated rice showed a significant enhancement in in vitro protein and starch digestibility (p < 0.05), as well as retained a higher content of rapidly digestible starch. These results suggested that PEF treatment is a promising green technology for producing a novel quick-cooking rice with an improved eating quality.

Quantification of breast biopsy clip marker artifact on routine breast MRI sequences: a phantom study.

To investigate the artifact sizes of four common breast clip-markers on a standard breast magnetic resonance imaging (MRI) protocol in an in vitro phantom model. Using 1.5-T and 3-T whole-body scanners with an 18-channel breast coil, artifact dimensions of four breast biopsy markers in an agarose-gel phantom were measured by two readers on images obtained with the following sequences: T2-weighted fast spin-echo short inversion time fat-suppressed inversion-recovery with magnitude reconstruction (T2-TIRM); T1-weighted spoiled gradient-echo with fat suppression (T1_FL3D), routinely used for dynamic contrast-enhanced imaging; diffusion-weighted imaging (DWI), including a readout segmented echo-planar imaging (RESOLVE-DWI) and echo-planar imaging sequence (EPI-DWI). After outlining the artifacts by freehand regions of interest, sagittal and lateral diameters in axial images were measured. Interreader agreement for artifact size quantification was high, depending on the sequence (80.4-94.8%). Overall, the size, shape, and appearance of artifacts depended on clip type and MRI sequence. The artifact size ranged from 5.7 × 8.5 mm2 to 13.4 × 17.7 mm2 at 1.5 T and from 6.6 × 8.2 mm2 to 17.7 × 20.7 mm2 at 3 T. Clip artifacts were largest on EPI-DWI and RESOLVE-DWI (p ≤ 0.016). In three out of four clips, T2-TIRM showed the smallest artifact (p ≤ 0.002), while in one clip the artifact was smallest on T1_FL3D (p = 0.026). With the exception of one clip in the RESOLVE sequence, all clips showed a decrease in the artifact area from DWI to ADC images (p ≤ 0.037). Breast clip-marker MRI artifact appearances depend on clip type, field strength, and sequence and may reach a significant size, potentially obscuring smaller lesions and hindering accurate assessment of breast tumors. Considerable variations in artifact size and characteristics across different breast clips, MRI sequences, and field strengths exist. Awareness of these artifacts and their characteristics is essential to ensure accurate interpretation of scans and appropriate treatment planning. Awareness of breast clip artifacts is essential for accurate interpretation of MRI. The appearance of artifacts depends on breast clip type, field strength, and sequence. Clip-related artifacts might hinder the visibility of small lesions.

Research on Typical Decay-like Fracture Defects of Composite Insulators Based on Electro-Thermal Coupling

In response to the typical decay and fracture defects of composite insulators, a three-dimensional electrically and thermally coupled simulation physical model was constructed based on the finite element method, and the local electric field distortion and temperature rise were analyzed. The study confirms that the insulator interface’s axial electric and thermal fields show a U-shaped curve; the interface field strength is the largest. There is an electric field gradient difference between the mandrel and the sheath, and the thermal field is concentrated at the mandrel and the interface. The field strength at the edge of the defect is the largest, the aberrant electric field at the defect shows a sawtooth shape, and the temperature rise is concentrated in the defect area. The degradation is fast in the air gap, the etching hole diameter direction, and the carbonation channel axial direction. The larger the defect volume, the larger the aberration in the electric field and temperature rise. Water vapor air gaps, breakdown holes, and carbonized channels have the most pronounced electric field and temperature changes. The functional relationship between electric field aberration, temperature rise, and defect volume is established. The results provide a basis for the protection of insulator decay-like fracture.

Effects of Al Substitution for Fe in Na₅FeSi₄O₁₂ (5.1.8) Glasses: Structure and Crystallization

In this study, the effects of substituting Al for Fe in 5Na2O∙(Al2O3)x∙(Fe2O3)1-x∙8SiO2 glass, x=0 to 1, and Na5AlxFe1-xSi4O12 (5.1.8) crystal, were investigated using thermal analysis, Fe K-edge X-ray absorption, X-ray diffraction, Raman spectroscopy, and Electron Probe Microanalysis. In both glass and crystallized glass, nearly all the Fe was tetrahedrally coordinated Fe3+, as expected from the high concentration of Na2O. The substitution of Al for Fe in the glasses caused the glass transition temperature to increase as polymerization increased, as evidenced by Raman, likely due to both field strength differences of Al vs Fe and a small amount of Fe2+ network modifier present with Fe. After heat treatment at 700 °C for 24 hours, the glasses had crystallized, forming Na2SiO3 and NaAlSiO4 in compositions with high Al concentrations and the 5.1.8 crystal in compositions with high Fe concentrations. Through electron microprobe, it was determined that <0.04 formula unit Al incorporated into the 5.1.8 crystal, i.e. Na5Fe0.96Al0.04Si4O12. The 5.1.8 crystal only formed when Fe concentration was higher than Al in the starting glass.

Short-duration gamma-ray bursts from Kerr–Newman black hole mergers

AbstractBlack hole (BH) mergers are natural sources of gravitational waves (GWs) and are possibly associated with electromagnetic events. Such events from a charged rotating BH with an accretion on to it could be more energetic and ultra-short-lived if the magnetic force dominates the accretion process because the attraction of ionized fluid with a strong magnetic field around the rotating BH further amplifies the acceleration of the charged particle via a gyromagnetic effect. Thus a stronger magnetic field and gravitational pull will provide an inward force to any fluid displaced in the radial direction and move it toward the axis of rotation with an increasing velocity. After many twists during rotation and the existence of restoring agents, Such events could produce a narrow intense jet starts in the form of Poynting flux along the axis of rotation resembling the Blandford–Znajek (BZ) mechanism. We investigated a charged rotating BH and obtained characteristic results (e.g., the remnant mass, magnetic field strength, luminosity, opening angle, viewing angle, and variation of viewing angle on the SGRB luminosity detection) that have a nice coincidence with rare events having GW associated with EM counterparts. This study gives a new insight into events with a strongly magnetized disk dominating the accretion process of energy extraction.

UGMRT Survey of EXoplanets Around M-dwarfs (GS-EXAM): Radio Observations of GJ 1151

Coherent radio emission with properties similar to planetary auroral signals has been reported from GJ 1151, a quiescent, slow-rotating mid-M star, by the LOFAR Two-meter (120–170 MHz) Sky Survey. The observed LOFAR emission is fairly bright at 0.89 mJy with 64% circular polarization, and the emission characteristics are consistent with the interaction between an Earth-sized planet with an orbital period of 1–5 days and the magnetic field of the host star. However, no short-period planet has been detected around GJ 1151. To confirm the reported radio emission caused by the putative planet around GJ 1151 and to investigate the nature of this emission, we carried out upgraded Giant Metrewave Radio Telescope observations of GJ 1151 at 150, 218, and 400 MHz over 33 hr across ten epochs. No emission was detected at any frequency. While at 150 and 218 MHz, nondetection could be due to the low sensitivity of our observations, at 400 MHz, the rms sensitivities achieved were sufficient to detect the emission observed with LOFAR at ∼20σ level. Our findings suggest that the radio emission is highly time variable, likely influenced by the star-planet system’s phase and the host star’s magnetic field. Additional observations below 170 MHz, at more frequent epochs (as the periodicity of the emission is unknown), especially during periods of high stellar magnetic field strength, are needed to confirm the emission.

Large Scale Ultrafast Manufacturing of Wireless Soft Bioelectronics Enabled by Autonomous Robot Arm Printing Assisted by a Computer Vision-Enabled Guidance System for Personalized Wound Healing.

A Customized wound patch for Advanced tissue Regeneration with Electric field (CARE), featuring an autonomous robot arm printing system guided by a computer vision-enabled guidance system for fast image recognition is introduced. CARE addresses the growing demand for flexible, stretchable, and wireless adhesive bioelectronics tailored for electrotherapy, which is suitable for rapid adaptation to individual patients and practical implementation in a comfortable design. The visual guidance system integrating a 6-axis robot arm enables scans from multiple angles to provide a 3D map of complex and curved wounds. The size of electrodes and the geometries of power-receiving coil are essential components of the CARE and are determined by a MATLAB simulation, ensuring efficient wireless power transfer. Three heterogeneous inks possessing different rheological behaviors can be extruded and printed sequentially on the flexible substrates, supporting fast manufacturing of large customized bioelectronic patches. CARE can stimulate wounds up to 10 mm in depth with an electric field strength of 88.8 mVmm-1. In vitro studies reveal the ability to accelerate cell migration by a factor of 1.6 and 1.9 for human dermal fibroblasts and human umbilical vein endothelial cells, respectively. This study highlights the potential of CARE as a clinical wound therapy method to accelerate healing.

Spectra of solar shallow-water waves from bright point observations

Rossby waves, large-scale meandering patterns drifting in longitude, detected in the Sun, were recently shown to a play a crucial role in understanding “seasons” of space weather. Unlike Earth's purely classical atmospheric Rossby waves, the solar counterparts are strongly magnetized and most likely originate in the tachocline. Because of their deeper origin, detecting these magnetized Rossby waves is a challenging task that relies on careful observations of long-lived longitudinally drifting magnetic patterns at the surface and above. Here, we have utilized 3 years of global, synchronous observations of coronal bright point densities to obtain empirical signatures of dispersion relations that can be attributed to the simulated waves in the tachocline. By tracking the bright point densities at selected latitudes, we computed their wave-numbertimes frequency spectra. Wave-numbertimes frequency spectra were computed utilizing the Wheeler-Kiladis method. This method has been extensively used in the identification of equatorial waves in Earth's atmosphere by highlighting spectral peaks in the wave-numbertimes frequency space. Our results are compatible with the predictions of magneto-Rossby waves with typical periods of several months and inertio-gravity waves with typical periods of a few weeks, depending on the background magnetic field's strength and stratification at the convection zone base. Our analysis suggests that magnetized Rossby waves originate from the tachocline toroidal field of lesssim 15 kG. Global observations of bright points over extended periods will allow us to better constrain the stratification and magnetic field strength in the tachocline.

Stable Millivolt Range Resistive Switching in Percolating Molybdenum Nanoparticle Networks.

To overcome the limitations of the conventional Von Neumann architecture, inspiration from the mammalian brain has led to the development of nanoscale neuromorphic networks. In the present research, molybdenum nanoparticles (NPs), which were produced by means of gas phase condensation based on magnetron sputtering, are shown to be the constituents of electrically percolating networks that exhibit stable, complex, neuron-like spiking behavior at low potentials in the millivolt range, satisfying well the requirement of low energy consumption. Characterization of the NPs using both scanning electron microscopy and scanning transmission electron microscopy revealed not only pristine shape, size, and density control of Mo NPs but also a preliminary proof of the working mechanism behind the spiking behavior due to filament formations. Furthermore, electrostatics COMSOL Multiphysics simulations of the morphology of the NPs provided evidence that the stable switching is due to the as-deposited stellate Mo NPs creating high electric field strengths, while keeping them separated, not seen before in other percolating networks based on spherical NPs. Hence, our results show the working mechanism behind switching in percolating Mo NP networks and show that they are very promising for realistic neuromorphic systems.

Bifunctional Luminescent Thermometer-Manometer Based on Cr3+-Cr3+ Pair Emission.

Pressure dependence of spectral positions of the 2E → 4A2 and 4T2 → 4A2 bands of Cr3+ ions, resulting from changes in Cr3+-O2- covalency and variations in crystal field strength, respectively, is commonly utilized in luminescence manometry. However, as demonstrated in this paper, the luminescence of Cr3+-Cr3+ pairs shows insensitivity to pressure changes, making this signal suitable as a luminescence reference. The significant difference in the thermal and pressure dependences of luminescence with Cr3+ occupying different crystallographic positions in CaAl12O19:Cr3+ enables the development of a dual-function luminescence thermometer that operates in both ratiometric and lifetime-based readout modes. The presented first demonstration on utilization of Cr3+-Cr3+ pairs luminescence for sensing applications may rekindle interest in Cr3+-Cr3+ pairs for the development of optical sensors for physical quantities.

Discrete Bio-Hazard Model of EM Radiation for Crowded People

This study investigates the behaviour of electromagnetic (EM) waves in densely populated environments using a Multicomponent Discrete Propagation Model (MCDM). Our analysis focuses on four critical parameters: Specific Absorption Rate (SAR), Electrical Field, Skin Depth, and Effective Radiated Power (ERP) by working on crowd densities and frequencies relevant to mobile technologies. This study is the first to employ a Propagation Model for such an analysis, offering a novel approach. Through comprehensive simulations, we explore how variations in electrical field strengths impact SAR, skin depth, and ERP. The results are compared to established safety limits for whole-body SAR exposure, providing valuable insights for guidelines. This research aims to contribute to designing future electronic devices that minimize overall RF emissions. This innovative approach has the potential to improve public confidence in the safety of wireless technologies significantly

Effect of Na atom adsorption on the electronic structure of F-doped modified MoTe2 systems in the presence of an electric field: first principles

Abstract The effect of electronic structure and charge transfer on the adsorption of alkali metal sodium atoms by halogen-fluorine atom doped molybdenum ditelluride (MoTe2) has been investigated using a first-principles approach. It was found that the molybdenum ditelluride system underwent a direct bandgap semiconductor-to-metal transition after doping with halogen fluorine atoms. Upon adsorption of alkali metal Na atoms, the conduction band of the F-MoTe2 system shifts from metal to direct bandgap semiconductor. This semiconductor-to-metal-to-semiconductor bandgap modulation method can be well applied to photovoltaics. In addition, we discuss three potential adsorption sites: the hollow site (H), the bridge site (B) and the top site (T). The results showed that all three adsorption sites could be stabilized for adsorption. Subsequently, we selected the most stable B site and applied an electric field ranging from −0.5 eV Å−1 to 0.5 eV Å−1 to the system. At an electric field strength of −0.5 eV Å−1, the system transforms from a direct bandgap semiconductor to a metal. In terms of density of states, F-s, F-p, Te-s, Te-p, and Mo-d pass the Fermi energy level, increasing carrier concentration. It is hoped that these studies will play an important role in improving the photoelectric conversion efficiency.

Schwinger vs Coleman: Magnetic charge renormalization

Abstract The kinetic mixing of two U(1) gauge theories can result in a massless photon that has perturbative couplings to both electric and magnetic charges. This framework can be used to perturbatively calculate in a quantum field theory with both kinds of charge. Here we reexamine the running of the magnetic charge, where the calculations of Schwinger and Coleman sharply disagree. We calculate the running of both electric and magnetic couplings and show that the disagreement between Schwinger and Coleman is due to an incomplete summation of topological terms in the perturbation series. We present a momentum space prescription for calculating the loop corrections in which the topological terms can be systematically separated for resummation. Somewhat in the spirit of modern amplitude methods we avoid using a vector potential and use the field strength itself, thereby trading gauge redundancy for the geometric redundancy of Stokes surfaces. The resulting running of the couplings demonstrates that Dirac charge quantization is independent of renormalization scale, as Coleman predicted. As a simple application we also bound the parameter space of magnetically charged states through the experimental measurement of the running of electromagnetic coupling.

QCD with 2+1 flavors at the physical point in external chromomagnetic fields

We investigate full quantum chromodynamics (QCD) with (2+1)-flavor of highly improved staggered quarks fermions at the physical point in the presence of uniform Abelian chromomagnetic background fields. Our focus is to conduct an exploratory study on the renormalized light and strange chiral condensates around the pseudocritical temperature. We find that in the confined region the gauge system is subjected to the chromomagnetic catalysis that turns into the inverse catalysis in the high-temperature regime. We further observe that the chiral condensates are subjected to the so-called thermal hysteresis. Our estimate of the deconfinement temperature indicates that the critical temperature begins to decrease in the small field region, soon after it seems to saturate and finally increase with the strength of the chromomagnetic field. Published by the American Physical Society 2024

Membrane piezoelectric MDS-actuator with a flat double spiral of interacting electrodes

A schematic diagram and mathematical model of functioning of a new piezoelectric membrane (MDS) actuator with double spiral (DS) electrodes on the upper and/or lower surfaces of a thin piezoelectric layer with axisymmetric and periodic (with a small period) in radial coordinate mutual reversed electric polarization are presented. The polarization of the layer was realized as a result of connecting the polarizing electric voltage of the appropriate value to the outputs of the double spirals of the electrodes. The electrodes of each (upper and lower) double spiral of the MDS-actuator are made in the form of electrodeposited ribbon coatings on the surfaces of the piezoelectric layer in close proximity to each other (due to the small spiral pitch) to create high values of electric field strength along the lines of force in localized areas of the piezoelectric layer between them when an alternating or constant control electric voltage is connected to the electrodes, in particular, with positive and negative values of the electrical potentials. Importantly, the electric field force lines and, as a consequence, the polarization of the piezoelectric layer of the MDS actuator are oriented mainly along (i.e. towards or against) the radial coordinate of the membrane, in contrast to many conventional actuator schemes. The results of numerical modeling for a circular elastic membrane with piezoelectric actuators installed on its upper and lower surfaces confirmed the effectiveness of the proposed piezoelectric MDS-actuator when it functions according to the “bimorph” scheme, including the use of the proposed new structural element (section) – a piezoelectric “compression ring” MDS at various geometric and control parameters. The effect of a significant increase in the membrane deflection with installed piezoelectric MDS-actuators compared to the use of traditional homogeneous plate piezoelectric actuators of bimorph type for different conditions of the membrane fixation, in particular, stationary (rigid) fixation of its center is revealed. For a hybrid piezoelectric MDS-actuator including independent concentric round and circular (i.e. “compression ring”) sections, the non-monotonic nature and numerical analysis of the nonlinear dependence of the largest deflection at the center of a hinge-immobile membrane fixed at the edge on the ratio of the radii of its round and circular MDS sections were revealed. The cases in which the effect of the “compression ring” is manifested, i.e. when the maximum deflection of a membrane with the “compression ring” exceeds the best possible value of the deflection of this membrane without its use in the traditional “bimorph” scheme, are identified. The new piezoelectric MDS-actuator can be used in micromechanics, controlled optics, sensor technology, acoustics, in particular, in the manufacture of piezoelectric acoustic or sensor elements of membrane type, electromechanical transducers for vibration energy collection.

Unsupervised clustering analysis-based characterization of spatial profiles of inaccuracy in apparent diffusion coefficient values with varying acquisition plan orientation and diffusion weighting gradient direction - A large multicenter phantom study.

This large multicenter study of 37 magnetic resonance imaging scanners aimed at characterizing, for the first time, spatial profiles of inaccuracy (namely, Δ-profiles) in apparent diffusion coefficient (ADC) values with varying acquisition plan orientation and diffusion weighting gradient direction, using a statistical approach exploiting unsupervised clustering analysis.&#xD;A diffusion-weighted imaging (DWI) protocol (b-value: 0-200-400-600-800-1000 s/mm2) with different combinations of acquisition plan orientation (axial/sagittal/coronal) and diffusion weighting gradient direction (anterior-posterior/left-right/feet-head) was acquired on a standard water phantom. For each acquisition setup, Δ-profiles along the 3 main orthogonal directions were characterized by fitting data with a second order polynomial function (ar2+br+c) . Moreover, for each Δ-profile, the maximum minus minimum of the fitting function (δmax) was calculated. &#xD;The parametersa,b,c, andδmaxshowed some significant variations between scanner systems by different manufacturers or with different static magnetic field strengths, as well as between different acquisition/estimation setups. Unsupervised clustering analysis showed two evident clusters with significantly different values of parameter a (p< 0.0001), which can be grouped by acquisition protocol/Δ-profile direction but not scanner system.&#xD;The results of ∆-profiles confirm an appreciable inter-scanner variability in ADC measurement and corroborate the importance of guarantying the reliability of ADC estimations in clinical or research studies, considering for each scanner system the specific acquisition sequence in terms of acquisition plan orientation and diffusion weighting gradient direction.

Reproducibility of liver ADC measurements using first moment optimized diffusion imaging.

Cardiac-induced liver motion can bias liver ADC measurements and compromise reproducibility. The purpose of this work was to enable motion-robust DWI on multiple MR scanners and assess reproducibility of the resulting liver ADC measurements. First moment-optimized diffusion imaging (MODI) was implemented on three MR scanners with various gradient performances and field strengths. MODI-DWI and conventional Stejskal-Tanner monopolar (MONO) DWI were acquired in eight (N = 8) healthy volunteers on each scanner, and DWI repetitions were combined using three different averaging methods. For each combination of scanner, acquisition, and averaging method, ADC measurements from each liver segment were collected. Systematic differences in ADC values between scanners and methods were assessed with linear mixed effects modeling, and reproducibility was quantified via reproducibility coefficients. MODI reduced left-right liver lobe ADC bias from 0.43 × 10-3 mm2/s (MONO) to 0.19 × 10-3 mm2/s (MODI) when simple (unweighted) repetition averaging was used. The bias was reduced from 0.23 × 10-3 mm2/s to 0.06 × 10-3 mm2/s using weighted averaging, and 0.14 × 10-3 mm2/s to 0.01 × 10-3 mm2/s using squared weighted averaging. There was no significant difference in ADC measurements between field strengths or scanner gradient performance. MODI improved reproducibility coefficients compared to MONO: 0.84 × 10-3 mm2/s vs. 0.63 × 10-3 mm2/s (MODI vs. MONO) for simple averaging, 0.66 × 10-3 mm2/s vs. 0.50 × 10-3 mm2/s for weighted averaging, and 0.61 × 10-3 mm2/s vs. 0.47 × 10-3 mm2/s for squared weighted averaging. The feasibility of motion-robust liver DWI using MODI was demonstrated on multiple MR scanners. MODI improved interlobar agreement and reproducibility of ADC measurements in a healthy cohort.

BIOM-23. DEUTERIUM METABOLIC IMAGING OF RESPONSE TO PRECISION THERAPY IN BRAF-V600E MUTANT GLIOMAS

Abstract Oncogenic BRAF-V600E mutations are frequently observed in low- and high-grade gliomas in adults and children. BRAF transduces signals from receptor tyrosine kinases to downstream effectors such as MEK1/2 and ERK, which regulate both metabolism and proliferation. The combination of the BRAF-V600E inhibitor dabrafenib and the MEK1/2 inhibitor trametinib (Dab/Tra) improves patient survival. However, there is considerable variation in the durability of response, underscoring the need for early biomarkers of treatment response. Magnetic resonance imaging (MRI), which is the gold standard for patient management, does not reliably assess response to therapy. Since the BRAF/MEK pathway regulates metabolism, the goal of this study was to determine whether Dab/Tra induces alterations in glucose metabolism that can be leveraged for non-invasive imaging. Using gene expression profiling, in vivo metabolomics, and stable isotope tracing, we show that Dab/Tra downregulates glucose metabolism to lactate in human and murine BRAF-V600E mutant models (AM38, 2341). In vivo infusion with [U-13C]-glucose in mice bearing intracranial AM38 tumors confirmed that Dab/Tra downregulates glucose metabolism to lactate in the tumor in vivo. Mechanistically, Dab/Tra destabilizes hypoxia inducible factor-1a and downregulates glycolytic genes, including SLC2A1, HK2, PFKFB3, and LDHA. Deuterium metabolic imaging (DMI) is a novel, clinical stage method of tracing glucose metabolism in vivo. Our studies show that lactate production from [6,6-2H]-glucose is reduced in AM38 and 2341 cells treated with Dab/Tra. Importantly, lactate production is reduced within 48 h of treatment with Dab/Tra in mice bearing intracranial AM38 tumors at the clinical magnetic field strength of 3T. Furthermore, this drop in 2H-lactate production is observed in the absence of MRI-detectable volumetric alterations and is predictive of extended survival. Collectively, our studies mechanistically link BRAF/MEK inhibition with downregulation of glycolysis and identify [6,6’-2H]-glucose as a novel contrast agent for imaging early response to therapy. Clinical translation of our studies will enable precision imaging of response to therapy for patients with BRAF-mutant gliomas.

NIMG-19. DEUTERIUM METABOLIC IMAGING INTERROGATES THE HISTONE H3K27M MUTATION AND PROVIDES AN EARLY READOUT OF RESPONSE TO THERAPY IN DIFFUSE MIDLINE GLIOMAS

Abstract Diffuse midline gliomas (DMGs) are lethal primary brain tumors in children that are driven by recurrent lysine 27 to methionine mutations in histone H3 (H3K27M). Radiation is standard of care and the imipridones ONC201 and ONC206 have emerged as promising therapies for DMG patients. Although magnetic resonance imaging (MRI) is the mainstay for patient management, it does not reliably assess response to therapy. Therefore, the goal of this study was to identify metabolic alterations induced by H3K27M that can be leveraged for non-invasive metabolic imaging of DMGs. Using patient-derived (BT245, SF8628, SF7761, SU-DIPG-6, DIPG7, SU-DIPG-13) and syngeneic (26-B7, 26-C2) models, we show that the H3K27M mutation upregulates glucose metabolism via glycolysis to lactate. Silencing the H3K27M mutation reduces glycolytic capacity and depletes lactate, an effect that is associated with downregulation of rate-limiting glycolytic enzymes and transporters (SLC2A1, HK2, PFKFB3, PGK1, SLC16A3). Deuterium metabolic imaging (DMI) is a novel, clinical stage method of tracing glucose metabolism in vivo. Our studies show that lactate production from [6,6-2H]-glucose is spatially localized to tumor vs. normal brain in mice bearing intracranial BT245, SF8628 or 26-B7 xenografts at clinical magnetic field strength (3T). Lactate production from [6,6’-2H]-glucose is reduced in patient-derived (BT245, SF8628, SF7761) or syngeneic (26-B7) cells treated with radiation, ONC201 or ONC206. Importantly, lactate production is reduced at an early timepoint (5 days) following treatment with ONC206, when changes cannot be detected by MRI, in mice bearing intracranial BT245 or SF8628 xenografts. Collectively, our studies link the H3K27M mutation with elevated glycolysis and identify [6,6’-2H]-glucose as a safe, orally administered contrast agent for visualizing the metabolically active lesion and for imaging early response to therapy in DMGs in vivo. Clinical translation of our studies will provide physicians with a much-needed tool to determine whether DMG patients are responding to standard and experimental therapies under development.

Semi-analytical modeling AC loss of a flat stack of Y-Ba-Cu-O tapes

We propose semi-analytical models to compute alternating current (AC) power loss in a stack of N high-temperature superconductor YBa2Cu3O7−x (or Y–Ba–Cu–O) tapes subjected to a time-varying magnetic field perpendicular to the tapes with zero transport current. The models take into account screening of the interior superconducting tapes of the stack from the external magnetic field. We validate the results by experiments carried out at temperature T=77.2K under an applied magnetic field with the amplitude of its induction Bext=0.57T and frequencies up to 110 Hz. As follows from our models, the AC loss per tape in stacks of N tapes decreases with N in agreement with experiments. The approach is extended to compute the AC loss for lower temperatures, larger magnetic fields strengths, and for frequencies up to several kHz. These studies are important for understanding and predicting the AC loss for contemporary motors and generators.