Few Hours Of Infection Research Articles

Nuclear matrix modifications at different stages of infection by herpes simplex virus type 1.

In BHK-21 cells infected with herpes simplex virus type 1 many virus-induced proteins were found attached to the nuclear matrix. To understand the role of this cell fraction during virogenesis, matrix-associated proteins were analysed at different stages of infection. All the immediate-early protein species were bound to the nuclear matrix and their association with this structure was stable. During the first few hours of infection, the pattern of virus-induced proteins attached to the nuclear matrix remained identical, indicating that polypeptides from the early group are not associated with this cell fraction. Among the late proteins, which are generally structural proteins, 60% of the nuclear proteins were tightly bound to the nuclear matrix. This suggests that the nuclear matrix is involved in at least two different events during virogenesis, regulation of viral infection and assembly of viral capsids.

LPP-1 infection of the blue-green alga Plectonema boryanum. II. Viral deoxyribonucleic acid synthesis and host deoxyribonucleic acid breakdown.

Host and viral deoxyribonucleic acid (DNA) metabolism in LPP-1-infected Plectonema boryanum was studied by equilibrium centrifugation in CsCl gradients. Approximately 50% of the host DNA is degraded to acid-soluble material between 3 and 7 hr after infection. Most of the acid-soluble product is reincorporated into viral DNA. Incorporation of exogenous (3)H-adenine into viral DNA can be detected very early after infection (within the first 2 hr), but the bulk of viral DNA synthesis occurs between 6 and 8 hr. Both the breakdown of host DNA and the synthesis of viral DNA require protein synthesis during the first few hours of infection.

Energy metabolism of brain homogenates from mice infected with GD VII virus

Respiration and phosphorylation were measured in brain homogenates from mice that had been infected with Theiler's GD VII virus. All of the expected respiratory carriers were present in the brain preparations; the mitochondrial fraction was 0.18 μM in cytochrome a + a 3 and about the same for the other respiratory carriers. There was 20 times as much pyridine nucleotide as a + a 3. No alteration in the ADP O ratio was detected following virus infection. The oxidation of succinate was inhibited by 40–45% in virus-infected preparations while oxidation of the NAD + linked substrate, α-ketoglutarate, was elevated. β-Hydroxybutyrate oxidation was unchanged. The change in succinate oxidation occurred during the first few hours of infection and coincided with virus production.