To effectively assess and manage aging infrastructure, sensing technologies that produce accurate and useful quantitative data are required. Distributed fiber optic strain measurement systems are one potential technology that could fulfill this requirement. This case study investigates the performance of a distributed fiber optic strain measurement technology with high accuracy and spatial resolution during a load test on a RC bridge. The measurements from the fiber optic system are compared with conventional strain gauges and linear transducers, and good agreement between the sensors was found. The fiber optic measurements are also used to examine the bridge support conditions as well as the load distribution between beams. The results show that assumptions made about the support conditions during design do not match the actual bridge behavior, although the load distribution between beams is as expected.