Ficus Benjamina Research Articles

Phytoremediation potential of potted plant species against vehicular emissions

Urbanization and industrialization are exponentially deteriorating air quality, ecosystems, and human health. Phytoremediation is cost cost-effective, sustainable, and nature-based solution against air pollution. This study is designed to evaluate four species, Chlorophytum comosum, Rhapis excelsa, Spathiphyllum wallisii, and Ficus benjamina for their phytoremediation potential. The experimental setup consisted of a sealed chamber to place potted plants and equipment, it was also connected to the vehicular exhaust pipe. The Air Pollution Tolerance Index was highest for F. benjamina (12.19) and lowest for Rhapis excels (8.58). C. comosum has the highest VOC removal efficiency (90%, 0.172 ppm h−1). NOx remediation was highest by F. benjamina with 0.057 ppm h−1 (77%) removal efficiency. SOx and CO were remediated more efficiently by C. comosum, as 89%, (0.18 ppm h−1) and 80% (0.23 ppm h−1), respectively. R. excelsa reduced a higher concentration of NH3 (77%, 0.06 ppm h−1) compared to other species. R. excelsa and S. wallisii may serve as bio-indicator species. These findings provide a sustainable, natural, economical, and eco-friendly way to mitigate air pollution. F. benjamina and C. comosum are suitable species for urban landscapes, green spaces, urban plantations, and green walls to curb air pollutants due to traffic and industries.

First detection of alfalfa mosaic virus in Ficus benjamina in Chile.

Ficus benjamina, commonly known as ficus or weeping fig, is an ornamental tree from Southeast Asia in the Moraceae family. It is an evergreen species grown as an interior plant worldwide, prized for its glossy green leaves, gray stem, and multiple branches. F. benjamina can be pruned and kept as a small shrub when grown indoors, with most commercially available cultivars in the ornamental industry derived from sports and propagated cuttings (Chen et al., 2010). In October 2022, symptoms indicative of alfalfa mosaic virus (AMV) infection, such as intense bright yellow spots, mottling and chlorosis were observed in leaves of F. benjamina growing in office environments in Santiago, Chile (supplementary Fig.1). Seven symptomatic and three asymptomatic plants were sampled and traced back to the same plant nursery, and only leaf tissue samples from the symptomatic plants tested positive for the presence of the AMV using enzyme-linked immunosorbent assay (ELISA) (Agdia Inc., Elkhart, IN). These results were verified using reverse transcription (RT)-PCR with specific primers AMV-F and AMV-R, targeting a region of the AMV CP gene (Xu and Nie, 2006). AMV was detected in all symptomatic leaf samples, producing 351-bp amplicons in the RT-PCR assay for all samples that were positive to AMV by ELISA. No amplification product was observed when seronegative samples or non-template control was used as templates in the RT-PCR assays. Three RT-PCR amplicons were directly sequenced in both directions. BLAST analysis of these sequences showed 100% nucleotide sequence identity to an AMV isolate previously reported (GenBank Accession No. KX458469), confirming the detection of AMV in F. benjamina. This pathogen causes disease in several species, including ornamentals plant such as Viburnum tinus L. (Peña et al., 2011), peony (Belardi and Rubies, 2003), phlox (Holcomb et al., 2006), and weeds such as Cayratia japonica (Thunb.) Gagnep, Justicia procumbens L. and Veronica persica Poir. (Gao et al., 2020). F. benjamina is among the most popular ornamental indoor plants in Chile, so nurseries should ensure AMV-free plants to restrict disease incidence and prevent the spread of this virus. However, Chile lacks specific regulations for ornamental nurseries, making adherence to pest and disease recommendations voluntary, and only imported plant material requires a sanitary certificate.

Preferensi Masyarakat terhadap Tanaman Identitas di Jalur Hijau Tepi Jalan Kota Yogyakarta

Philosophical and native plants have ecological, aesthetic, and air pollution tolerant functions, and have historical value as constituents of Green Open Spaces (GOS). Plants in the green belt of the Imaginary Line of Yogyakarta are different from other green belt plants, because there are special features. the preferences of the Yogyakarta City community are carried out to choose the right plants, so as to create a good ecosystem on the green belt of Yogyakarta City roads. The purpose of this study was to identify plants to recommendations from people's preferences in choosing green belt plants in the City of Yogyakarta. the research method for selecting respondents used the Slovin method and in-depth interviews with cultural experts from the Yogyakarta Palace, and further tested using the Spearman correlation test. The results showed that of the 33 philosophical and native plants, the physiological characteristics of the plants had a percentage of 8.9%, namely Ficus benjamina L. as a philosophical plant and Chrysopyllum cainito L. 14.6% as a native plant. Visual plant characteristics that have a score of 7 are Murraya paniculata (L.) Jack and Aegle marmelos (L.) Corrêa.

Development and Evaluation of Real-Time Quantitative PCR Assays for Detection of Phellinus noxius Causing Brown Root Rot Disease.

Brown root rot disease (BRRD) is a highly destructive tree disease. Early diagnosis of BRRD has been challenging because the first symptoms and signs are often observed after extensive tissue colonization. Existing molecular detection methods, all based on the internal transcribed spacer (ITS) region, were developed without testing against global Phellinus noxius isolates, other wood-decay fungi, or host plant tissues. This study aimed to develop SYBR Green real-time quantitative PCR (qPCR) assays for P. noxius. The primer pair Pn_ITS_F/Pn_ITS_R targets the ITS, and the primer pair Pn_NLR_F/Pn_NLR_R targets a P. noxius-unique group of homologous genes identified through a comparative genomics analysis. The homologous genes belong to the nucleotide-binding-oligomerization-domain-like receptor (NLR) superfamily. The new primer pairs and a previous primer pair G1F/G1R were optimized for qPCR conditions and tested for specificity using 61 global P. noxius isolates, 5 other Phellinus species, and 22 non-Phellinus wood-decay fungal species. Although all three primer pairs could detect as little as 100 fg (approximately 2.99 copies) of P. noxius genomic DNA, G1F/G1R had the highest specificity and Pn_NLR_F/Pn_NLR_R had the highest efficiency. To avoid false positives, the cutoff quantification cycle values were determined as 34 for G1F/G1R, 29 for Pn_ITS_F/Pn_ITS_R, and 32 for Pn_NLR_F/Pn_NLR_R. We further validated these qPCR assays using Ficus benjamina seedlings artificially inoculated with P. noxius, six tree species naturally infected by P. noxius, rhizosphere soil, and bulk soil. The newly developed qPCR assays provide sensitive detection and quantification of P. noxius, which is useful for long-term monitoring of BRRD status.

A Review on Phytochemistry and Pharmacological Activity of Ficus benjamina

A Review on Phytochemistry and Pharmacological Activity of Ficus benjamina

Enhanced Removal of Chlorpyrifos, Cu(II), Pb(II), and Iodine from Aqueous Solutions Using Ficus Nitida and Date Palm Biochars

This study explores the adsorption efficiency of biochar derived from palm trees and Ficus nitida for the removal of various contaminants, including Cu(II), Pb(II), iodine, and chlorpyrifos from aqueous solutions. Biochar was prepared using a two-step pyrolysis process for date palm biochar and single-step pyrolysis for Ficus nitida biochar. Characterization techniques such as SEM, EDX, and FTIR revealed a significant surface area and a variety of functional groups in both types of biochar, essential for effective adsorption. The date palm biochar exhibited superior adsorption capacities for Cu(II) and Pb(II) ions, achieving efficiencies up to 99.9% and 100%, respectively, due to its high content of oxygen-containing functional groups that facilitated strong complexation and ion exchange mechanisms. Conversely, Ficus nitida biochar demonstrated a higher adsorption capacity for iodine, reaching 68% adsorption compared to 39.7% for date palm biochar, owing to its greater surface area and microporosity. In the case of chlorpyrifos, Ficus nitida biochar again outperformed date palm biochar, achieving a maximum adsorption efficiency of 87% after 24 h of incubation, compared to 50.8% for date palm biochar. The study also examines the effect of incubation time on adsorption efficiency, showing that the adsorption of chlorpyrifos by date palm biochar increased significantly with time, reaching a maximum of 62.9% after 48 h, with no further improvement beyond 12 h. These results highlight the importance of biochar characteristics, such as surface area, pore structure, and functional groups, in determining adsorption efficiency. The findings suggest that optimizing pyrolysis conditions and surface modifications could further enhance the performance of biochar as a cost-effective and sustainable solution for water purification and environmental remediation.

A study on the interaction parameters of charged and uncharged radiation types with some indoor plants

This study investigates how gamma rays, neutrons, and electrons interact with five commonly found indoor plants: Spathiphyllum wallisii (SW), Ficus elastica (FE), Dieffenbachia camilla (DC), Schefflera arboricola (SA), and Ficus benjamina (FB). Utilizing experimental measurements (with HPGe detector), Monte Carlo simulations (GEANT4 and FLUKA), and theoretical calculations (ESTAR and WinXCOM), some radiation interaction parameters for gamma rays, fast neutrons, thermal neutrons, and electrons were determined. Secondary particle generation was also analyzed to provide a comprehensive assessment. The determined linear attenuation coefficients with the help of the WinXCOM are 0.1376, 0.1662, 0.1385, 0.1651 and 0.1698 cm-1 for SW, FE, DC, SA and FB, respectively. The calculated total macroscopic cross sections for indoor plants in the same sample order are 2.0290, 2.0350, 2.0285, 2.0363 and 2.0362 cm-1. Among the investigated plants, FB exhibited the highest gamma ray interaction, while SA and FB showed superior interaction against fast neutrons compared to SW and DC. The findings reveal significant variations in interaction effectiveness and secondary radiation production across these plants, offering valuable insights for radiation safety and environmental health evaluations.

Green synthesis of zero‐valent copper nanoparticles for removal of D‐yellow 119 textile dye from aqueous medium

AbstractZero‐valent copper nanoparticles (CuNPs) were beneficially green synthesized via Ficus Benjamina leaves. Applying scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT‐IR), Ficus Benjamina nano zero‐valent copper (FB‐nZVCu), an innovative adsorbent, was examined. The obtained zero‐valent CuNPs have a size range of 16–18 nm. The removal of D‐yellow 119 dye from textile wastewater was tested using this novel adsorbent. Many operating parameters were examined and tested to control the adsorbent's maximum removal efficiency. These variables included dye concentration, stirring rate, time, pH, and adsorbent dosage. Different adsorption mechanisms have been tested, and the Langmuir isotherm (qmax = 21.83 mg g−1) and (R2 = 0.9993) represent adequate for the adsorption process. The FB‐nZVCu green adsorbent is a promising material for eliminating D‐yellow 119 from simulated and real samples, according to the data obtained and the fruitful analysis. The impact of different operating factors was examined using IBM SPSS Statistics software. They were shown to be responsible for approximately 94% of the factors influencing the removal process.

Old-growth Ficus trees provide soil water and carbon storage to urban greenspaces in a Brazilian metropolis

Urban flooding has been considered one of the most severe natural disasters around the world, and urban greenspaces can provide important flood regulation ecosystem services. In Belo Horizonte (Brazil), the woody vegetation, especially the old-growth Ficus trees, appears to protect an urban park against the flooding of the Arrudas river. Thus, we compared the soil water content, soil water-holding capacity, soil aggregation and porosity among a highly permeable urban park planted with herbaceous and woody species, including Ficus, a semi-permeable parking lot with only Ficus and an impermeable site without trees (Disturbed Site-DS). The soil water content and water holding capacity of the urban park did not differ from that of the Ficus site, but it was lower than DS. These results were correlated with soil organic matter (SOM) content, soil aggregation and porosity, suggesting that Ficus trees play an important role in the hydrological cycle. To understand how the Fiscus species provide such soil permeability, we compared the aboveground plant biomass, soil fertility and soil carbon sequestration (total soil carbon, soil organic matter, humic and fulvic acids and soil isotopic δ13 C) in plots with Ficus and without this species within the park as well as in a preserved urban forest and a disturbed square. The outstanding plant biomass produced by Ficus species explained the high soil carbon sequestration, particularly in humic organic matter, favouring soil aggregation, porosity and water retention. Therefore, Ficus species may be considered an exceptional C-sequestering species, contributing for soil stabilization and the hydrological cycle in urban greenspaces.

Ethnomedical Study of Traditional Medicinal Plants for Cough and Tuberculosis (TB) of the Helong Tribe on Semau Island, Kupang Regency, East Nusa Tenggara

The Helong people in Kupang Regency, NTT Province, have experienced being exposed to cough and tuberculosis (TB). Therefore, it is believed that this community has also long since developed their local knowledge and traditional ways to prevent and treat the disease. In general, this study aims to analyze and find the concept of the Helong Tribe community and ethnomedical practices for the prevention and treatment of cough and tuberculosis (TB). This research is a medical anthropology research using a qualitative research method with an ethnographic approach, which is supported by laboratory research and literature studies. Field research was carried out on Semau Island, NTT Province, involving traditional medicine. The results of the research conducted in the form of interviews with traditional medicine practitioners in the Helong tribe obtained as many as 31 plants used in treatment, and there were 3 plants used to treat cough, including hemorrhagic cough/tuberculosis, namely plants: white banyan (Ficus benjamina L), mesocarp palm fruit (Borassus flabellifer L.) and white flower (Clerodendrum costatum R.Br). The three plants that are suspected to have the ability to treat cough and cough up blood carried out preliminary tests of antibacterial activity against bacteria that generally cause stones, namely Staphylococcus aureus bacteria. The results obtained for ethanol extract of white banyan leaves (Ficus benjamina L) with a concentration of 25% b/v with a diameter of 16.53 mm, 50% b/v with a diameter of 16.33 mm, and 75% b/v with a diameter of 16.56 mm. Mesocarp ethanol extract of palm fruit (Borassus flabellifer L.) At a concentration of 25% b/v with an inhibitory zone diameter of 11.60 mm, a concentration of 50% b/v with an inhibitory zone diameter of 12.30 mm, a concentration of 75% b/v with an inhibitory zone diameter of 13.40 mm, for white flowers (Clerodendrum costatum R.Br) at a concentration of 25%b/v with an inhibitory zone diameter of 9.41 mm, a concentration of 50% b/v of 10.36 mm and a concentration of 75% b/v of 11.24 mm. This means that the three plants have antibacterial activity against Staphylococcus aureus bacteria. Furthermore, for the antibacterial activity test against Mycobacterium tuberculosis bacteria for white banyan plants (Ficus benjamina L.), mesocarp of palm fruit (Borassus flabellifer L.), and white flowers (Clerodendrum costatum R.Br) by the MODS (Microscopically Observed Drug Susceptibility) method, it was obtained that the three plants at concentrations of 250 ppm, 500 ppm, and 1000 ppm were only seen to have the growth of Micobacterium tuberculosis bacteria in the second week. Passive control with INH observation until week IV did not see any bacterial growth, while for negative control in the first week, the growth of Mycobacterium tuberculosis bacteria was seen. It can be concluded that a concentration greater than 1000 ppm is needed for the three plants to inhibit the growth of Mycobacterium tuberculosis bacteria.

Effects of growth space limitation on allometry of Ficus spp. In urban habitats

Tree morphological traits are related to the structural stability and their ability to provide ecological benefits such as carbon sequestration and water conservation. The allometric relationship can be used to construct the corresponding prediction equations in forests. However, the urban environment differs from the natural environment for trees. This study aims to enhance the urban tree growth model by classifying urban habitats. We achieved this by collecting morphological traits (DBH, height, crown depth, and width) commonly used in growth model researches of six Ficus spp. in Guangzhou, China, including Ficus virens, Ficus religiosa, Ficus elastica, Ficus benjamina, Ficus altissima, Ficus celebensis. We specifically examine how these trees grow in different urban settings. Urban habitats are classified based on growth space limitations, considering above-ground and underground. Results showed that certain morphologies in urban habitats did not conform to allometry (R2 < 0.4), and the model fitted with height as independent variable is better than that fitted with DBH. The model parameter following allometric relationship with tree height as the independent variable showed no significant difference, but the fit was worse in different urban habitats. On the contrary, models with DBH as the independent variable showed differences, for example, the allometric exponent of maximum crown width relative to DBH were 0.915 and 0.557 in two different habitats. Crown depth and tree height showed significant and stable allometry in the three habitats. The response of tree morphology to different habitats varied, and the allometric relationships among morphological traits showed differences in habitats where the growth space was limited. This underscores the considerable effect of the urban environment on urban tree growth models. Consequently, we recommend developing a series urban tree growth models tailored to different urban habitats to accurately quantify urban ecosystem services for assessing their sustainability in urban development and planning.

The natural food composition of long-tailed macaque (Macaca fascicularis Raffles, 1821) inhabiting the Muara Angke Wildlife Reserve in North Jakarta

Long-tailed macaque (Macaca fascicularis) is one species of wild animals inhabits Muara Angke Wildlife Reserve (SMMA), a wetland reserve predominantly grown by mangrove swamp forest. As there is high encounter of M. fascicularis with human and high incident of human-given food, it is important to understand the composition of natural food of M. fascicularis. The method used was quantitative with data collection techniques using scan sampling technique with 5-minute intervals. Natural food source for M. fascicularis in SMMA is provided by 17 species of plants. The main natural food sources providing both leaves and fruits are Ficus benjamina (54.62%) and Sonneratia caseolaris (28.9%). The most common plant parts eaten by M. fascicularis are leaves (61%) and fruit (21%), resembles to those of leaf monkeys (langurs). However, is a high possibility that nutrient needed to supplement high leaf consumption in SMMA monkeys is provided by non-natural human-given food.

Moraceae diversity in the Universitas Sumatera Utara’s arboretum

The arboretum of the Universitas Sumatera Utara (USU) is an area for collecting and conserving tree species in North Sumatra. One of dominant family found in USU Arboretum is Moraceae. The objective of this reserach was to determine Moraceae diversity in the USU Arboretum. The census approach of vegetation analysis was used to determine the identity and abundance of Moracea. The research found 9 (nine) Moraceae species belongs to two genera in the USU arboretum, those were Ficus microcarpa, Ficus elastic, Artocarpus heterophyllus, Ficus padana, Ficus fistulosa, Artocarpus altilis, Ficus benjamina, Ficus ampelas and Ficus hispida. The species with the highest density value (57.92%) is Ficus hispida. The diversity index, richness index, and species dominance index were all quite low in the research location, with values of 1.455, 1.226, and 0.370, respectively. Meanwhile, the species evenness index at the research location is 0.662, which is classified into moderate. To improve the diversity of Moraceae in the USU arboretum, species enrichment activities are needed.

First report of the ficus whitefly Singhiella simplex (Singh, 1931) (Hemiptera: Aleyrodidae) in Northern Italy and first observation of its association with the parasitoid wasp Encarsia hispida De Santis, 1948 (Hymenoptera: Aphelinidae) in Europe

AbstractIn February 2024, the ficus whitefly Singhiella simplex (Singh, 1931) (Hemiptera: Aleyrodidae) was first reported in a tropical greenhouse and inside a private house in Northern Italy (Vertemate con Minoprio and Monza, respectively – Lombardy Region). The observed ficus whiteflies caused severe defoliation on different Ficus species including Ficus benjamina and Ficus retusa. Several third and fourth larval instars of Singhiella simplex were associated with the parasitoid wasp Encarsia hispida De Santis, 1948 (Hymenoptera: Aphelinidae). Morphological and molecular analyses were performed to identify both S. simplex and E. hispida. Based on current knowledge, this is the first report of the ficus whitefly in Northern Italy as well as the first observation of its association with the parasitoid wasp E. hispida in Europe.

Intermediate pyrolysis of Ficus nitida wood in a fixed-bed reactor: effect of pyrolysis parameters on bio-oil and bio-char yields and properties

Intermediate pyrolysis of Ficus nitida wood in a fixed-bed reactor: effect of pyrolysis parameters on bio-oil and bio-char yields and properties

Impact of NPK and Cytokinin on Growth Characteristics and Chemical Composition of Ficus Nitida Plants

Abstract The research was conducted in a greenhouse at the Department of Horticulture and Garden Engineering, College of Agriculture, University of Anbar, Ramadi City, to study the impact of NPK supplementation and CPPU cytokinin spraying on the vegetative and root growth characteristics of Ficus nitida L. The study encompassed two primary factors. The first factor comprises the NPK chemical fertilizer in four distinct concentrations: F0 (no addition), F1 (1g urea, 3g DAB, 2g potassium sulfate), F2 (2g urea, 6g DAB, 4g potassium sulfate), and F3 (3g urea, 9g DAB, 4g potassium sulfate). The second factor involves the cytokine CPPU at three concentrations: C0 (no addition), C1 (3 mg L-1), and C2 (6 mg/liter). The study was designed using a randomized complete block design (RCBD) consisting of three replications, with three pots allocated to each experimental unit. Each replicate consisted of 36 treatments, meaning the experiment contained 108 experimental units in all blocks. The results indicated a significant increase in the interaction coefficients between the study factors in plant height, leaf area, nitrogen percentage in the leaf, total chlorophyll content, root surface area, and root length.. Specifically, these parameters were measured at 21.333 cm, 30.837 cm2, 2.666%, 1.384 g, 55.223 cm2, and 13.947 cm, respectively.

Anthracnose disease of Ficus benjamina caused by Colletotrichum orchidearum in Iran

Colletotrichum species cause the anthracnose disease that affects a wide variety of fruit, vegetable, and ornamental hosts. During the summer of 2018–2019, infected leaves of Ficus benjamina var. variegata with typical anthracnose symptoms were collected from several greenhouses in Miyandoab (West Azarbaijan Province) and Maraghe (East Azarbaijan Province) counties, Iran, with an incidence of 10–21%. The fungus was isolated and identified as Colletotrichum orchidearum, based on morphological features and multi-gene sequence analyses (ITS‒rDNA, GAPDH, CHS-1, HIS3, ACT and TUB2 genes). Pathogenicity assays were conducted on two-year-old potted plants with conidial suspensions under greenhouse conditions. Typical anthracnose symptoms including fungal conidiomata were developed on the inoculated leaves, while control plants remained symptomless. Re-isolation and re-identification of the inoculated fungi confirmed Koch’s postulates. This study provides the first evidence of C. orchidearum causing anthracnose disease on Ficus benjamina var. variegata in Iran and the world.

Bionic programmed wearable actuators based on 4D printing of liquid metal-spidroin-liquid crystal elastomer composite

ABSTRACT As a pivotal component of robotic systems and wearable devices, flexible actuators play a significant role; while it remains a challenge to achieve intelligent applications due to complex high-degree-of-freedom deformation control and insufficient functionality. Here, inspired by the leaf structure of Ficus benjamina 'Barok', a new type of programmable flexible actuator (PFA) prototype that can be 4D printed is designed, featuring a liquid metal-spidroin-liquid crystal elastomer composite printing functional structure and microstructure substrate, which achieves selective actuation and integrates sensing functions through differentiated manufacturing and pattern design. The interior of PFA is composed of functional masks with photothermal effects and differentiated photopolymerization mesogen structures. The microscopic rearrangement of asymmetric structures at different positions makes remote control of programming deformation convenient. Furthermore, benefiting from the integration of material properties, PFA can sense strain through resistive changes and connect to intelligent devices to transmit signals. The motion support and sensing performance of PFA have the potential to be applied to advanced robotics and human-machine interfaces.

Ethnoscience Study for Science Education Through The Process of Making Sopi Timor from Gewang Sap (Corypha utan Lamk.)

The Gewang plant (Corypha utan Lamk) is an endemic plant that grows in the dominant vegetation of Timor Tengah Selatan (South Central Timor Regency). Nira is obtained from the gewang tree (Corypha utan Lamk) by inserting the what flower stems and then stored in a container for further fermentation with a mixture of red oak (Saccharum officinarum Linn), the hanging roots of the chickpeas (Ficus benjamina), the stems of the schleichera (Schleichera oleosa), the stem of the beetle (Ziziphus mauritiana L.), and the forest stems. (Piper aduncum L.). This study aims to study and describe the process of making sopi timor and analyze the scientific concepts that exist in the process. Based on the results of the study of the production of this sopi timor, public knowledge of fermentation and separation of solutions can be used as one of the materials to teach science content in the classroom with a local cultural approach. In the relationship between ESD and a Merdeka curriculum, there is a similar pattern of teachers taking a role in studying science in a contextual way that can be sourced from local cultures. It is intended for students to engage in meaningful learning processes because the context of their learning is related to their daily lives. The results of this research can be used as recommendations in context-based chemistry, biology, and physics learning in accordance with the culture in the South Central Timor Regency.

Changes in endangered hornbill populations over space and time and potential ecological impacts in peninsular Thailand

Hornbills are particularly important seed dispersers in tropical Asian forests, but most of the species in the region are declining. In addition, long-term data on the extent of the declines and how forest degradation impacts hornbills remain mostly unquantified. Here, we used multi-covariate distance sampling to estimate the densities of hornbills in 2021–2022 in Hala-Bala Wildlife Sanctuary and Budo Su-Ngai Padi National Park protected areas in southern Thailand. These two sites are particularly useful for comparison due to differences in protection levels, with Bala being relatively less disturbed than Budo Mountain and because Bala was surveyed 20 years prior (2001–2002). Fig tree watches were also conducted in 2021–2022 to assess the potential impact of forest disturbance on hornbill visitation rates. Results indicated significant differences in hornbill densities between the two sites. The mean density of the Rhinoceros (Buceros rhinoceros) and Wreathed Hornbill (Rhyticeros undulatus) was 3.2 and 5.1 times higher in Bala than in Budo, while the mean density of the Great Hornbill (Buceros bicornis) was 2.2 times higher in Budo than in Bala. The density of the Helmeted Hornbill (Rhinoplax vigil) was notably lower than other hornbill species. There were also significant changes over the past 20 years in large hornbill densities, with Great Hornbill and Wreathed Hornbill significantly increasing, while the Helmeted Hornbill declined more than 90 %. Also, the probability of hornbills visiting fruiting Ficus trees was higher in the less-disturbed Bala than the Budo Mountain, making hornbills more reliable visitors in the less-disturbed site. We demonstrate the applicability of the multi-covariate distance sampling approach to estimate densities of rare hornbills, the negative impacts of habitat degradation on hornbills and the ecological role they play as seed dispersers. Additional surveys are needed throughout tropical Asia to establish further sites for long-term monitoring, especially in the Sunda region, where the Helmeted Hornbill is under extreme threat.