In this work, an in-fiber Fabry-Perot interferometer (FPI) is proposed and experimentally demonstrated for ambient relative humidity (RH) and temperature measurements. The FPI is manufactured from short segments of hollow-core fiber (HCF) and photonic crystal fiber (PCF). The air-cavity in the HCF segment enables a strong light-air interaction whereas the air holes of the PCF serve as vents which enable the exchange of air between the air-cavity and the surrounding medium. Our findings show that the dip intensity is solely sensitive to the change in RH with a linear sensitivity of 0.06273 dB/%RH in the range of 35–71%RH whereas the dip wavelength is solely sensitive to the ambient temperature with a linear sensitivity of 10.64 pm/°C in the range of 25–70 °C. The cross-sensitivity between the two measurands can be disregarded, therefore no sophisticated equation is required for the discriminative measurement of humidity and temperature.