Feverfew Research Articles

Regioselective and Stereoselective Synthesis of Parthenolide Analogs by Acyl Nitroso-Ene Reaction and Their Biological Evaluation against Mycobacterium tuberculosis.

Historically, natural products have played a major role in the development of antibiotics. Their complex chemical structures and high polarity give them advantages in the drug discovery process. In the broad range of natural products, sesquiterpene lactones are interesting compounds because of their diverse biological activities, their high-polarity, and sp3-carbon-rich chemical structures. Parthenolide (PTL) is a natural compound isolated from Tanacetum parthenium, of the family of germacranolide-type sesquiterpene lactones. In recent years, parthenolide has been studied for its anti-inflammatory, antimigraine, and anticancer properties. Recently, PTL has shown antibacterial activities, especially against Gram-positive bacteria. However, few studies are available on the potential antitubercular activities of parthenolide and its analogs. It has been demonstrated that parthenolide's biological effects are linked to the reactivity of α-exo-methylene-γ-butyrolactone, which reacts with cysteine in targeted proteins via a Michael addition. In this work, we describe the ene reaction of acylnitroso intermediates with parthenolide leading to the regioselective and stereoselective synthesis of new derivatives and their biological evaluation. The addition of hydroxycarbamates and hydroxyureas led to original analogs with higher polarity and solubility than parthenolide. Through this synthetic route, the Michael acceptor motif was preserved and is thus believed to be involved in the selective activity against Mycobacterium tuberculosis.

Role of parthenolide in paclitaxel-induced oxidative stress injury and impaired reproductive function in rat testicular tissue

The chemotherapeutic agent paclitaxel (PTX) causes testicular toxicity due to oxidative stress. Parthenolide (PTL), the active ingredient of the Tanacetum parthenium plant, is used to treat inflammation, dizziness, and spasms. In the present study, we evaluated the therapeutic effect of PTL on PTX-induced testicular toxicity in rats and its role in reproductive function. To this end, 6 groups were formed: control, PTX, sham, T1, T2, and T3. After testicular toxicity was induced in rats with 8 mg/kg PTX, the rats were treated with 1 mg/kg, 2 mg/kg, and 4 mg/kg PTL for 14 days. GSH and MDA levels were measured in rat testicular tissue after the last dose of PTL was administered. To determine the damage caused by PTX to testicular tissue by detecting 8-OHdG and iNOS, sections were prepared and examined histopathologically and immunohistochemically. Furthermore, the gene expressions and enzymatic activities of SOD, CAT, GPx, GST, and GR were investigated in all groups. After PTL treatment, MDA, 8-OHdG, and iNOS levels decreased while GSH levels increased in testicular tissue. Increased levels of antioxidant genes and enzymes also reduced oxidative stress. Additionally, the expression levels of the Dazl, Ddx4, and Amh genes, which are involved in gametogenesis and sperm production, decreased in case of toxicity and increased with PTL treatment. The data from this study show that PTL may have a therapeutic effect in the treatment of testicular damage by eliminating the oxidative stress-induced damage caused by PTX in testicular tissue, providing an effective approach to alleviating testicular toxicity, and playing an important role in reproduction/sperm production, especially at a dose of 4 mg/kg.

Złocień maruna – starożytny lek na współczesne choroby

The Feverfew – an ancient remedy for modern diseases The feverfew (Tanacetum parthenium L.), also known as the Chrysanthemum parthenium, is a perennial plant belonging to the Asteraceae family, growing mainly in gardens and open spaces, can be found growing in the regions of Southern Europe and Asia Minor. The healing effects of feverfew were known in antiquity. In folk medicine, this plant has been used to treat fever, migraine headaches, as well as rheumatoid arthritis, stomach and toothache, and insect bites. The active compounds contained in the feverfew include sesquiterpene lactones, and the most important of them – is parthenolide, which is considered to be the main ingredient determining the biological activity of extracts from this perennial. Feverfew preparations, containing parthenolide, reduce the production of prostaglandins, inhibit the release of serotonin and histamine, and antagonize the 5-HT2A receptors, thus showing the anti-migraine effects. Moreover, feverfew extract has anti-infl ammatory properties and reduces allergy symptoms by inhibiting the secretion of histamine from mast cells. Currently, studies are being conducted on the use of parthenolide in the treatment of neuropathic pain. This type of chronic pain is caused by damage to the somatosensory nervous system, which is observed in the development of diabetes, stroke, cancer, or after mechanical injuries (e.g. as postoperative complications or after traffic accidents). In in vivo studies parthenolide showed a strong analgesic effect, moreover, its use allowed for the reduction of morphine doses in neuropathic animals. This important clinical implication raises great hopes for designing a multimodal therapy for neuropathic pain. In addition to its anti-infl ammatory and analgesic properties, parthenolide exhibits antitumor activity by inhibiting the NF-κB and STAT transcription factors and inducing the permanent activation of JNK kinase and p53 protein, thereby sensitizing neoplastic cells to radio- and chemotherapy. Interestingly, parthenolide induces apoptosis of cancer cells but has no toxic effects on normal cells. Currently, preparations from the feverfew are used in the phytotherapy of migraine headaches, however, the results of the preclinical research open up new perspectives for the use of this perennial, in particular parthenolide, in modern therapy.

Phenolic Composition and Antioxidant Activity of Tanacetum parthenium Cultivated in Different Regions of Ukraine: Insights into the Flavonoids and Hydroxycinnamic Acids Profile.

Tanacetum parthenium, also known as feverfew, is rich in bioactive compounds, namely sesquiterpene lactones, flavonoids, and volatile oils. Sesquiterpene lactones possess anti-migraine activity, while phenolic compounds possess anti-inflammatory and antioxidant action. Phytochemical composition determines the pharmacological activity and so profiling is essential in quality assessment. The study aimed to evaluate cultivated feverfew plants' phenolic profiles and antioxidant activity. Eleven phenolic compounds were identified in the samples of feverfew in Ukraine. Hydroxycinnamic acids predominate in the quantitative content of all the samples, namely chlorogenic acid, 3,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid. The total content of flavonoids ranged from 0.8 to 2.6%; the content of hydroxycinnamic acids varied from 3.3 to 6.5%. The obtained data testify to the prospects of using Ukrainian feverfew as a raw material with a significant content of phenolic substances to develop new herbal medicines.

Chromosome numbers in some alien plant species of the Novosibirsk Region: post IV

This paper presents the chromosome numbers (2n) for 12 alien species collected in the Novosibirsk Region from the families Apocynaceae, Asteraceae, Campanulaceae, Caryophyllaceae, Fabaceae, Lamiaceae, Plantaginaceae, Poaceae. For Asclepias syriaca L. (2n = 22), Veronica filiformis Sm. (2n = 14), Setaria italica (L.) P. Beauv. (2n = 18), and Sorghum drummondii (Nees ex Steud.) Millsp. et Chase (2n = 20), chromosome numbers were determined for the first time on the material from Russia; for Tanacetum parthenium (L.) Sch. Bip. (2n = 18), Campanula persicifoliaL. (2n = 16), and Genista tinctoria L. (2n = 48) – from Asian Russia; for Calendula officinalis L. (2n = 32) and Rudbeckia hirta L. (2n = 38) – from Siberia. Chromosome numbers on the material from the Novosibirsk Region were revealed for the first time for Lapsana communis L. (2n = 14) and Silene noctiflora L. (2n = 24). For all the species studied, brief notes on their general distribution and dispersal in the Novosibirsk Region are provided, along with literature data on chromosome numbers from other regions of Russia.

A Novel Approaches to Feverfew (<i>Tanacetum Prthenium</i>): A Review

Tanacetum Parthenium is a herbal medicinal plant which is known as feverfew. Asterace is the family of feverfew. It is traditionally applicable in the treatment of stomach aches, fever, arthritis, infertility, migraine, insect bites, menstruation problems, and labour pain during childbirth. Dioscorides was the first Greek physician to which uses feverfew extract for treatment of inflammation. Particularly among Greek and early European herbalists, feverfew plant has ancient historyfor traditionally used as common medicine. This plant is also known as "featherfew" due to its feathery leaves. The leaves have been used in medicinal preparation in past two decades for treatment of arthritis and migraine by both the British and Canadian government. The herbal plant of feverfew cultivated in large area of world that show medicinal importance. Plant grow substantially and show important strongly report in support of therapeutic uses. This aromatic plant look like yellow green leave and give bitter odour and give therapeutic active chemical constituents include pinenes flavonoid and glycoside. It shows various pharmacological properties, like antispasmodic, cardiotonic, anti-inflammatory, anti-cancer, and as an enema for worms. The colour of feverfew plant is yellow and bloom in October to July, which is also used in asthma, dizziness, nausea and vomiting. Parthenolide may experience an acid-induced cyclisation in the presence of excessive moisture, resulting in a sesquiterpene lactone of the guaianolide class, which is frequently present in feverfew. If the composition satisfies pharmacopoeial microbiological quality standards, microbial degradations are unlikely to have a significant impact.

Formulation and Evaluation of Herbal Facewash : A Step Towards Nature and a Boon to Skin

Acne and pimples are treated using herbal face washes. Herbal Facewash eliminates excess oil without eliminating nutrients from the skin and contains rich plant-based components like feverfew (Tanacetum parthenium) and neem (Azadirachta indica). The building blocks of finished herbal medicines are known as herbal preparations. These can be comminuted or powdered herbal ingredients as well as extracts, tinctures, and fatty oils of herbal materials. The demand for herbal formulations is rising on the global market. Establishing the herbal face wash with aqueous neem leaf, tulsi leaf, and orange peel extracts is a very commendable effort. As a result of checking and evaluating a number of factors, including colour, pH, consistency, washability, irritation, and spreadability, it was determined from the current experiment that the herbal face wash formulation was more effective than the commercial face wash. Since all of the ingredients in this composition are herbal, they are safe for the skin and trustworthy.

Physical and Chemical Properties of Obtained Tanacetum parthenium Extract by Soxhlet System

In this study, freshly collected Tanacetum parthenium in Elazığ (Türkiye) is separated from its stems and cleaned. This sample of prepared is dried in an oven at 60 °C for 4 hours and ground. Extraction has been performed with ethyl alcohol (C2H5OH) for 5 hours using Soxhlet system. In this system, the solvent is heated, evaporated, and the hot vapor of the solvent condenses and falls onto the powder sample. The amounts of sample, working time, and solvent used in the experimental studies have been optimized. Response surface methodology (RSM) is used in optimization studies. The findings of RSM model and experimental data have been statistically assessed. The assessment of the quality of the extract can be evaluated based on its quantity, color, and components. The density and viscosity of the obtained extract have been determined. Besides, as the grain size of Tanacetum parthenium powder decreases, the surface area increases, therefore the extraction time is reduced. The results show that at 78 °C and 5.7 hours, 9.6 grams of biomass has the maximum efficiency. Also, the composition of Tanacetum parthenium extract has been analyzed by gas chromatography−mass spectrometry (GC-MS).

A Study of the Chemical Composition, Acute and Subacute Toxicity of Bulgarian Tanacetum parthenium Essential Oil.

Tanacetum parthenium (L.) Sch.Bip. (T. parthenium) is an aromatic perennial plant belonging to the Asteraceae family, also known as feverfew. It is widely distributed in various regions of Europe and other parts of the world. The plant has a rich background in the traditional medicine of many nations and has been used as a remedy for fever, pain, inflammation, asthma, rheumatism, menstrual disorders, etc. Methods: GC-MS analysis was conducted to determine the chemical composition of the isolated essential oil (EO). Using the method proposed by Litchfield and Wilcoxon, the average lethal dose (LD50) of the EO on Wistar rats was determined for two routes of administration: oral (p.o.) and intraperitoneal (i.p.). The subacute toxicity of the EO was also tested by oral administration of a daily dose of 1.0 g/kg body weight (BW) for 28 days. The toxicity of the EO was evaluated by observing and evaluating changes in behavior, body weight, basic hematological and serum biochemical parameters, and histopathological changes of the internal organs. Thirty-seven volatile organic compounds representing 94.58% of the total oil composition were tentatively detected in the obtained T. parthenium EO. The dominant compounds were camphor (45.47%), trans-chrisantenyl acetate (21.65%), camphene (9.48%), and cis-isogeraniol (5.42%). The results showed that the EO was not toxic when administered in acute oral doses. The acute mean lethal dose for intraperitoneal administration was LD50 i.p. = 2.13 g/kg BW. In the subacute study involving administration of an oral dose of EO for 28 days, there were a number of changes in the hematological and serum biochemical parameters of the blood compared with the control group of animals. However, no symptoms of toxicity, changes in the body weight of the rats, death, or pathological changes in the histological indicators of the examined organs-brain, heart, stomach, liver, spleen and kidney-were found. Extrapolating the results obtained from the rat experiments, we can state that the EO is safe for use in doses below 1 g/kgBW for a period not exceeding one month.

Parthenolide as a potential analgesic in the treatment of paclitaxel-induced neuropathic pain: the rat modeling.

In this study, we determined the therapeutic effect of parthenolide (PTL), the active component of Tanacetum parthenium, on neuropathic pain caused by paclitaxel (PTX), a chemotherapeutic drug frequently used in cancer treatment, at the gene and protein levels. To this end, 6 groups were formed: control, PTX, sham, 1mg/PTL, 2mg/kg PTL, and 4mg/kg PTL. Pain formation was tested by Randall-Selitto analgesiometry and locomotor activity behavioral analysis. Then, PTL treatment was performed for 14days. After the last dose of PTL was taken, Hcn2, Trpa1, Scn9a, and Kcns1 gene expressions were measured in rat brain (cerebral cortex/CTX) tissues. In addition, changes in the levels of SCN9A and KCNS1 proteins were determined by immunohistochemical analysis. Histopathological hematoxylin-eosin staining was also performed to investigate the effect of PTL in treating tissue damage on neuropathic pain caused by PTX treatment. When the obtained data were analyzed, pain threshold and locomotor activity decreased in PTX and sham groups and increased with PTL treatment. In addition, it was observed that the expression of the Hcn2, Trpa1, and Scn9a genes decreased while the Kcns1 gene expression increased. When protein levels were examined, it was determined that SCN9A protein expression decreased and the KCNS1 protein level increased. It was determined that PTL treatment also improved PTX-induced tissue damage. The results of this study demonstrate that non-opioid PTL is an effective therapeutic agent in the treatment of chemotherapy-induced neuropathic pain, especially when used at a dose of 4mg/kg acting on sodium and potassium channels.

Parthenolide targets NLRP3 to treat inflammasome-related diseases

Natural products have attracted extensive attention from researchers in medical fields due to their abundant biological activities. Parthenolide (PTL) is a sesquiterpene lactone originally purified from herb Feverfew (Tanacetum parthenium), recent studies have showed its potential activities of anti-cancer and anti-inflammatory. Acting as the most studied inflammasome, NLRP3 inflammasome played an important role in human diseases including type-2 diabetes (T2D), Alzheimer's disease (AD) and cryopyrin-associated periodic syndromes (CAPS). In this article, we show that PTL specially inhibits the activation of NLRP3 inflammation by block the upstream signal and prevent the assembly of NLRP3 inflammasome complex. Furthermore, we showed the treatment of PTL significantly attenuates the symptoms of lipopolysaccharide (LPS)-induced systemic inflammation and dextran sulfate sodium (DSS)-induced colitis in mice models. Thus, our results demonstrate that PTL alleviates inflammation by targeting NLRP3 inflammasome, which indicate that PTL acting as a promising natural product for the treatment of NLRP3 inflammasome-related diseases.

In vitro analysis of green synthesized CuO nanoparticles using Tanacetum parthenium extract for multifunctional applications.

Tanacetum parthenium L. is a popular traditional medicinal plant that the role of presence of particular phytochemical compounds are still unconsidered particularly in the bio-nano researches. Here, for the first time, the green fabrication of CuO NPs using Tanacetum parthenium L. extract was performed and assessed for the antimicrobial, cytotoxicity, and dye degradation activities. Characterization of CuO NPs was done by UV-visible spectra, XRD, FT-IR, TEM, and EDX. The synthesized CuO NPs possess a crystalline nature, a functional group that resembles T. parthenium, with a spherical shape particle with an average size of 28nm. EDX confirmed CuO NPs formation. The CuO NPs showed excellent antimicrobial activity against tested microorganisms. The cytotoxicity of CuO NPs was demonstrated the concentration-dependent inhibition of the growth against both cancer and normal cell lines. The results exhibited concentration-dependent inhibition of the growth of Hela, A 549, and MCF7 cancer cells (IC50 = 65.0, 57.4, and 71.8µg/mL, respectively), which were statistically significant comparing control cells (IC50 = 226.1µg/mL). Furthermore, we observed that CuO NPs-induced programmed cell death in the cancer cells were mediated with the downregulation of Bcl2 and upregulation of bax, caspase-3. CuO NPs were verified to be a superb catalyst as they had excellent activity for the degradation of 99.6%, 98.7%, 96.6%, and 96.6% of Congo red, methylene blue, methylene orange, and rhodamine B as industrial dyes in 3, 6.5, 6.5, and 6.5min, respectively. Overall, the present study nominates T. parthenium as a proper bio-agent in the biosynthesis of CuO NPs with powerful catalytic and antimicrobial activities as well as a cancer treatment.

Development and validation of methods of analysis of active substances in emulgel with thick extract of tanacetum parthenium

Development and validation of methods of analysis of active substances in emulgel with thick extract of tanacetum parthenium

The substantiation of the composition of a soft medicine for the therapy of inflammatory diseases of the musculoskeletal system

Aim. To substantiate the optimal composition of the base of the semisolid dosage form with the thick extract of feverfew (Tanacetum parthebased on structural-mechanical, textural, adhesive and microscopic studies. Materials and methods. The study objects were samples of a semisolid dosage form with the thick extract of feverfew with various gelling agents and the reference drug “Irikar”. In the course of the research, a complex of structural-mechanical methods and the microscopic analysis of the samples were used. Results and discussion. The studies of structural-mechanical properties have determined the regularities in the rheological characteristics of the experimental samples made using different gelling agents. It has been found that the rheogram of the emulgel sample has a smooth transition from the elastic deformation region to the elastic-plastic one, which may indicate a more homogeneous distribution of the sample components. It has been also determined that the sample corresponds in the textural properties to the refenium) rence drug – “Irikar” cream. The structural-mechanical studies have substantiated the rational content of the gelling agent in the emulgel composition – 3 %. The results of rheological studies have been confirmed by the data of the microscopic studies. Conclusions. The data obtained are the basis for further pharmaceutical development of a drug with the thick extract of Tanacetum parthenium for the treatment of diseases of the musculoskeletal system.

Phytochemical composition, toxicity, and repellent effects of medicinal plants Peganum harmala, Pterocarya fraxinifolia, and Tanacetum parthenium extracts against Sitophilus oryzae L.

Sitophilus oryzae is an important pest of stored rice and is usually controlled by the use of aluminum phosphide, which is dangerous to humans. Botanical insecticides deemed promising alternatives in controlling this pest. In this study, extracts of Peganum harmala seeds, Pterocarya fraxinifolia leaves, and Tanacetum parthenium flowers were evaluated against S. oryzae adults. Phytochemical analyses showed that steroid, terpenoid, tannin, alkaloid, and flavonoid are present in the extracts. Bioassays revealed repellency effects of all extracts, besides, P. harmala extract exerted significant mortality (93.33 ± 6.6%, LC50: 521.06 mg/ml) hence it may have applications in preventing infestation of stored rice by S. oryzae. Highlights Plant extracts studied in this research efficiently repel rice weevil. These plant extracts show significant toxicity against rice weevil. Alkaloid, steroid, terpenoid, tannin, and flavonoid are present in extracts of these plants.

New combination and typification of Tanacetum parthenium var. flosculosum (asteraceae) and its first record from turkey

In this study, a discoid capitulate variety of Tanacetum parthenium was recorded for the first time in Turkey. Additionally, the new combination of the name is given as Tanacetum parthenium var. flosculosum. Moreover, the varietal name was neotypified with material from Vaillants? collection at P.

Phytochemical and physiological reactions of feverfew (Tanacetum parthenium (L.) Schultz Bip) to TiO2 nanoparticles

Nanomaterials can be used as elicitors for improving the biosynthesis of secondary metabolites in medicinal plants. The present study was conducted to assay the titanium dioxide-nanoparticles (TiO2-NPs) effects on feverfew (Tanacetum parthenium) as an anti-cancer plant. The study showed that TiO2-NPs application increased the amounts of the main compounds and oxygenated monoterpene in essential oils, thereby causing an improvement in the quantity and quality of the essential oils compared to control. The highest effect was related to 1500 ppm TiO2-NPs concentration. Regarding parthenolide, TiO2-NPs had no positive effect on parthenolide content and the highest content was observed in control. Increasing the concentrations over 1500 ppm resulted in a decrease in chlorophyll content, capitule diameter, flower yield, and harvest index compared to other concentrations and control. Additionally, the results indicated that TiO2-NPs foliar spray reduced flower number, biological yield, fresh weight, and dry weights compared with untreated plants. The increase in quality and content of essential oil and lack of increase in parthenolide content, and reproductive and vegetative characteristics showed that TiO2-NPs mainly affected the content and composition of essential oil. Totally, the application of TiO2-NPs in terms of positive effect on the yield and metabolites (without damaging biological effects) can be recommended and followed up to the concentration of 1000 ppm. Overall, the results indicated that improving the synthesis of valuable medicinal metabolites using TiO2-NPs has promising results depending on the type of species, concentration used and target metabolites.

Evaluation of anticoagulant and inflammatory effects of Tanacetum parthenium (L.) in a randomized controlled clinical trial

IntroductionThe use of aspirin in the primary prevention of diseases is debatable. Herbal products might be considered potential substitutes for synthetic chemicals in many situations. Tanacetum parthenium or feverfew is widely used to treat various inflammatory diseases, fever, and headaches. But could it be considered a herbal alternative to aspirin? This double-blind randomized clinical trial was designed to evaluate the effects of feverfew on various immunological and coagulation factors in healthy individuals and to assess its potential adverse effects. MethodsAmong healthy and non-smoking adult men of Fasa (IRAN), 60 individuals were randomly recruited into control and intervention groups. Participants consumed one capsule (250 mg) of Tanacetum parthenium or placebo every day for 2 weeks. Blood samples were taken from everyone and inflammatory cytokines, prothrombin time (PT), partial thromboplastin time (PTT), and liver function tests were assessed. T-test, Chi-square (X2) distribution, and linear regression analyses were done. ResultsThe increased serum level of PT was shown in the intervention group (12.2 ± 0.7 s) compared to the control group (11.6 ± 0.6 s) in the t-test (p = 0.003) and the regression model (p = 0.04). PTT (41.8 ± 5.7 s compared to 33.0 ± 5.1 s), IL-17A (28.0 ± 28.6 compared to 18.9 ± 15.9 pg/ml) and Chemokine MCP-1 (1061.3 ± 705.3 compared to 714.2 ± 273.0 pg/ml) also showed a significant increase in the intervention group after consumption of feverfew (P values= 0.001, 0.048 and 0.017 respectively). ConclusionThe data suggested that Tanacetum parthenium (L.) may have anticoagulant properties. It is recommended that more studies with greater sample sizes and longer follow-ups be done in this regard.

Recent advances on the structural modification of parthenolide and its derivatives as anticancer agents

Parthenolide (PTL) is a sesquiterpene lactone derived from medicinal plant feverfew (Tanacetum parthenium). Recent studies have demonstrated that it has multiple pharmacological activities, especially in the treatment of various hematological and solid cancers. The superior anticancer activity of PTL suggests that it has the potential to be a first-line drug. However, due to the limited physical and chemical properties, as well as bioavailability, structural modification strategies are strongly recommended to improve the anticancer activity. This review describes representative PTL derivatives obtained by different modification strategies, which are reported to exert antiproliferative activities superior to the parent compound PTL. Furthermore, we also summarize their basic mechanisms on cancer-related signaling pathways, so as to explain the potential and characteristics of PTL and its derivatives in cancer therapy.

Research on the phenolic profile, antiradical and anti-inflammatory activity of a thick hydroalcoholic feverfew (Tanacetum parthenium L.) herb extract

The aim – to study the phenolic complex of a thick hydroalcoholic extract of the feverfew (Tanacetum parthenium (L.) herb (FTHAE), its antiradical activity and anti-inflammatory properties in a model of carrageenan and histamine oedema.
 Materials and methods. The studied extract was obtained from the Tanacetum parthenium herb, collected in Sumy and Poltava regions of Ukraine during the period of mass flowering (June-August): degree of grinding of raw materials 2.0-3.0 mm, extraction temperature - 25 °C, extractant – 70 % ethanol, raw material/extractant ratio – 1:12, infusion time – 12 hours, multiplicity of extractions – 3. HPLC and spectrophotometric methods were used to determine the composition and amount of phenolic compounds of FTHAE. HPLC analysis was performed using a “Waters e2695 Alliance system” (Waters, Milford, MA, USA) with a photodiode array detector “Waters 2998” according to the HPLC–PDA method for phenolic compounds. The scavenging of ABTSA radical cation evaluated the radical scavenging activity. In addition, the anti-inflammatory properties of FTHAE were studied on carrageenan and histamine paw oedema in rats. Anti-inflammatory activity (AIA) was evaluated as the ability to reduce oedema compared to the control pathology. FTHAE was used at a dose of 50 mg/kg.
 The results. The content of the sum of hydroxycinnamic acids in the obtained extract was determined by spectrophotometry, which was 13.92±0.02 % and the content of the sum of flavonoids – 5.16±0.03 %. The content of 12 compounds with a total amount of 72432.09µg/g was identified and determined by HPLC. The dominant compounds were hydroxycinnamic acids, namely 3,4-dicaffeoylquinic, 4,5-dicaffeoylquinic and сhlorogenic acids. The antiradical activity of the extract was 620.19±4.53µmol/g. On the model of carrageenan oedema, the maximum effect of oedema suppression was 71.0-73.2 %. In the model of histamine oedema, the anti-inflammatory effect of the extract was 57.8; 51.8; and 49.1 % for 30 minutes, 1 and 1.5 hours of oedema, respectively. The severity of the anti-inflammatory activity of the extract during the first hour is not inferior to the diclofenac sodium, quercetin and loratadine.
 Conclusions. Due to the HPLC method, 12 compounds were determined to cause antiradical activity, among which chlorogenic acid and rutin were identified.
 The studied extract has a pronounced anti-inflammatory effect, which is due to the antiradical properties of the extract and its inhibitory effect on inflammatory mediators