Abstract We tested for dietary choices of foods varying in nutrient composition by cottontail rabbits on two college campuses in midwestern USA. We quantified choices among pellets of varying nutritional quality at artificial food patches. Dietary choices differed between seasons and locations. Spring giving-up densities (GUDs: food left behind) did not show differences in food choices and were lower than summer GUDs. In Appleton, the cottontails favoured both high protein and fibre pellets, whereas the medium protein and fibre pellets were favoured in Chicago. The cottontails maintained their choice of high protein, high fibre pellets at three spatial scales. The cottontails varied food intake to balance their protein, salt and fibre needs at different times and locations. Studying dietary choices and the effect of resource quality on foraging responses by urban wildlife provides a useful tool to study ecological interactions and can help minimize damage in urban environments such as parks.