The remote ischemic preconditioning (R-IPC) has shown protective effect by reducing the activation of lymphocytes and the production of inflammatory cytokines in a model of ischemia and reperfusion of lower limb. In experimental intestinal transplantation, N-acetylcysteine (NAC) reduced plasma levels of TNF-α and IL-8. The aim was to evaluate the role of the remote ischemic preconditioning or N-acetylcysteine on inflammatory response after fetal intestinal transplantation. Methods: It was used pregnant mice from BALB/c strain to obtain fetal intestine graft to transplant in BALB/c (ISO) and C57BL/6 (ALO) adult mice, without immunosuppression. Pregnant were submitted to remote ischemic preconditioning 10x10 or to N-acetylcysteine (150mg/kg) administration. At the 7th post-operative day, the intestinal graft was harvested to seek the cytokines expression. The mRNA expressions of IFN-γ, IL-10 and IL-6 of the lymph nodes were evaluated by real-time PCR. Expression levels were standardized for GAPDH (housekeeping) gene expression. Results: We observed that endogenous IFN-γ mRNA expression were significantly lowered by both strategies on iso and allogeneic transplant. The endogenous IL-10 mRNA expression and the treatment increased IL-10 expression on ISO/R-IPC (˜3,0 folds) and ISO/NAC (˜2,3 folds) compared with control group (ISO). We also noted that R-PCI induced a high level of interleukin-6 mRNA only on ISO group when compared to others groups (˜3,5 folds). Conclusion: Remote as well as N-acetylcysteine reduced inflammatory response in fetal intestinal graft.