Field Mouse Research Articles

Spatiotemporal Variation of Small Mammal Communities in Commercial Orchards across the Small Country

The diversity of small mammal communities is a measure of the sustainability of habitats, especially agricultural ones. Based on 2018–2020 data from 18 sites in Lithuania, we analysed factors related to diversity of such a community, specifically the relative abundances and proportions of common vole, striped field mouse, yellow-necked mouse, and bank vole. We assessed the influence of location (central, northern, eastern, southern, and western parts of the country), habitat type (orchards, berry plantations, control habitats), the year and season. The model explained 14.8–33.4% of the listed parameters with p < 0.005 or higher, with the exception of the dominance index and the proportion of the common vole. Time factor (year and season, p < 0.001) and site location (p < 0.05) had the highest influences, while that of habitat type was less significant. The results of this and the former research suggest that commercial orchards play a role in maintaining the diversity and abundance of small mammal communities in the agrolandscapes.

Molecular Identification of Bacillus Isolated from Korean Water Deer (Hydropotes inermis argyropus) and Striped Field Mouse (Apodemus agrarius) Feces by Using an SNP-Based 16S Ribosomal Marker.

Simple SummaryWildlife is a great concern because of its free-ranging movements. They carry bacterial zoonoses in their feces, such as Bacillus species. In this study, we developed a 16S Bacillus species-specific 16S ribosomal RNA (rRNA) molecular marker for species identification. For discrimination of genetically similar members of Bacillus cereus group, including Bacillus cereus, B. anthrax, and B. thuringiensis, a single nucleotide polymorphism (SNP)-based marker was developed. We altered an artificial base at the 3′-end of SNP sites in each SNP-based 16S rRNA primer sequence to improve the ability of SNP-based primers to bind the PCR template sequence, thereby improving the allele-specific detection of target B. cereus strains. SNP analysis in high-quality DNA sequences could facilitate identification and discrimination of closely related bacterial species.Ambiguous, heterogeneous, endospore-forming Bacillus species, notably Bacillus cereus, often produce fatal toxins that threaten human health. We identified Bacillus from wild animal fecal samples (n = 80), including the Korean water deer (n = 25) and striped field mouse (n = 55). Using traditional culture-based methods, 25 animal fecal samples (31.25%; 25/80) were found to be positive for Bacillus species, whereas using molecular techniques, 19 samples (23.75%; 19/80) were found to be positive for the same. In addition, we designed a Bacillus species-specific 16S ribosomal RNA (rRNA) gene marker and utilized it to identify 19 samples by means of PCR amplification and sequencing, using at least one colony from the 19 Bacillus positive samples. The recovered sequences were matched to sequences of three Bacillus species (B. cereus, B. amyloliquefaciens, and B. megaterium) from the GenBank database. Moreover, the phylogenetic tree generated in this study established specific clades for the Bacillus group. In addition, to differentiate between B. cereus, B. anthracis, and B. thuringiensis, we designed a single nucleotide polymorphism (SNP)-based primer by identifying SNPs in the alignment of 16S rRNA gene sequences of B. cereus group strains. The SNPs were used to design primer sets for discrimination between highly similar species from the B. cereus group. The study could be used in surveillance of agricultural fresh-produce-associated Bacillus outbreaks, for accurate identification of each Bacillus species, and in the development of control measures.

Correction to: The Mitochondrial Genetic Diversity of the Olive Field Mouse Abrothrix olivacea (Cricetidae; Abrotrichini) is Latitudinally Structured Across Its Geographic Distribution

Correction to: The Mitochondrial Genetic Diversity of the Olive Field Mouse Abrothrix olivacea (Cricetidae; Abrotrichini) is Latitudinally Structured Across Its Geographic Distribution

Caudal Vertebral Fragilities Related to Loss of a Tail Part in Two Species of the Japanese Field Mice

In the Japanese field mice, Apodemus argenteus and A. speciosus, loss of a tail part has often been observed under natural conditions. Such loss is considered a way of avoiding damage during physical accidents and attacks. Here, we studied the types of losses, types of caudal breakages, and breakage positions along the tail. The losses mainly consisted of intervertebral breakage (inter-VB) and intravertebral breakage (intra-VB). VB occurred widely along the tail in both Apodemus species and showed no specific tendency for the inter-VB positions. Intra-VB was confirmed at caudal vertebrae of the middle portion of the tail, consisting of vertebrae with lower relative width (minimum width/maximum length in each caudal vertebra) ranges showing a mean of approximately 0.15–0.28 in both Apodemus species. Previous studies in lizards and rodents found autotomic inter-VB and intra-VB to be positioned in a specific small portion of the tail. However, we found that in field mice, inter-VB and intra-VB were widely distributed along the tail without a regular position. Therefore, it is suggested that both inter-VB and intra-VB as self-defense ways in Apodemus appear to be mere torn-off breakages rather than autotomies, due to certain fragilities in intra- and intervertebral structures.

Hantavirus Disease: An Update

In addition to the well-known clinical early symptoms of hantavirus disease (fever, flank and abdominal pain as well as arthralgia), unusual neurological changes in the context of infection come into focus. The spectrum of neurological symptoms ranges from transient myopia to severe pareses in the context of Guillain-Barré syndrome. In endemic areas, rapid IgM tests for initial assessment are of certain value for differential diagnosis. For therapeutic approaches, only supportive measures up to transient dialysis are available.Molecular genetic analysis and comparison of hantavirus strains of patients and mice from the same geographical area allowed molecular characterization of different outbreak regions. In the meantime, the Puumala viruses of the main outbreak regions in Germany are molecularly well characterized; therefore, the nucleotide sequence of the virus strain detected in a patient makes it possible to draw conclusions about the geographic region where the patient's infection took place.The human pathogenic hantaviruses being prevalent in Germany are the Puumala virus (reservoir: bank vole) and the Dobrava-Belgrade virus, genotype Kurkino (reservoir: striped field mouse). Recently, the molecular detection of further hantaviruses in patients with hantavirus disease was achieved. It can be concluded that also the Seoul virus (reservoir: rats) and the Tulavirus (reservoir: field mouse and related species) occasionally cause hantavirus disease in Germany.New results revealed that human infections can occur not only by the generally accepted route of inhalation of virus-containing aerosols, but also by ingestion of virus-containing materials.For patients with hantavirus infection or disease, it can be assumed that they are not infectious for their environment. A new systematic review could not confirm a human-to-human transmission previously postulated for South American hantaviruses.While all known human pathogenic hantaviruses are transmitted by rodents, other hantaviruses have been recently detected in shrews, moles, and bats. The clinical significance of these new viruses is quite unclear as yet.

Cocirculation of Leptospira spp. and multiple orthohantaviruses in rodents, Lithuania, Northern Europe.

In Europe, zoonotic Leptospira spp. and orthohantaviruses are mainly associated with specific rodent hosts. These pathogens cause febrile human diseases with similar symptoms and disease progression. In Lithuania, the presence of Dobrava-Belgrade orthohantavirus (DOBV), Tula orthohantavirus (TULV) and Leptospira spp. in rodent reservoirs is still unknown, and Puumala orthohantavirus (PUUV) was detected in bank voles (Clethrionomys glareolus) at only one site. Therefore, we collected and screened 1617 rodents and insectivores from Lithuania for zoonotic (re-)emerging Leptospira and orthohantaviruses. We detected Leptospira DNA in six rodent species, namely striped field mouse (Apodemus agrarius), yellow-necked mouse (Apodemus flavicollis), bank vole, common vole (Microtus arvalis), field vole (Microtus agrestis) and root vole (Microtus oeconomus). Leptospira DNA was detected with an overall mean prevalence of 4.4% (range 3.7%-7.9% per rodent species). We detected DOBV RNA in 5.6% of the striped field mice, PUUV RNA in 1% of bank voles and TULV RNA in 4.6% of common voles, but no Leptospira DNA in shrews and no hantavirus-Leptospira coinfections in rodents. Based on the complete coding sequences of the three genome segments, two distant DOBV phylogenetic lineages in striped field mice, one PUUV strain in bank voles and two TULV strains in common voles were identified. The Leptospira prevalence for striped field mice and yellow-necked mice indicated a significant negative effect of the distance to water points. The detection of (re-)emerging human pathogenic Leptospira and three orthohantaviruses in rodent reservoirs in Lithuania calls for increased awareness of public health institutions and allows the improvement of molecular diagnostics for pathogen identification.

The elemental composition of small mammals in a commercial orchard–meadow system

We analyze concentrations of various elements in small mammals from commercial orchards and berry plantations and test differences between them depending on species, individual body mass, age, gender, type and age of crop and intensity of applied agricultural measures. Skinned front legs (muscle and bones) were used to register the presence and concentration of Ca, Cd, Mo, Ni, Se, Ag, As, Be, Bi, Co, Cr, Cu, Fe, Ga, Mg, Mn, Pb, Sr, U, V and Zn. The most abundant species were common vole (Microtus arvalis), yellow-necked (Apodemus flavicollis) and striped field (A. agrarius) mice. The maximum recorded concentrations exceeded the minimums by 1.7–7.7 times in Mg, Zn, Cu, Ga, Ni and Ca, and 11.0–23.2 times in Mn, Be, Mo, Co, Sr, V, Pb and As. The hypothesis that the use of fertilization and pesticides in commercial orchards should induce differences in the elemental concentrations between crop areas and control habitats was confirmed by the higher concentrations of Cu, Mn, Bi, Co, Cr, Fe, Ni, Sr and Pb in rodents from the crop areas. Spatially controlled generalized linear mixed model confirmed the cumulative influence of species and crop, explaining 30–80% of the distribution of Ca, Ni, Co, Cu, Ga, Mn, Pb and U. The effect of species and the age of the individual was significant for Ni, Co, Cu, Fe, Ga, Mn and Pb, while effect of gender was not expressed. Depletion of Cu in older individuals was found in all three species. With species as a grouping factor, the effect of crop type and the intensity of agricultural practices were significant factors in the accumulation of Ca, Ni, Co, Cu, Ga, Mn and Pb, while effect of crop age was not expressed. The obtained elemental concentrations in rodents indicated orchards to be cleaner than heavy polluted areas.

Responsive strategies of three sympatric small rodents to the altitudinal effects on microhabitats

Abstract This study evaluated the effects of altitudinal gradients on small-rodent populations and microhabitat conditions. We selected three altitudinal bands: lowland, mid-land, and highland. We captured three small-rodent species, the striped field mouse (Apodemus agrarius), the Korean field mouse (A. peninsulae), and the red-backed vole (Myodes regulus). A. agrarius preferred microhabitats with dense ground vegetation and sparse mid-story vegetation, basal area, downed trees, and stone coverage. A. peninsulae utilized ground vegetation, overstory vegetation, and downed trees. M. regulus occupied microhabitats with abundant stone coverage and little ground vegetation coverage. Ground vegetation coverage was higher in the mid-land. The lowland was characterized by high understory vegetation, bulky downed trees, and high stone coverage. A. agrarius mostly occupied the mid-land, whereas A. peninsulae and M. regulus mainly inhabited the lowland. Our results show that each small-rodent species had its own strategy for utilizing the key habitat factors in altitudinally affected microhabitats and for reducing competition among the populations. This finding contributes to improving knowledge of the ecological altitudinal features of microhabitat conditions, as well as the altitudinal distributions and abundances of three small-rodent species.

Description of the Nymphal Stages of Hoplopleura affinis (Anoplura: Hoplopleuridae) from the Striped Field Mouse Apodemus agrarius with a Global Checklist of Hoplopleura Species from the Genus Apodemus.

Simple SummarySucking lice are blood-feeding, external parasites of mammals. To date, approximately 540 species have been discovered, occurring in 830 hosts. New species are discovered every year, and the total number is estimated at 1500. The discovery of a species is associated with a detailed description of morphological characteristics. Most descriptions concern only adult specimens. The present study adds to the knowledge by characterizing nymphs of Hoplopleura affinis, a species that parasites the striped field mouse Apodemus agrarius, a common rodent in Europe and Africa. In addition, a checklist of Hoplopleura species parasitizing members of the genus Apodemus was compiled.The genus Hoplopleura is the most speciose genus of sucking lice observed parasitizing rodents and lagomorphs (pikas). Despite the fact that the majority of Anoplura are believed to be monoxenic, many species within Hoplopleura may be oligoxenic. In addition, the occurrence of more than one parasite species per host species on individuals has been confirmed. As such, a precise species identification of the parasite, especially of the nymphs, is of high significance. The study is based on the material of 245 sucking louse specimens taken from 179 individuals of the striped field mouse Apodemus agrarius collected between 2008 and 2017. The study employs scanning microscopy to provide superior quality resolution of the studied traits. The study presents the first record of the characters of the nymphal stages of H. affinis, one of the common Eurasian species of the genus. Additional aspects of the biology and the host–parasite relationship of H. affinis are presented, e.g., female, male and nymphs of lice, showing different preferences in the choice of location (topography) on the host body. In addition, a global checklist has been made of all the species of Hoplopleura found parasitizing rodents of the genus Apodemus. Generally, the ranges of the occurrence of lice of this genus coincide with the geographic distribution of typical hosts, although this has not always been confirmed by local studies.

The Mitochondrial Genetic Diversity of the Olive Field Mouse Abrothrix olivacea (Cricetidae; Abrotrichini) is Latitudinally Structured Across Its Geographic Distribution

The Mitochondrial Genetic Diversity of the Olive Field Mouse Abrothrix olivacea (Cricetidae; Abrotrichini) is Latitudinally Structured Across Its Geographic Distribution

Synanthropic rodent fauna of Eastern Transbaikalia in the early 21st century

The article provides information on the synanthropic rodent fauna of Eastern Transbaikalia. In 2017–2020 the city of Chita, the town of Baley and small settlements of Zabaykalsky krai were surveyed. True synanthropes: the house mouse and the brown rat are most abundant in residential landscapes. Natural populations of the house mouse are typical for coastal biotopes of the steppe zone, and the brown rat is typical for forest-steppe zone of Zabaykalsky krai. In Chita (administrative centre of Zabaykalsky krai) the share of the brown rat in rodent communities has considerably increased in recent decades. There is an extension of the brown rat’s habitat in the area of Baikal-Amur Mainline. Optional synanthropes: striped field mouse, striped dwarf hamster, Maximowicz’s vole, and narrow-headed vole are less important in residential landscapes of the region. In the beginning of the 21st century the field mouse is extending its habitat to the disjunct zone to the west of Transbaikalia: it is now common in the residential area of the town of Baley. Penetration of another optional synanthropic species recorded in the neighbouring Siberian regions – the East European vole – has not been detected.

Intestinal parasites of Apodemus mystacinus along altitudinal stratification of Ibrahim River, Mount Lebanon.

This study documents the diversity of intestinal parasites in the eastern broad-toothed field mouse (Apodemus mystacinus) along the altitudinal gradient of Ibrahim River in Mount Lebanon during the spring and autumn of 2017. A total of 190 A. mystacinus were trapped in seventeen sites (6 riparian, 11 non-riparian) and examined for intestinal parasites. Eight intestinal parasites were identified including Heligmosomoides polygyrus, Trichuris muris, Syphacia frederici, Protospirura muris, and Aspiculuris tetraptera (nematodes), Hymenolepis diminuta (cestode), Brachylaima spp. (trematode), and Eimeria alorani (coccidian). Most of the trapped mice (85%) were infected with intestinal parasites and 38.84% showed concurrent infection with at least two parasitic species. The season had a significant effect on mice infection with Syphacia frederici, and mice are most likely to have infection with this nematode in spring season. The vegetation zone had a significant overall impact on mice infection with the nematodes Heligmosomoides polygyrus and Trichuris muris while gender did not influence significantly mouse infection with intestinal parasites. This is the first study that has been carried out to identify the intestinal parasite community in A. mystacinus of Ibrahim River region. The findings pave the way for future studies on intestinal parasites in rodents and the environmental variations affecting their dynamics.

ПОЛИМОРФИЗМ И УРОВЕНЬ ДИФФЕРЕНЦИАЦИИ ОСТРОВНЫХ И МАТЕРИКОВЫХ ПОПУЛЯЦИЙ ПОЛЕВОЙ МЫШИ APODEMUS AGRARIUS ЮГА ДАЛЬНЕГО ВОСТОКА РОССИИ ПО ДАННЫМ АНАЛИЗА МИКРОСАТЕЛЛИТОВ

An assessment of polymorphism and the level of differentiation in insular and mainland populations of the field mouse was carried out. Specimens of four islands in the Peter the Great Bay of the Sea of Japan and four regions on the mainland of the of the Russian Far East South were tested. Nuclear markers of 5 microsatellite loci were studied. The results demonstrate higher allelic diversity in the mainland part of the range, with a significantly higher level of differentiation in the insular part of the range.

Hemorrhagic Fever with Renal Syndrome: Literature Review, Epidemiology, Clinical Picture and Pathogenesis

Hantaviruses can cause two types of infections in humans: hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome. The old world hantaviruses, primarily Hantaan virus (HTNV), responsible for causing HFRS occurs endemically in Asia and Europe. Apodernus agraricus, a striped field mouse, is being considered as main host reservoir for HTNV. Infection in humans is typically accidental and occurs when virus-containing rodent excretions such as urine, feces, or saliva are aerosolized. The major clinical manifestations includes increased vascular permeability causing vascular leakage, acute kidney injury and coagulation abnormalities. The case fatality rate of HFRS varies around 5.0 - 10.0% depending on the causative viral agent. The direct effects of viral infection on endothelial cells, as well as the immunological response to the viral infection, have been suggested to play a key role in the pathogenesis of HFRS. This article summarizes the current knowledge of HFRS epidemiology in Korea and around the globe, etiology, host transmission, clinical presentation, pathogenesis, diagnostic techniques, treatment, and prevention.

Corrigendum to: Field Mice: A North Dakota Family Farm Faces the Pernicious Effects of Modern Agribusiness

Corrigendum to: Field Mice: A North Dakota Family Farm Faces the Pernicious Effects of Modern Agribusiness

Infestation and distribution of chigger mites on Chevrieri's field mouse (Apodemus chevrieri) in Southwest China.

Based on a long-term field investigation on chigger mites in southwest China from 2001 to 2019, the present study analyzed the infestation and distribution of chigger mites on the Chevrieri's field mouse (Apodemus chevrieri) in the region. A total of 12,516 individuals of chigger mites were collected from 1981 A. chevrieri mice, and 12,281 chiggers were identified as 107 species, 11 genera and 3 subfamilies in 2 families, which revealed a high species diversity of the mites on A. chevrieri mice. Of 1981 A. chevrieri mice, 633 ones were infested with chiggers with a relatively high overall prevalence (PM = 31.95%), mean abundance (MA = 6.32) and mean intensity (MI = 19.77). Of the 107 chigger species identified from A. chevrieri mice, three ones were the most dominant and they were Leptrombidium scutellare, L. densipunctatum and L. cricethrionis, which showed aggregated distribution among different individuals of the mice. A slightly positive association existed between every two dominant chigger species, which implied that the dominant chigger species tend to co-exist on A. chevrieri. The infestations of A. chevrieri with chiggers varied in different latitudes, altitudes and landscapes and they showed some heterogeneity along different environmental gradients. The logistic regression analysis showed that the risk factors for chigger infestations on A. chevrieri were landscapes, ages and altitudes, which implied that the environmental factors and host ages could influence the infestations of the mice with the mites. A theoretical curve of the species abundance distribution of chigger mites on A. chevrieri was successfully fitted by Preston's lognormal model, suggesting that the species abundance distribution conforms to the lognormal distribution pattern. The expected total species of chigger mites on A. chevrieri was roughly estimated to be 136 species and about 29 rare chigger species were probably missed in the sampling field investigation.

Major Regularities of Transformation of the Small Mammal Ovaries in Unfavourable Environmental Conditions: Ecological and Morphological Aspects

The aim of the study was to determine the patterns of structural and functional organization of the ovaries of female small mammals inhabiting technogenically altered ecosystems.Material and methods. We studied the ovaries of small mammal species belonging to the insectivore and rodent families (common shrew, field and pygmy wood mice, common and bank voles, mole vole, steppe pied) that live in anthropogenically altered ecosystems (zones of influence of ferrous and nonferrous metallurgy, as well as gas processing factory). The resulting material was processed using observational histological, histochemical, immunohistochemical and morphometric tests.Results. The results obtained demonstrated that in technogenically altered ecosystems the intensified reproduction results in a complex of morphofunctional reactive and adaptive changes in the ovaries of females of the studied species. The size of the ovaries was reduced; the area of the cortical substance was reduced. In the cortex, there was revealed a decrease in the number of follicles varying over a wide range - from a moderate decrease to their almost complete absence. There was found a decrease in the area of the vessels of the microvasculature; this was one of the major reasons for the increased follicular atresia. In follicles of various types, there was an increase in the proportion of cells expressing the proapoptotic protein P53. A decrease in the number of follicles resulted in the connective tissue overgrowth. The presence of cysts lined with epithelium of various heights was revealed in the cortex and medulla. Conclusion. The results obtained evidence that in technogenically altered ecosystems a decreased ovarian reserve is observed in the ovaries of female small mammals; it is associated with a more rapid depletion of the follicle reserve in the cortex due to both - intensification of reproduction and more rapid death of follicles in unfavourable environmental conditions.

Scent, rather than fur pattern, determines predation of mice: an in‐the‐wild experiment with plasticine mouse models

AbstractWood mouse Apodemus sylvaticus and striped field mouse A. agrarius are the most abundant mouse species in Central Europe coexisting in several habitats not only such as in forests but also in rural and urban areas. Their basic colours are similar, but the striped field mouse has a pronounced black stripe on its back. The biological function of this phenotypic difference is unexplored; therefore, our goal was to study the impact of fur pattern as well as the scent on the survival of these species. In our study, we used plasticine models mimicking wood mouse and striped field mouse. For imitating mouse scent, cotton pads saturated with mouse urine were placed under the models. In spring 2016, 200 plasticine mice divided into four types (non‐striped with scent, non‐striped without scent, striped with scent, striped without scent) were placed randomly in four sampling sites in the mid‐sized city, Pécs, Hungary. During 4 weeks of exposure, we recorded predation on 71 plasticine models. Birds (51%) and large mammals (25%) were the most common predators, and in 24% of the cases, the models disappeared. Considering predation only by large mammals, we found that the daily survival rate of both striped and non‐striped models with scent decreased in comparison to models without scent. Thus, large mammalian predators could discover plasticine models sooner than avian predators, which suggests a strong impact of scent. Despite the high rate of bird predation, we did not detect an effect of fur pattern suggesting that it may not be related to survival but might have other functional roles. With the dummy mouse experiment, we showed that fur pattern may play a minor role in mice detection by predators, thus further studies are needed to discover its ecological function.

A Report of Chigger Mites on the Striped Field Mouse, Apodemus agrarius, in Southwest China.

Based on the field investigations in 91 investigation sites (counties) in southwest China between 2001 and 2019, the present paper reported the chigger mites on A. agrarius mice in southwest China for the first time by using a series of statistical methods. From 715 striped field mice captured in 28 of 91 investigated sites, only 255 chiggers were collected, and they were identified as 14 species, 6 genera in 3 subfamilies under 2 families. Of 715 A. agrarius mice, only 24 of them were infested with chigger mites with low overall prevalence (PM=3.4%), overall mean abundance (MA=0.36 mites/host) and overall mean intensity (MI=10.63 mites/host). The species diversity and infestation of chiggers on A. agrarius were much lower than those previously reported on some other rodents in southwest China. On a certain species of rodent, A. agrarius mouse in southwest China seems to have a very low susceptibility to chigger infestations than in other geographical regions. Of 14 chigger species, there were 3 dominant species, Leptotrombidium sialkotense, L. rupestre and Schoengastiella novoconfuciana, which were of aggregated distribution among different individuals of A. agrarius hosts. L. sialkotense, one of 6 main vectors of scrub typhus in China, was the first dominant on A. agrarius. The species similarity of chigger mites on male and female hosts was low with CSS=0.25, and this reflects the sex-bias of different genders of A. agrarius mice in harboring different chigger species.

Human Wildlife Conflict and Impacts on Livelihood: A Study in Community Forestry System in Mid-Hills of Nepal

Human wildlife conflict (HWC) impacts the livelihood of many rural communities worldwide. This study investigated the impact of HWC on people living near community forests (CF) in Nepal. Using databases provided by the Division of Forest Offices and data obtained from surveys between October 2019–March 2020, we quantified the financial loss of HWC to the local people. Between 2015 and 2019, 3315, or 27%, of the livestock owned by the survey respondents were killed by wild predators in the Kaski and Tanahun Districts. Chicken (Gallus spp.) was the most common prey taken (80%), followed by sheep (Ovis spp.) and goats (Capra spp.) (15%), cows (Bos spp.) (2%), pigs (Sus spp.) (2%), and buffalo (Bubalus spp.) (1%). Leopards (Panthera pardus) were the primary predators, followed by golden jackals (Canis aureus), jungle cats (Felis chaus), yellow-throated martens (Martes flavigula), and Himalayan black bears (Ursus thibetanus). The financial loss of livestock during this period was USD $115,656.00, equivalent to USD $142.61 per household. Crops were also damaged and eaten by wildlife, and 2165 crop-raiding events were recorded between 2015 and 2019. Rice (Oryza sativa), followed by maize (Zea mays), millet (Panicum miliaceum), and potatoes (Solanum tuberosum) were the main crops lost. Rhesus monkeys (Macaca mulatta) were the most common crop raiders, causing 74% of the damage, followed by Indian field mice (Mus booduga) (12%). From 2015 to 2019, crop losses equated to USD $83,424.00. Forest regeneration on abandoned agricultural land expanded wildlife habitats, enabling wild animals to come within reach of human settlements, which increased the likelihood of HWC events. Although the success of the community forest restoration program resulted in increased forest-cover, marginally increasing biodiversity, the reduced distance between human settlements and wildlife habitat, compounded by a lack of natural prey, may have unwittingly exacerbated HWC in this region. We recommend surveying predator and prey populations in the forest habitat, and implementing a habitat management program to improve prey populations within the community forests. Meantime, we propose establishing a financial relief and insurance program for crop and livestock losses at the local community level to alleviate any financial difficulties to the local communities caused by HWC.