Fiber Bragg grating (FBG) sensor has a lot of advantages over the resistance strain gauge and has been used in many applications. However, there are few applications of rotor blade dynamic measurement in helicopter flight. In this paper, a method for blade dynamic strain measurement using an FBG sensor in a helicopter's real flight is presented. The corresponding measurement system is established and can eliminate the effects of the helicopter's electromagnetic environment on the electrical sensing components in the measurement system with the orthogonal frequency-division multiplexing modulation. The measured dynamic strains on the rotor blades of the helicopter in real flight contain six harmonic frequencies with the vibration characteristics of rotor blades, indicating that the established FBG measurement method and system have practical engineering applications.