Fiber Cells Research Articles

ATF4 May Be Essential for Adaption of the Ocular Lens to Its Avascular Environment.

The late embryonic mouse lens requires the transcription factor ATF4 for its survival although the underlying mechanisms were unknown. Here, RNAseq analysis revealed that E16.5 Atf4 null mouse lenses downregulate the mRNA levels of lens epithelial markers as well as known markers of late lens fiber cell differentiation. However, a comparison of this list of differentially expressed genes (DEGs) with other known transcriptional regulators of lens development indicated that ATF4 expression is not directly controlled by the previously described lens gene regulatory network. Pathway analysis revealed that the Atf4 DEG list was enriched in numerous genes involved in nutrient transport, amino acid biosynthesis, and tRNA charging. These changes in gene expression likely result in the observed reductions in lens free amino acid and glutathione levels, which would result in the observed low levels of extractable lens protein, finally leading to perinatal lens disintegration. These data demonstrate that ATF4, via its function in the integrated stress response, is likely to play a crucial role in mediating the adaption of the lens to the avascularity needed to maintain lens transparency.

Experimental studies of dynamic compression of cellulose pulp fibers

The ability to control the structure of the wood-pulp fiber cell wall is an attractive means to obtain increased accessibility to the fiber interior, providing routes for functionalization of the fibers that support further processing and novel material concepts, e.g. improved degree of polymerization, nanofiltration as demonstrated in previous studies. It has been proposed that dynamic compression and decompression of the cellulose pulp fibers in the wet state make it possible to modify the cell wall significantly.We hypothesize that hydrostatic pressure exerted on fibers fully submerged in water will increase the accessibility of the fiber wall by penetrating the fiber through weak spots in the cell wall. To pursue this, we have developed an experimental facility that can subject wet cellulose pulp samples to a pressure pulse ∼10 ms long and with a peak pressure of ∼300 MPa. The experiment is thus specifically designed to elucidate the effect of a rapid high-pressure pulse passing through the cellulose sample and enables studies of changes in structural properties over different size ranges. Different characterization techniques, including Scanning electron microscopy, X-ray diffraction, and wide- and small-angle X-ray scattering, have been used to evaluate the material exposed to pulsed pressure. The mechanism of pressure build-up is estimated computationally to complement the results. Key findings from the experiments consider a decrease in crystallinity and changes in the surface morphology of the cellulose sample.

Systematically and Comprehensively Understanding the Regulation of Cotton Fiber Initiation: A Review.

Cotton fibers provide an important source of raw materials for the textile industry worldwide. Cotton fiber is a kind of single cell that differentiates from the epidermis of the ovule and provides a perfect research model for the differentiation and elongation of plant cells. Cotton fiber initiation is the first stage throughout the entire developmental process. The number of fiber cell initials on the seed ovule epidermis decides the final fiber yield. Thus, it is of great significance to clarify the mechanism underlying cotton fiber initiation. Fiber cell initiation is controlled by complex and interrelated regulatory networks. Plant phytohormones, transcription factors, sugar signals, small signal molecules, functional genes, non-coding RNAs, and histone modification play important roles during this process. Here, we not only summarize the different kinds of factors involved in fiber cell initiation but also discuss the mechanisms of these factors that act together to regulate cotton fiber initiation. Our aim is to synthesize a systematic and comprehensive review of different factors during fiber initiation that will provide the basics for further illustrating these mechanisms and offer theoretical guidance for improving fiber yield in future molecular breeding work.

Waterlogging restricted cotton fiber elongation by reducing osmolyte accumulation and cell wall biosynthesis

Waterlogging severely threatens the formation of cotton fiber quality, especially the length. However, the underlying mechanism is still incompletely understood. Here, the experiment (control: soil relative water content at (75 ± 5)%; treatment: waterlogging for 6 days) was conducted during the flowering and boll forming stage using two upland cotton cultivars (Dexiamian 1 and Yuzaomian 9110) to investigate the effects of waterlogging on fiber development from both transcriptomic and physiological levels. The transcriptome analysis of 10 days post anthesis cotton fiber showed that waterlogging significantly regulated the expression of genes associated with fiber elongation events including osmotic solutes accumulation, fiber cell wall biosynthesis, and cell wall remodeling and loosening. Waterlogging decreased the sucrose input into cotton fiber, resulting in reduced sucrose and hexose contents. Under waterlogging, the accumulation of malate and K+ in both cultivars was reduced by decreasing phosphoenolpyruvate carboxylase (PEPC) activity and potassium transporter genes (GhPOT) expression, respectively. Thus, the total concentration of osmolytes was reduced, which dampened cotton fiber elongation. Due to reduced sucrose synthase activity and uridine diphosphate glucose content, the cellulose biosynthesis was limited, leading to decreased fiber cell wall accumulation, which was adverse to fiber elongation. Besides, genes responsible for cell wall remodeling and loosening, including xyloglucan endotransglucosylase/hydrolase genes (GhXTH), α-expansin genes (GhEXPA), and pectin methylesterase genes (GhPME) were also responsive to waterlogging, but in different ways between cultivars. Overall, the shortened cotton fiber could be attributed to reduced osmolytes accumulation and cell wall biosynthesis, whilst the effects of waterlogging on cotton fiber cell wall loosening require further exploration.

Comprehensive landscape of RNA N6-methyladenosine modification in lens epithelial cells from normal and diabetic cataract

To gain more insight into the mechanism of cataract formation from the perspective of epigenetics in the diabetic population, lens epithelium from diabetic cataract patients and healthy donors were collected separately and analyzed for N6-methyladenosine (m6A)-modified RNA using methylated RNA immunoprecipitation sequencing (MeRIP-Seq). Subsequently, differential expression analysis was performed on m6A-regulated messenger RNA (mRNA), circular RNA (circRNA), and long non-coding RNA (lncRNA), followed by functional annotation using the Gene Ontology (GO) database. Furthermore, analysis of single-cell data of lens complemented the intrinsic association and cellular heterogeneity of cataract and m6A regulators. In this study, both the global expression levels and peak intensity of m6A-tagged RNAs were increased in patients with diabetic cataract. And we noted multiple core enzymes were upregulated in the diabetic cataract (DC) samples. Besides, single-cell RNA sequencing analysis of the lens revealed the heterogeneous expression of RNA m6A regulators across different cell types, and we noted that the early fiber cell cluster was also closely associated with the onset of cataract and m6A modification. The results comprehensively revealed the dynamic modification landscape of m6A on mRNA, circRNA, and lncRNA, which might provide valuable resources for future studies of the pathogenesis of DCs.

Topical Nerve Growth Factor (NGF) restores electrophysiological alterations in the Ins2Akita mouse model of diabetic retinopathy

People suffering from diabetes mellitus commonly have to face diabetic retinopathy (DR), an eye disease characterized by early retinal neurodegeneration and microvascular damage, progressively leading to sight loss. The Ins2Akita (Akita) diabetic mouse presents the characteristics of DR and experimental drugs can be tested on this model to check their efficacy before going to the clinic. Topical administration of Nerve Growth Factor (NGF) has been recently demonstrated to prevent DR in the Akita mouse, reverting the thinning of retinal layers and protecting the retinal ganglion cells (RGCs) from death. In this study, we characterize the effects of topical NGF on neuroretina function, quantified with the electroretinogram (ERG). In particular, we show that NGF can ameliorate RGC conduction in the retina of Akita mice, which correlates with a recovery of retinal nerve fiber plus ganglion cell layer (RNFL-GCL) structure. Overall, our preclinical results highlight that topical administration of NGF could be a promising therapeutic approach for DR, being capable of exerting a beneficial impact on retinal functionality.

Betholletia excelsa Fruit: Unveiling Toughening Mechanisms and Biomimetic Potential for Advanced Materials.

Dry fruits and nutshells are biological capsules of outstanding toughness and strength with biomimetic potential to boost fiber-reinforced composites and protective structures. The strategies behind the Betholletia excelsa fruit mechanical performance were investigated with C-ring and compression tests. This last test was monitored with shearography and simulated with a finite element model. Microtomography and digital and scanning electron microscopy evaluated crack development. The fruit geometry, the preferential orientation of fibers involved in foam-like sclereid cells, promoted anisotropic properties but efficient energy dissipating mechanisms in different directions. For instance, the mesocarp cut parallel to its latitudinal section sustained higher forces (26.0 ± 2.8 kN) and showed higher deformation and slower crack propagation. The main toughening mechanisms are fiber deflection and fiber bridging and pullout, observed when fiber bundles are orthogonal to the crack path. Additionally, the debonding of fiber bundles oriented parallel to the crack path and intercellular cracks through sclereid and fiber cells created a tortuous path.

Extraction and characterization of novel natural cellulosic fiber from Echinops spinosissimus plant stem

Bio-based composites are outstanding materials to replace many synthetic fiber-based composites due to their biodegradability and sustainable environmental properties. This research aims to characterize and analysis a new cellulosic fibers extracted from Echinops spinosissimus plant stem, to study their integration in the field of various industries such as composites and textiles. Thus, adding and incorporating a new natural fiber to the list of fibers that enter into the production of Bio-based composites materials, and meet the needs of the market that knows a shortage of these materials. In addition, the extracted fibers from Echinops spinosissimus plant, which is abundant and have acceptable properties that can replace the synthetic fibers. The stem anatomy results showed a strong presence of fiber cells, fiber SEM micrographs showed that fiber surface become rough, these fibers have a density of 0.97 ± 0.01 g/cm3, and diameter 280.76 µm. FTIR and XRD showed that these fibers are cellulosic. Thermal analysis revealed that Echinops spinosissimus fiber have a thermal stability of 208°C. Tensile strength, Young modulus and strain at failure were found as 217.54 ± 54.16 MPa, 19.23 ± 0.76 GPa and 3.78 ± 0.36%, respectively. SEM showed that the studied fiber becomes more roughness in the outer surface following the chemical treatments, thus contributing to better adhesion with resin. This work confirmed that Echinops spinosissimus fibers are suitable materials for use in textile and biocomposite applications.

The involvement of caspases in the process of nuclear removal during lens fiber cell differentiation

The terminal differentiation of lens fiber cells involves elimination of their organelles, which must occur while still maintaining their functionality throughout a lifetime. Removal of non-nuclear organelles is accomplished through induction of autophagy following the spatiotemporal suppression of the PI3K/Akt signaling axis. However, blocking this pathway is not alone sufficient to induce removal of fiber cell nuclei. While the final steps in fiber cell nuclear elimination are highlighted by the appearance of TUNEL-positive nuclei, which are associated with activation of the lens-specific DNaseIIβ, there are many steps in the process that precede the appearance of double stranded DNA breaks. We showed that this carefully regulated process, including the early changes in nuclear morphology resulting in nuclear condensation, cleavage of lamin B, and labeling by pH2AX, is reminiscent of the apoptotic process associated with caspase activation. Multiple caspases are known to be expressed and activated during lens cell differentiation. In this study, we investigated the link between two caspase downstream targets associated with apoptosis, ICAD, whose cleavage by caspase-3 leads to activation of CAD, a DNase that can create both single- and double-stranded DNA cleavages, and lamin B, a primary component of the nuclear lamina. We discovered that the specific inhibition of caspase-3 activation prevents both lamin B and DNA cleavage. Inhibiting caspase-3 did not prevent nuclear condensation or removal of the nuclear membrane. In contrast, a pan-caspase inhibitor effectively suppressed condensation of fiber cell nuclei during differentiation. These studies provide evidence that caspases play an important role in the process of removing fiber cell nuclei during lens differentiation.

Synergistic effect of FGF-2 and TGF-β1 on the mineralization of human umbilical cord perivascular cells

ObjectiveHuman umbilical cord perivascular cells (HUCPVCs) are derived from the human umbilical cord perivascular tissue and are expected to replace mesenchymal stromal cells in the future. We investigated the synergistic effects of fibroblast growth factor 2 (FGF-2) and transforming growth factor-beta 1 (TGF-β1) on HUCPVC mineralization. DesignWe prepared HUCPVCs with (FGF(+)HUCPVCs) or without FGF-2 (FGF(-)HUCPVCs) in the presence of activated vitamin D3, a bone morphogenic protein inhibitor, and TGF-β1. We examined the cell proliferative capacity, expression of various hard tissue-forming cell gene markers, and mineralization induction ability and identified the crystalline phases of the mineralized nodules. ResultsFGF(+)HUCPVCs exhibited higher intracellular alkaline phosphatase (ALP) gene expression and ALP activity, and their cell proliferation rate was higher than that of FGF(-)HUCPVCs. The expression levels of osteoblast marker genes increased in FGF(+)HUCPVCs, whereas those of elastic fiber and muscle cell markers increased in FGF(-)HUCPVCs. The expression of genes related to matrix vesicle-mediated mineralization was increased in FGF(+)HUCPVCs. While FGF(-)HUCPVCs displayed myofibroblast-like properties and could not induce mineralization, FGF(+)HUCPVCs demonstrated the ability to produce mineralized nodules. The resulting mineralized nodules consisted of hydroxyapatite as the major phase and minor amounts of octacalcium phosphate. The mineralized nodules exhibited the morphological characteristics of bone hydroxyapatite, composed of fibrous hydroxyapatite nanorods and polycrystalline sheets. ConclusionWe found that FGF-2 synergizes with TGF-β1 and is a key factor in the differentiation of HUCPVCs into osteoblast-like cells. Thus, HUCPVCs can potentially serve as a new stem cell source for future bone regeneration and dental treatments.

Activation of Gossypium hirsutum ACS6 Facilitates Fiber Development by Improving Sucrose Metabolism and Transport.

Cotton fiber yield depends on the density of fiber cell initials that form on the ovule epidermis. Fiber initiation is triggered by MYB-MIXTA-like transcription factors (GhMMLs) and requires a sucrose supply. Ethylene or its precursor ACC (1-aminocyclopropane-1-carboxylic acid) is suggested to affect fiber yield. The Gossypium hirsutum (L.) genome contains 35 ACS genes (GhACS) encoding ACC synthases. Here, we explored the role of a GhACS family member in the regulation of fiber initiation. Expression analyses showed that the GhACS6.3 gene pair was specifically expressed in the ovules during fiber initiation (3 days before anthesis to 5 days post anthesis, -3 to 5 DPA), especially at -3 DPA, whereas other GhACS genes were expressed at very low or undetectable levels. The expression profile of GhACS6.3 during fiber initial development was confirmed by qRT-PCR analysis. Transgenic lines overexpressing GhACS6.3 (GhACS6.3-OE) showed increased ACC accumulation in ovules, which promoted the formation of fiber initials and fiber yield components. This was accompanied by increased transcript levels of GhMML3 and increased transcript levels of genes encoding sucrose transporters and sucrose synthase. These findings imply that GhACS6.3 activation is required for fiber initial development. Our results lay the foundation for further research on increasing cotton fiber production.

Quantitative examination of the anatomy of the juvenile sugar maple xylem.

New methodologies have enabled viable sap yields from juvenile sugar maple trees. To further improve yields, a better understanding of sap exudation is required. To achieve this, the anatomy of the xylem must first be fully characterised. We examine juvenile maple saplings using light optical microscopy (LOM) and scanning electron microscopy (SEM), looking at sections cut along differing orientations as well as macerations. From this we measure various cell parameters. We find diameter and length of vessel elements to be 28 ± 8 μm and 200 ± 50 μm, for fibre cells 8 ± 3 μm and 400 ± 100 μm, and for ray parenchyma cells 8 ± 2 μm and 50 ± 20 μm. We also examine pitting present on different cell types. On vessel elements we observe elliptical bordered pits connecting to other vessel elements (with major axis of 2.1 ± 0.7 μm and minor 1.3 ± 0.3 μm) and pits connecting to ray parenchyma (with major axis of 4 ± 2 μm and minor 2.0 ± 0.7 μm). We observe two distinct pit sizes on fibres with circular pits 0.7 ± 0.2 μm in diameter and ellipsoidal pits 1.6 ± 0.4 μm by 1.0 ± 0.3 μm. We do not observe distinct pitting patterns on different fibre types. The various cell and pit measurements obtained generally agree with the limited data available for mature trees, with the exception of vessel element and fibre length, both of which were significantly smaller than reported values.

Effect of sisal fibre treatment on the hydration and thermophysical properties of a cement biocomposite

Cement-based biocomposites are a current area of construction research. Plant fibres retard the setting times of cement due to their hydrophilic nature and the thermal properties of raw cement mortar containing plant fibres can be problematic. This aim of this work was to study the effect of fibre treatment to reduce the hydrophilicity of natural fibres while enhancing the thermal properties of the cementitious matrix. The first part of this study focused on structural and morphological aspects. Scanning electron microscopy showed a tiny calcium layer around the sisal fibre treated with sodium hydroxide and paraffin oil on the adhesion surface. Fourier transform infrared spectroscopy revealed a disparity in the peaks of the absorption bands of calcium carbonate and calcium hydroxide. The treatment slowed the alkaline hydrolysis and mineralisation of the fibre cell walls in the interfacial transition zone, thus promoting cement hydration. The second part of this work was a thermophysical study of the composites. The inclusion of sisal fibres led to remarkable decreases in thermal conductivity and heat capacity. The best thermal properties were obtained for the composite with sisal fibre immersed in paraffin oil. Biomortars with treated sisal fibre are thus promising from an insulation point of view.

The formation of xylem tissues and secondary cell walls is diminished by severe and consecutive insect defoliation.

Insect defoliation causes unusual wood structures in forest trees. However, the characteristics of the unusual wood structure of hardwoods remain unclear. The aim of this study was to clarify the characteristics of the wood structure in trunks produced under severe insect defoliation in Betula maximowicziana trees. Samples from the trunks were taken from two sites (Naie and Furano) in central Hokkaido where insect defoliation by Caligula japonica occurred during 2006-2012. The cross-dating and microscopic observations with histochemical staining were done. White rings with thin-walled wood fibers and drastic reduction in annual ring width in the subsequent year were observed in both samples. From these results, the formation year of white rings were determined and severe defoliation was confirmed to trigger white ring formation. The characteristics may prove useful for the detection of the formation year of white rings. Then, we clarified the cell morphology of wood fibers in the white rings. Scanning electron microscopy and histochemical analyses indicated that the thickness of the S2 layer of wood fibers decreased, but xylan and lignin were still deposited in the cell walls of wood fibers in a white ring. However, the cell-wall thickness of wood fibers recovered after defoliation period. Our results suggest that B. maximowicziana would respond to a temporary lack of carbon due to insect defoliation by regulating the thickness of the wood-fiber S2 layer. For B. maximowicziana, insect defoliation during the late growing season has serious deleterious effects on wood formation and radial growth. This article is protected by copyright. All rights reserved.

GhSMO2-2 is regulated by brassinosteroid signal and involved in cotton fiber elongation via influencing phytosterol and sphingolipid biosynthesis

Cotton fiber cell is an extremely elongated single cell and is regarded as an ideal material to study the growth and development of plant cell. Phytohormone brassinosteroids (BRs) play crucial roles in fiber cell development. However, its action mechanism in fiber growth is largely unknown. In this study, we identified a homologue of Arabidopsis SMO2–2 (Sterol C-4α Methyl Oxidase), GhSMO2–2, in upland cotton (Gossypium hirsutum L.), which predominantly expressed in fiber cell and peaked at the rapid elongation stage, and responded to BR signal. The GhBES1, a transcription factor in BR signaling could directly bind to the promoter of GhSMO2–2 and promoted its expression. Overexpression of GhSMO2–2 significantly promoted fiber cell elongation and phytosterol synthesis in cotton, and elevated some sphingolipid species that was important for fiber elongation. On the contrary, down-regulating GhSMO2–2 suppressed fiber elongation and blocked these chemicals accumulation. These results indicated that GhSMO2–2 is an important gene involved in fiber cell elongation, and is valuable for genetic engineering to improve fiber quality and yield. Taken together, our study revealed that BR regulated fiber cell elongation by influencing phytosterol and sphingolipid biosynthesis, the key components of membrane lipid raft and provided a new clue for further revealing the function and mechanism of phytosterols, sphingolipids, and BRs in plant cell development.

Multiomic analysis implicates FOXO4 in genetic regulation of chick lens fiber cell differentiation

A classic model for identification of novel differentiation mechanisms and pathways is the eye lens that consists of a monolayer of quiescent epithelial cells that are the progenitors of a core of mature fully differentiated fiber cells. The differentiation of lens epithelial cells into fiber cells follows a coordinated program involving cell cycle exit, expression of key structural proteins and the hallmark elimination of organelles to achieve transparency. Although multiple mechanisms and pathways have been identified to play key roles in lens differentiation, the entirety of mechanisms governing lens differentiation remain to be discovered. A previous study established that specific chromatin accessibility changes were directly associated with the expression of essential lens fiber cell genes, suggesting that the activity of transcription factors needed for expression of these genes could be regulated through binding access to the identified chromatin regions. Sequence analysis of the identified chromatin accessible regions revealed enhanced representation of the binding sequence for the transcription factor FOXO4 suggesting a direct role for FOXO4 in expression of these genes. FOXO4 is known to regulate a variety of cellular processes including cellular response to metabolic and oxidative stress, cell cycle withdrawal, and homeostasis, suggesting a previously unidentified role for FOXO4 in the regulation of lens cell differentiation. To further evaluate the role of FOXO4 we employed a multiomics approach to analyze the relationship between genome-wide FOXO4 binding, the differentiation-specific expression of key genes, and chromatin accessibility. To better identify active promoters and enhancers we also examined histone modification through analysis of H3K27ac. Specific methods included CUT&RUN (FOXO4 binding and H3K27ac modification), RNA-seq (differentiation state specific gene expression), and ATAC-seq (chromatin accessibility). CUT&RUN identified 20,966 FOXO4 binding sites and 33,921 H3K27ac marked regions across the lens fiber cell genome. RNA-seq identified 956 genes with significantly greater expression levels in fiber cells compared to epithelial cells (log2FC > 0.7, q < 0.05) and 2548 genes with significantly lower expression levels (log2FC < −0.7, q < 0.05). Integrated analysis identified 1727 differentiation-state specific genes that were nearest neighbors to at least one FOXO4 binding site, including genes encoding lens gap junctions (GJA1, GJA3), lens structural proteins (BFSP1, CRYBB1, ASL1), and genes required for lens transparency (HSF4, NRCAM). Multiomics analysis comparing the identified FOXO4 binding sites in published ATAC-seq data revealed that chromatin accessibility was associated with FOXO4-dependent gene expression during lens differentiation. The results provide evidence for an important requirement for FOXO4 in the regulated expression of key genes required for lens differentiation and link epigenetic regulation of chromatin accessibility and H3K27ac histone modification with the function of FOXO4 in controlling lens gene expression during lens fiber cell differentiation.

Investigating the influence of plant fiber geometry on apparent transverse elastic properties through finite element analysis

This study explores the link between plant fiber geometry and its transverse behavior, focusing on the resulting apparent transverse stiffness as determined by analytical models. Using Finite Element Analysis (FEA) plant fiber transverse compression is simulated with two-dimensional models. Simplified geometric representations of common geometric features are examined, showing how distinct attributes influence behavior. The fiber lumen is shown to decrease apparent stiffness, while elliptical geometry and flat sections increase it. An adaptation of analytical models for elliptical cross-sections yields a 93 % improvement on identification accuracy. Furthermore, the transverse compression of realistic geometries, extracted from microscopy images and presenting a blend of different features, is simulated. The lumen’s impact on apparent stiffness is shown to outweigh the effects of other features. These findings show the importance of apparent stiffness over fiber cell wall stiffness and how it may evolve under repeated loading, which has important implications for the composites sector.

The activation of the NAC transcription factor by Poplar PtrGATA9 facilitates the secondary cell wall deposition of interfascicular fiber cell in Arabidopsis

The activation of the NAC transcription factor by Poplar PtrGATA9 facilitates the secondary cell wall deposition of interfascicular fiber cell in Arabidopsis

Binding of βL-Crystallin with Models of Animal and Human Eye Lens-Lipid Membrane.

Several discoveries show that with age and cataract formation, β-crystallin binds with the lens membrane or associates with other lens proteins, which bind with the fiber cell plasma membrane, accompanied by light scattering and cataract formation. However, how lipids (phospholipids and sphingolipids) and cholesterol (Chol) influence β-crystallin binding to the membrane is unclear. This research aims to elucidate the role of lipids and Chol in the binding of β-crystallin to the membrane and the membrane's physical properties (mobility, order, and hydrophobicity) with β-crystallin binding. We used electron paramagnetic resonance (EPR) spin-labeling methods to investigate the binding of βL-crystallin with a model of porcine lens-lipid (MPLL), model of mouse lens-lipid (MMLL), and model of human lens-lipid (MHLL) membrane with and without Chol. Our results show that βL-crystallin binds with all of the investigated membranes in a saturation manner, and the maximum parentage of the membrane surface occupied (MMSO) by βL-crystallin and the binding affinity (Ka) of βL-crystallin to the membranes followed trends: MMSO (MPLL) > MMSO (MMLL) > MMSO (MHLL) and Ka (MHLL) > Ka (MMLL) ≈ Ka (MPLL), respectively, in which the presence of Chol reduces the MMSO and Ka for all membranes. The mobility near the headgroup regions of the membranes decreases with an increase in the binding of βL-crystallin; however, the decrease is more pronounced in the MPLL and MMLL membranes than the MHLL membrane. In the MPLL and MMLL membranes, the membranes become slightly ordered near the headgroup with an increase in βL-crystallin binding compared to the MHLL membrane. The hydrophobicity near the headgroup region of the membrane increases with βL-crystallin binding; however, the increase is more pronounced in the MPLL and MMLL membranes than the MHLL membrane, indicating that βL-crystallin binding creates a hydrophobic barrier for the passage of polar molecules, which supports the barrier hypothesis in cataract formation. However, in the presence of Chol, there is no significant increase in hydrophobicity with βL-crystallin binding, suggesting that Chol prevents the formation of a hydrophobic barrier, possibly protecting against cataract formation.

The effect of fibre cell remodelling on the power and optical quality of the lens.

Vertebrate eye lenses are uniquely adapted to form a refractive index gradient (GRIN) for improved acuity, and to grow slowly in size despite constant cell proliferation. The mechanisms behind these adaptations remain poorly understood. We hypothesize that cell compaction contributes to both. To test this notion, we examined the relationship between lens size and shape, refractive characteristics and the cross-sectional areas of constituent fibre cells in mice of different ages. We developed a block-face imaging method to visualize cellular cross sections and found that the cross-sectional areas of fibre cells rose and then decreased over time, with the most significant reduction occurring in denucleating cells in the adult lens cortex, followed by cells in the embryonic nucleus. These findings help reconcile differences between the predictions of lens growth models and empirical data. Biomechanical simulations suggested that compressive forces generated from continuous deposition of fibre cells could contribute to cellular compaction. However, optical measurements revealed that the GRIN did not mirror the pattern of cellular compaction, implying that compaction alone cannot account for GRIN formation and that additional mechanisms are likely to be involved.