You have accessJournal of UrologyBladder and Urethra: Anatomy, Physiology and Pharmacology I1 Apr 2010196 TRPV1 RECEPTORS IN THE URINARY BLADDER PLAY A ROLE IN COLON-BLADDER CROSS-SENSITIZATION INDUCED BY EXPERIMENTAL COLITIS Chao Qin, Beverly Greenwood-Van Meerveld, Robert Foreman, and Anna Malykhina Chao QinChao Qin Oklahoma City, OK More articles by this author , Beverly Greenwood-Van MeerveldBeverly Greenwood-Van Meerveld Oklahoma City, OK More articles by this author , Robert ForemanRobert Foreman Oklahoma City, OK More articles by this author , and Anna MalykhinaAnna Malykhina Glenolden, PA More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.252AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Cross-sensitization among pelvic organs underlies chronic pelvic pain of unknown etiology. Our previous studies showed that acute experimental colitis leads to hyperexcitability of lumbosacral spinal neurons receiving input from the urinary bladder. Recent studies suggest the involvement of TRPV1 signaling cascades in the development of colon-bladder cross-talk. The objective of this study was to test the hypothesis that desensitization of TRPV1 receptors in the urinary bladder can minimize the effects of acute pelvic inflammation on the excitability of spinal neurons with the urinary bladder input. METHODS Extracellular potentials of single neurons from lumbosacral (L6-S2) spinal cord were recorded and their responses to urinary bladder distension (UBD, 1.5 ml, 20 sec) were examined in male rats. Desensitization of TRPV1 receptors was achieved by intravesical application of resiniferatoxin (RTX, 100 nm, 30 min). Colonic inflammation was induced by intracolonic instillation of trinitrobenzene sulfonic acid (TNBS, 12.5 mg in 50% ethanol). RESULTS Duration of excitatory responses of lumbosacral spinal neurons responding to UBD (1.5 ml, 20s) in rats with acute (4-7 days) colonic inflammation was significantly shortened in the group with RTX pretreatment (25.3±1.5s, n=49) compared to the control group (35.1±4.2s, n=43, P≤0.05). Duration of long-lasting excitatory responses of the bladder projecting spinal neurons during acute colitis was significantly reduced by RTX from 52.9±6.6 s (n=21, saline group) to 34.4±2.1 s (n=21, p≤0.05). High-threshold responses to UBD were more frequently observed in rats after combined application of RTX followed by TNBS 50 of 73 (68.5%) versus 32 of 64 cells (50% in control group, p≤0.05). Desensitization of TRPV1 receptors in the urinary bladder prior to acute colitis increased the number of spinal neurons with large somatic fields from 22.7% (acute colitis) to 58.2% (pretreatment with RTX followed by TNBS, p≤0.01) in the RTX+TNBS group). No significant difference in characteristics of spinal neuronal activity was found between groups with and without intravesical RTX instillation in rats with long-term TNBS-induced colitis (29-33 days). CONCLUSIONS The results of our study provide solid evidence that desensitization of TRPV1 receptors in one of the pelvic organs limits the development of viscerovisceral cross-talk but causes an enhanced spinal neuronal response to somatic stimulation. © 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e77-e78 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Chao Qin Oklahoma City, OK More articles by this author Beverly Greenwood-Van Meerveld Oklahoma City, OK More articles by this author Robert Foreman Oklahoma City, OK More articles by this author Anna Malykhina Glenolden, PA More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...