Oak (Quercus) hybrids were created using over 40 diverse parent species. The developed hybrids were used as stock plants and asexually propagated annually over four years. This was done to measure the effectiveness of a modified stool bed layering technique on diverse members of the oak genus, and this study is part of a long-term project to select superior urban-tolerant oak hybrids for introduction as named cultivars into the nursery industry. The number of shoots produced by a stock plant each year and the probability for those shoots to root were found to vary between different maternal parent species. Results suggest the shoots of the hybrids that are the progeny of rhizomatous shrub Quercus spp. are more likely to develop roots when propagated using the described technique. This article also identifies and describes in detail a reliable technique to clone oaks. INTRODUCTION Oaks (Quercus spp.) are noted for their sexual infidelity, and natural hybrids commonly occur wherever two interfertile species from the same subgenera grow near each other (Sternberg, 2000). This tendency to hybridize is a useful trait that may be utilized by plant breeders to create hybrids with desirable characteristics such as attractive fall color, tolerance to site conditions, and hybrid vigor. Increasingly, a number of purported hybrid oak selections are becoming available in the nursery industry (JFSchmidt) (Dirr, 2010). Until recent years, relatively few selections of oaks have been made due to the difficulty involved in asexually propagating members of this genus by conventional methods. Oaks are notoriously difficult to bud or graft successfully, which compounds the problem of producing superior selections. Recently, a modified layering technique that incorporates juvenile shoots, etiolation, and treatment with indolebutyric acid (IBA) has shown promise in the asexual propagation of Quercus species (Amissah & Bassuk, 2009) (Hawver & Bassuk, 2000). A study examining the usefulness of this propagation method on numerous hybrid oaks was conducted at the research farms of Cornell University, in Ithaca, New York. The hybrids used represent crosses of over 40 diverse parent species, most of which are in the white oak group (subgenus Quercus). A previous study has shown that the rooting success of oaks propagated asexually using a similar method can differ between species (Amissah & Bassuk, 2007), and preliminary research suggests that the propagation success of individual hybrid oaks would vary depending on the parental species involved in each cross (Gao, 2011). The objective of this research was to measure the effectiveness of the modified stool bed layering technique on diverse members of the genus Quercus, as well as to provide insight into which, if any, parent species consistently yielded hybrid progeny that could be successfully propagated. Additionally, this article identifies and describes in detail a reliable technique to clone oaks. This experiment is part of a long-term project underway at Cornell’s Urban Horticulture Institute to select superior urban-tolerant oak hybrids for introduction as named cultivars into the nursery industry. Along with developing a reliable method to clone oaks, the project currently involves screening the developed hybrids for tolerance to alkaline soils (a common issue in urban landscapes) and making long-term observations on growth and landscape performance. MATERIALS AND METHODS Trees used in this study were hybrids grown from seed that were developed by Peter Podaras at Cornell University in Ithaca, New York (Podaras & Wells, 2008). During the years 2004, 2005, and 2006, controlled crosses were made by pollinating seven species of oak trees growing on the Cornell University campus with pollen from 36 species that are native throughout North America, Europe, and Asia. Table 1 details the number of hybrid seedlings developed from each cross. These seedlings were grown in containers for several years, and during the spring of 2008, 345 unique oak genotypes were planted out in a field of Arkport sandy loam with a pH of 6.2 for future use as stock plants. Starting in spring of 2009, and repeated yearly through 2012, these field-grown stock plants were propagated each year using a modified version of the oak propagation protocol developed by Amissah and Bassuk (2009). Table 1. Hybrid crosses performed and the resulting number of acorn-grown genotypes. Maternal Parent Paternal Parent Number of Genotypes Q. bicolor OPEN POLLINATED 7 Q. sp. (unidentified species) 9 Q. ×bebbiana 5 Q. muehlenbergii × Q. robur 6 Q. affinis 3 Q. aliena 27 Q. austrina 4 Q. chapmanii 4 Q. dentata 7 Q. fabri 14 Q. fruticosa 4 Q. fusiformis 2 Q. gambelii 5 Q. geminata 2 Q. glauca 1 Q. graciliformis 11 Q. libani 3 Q. lyrata 2 Q. macranthera 1 Q. minima 5 Q. mongolica var. grosserata 3 Q. muehlenbergii 22 Q. myrsinifolia 19 Q. phillyreoides 7 Q. polymorpha 4 Q. robur 14 Q. rugosa 13 Q. spinosa 2 Q. turbinella 6 Q. vaseyana 6