ABSTRACT Natural fibers are being investigated as alternatives to synthetic fibers in composite materials. In this paper, the physical, chemical, thermal, and morphological characteristics of fibers derived from milk thistle flower heads were characterized as potential polymer reinforcement material using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM). The fibers were found to contain 42.9% cellulose, 21.2% hemicellulose, and a negligible lignin content. The fiber constituent functional groups were similar to those observed for other natural fibers. The crystallite size and the crystallinity index were found to be 2.23 nm and 84 ± 3%, respectively. The thermal stability of the fibers was found to be 220°C. The fibers start rapidly degrading above 290°C. Morphologically, the fibers appeared to have a nonporous surface with well-structured longitudinal parallel grooves. Cross sectionally, the fibers consisted of multiple hollow lumens interconnected with cell walls. The hollowed nature of the fibers explains the low value (0.33 ± 0.04 g mL−1) of their density. The tensile strength of the fibers was determined to be 22 ± 1 MPa. In conclusion, the fibers of the flower heads of milk thistle can be used as effective reinforcements in polymer matrices.