The effects of curing conditions on the mechanical properties of glass fiber reinforced epoxy composites under different fiber prestressing levels were quantitatively studied. The composite samples were prepared with silica particle modified continuous E-glass fibers, epoxy resin matrix, fiber prestressing and step curing processing. Room temperature cured and isothermal high temperature cured composite samples, with the same content and structure, were made to study the difference of three curing conditions on a quantitative level. Based on the test results of flexural, shear, and impact properties of the composite samples, it was found that the step curing procedure generates composite samples with up to 47% and 14% increased properties than room temperature cured and isothermal high temperature cured samples, respectively, when manufactured under the same fiber prestressing level. The possibility of generating an optimum residual stress within the samples during processing is proposed and discussed to explain the contribution of the step curing process to the composite properties.