You have accessJournal of UrologyBenign Prostatic Hyperplasia: Evaluation & Markers1 Apr 2014PD25-10 CHARACTERIZATION OF FIBRILLAR COLLAGENS AND EXTRACELLULAR MATRIX OF GLANDULAR BENIGN PROSTATIC HYPERPLASIA NODULES Tyler Bauman, Tristan Nicholson, Brett Becker, Wei Huang, and William Ricke Tyler BaumanTyler Bauman More articles by this author , Tristan NicholsonTristan Nicholson More articles by this author , Brett BeckerBrett Becker More articles by this author , Wei HuangWei Huang More articles by this author , and William RickeWilliam Ricke More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.1989AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Recent studies have associated prostatic fibrosis with lower urinary tract symptoms (LUTS) independent of hyperplasia, indicating that fibrosis may be important in evaluating benign prostatic hyperplasia (BPH). However, collagen and extracellular matrix (ECM) composition within the normal and hyperplastic prostate have not previously been characterized. The purpose of this study was to evaluate collagen and ECM in normal prostate tissue and in glandular BPH, as well as the effects of therapies on these parameters. METHODS Fibrillar collagen and ECM were assessed in prostate tissues stained for picrosirius red (PSR) and Masson’s trichrome (MTC), respectively, using a tissue microarray (TMA) with glandular BPH tissue from patients with LUTS and normal prostate tissue from prostatectomy specimens. Additional tissues from BPH patients who underwent transurethral resection of the prostate (TURP) were assessed from patients treated with 5α-reductase inhibitors (5α-RIs) (n = 22) ± α-blockers (n = 38). Images were acquired and tissues were quantified in ImageJ. RESULTS Total collagen staining, quantified by birefringence of PSR-stained tissues viewed under circularly polarized light, was similar in normal prostate tissue (n = 48) and glandular BPH (n = 23) (125.9 ± 4.9 vs. 111.9 ± 5.1; p = 0.08). Differences of collagen bundle size were also assessed and no differences were observed. No differences were observed in the quantity of ECM in normal and BPH tissue quantified with MTC (12.97 ± 1.03 vs. 10.20 ± 1.24; p = 0.11). Collagen staining intensity was not associated with age (p = 0.63). In tissues from TURP specimens, treatment with 5α-RIs (p = 0.71) or α-blockers (p = 0.69) had no effect on total collagen staining (n = 47). Collagen staining was not associated with age (p = 0.61), pre-TURP prostate-specific antigen (p = 0.87), pre-TURP AUA symptom index (p = 0.11), or pre-TURP post-void residual (p = 0.67). CONCLUSIONS The association between fibrosis and LUTS that has been demonstrated in previous studies cannot be explained by increased fibrillar collagen or extracellular matrix deposition in glandular BPH nodules. If fibrosis is associated with BPH/LUTS, it is likely affecting collagen and ECM deposition within the periurethral tissue independent of nodular BPH. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e729 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Tyler Bauman More articles by this author Tristan Nicholson More articles by this author Brett Becker More articles by this author Wei Huang More articles by this author William Ricke More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...