The study evaluated the micro push-out bond strength of resin material (Multicore Flow) to two types of fiber posts (FP), namely fiber-reinforced composite (FRC) Postec and Radix Fiber posts using Er:YAG laser pretreatment. FP were divided into four groups, two being control groups. Before the core build-up procedure, representative specimens from each group were chosen to determine the surface roughness (Ra) at three different areas using a contact profilometer, while after the procedure, 1.5-mm-thick discs were sectioned and the micro push-out method was used to assess the bond strength of the core build-up material to the fiber post in each group. Two-way analysis of variance was used for statistical analysis with the level of significance set at p < 0.05. Scanning electron microscopy was used to analyze the post surfaces after Er:YAG laser pretreatment and to classify the failure mode after loading. The type of pretreatment (p < 0.001) and an interaction between the pretreatment and type of post (p < 0.001) had a significant effect on the bond strength, while the type of post did not (p = 0.965). The mean bond strength in the Er:YAG laser pretreatment group was significantly lower compared to the FRC Postec posts control group (p < 0.001), while there was no significant difference between the Radix Fiber posts groups (p = 0.680). Mean Ra values from the Er:YAG laser pretreatment groups were significantly higher compared to control groups (p < 0.001). Er:YAG laser pretreatment at tested parameters negatively affected the bond strength of Multicore to FP and cannot be recommended as a standard procedure.