Fertility Potential Research Articles

Dynamics of the content of mineral elements and effective fertility of alluvial meadow soil in vegetable crop rotation

In vegetable crop rotation with different fertilization systems of vegetable crops, the dynamics of the content of mineral elements of plant nutrition was determined, effective soil fertility was evaluated in comparison with potential under experimental conditions, and the most effective fertilizer system was identified not only to obtain the highest yields, but also to preserve and reproduce the potential fertility of alluvial meadow soil. The study was conducted in 2015–2019 in the Ramensky district of the Moscow region on the alluvial meadow medium loamy soil of the central floodplain of the Moscow River. The seasonal dynamics of the content of 3 elements of plant mineral nutrition (nitrate nitrogen, mobile phosphorus and potassium) in the carrot – beet – cabbage crop rotation was studied, using 3 main fertilization systems for vegetable crops (mineral, organic, organo-mineral). It was found that the maximum content of nitrate nitrogen (on average in crop rotation 47.4 mg/kg) and mobile potassium (on average 156 mg/kg) occurred in the third decade of June. The content of mobile phosphorus in the soil was high (200 mg/kg) at all accounting periods. A close correlation was determined between the yield of root crops and cabbage with the average content of nitrate nitrogen in the soil during the growing season (r = 0.735–0.934), mobile phosphorus (r = 0.539–0.972) and potassium (r = 0.532–0.976). The effective soil fertility of the site under 3 crops per crop rotation had the following characteristics: when using a mineral fertilizer system, the content of nitrate nitrogen was 21.3, organic – 10.1, organo-mineral – 22.1 mg/kg, the content of mobile phosphorus when using a mineral system – 40.4, organic – 34.1, organo-mineral – 47.9 mg/kg, the content of mobile potassium is 44.4, 20.6, 50.8 mg/kg, respectively. It was found that under crops of carrots and beets, the potential fertility of the soil during crop rotation was reproduced with the use of mineral (recommended dose of NPK) and organo-mineral fertilization systems of these crops, and the yield of carrots (67–69 t/ha) and beets (67–68 t/ ha) under these systems was high, more than expected estimated 60 t/ha. Under cabbage, late soil depletion also did not occur when using the recommended mineral and organo-mineral fertilizer systems, yields of 78 and 81 t/ha were close to the expected estimated yield of 80 t/ha. At the same time, the organo-mineral system had an advantage: during crop rotation, it retained 13% more nitrate nitrogen in the soil, 8% more mobile phosphorus and 40% more mobile potassium than the mineral one. One and a half and double doses of mineral fertilizers for late cabbage led to a significant increase in its yield (85–90 t/ha), but at the same time a significant part of nitrogen (on average for the season 33–41 mg/kg) was irretrievably lost in the soil.

Thermodynamic and energy characteristics of carbonate soil-forming rocks and soils of the Western Ukrainian Region

The current stage of development of soil science is characterized by the intensive development of the energy-thermodynamic approach and the solution of theoretical and methodological issues that are directly related to the problem of assessing the energetics of soil formation. The article analyzes the influence of components of the geographical environment as factors of soil formation potential, highlights the features of the formation of various types of energy costs and energy balance during soil formation, and presents the energy and thermodynamic characteristics of carbonate soil-forming rocks and soils of the Western region of Ukraine. Based on energy and thermodynamic indicators (crystal lattice energy, Gibbs free energy and entropy), the ability to intensive soil formation and the suitability for biological development is determined by the characteristics of the studied soil-forming rocks, such as: eluvium of Turonian-Senonian deposits of writing chalk, eluvium of Turonian-Senonian deposits of chalk marls, eluvium of lithothamnian limestones of the Upper Badenian, eluvium of chemogenic limestones of the Upper Badenian, eluvium of clotted limestones of the Upper Badenian. The highest values of crystal lattice energy are characterized by short-profile and full-profile Rendzinas on eluvium of clotted limestones of the Upper Badenian within the Roztochchya-Opilya Upland Region. Significant reserves of internal energy in these soils are due primarily to the highest values of the energy of the crystal lattice of the soil-forming rocks on which they were formed. The lowest values of thermodynamic characteristics are observed in poorly developed and short-profile Rendzinas (Rendzic Leptosols, WRB) within the Voronyaky natural district of the Western Podilsky upland region, formed on the eluvium of Turonian-Senonian deposits of Cretaceous marl. Therefore, significant variability of internal energy in rendzins is due to the influence of various carbonate soil-forming rocks. The proposed results of the study of soil-forming energetics are extremely relevant, as they contribute to a scientifically substantiated solution to one of the most important problems of modern soil science, optimization of the level of soil safety management and reproduction of their potential fertility. Key words: energy-thermodynamic approach; soil formation potential; carbonate soil-forming rocks and soils; potential fertility.

Potential of Biochar-Based Fertilizers for Increasing the Productivity of Okra in Gajuri, Dhading

Sustainable agricultural production depends on increasing crop productivity while preserving soil health and reducing environmental risk. The purpose of this study was to evaluate the potential of biochar (10 t ha-1) based organic and inorganic fertilizer for increasing okra productivity through a field experiment conducted in Gajuri, Dhading. A 130 m2 area was divided into six treatment groups, each with four replications, using a Randomized Complete Block Design. The following were the treatments: i) inorganic fertilizer (RF); ii) biochar plus inorganic fertilizer (BF); iii) biochar plus vermicompost (BVC); iv) biochar plus poultry manure (BPM); v) biochar (BC); vi) control; neither biochar nor fertilizer (CK). The recommended rates of urea, di-ammonium phosphate (DAP), and muriate of potash (MOP) were applied to the mineral NPK fertilizers in RF and BF. The rate whereby organic fertilizers were applied was 200 kg N ha-1. Plots treated with biochar and various fertilizer groups were compared in terms of growth and yield efficiency. The BVC treatment was found to exhibit poorer growth performance in terms of plant height, number of leaves, primary branches, and nodes compared to the combination of biochar and poultry manure. Fruit output rose by 170% over CK (7.13 mt ha-1) and by 53.26% over RF (12.58 mt ha-1) after BPM treatment (19.28 mt ha-1). While BF and RF did not significantly differ in terms of growth characteristics, BF outproduced RF by 29% and CK by 126.79% in terms of pod yield. BF and BPM offered greater financial rewards than alternative treatments.

Exploring Potential of Organo-mineral Fertilizers in Augmenting Crop Yield and Quality – A Review

Inorganic fertilizers are essential for improving crop yield and quality through enhanced crop efficiency. However, their continuous use often causes nutrient imbalances, leading to poor yield and quality. Organic manures, while effective in improving soil fertility and crop productivity, face challenges such as limited availability, variable quality, transportation issues, heavy metal contamination and slow nutrient release. The exclusive dependence of either organic or inorganic fertilizers has not adequately fulfilled the increasing demands for enhanced agricultural output and quality. To address these challenges, organo-mineral fertilizers (OMFs) have emerged as an innovative solution. OMFs are derived from the combination of mineral and organic nutrient sources, integrating the benefits of both. This low input technology supplies nutrients in balanced quantities that are readily and steadily available throughout the growing season. OMFs improve soil physical properties, enhance fertility and support sustainable crop production. They also promote plant resistance to biotic and abiotic stress, offering a holistic approach to agricultural challenges. The development of OMFs aligns with the recycling of urban, industrial and agricultural residues, reducing dependency on raw materials for chemical fertilizers and contributing to environmental sustainability. Advances in OMF preparation, using various mineral and organic matrices, have demonstrated their ability to address nutrient deficiencies, particularly in tropical soils, where poor fertility often limits productivity. OMFs enhance soil physico-chemical and biological properties, addressing soil degradation while improving crop yield and quality. They ensure an optimal supply of nutrients, support long-term soil health and align with modern fertilizer management strategies aimed at sustainable agricultural productivity. In conclusion, OMFs are a promising solution for boosting crop quality and yield while maintaining soil fertility. By incorporating the advantages of organic and inorganic inputs, they provide a balanced, sustainable, and efficient strategy to address growing demands for food production.

YIELD AND PHYSIOLOGIC POTENTIAL OF PODS BEAN SEEDS ACCORDING TO ORGANIC COMPOST DOSES

The production of high-quality pods bean seeds in the organic production system is a challenge for farmers because there is little information on the appropriate doses of organic compounds to be recommended in the literature. This study aimed to evaluate the yield and physiologic potential of pods beans according to the application of doses of organic compost (earthworm humus). The experimental design used was completely randomized, and the treatments were different doses of organic compost (0, 30, 60, 90, and 120 t ha-1), with four replications. Different yield components were evaluated, and the seed quality was analyzed by the main vigor and germination tests. Organic compost doses influenced most of the seed yield components; however, the physiologic potential and organic fertilization influenced only 1000-seed weight and seedlings dry matter. It is concluded that the doses of organic compost promote the increase in pods bean yield but do not influence the physiologic potential of seeds.

Assessment of Land Suitability for Enhancing Key Crop Commodities: Pineapple, Coffee, and Mango

This study aims to evaluate land suitability for enhancing the production of key crop commodities in Ngancar District, Kediri Regency, particularly pineapple, coffee, and mango. The district has high soil fertility potential due to its location at the foot of Mount Kelud. The research methods include land surveys, soil sampling at depths of 0-30 cm and 30-60 cm, and laboratory analysis to measure soil physical and chemical properties. Results indicate that the land in the study area generally falls into the marginal suitability class (S3), with the main limiting factors being soil texture dominated by sandy loam, low K₂O levels, and high rainfall that affects pineapple growth. Slope gradient is also a limiting factor for coffee and mango cultivation. Adjustments in soil management, such as timely fertilization and planting crops with strong root systems, can improve the land's suitability potential. In conclusion, although the land in Ngancar District has certain limitations, optimizing land use can enhance the productivity of key commodities, particularly through improved soil management and appropriate fertilizer use. Keywords: Crop commodities, Land Assessment, Land Degradation, Mount Kelud.

The lordscape: Mapping seigneurial jurisdictions in the late-medieval Low Countries

This article explores the relationship between the spatial distribution of elite power and geophysical factors in two regions within the Low Countries between c.1350 – c.1650. It does so through a focus on seigneuries, bundles of territory and rights through which premodern lords and ladies across Europe held jurisdiction and economic prerogatives over local subjects. Historians have often assumed that the uneven distribution of such jurisdictions in different regions was connected to the fertility and commercial potential of the landscape. This article pioneers a structured, transregional approach to test this hypothesis, by quantifying and visualizing the spatial distribution of seigneuries in connection with three geophysical features – soil fertility, proximity to waterways, and relief – within and between two Netherlandish principalities, Guelders and Hainault. Through visualization and quantification of the spatial clustering of seigneuries, the analysis confirms that these institutions gravitated towards areas that were most fertile and commercially viable. In addition, the data suggest a hierarchy in the relative importance of geophysical features. Soil fertility emerges as the prime factor, with relief (Hainault) and waterways (Guelders) as secondary (interdependent) factors.

Alleviation of Fe-induced chlorosis of soybean plants grown in calcareous soil by a freeze-dried iron fertilizer containing siderophores produced by Rhizobium radiobacter

The concerns about the unsustainability of traditional synthetic Fe chelates lead to the search for new environmentally friendly alternatives, such as siderophores-based fertilizers. In this work, the focus was on the evaluation of a bio-based Fe-chelate fertilizer obtained from the culture of the siderophore-producer bacterium Rhizobium radiobacter. The suitability of a freeze-dried fertilizer formulated from a R. radiobacter culture labeled with 57Fe to alleviate Fe chlorosis in soybean plants cultivated in calcareous soil was analyzed and the new potential Fe fertilizer was evaluated in comparison to the traditional synthetic chelate o,oEDDHA/57Fe3+. This natural chelate was able to maintain chlorophyll content stable during all the pot trials and presented greater Fe concentration in the remaining soil fractions serving as an Fe pool for a long time whereas o,oEDDHA/57Fe3+ could supply Fe quickly. The new bio-based Fe siderophore fertilizer, derived from R. radiobacter culture, could be a green substitute to conventional synthetic chelates to address Fe chlorosis in calcareous soil conditions.

Taxifolin: A flavonoid in the freezing medium augments post-thaw semen quality and in vivo fertility potential of buffalo bull spermatozoa

This comprehensive study was carried out to investigate the effects of taxifolin in the freezing medium on post-thaw semen quality, and fertility potential of buffalo bull spermatozoa. Taxifolin was also evaluated for radical scavenging activity through DPPH (2, 2-diphenyl-1-picrylhydrazyl), and Nitric oxide (NO) inhibition (%) during in vitro conditions. Collected semen samples from four buffalo bulls were initially evaluated (consistency, volume, motility, and concentrations); the accepted samples were pooled, and diluted in extenders containing different doses of taxifolin (0 μM [control], 2 μM, 5 μM, 10 μM, and 20 μM). Diluted semen was gradually cooled (2 h, 4 °C), equilibrated (4 h, 4 °C), and then frozen in liquid nitrogen (−196 °C). Data analysis revealed that taxifolin supplementation (10 μM) displayed the highest DPPH-scavenging ability, and NO inhibition compared to the control (% DPPH, 67.31 vs. 13.50; and % NO, 78.25 vs. 21.25) respectively. Moreover, taxifolin supplementation (10 μM) significantly (P < 0.05) improved progressive motility (%), average path velocity (μm/sec), straight-line velocity (μm/sec), and seminal plasma glutathione peroxidase (μM), and superoxide dismutase (U/mL) of buffalo bull spermatozoa than control. Sperm plasma membrane integrity, mitochondrial transmembrane potential, acrosome integrity (%) and seminal plasma adenosine triphosphate (nmol/million), and total antioxidant capacity (μM/L) were enhanced significantly with taxifolin (10 and 20 μM) supplementing group. Lastly, taxifolin supplementation significantly improved the fertility rate (%, 69.09 vs. 40.38) compared to the control. Further studies to assess mechanisms by which taxifolin improves semen quality and fertility of buffalo spermatozoa are warranted.

Anaerobic decomposition of substandard pet food as a raw material source for producing hydrogen from methane

A potential raw material for producing hydrogen (the main carrier for the accumulation, storage and transportation of energy) is methane from biogas. An approach to producing biogas with a high methane content (69–72%) from waste commercial dry and wet food for dogs and cats under mesophilic conditions has been demonstrated. For 27–28 days under anaerobic conditions, the degree of biotransformation of waste was 60–88%. As a result of mineralization of watered organic waste, the content of ammonium nitrogen and phosphorus in the form of phosphates amounted to 676–887 mgNH4+/l and 77–160 mgPO43-/l, respectively. In anaerobically treated effluent, accumulation of sulfide ions up to 22 mg/l was observed. The solid sediment and anaerobically treated effluent (liquid fraction) obtained upon completion of the biotransformation of pet food waste are a potential organic fertilizer for agricultural needs, and methane from biogas is a raw material for producing hydrogen and pure carbon for the needs of the nanoindustry.

Inorganic and organic additives differently regulate compost microbiomes in response to heavy metals immobilization

Various composting additives, both organic and inorganic, have been used to effectively reduce the bioavailability of heavy metals (HMs) in compost. However, a comprehensive assessment of their comparative contributions and the mechanisms involved is still lacking. In this study, we selected shell powder and biochar as representative amendments to assess their efficacy in immobilizing various HMs. Our results demonstrated that the addition of shell powder and biochar markedly enhanced the synthesis of humic acids (HA), reduced the bioavailability of HMs, and indirectly enhanced microbial carbon metabolism and growth pathways. This enhancement subsequently enriched the abundance of key HA synthesis contributors, including Enterobacter, Thermobispora, Pseudomonas, and Sphingobacterium, creating a positive feedback loop. Additionally, adding biochar altered the abundance of Pseudomonas, Chelatococcus, and Pseudoxanthomonas during the mesophilic phase, thereby reducing the expression of HMs resistance genes (HMRGs). In contrast, shell powder supplementation modulated the response to HMRGs reduction by regulating the abundance of Sphingobacterium, Pseudomonas, and Thermobispora during the thermophilic phase. Ultimately, regional cultivation trials confirmed the potential of these innovative organic fertilizers to significantly reduce the spread of HMs to plants. Collectively, these findings highlighted the similarities and differences between organic and inorganic additives to modulate microbial community responses during HMs immobilization, providing a theoretical framework for diminishing the risks associated with HMs dissemination and improving the quality of organic fertilizers.

Panax ginseng nanoemulsion for counteracting male infertility via modulating sex hormones and oxidative stress in a rat model

This study end to develop nanoemulsions of Panax ginseng dry extract and to evaluate the potential impact of these nanoemulsions versus free Panax ginseng dry extract and Vit.E in recovering male infertility induced in rats. Nanoemulsions of Panax ginseng dry extract were prepared by oil in water method. The designed samples were characterized by TEM, zeta sizer, FTIR, and TGA. The in vitro study included DPPH assay to estimate the free radical scavenging activity of the suggested treatments. The in vivo study included 100 adult male Wistar rats which were assigned into 10 equal groups; five groups of young rats weighting (150–200 g) and five groups of aged rats weighting (350–400 g). Group I, negative control. Group II, bisphenol-A (BPA). Group III, BPA+ Panax ginseng dry extract nanoemulsion. Group IV, BPA+ free Panax ginseng dry extract. Group V, BPA +Vit.E. After 40 days, serum total testosterone, free testosterone, MDA, 8-OHdG and AGEs were estimated. Besides, the histological investigation of testicular tissue sections was performed. TEM imaging of Panax ginseng dry extract nanoemulsions indicated spherical shape with diameter range from 2 to 50 nm, and the size distribution was in the range from 62 to 123 d.nm. The zeta potential of the designed nanoemulsions was -32.8 to -38.9 mV. FTIR spectra revealed the common active groups in the prepared nanoemulsions. The thermal stability of the nanoemulsions was up to 207 ºC. The in vitro results of DPPH assay showed % inhibition of DPPH free radical for Panax ginseng nanoemulsions samples was 49.38% (for young-treated group Sample A) and 72.28% (for aged-treated group Sample B), while for free Panax ginseng dry extract samples was 30.27% (for young-treated group Sample C) and 56.76% (for aged-treated group Sample D), for Vit.E samples was 32.36% (for young-treated group Sample E) and 36.39% (for aged-treated group Sample F).Thus the nanoemulsions exhibit free radicals scavenging activity more than free Panax ginseng dry extract and Vit.E. The in vivo findings elucidated that Panax ginseng dry extract nanoemulsions and Vit.E successfully revers the progressive insult of BPA on male fertility by significantly enhance total testosterone (2.87±0.318) and free testosterone (1.63±0.033) serum levels, and significantly decrease MDA (2.77±0.018), 8-OHdG (6.76±0.174) and AGEs (92.60±1.701) serum levels. Interestingly, the most promising outcomes were recorded upon the treatment with Panax ginseng dry extract nanoemulsions. In conclusion the developed Panax ginseng dry extract nanoemulsion could be used as a promising strategy in improving potential male infertility defects by rescuing male sex hormones, neutralizing oxidative stress and retrieving the structural organization of the testes.

Primary ciliary dyskinesia and male infertility: Unraveling the genetic and clinical nexus.

Primary ciliary dyskinesia (PCD) is a rare autosomal recessive disorder characterized by dysfunction of motile cilia in various organ systems, including the respiratory and reproductive tracts. A key manifestation in males is infertility, primarily attributed to impaired sperm motility. Although sperm vitality may be preserved, immotility or abnormal flagellar function significantly impairs natural conception. While a minority of men with PCD achieve fatherhood without medical intervention, most require assisted reproductive techniques (ART). The genetic etiology of PCD-related male infertility is rooted in mutations affecting dynein arms and other axonemal components essential for ciliary movement. Diagnostic evaluation relies on semen analysis, ultrastructural ciliary assessment, and genetic testing. Sperm vitality testing is critical when motility is severely compromised, providing crucial insight into fertilization potential. Management of male infertility in PCD predominantly involves ART. Intracytoplasmic sperm injection (ICSI) is particularly effective, as it circumvents the requirement for sperm motility. In cases where spermatozoa retrieval from ejaculate is not feasible, testicular sperm extraction (TESE) offers a viable alternative. Advances in diagnostic techniques and personalized therapeutic approaches have significantly improved fertility outcomes for PCD patients. Ongoing research into genetic therapies and targeted treatments holds promise for further enhancing reproductive success in this population. This review offers a comprehensive examination of the current knowledge surrounding PCD and its impact on male fertility, elucidating the genetic mechanisms, diagnostic challenges, and evolving therapeutic strategies in the management of PCD-associated male infertility.

Broiler breeder putative lipid biomarkers associated with sperm mobility.

Biomarkers indicative of sperm mobility in broiler breeders would provide the ability to screen for fertility potential, with a positive correlation established between sperm mobility and fertilization potential. This study characterized the lipidome of seminal plasma (SP), sperm cell (SC), and whole semen (WS) isolated from broiler breeder roosters with different sperm mobility phenotypes across key timepoints of the semen production cycle. WS samples were collected from five high mobility roosters and five low mobility roosters during early, mid, and late semen production, with SP separated from SC by centrifugation. Using multiple reaction monitoring (MRM) profiling, a total of 3241 lipid species were identified in rooster semen across ten lipid classes. Metaboanalyst 6.0 was used to analyze the relative ion intensity for each lipid species due to sperm mobility phenotype through a t-test and due to timepoint through a one-way ANOVA, with lipid ontology enrichment analysis performed using LION. Metaboanalyst 6.0 was also used to perform biomarker analysis for the sperm mobility phenotype in WS samples. Lipid class total abundance differed with sample type, sperm mobility phenotype, and timepoint. A total of 31, 99, and 112 lipid species were found to be different between low and high mobility males across timepoints in the SP, SC, and WS samples, respectively. Lipid ontology enrichment analysis revealed stark contrasts in lipid-based functions key to sperm survival, storage, and productivity between low and high sperm mobility phenotypes. Through biomarker analysis, 8 lipid species were identified as excellent sperm mobility biomarkers that could be detected in early and mid-semen production. Timepoint based changes in lipid species were unique to each sperm mobility phenotype, with low sperm mobility roosters exhibiting a larger number of lipid species changes over the semen production cycle in the SP and SC when compared to high sperm mobility roosters. This is the first study to characterize poultry semen lipidome using MRM profiling. The lipid species identified between low and high sperm mobility roosters could be utilized in the poultry industry as potential biomarkers of fertility potential, with the ability to screen for the economical trait of fertility potential early in semen production.

Microbial diversity and biosafety judgment of digestates derived from different biogas plants for agricultural applications

The composition of microbial communities is the key to effective anaerobic digestion (AD). The microbiome driving the AD process has been extensively researched, whereas the influence of specific substrates on the microbiome of digestate remains insufficiently investigated. Digestate has considerable potential for use in soil fertilization and bioremediation, therefore its biological safety should be monitored. Moreover, the knowledge about the composition of microbial communities and their interconnections in digestate should be extended, due to the impact on soil microbiota and its functionality. The aim of this study was a comprehensive assessment of the (1) sanitary quality, (2) core microbiome, and (3) microbial interactions in digestates collected from three full-scale agricultural biogas plants, with particular emphasis on their applicability from the perspective of the resident microbiota. Analyzed samples of digestate were derived from various substrates used for AD, including plant- and animal-based materials, and industrial waste. The study demonstrated that the phyla Bacillota, Bacteroidota, and Cloacimonadota were the most dominant in digestates regardless of the composition of the processed substrates, however, member composition at the genus level differed significantly between samples. In addition, we observed that microbial genera belonging to the less prevalent phyla play an integral role in the forming of microbial community interactions. Dominant microbial taxa with broad metabolic capabilities, potentially improving soil quality and functionality, have been identified. Moreover, we confirmed, that digestate samples were free of analyzed pathogenic bacteria and parasites. The study results indicate that digestate may have an immense fertilizing and bioremediation potential that has not been fully availed of to date.

Removal of Phosphate from Water by Iron/Calcium Oxide-Modified Biochar: Removal Mechanisms and Adsorption Modeling

Phosphorus (P) pollution is a leading cause of water eutrophication, and metal-modified biochar is an effective adsorbent with the ability to alter the migration capacity of phosphorus. This study uses bamboo as the raw material to prepare metal-modified biochar (ZFCO-BC) loaded with Fe and Ca under N2 conditions at 900 °C, and investigates its adsorption characteristics for phosphate. Batch experimental results show the adsorption capacity of the ZFCO-BC gradually increases (from 4.0 to 69.1 mg/g) as the initial phosphate concentration increases (from 2 to 900 mg/L), mainly through multilayer adsorption. Additionally, as the pH increases from 1 to 7, the adsorption capacity of the ZFCO-BC climbs to reach its maximum value of 48.4 mg/g with an initial phosphate concentration of 150 mg/L. At this pH, phosphate primarily exists as H2PO4− and HPO42−, which both readily react with Fe3+ and Ca2+ in the biochar. Furthermore, the addition of CO32−, HCO3−, NO3−, SO42−, F−, and Cl− each affect the removal rate of phosphate by less than 10%, indicating the ZFCO-BC has a highly efficient and selective phosphate adsorption capacity. A multi-column adsorption experiment designed to achieve long-term and efficient phosphorus removal treated 275.5 pore volumes (PVs) of water over 366 h. The cyclic adsorption–desorption experiment results show that 0.5 M NaOH can effectively leach phosphate from the ZFCO-BC. Observations at the molecular level from P K-edge XANES spectra confirm the removal of low-concentration phosphate is primarily dominated by electrostatic attraction, while the main removal mechanism for high-concentration phosphate is chemical precipitation. This study demonstrates that ZFCO-BC has broad application prospects for phosphate removal from wastewater and as a potential slow-release fertilizer in agriculture.

High-altitude hypoxia exposure alters follicular metabolome and oocyte developmental potential in women.

To explore the effects of high-altitude hypoxia on the microenvironment of oocyte development and fertilization potential, we compared the metabolomic patterns of follicular fluid from women living in different altitude areas and traced their oocyte maturation and subsequent development. A total of 315 clinical cases were collected and divided into three groups according to their residence altitudes: 138 cases in low-altitude (< 2300m) group, 100 cases in middle-altitude (2300-2800m) group and 77 cases in high-altitude (> 2800m) group. The clinical outcomes were statistically estimated, including hormonal level, oocyte maturation, in vitro fertilization, and embryo development. Meanwhile, a metabolomic analysis was performed on the follicular fluid of women from different groups using ultra-high-performance liquid chromatography and high-resolution mass spectrometry and differential metabolites were analyzed through the KEGG pathway. The clinical data indicated that the physical condition and reproductive hormone secretion were similar among different groups. Although personalized gonadotropin-releasing hormone strategies were applied, the numbers of antral follicles and obtained oocytes were not impacted by the residence altitude change. In in vitro culture, the maturing rate, fertility rate and cleavage rate of high-altitude group were compared with the other groups. However, the rates of high-quality embryo, formative blastocyst, and available blastocyst were gradually decreased with the rise of residence altitude. Metabolome analysis identified 1193 metabolites in female follicular fluid. Differential analysis indicated that metabolic components in follicular fluid were remarkably changed with the elevation of residence altitude. These differential metabolites were closely related with amino acid metabolism, protein digestion and absorption, oocyte meiosis and steroid biosynthesis. The residence altitude alters the microenvironment of follicular fluid, which could damage the oocyte developmental potential. This study provides diagnostic basis and therapeutic targets for research on female oocyte and embryo development.

Genetic diagnosis in XY disorders of sex development

XY disorders of sex development (XY DSD) comprise a class of heterogeneous genetic entities that result in discrepancies between chromosomal, gonadal, and phenotypic sex due to a reduction in androgen synthesis or action. The chief categories of disorders include gonadal dysgenesis, disorders with reduced androgen production (biosynthetic defect – either alone or in conjunction with impaired glucocorticoid and/or mineralocorticoid synthesis), and insensitivity to androgen action. While conventional diagnostic modalities, encompassing karyotyping, biochemistry, radiology, and, in a few cases, diagnostic laparoscopy, help in formulating a provisional diagnosis, molecular genetic testing is key to arriving at a precise etiology. Besides ending the diagnostic uncertainty, a molecular diagnosis helps to predict the natural course in terms of pubertal development and potential for fertility, thus contributing to decisions on the gender of rearing; and guides on surveillance for extragenital features and the risk of recurrence in subsequent pregnancies. This paper broadly discusses the genetic basis of XY DSD, different modalities of genetic testing, and their utility and limitations.

Use Efficiency, Reduction Potential, and Effects of Fertilizers on Carbon Emissions in China’s Major Citrus Regions

Enhancing the efficiency of fertilizer utilization and advancing fertilizer reduction efforts constitutes a pivotal initiative for augmenting the quality and productivity of the citrus industry; this constitutes an indispensable prerequisite for attaining green and sustainable development. Utilizing panel data from seven prominent mandarin-producing regions and seven prominent tangerine-producing regions in China spanning from 2002 to 2022, this study employed the stochastic frontier analysis (SFA) method to develop a translog production function model for precisely measuring the fertilizer use efficiency for mandarins and tangerines. Employing the calculated optimal fertilizer use rates, we further ascertained the fertilizer reduction potential for mandarin and tangerine; then, we estimated the associated carbon emission reduction potential within these key citrus regions. The research revealed the following findings: the overall level of citrus fertilizer use efficiency in China is comparatively low, with the mean values for mandarin and tangerine fertilizer use efficiency being merely 0.4403 and 0.3887, respectively, indicating substantial room for improvement by approximately 60%; substantial potential exists for decreasing fertilizer use in China’s citrus industry, with average reduction potentials of 66.27% for mandarins and 64.83% for tangerines, signifying a notable redundancy in fertilizer application within major citrus-producing areas. The magnitude of carbon emission reduction potential through the diminution of citrus fertilizer use is tremendous. When optimal fertilizer rates are applied, the average carbon emission reductions resulting from fertilizer reduction in mandarins and tangerines amount to 815.8681 kg/hm2 and 602.3551 kg/hm2, respectively. The average carbon reduction potential for mandarins and tangerines reach levels of 55.9673% and 61.1299%, respectively, both surpassing the threshold of 55%. Significant differences exist in the technical efficiency of fertilizer input, reduction potential, and carbon emission mitigation potential among major citrus-producing regions. Citrus orchards in Guangdong exhibit higher potential for fertilizer reduction but demonstrate a relatively low level of technical efficiency. In contrast, Hunan Province shows an opposite trend, necessitating the development of region-specific strategies. Therefore, to minimize citrus fertilizer use and augment the technical efficiency of citrus fertilizer, it is imperative to comprehensively integrate and promote the “three new” technologies aimed at reducing fertilizer use and enhancing its efficiency within the citrus industry; implement a regional coordinated development strategy for citrus fertilizer reduction; and intensify policy guidance, publicity, and training efforts related to citrus fertilizer reduction, efficiency enhancement, and carbon emissions reduction.

Supplementing the Beltsville Extender With Mitoquinol Improves the Quality and Fertility Potential of the Rooster's Cooled Sperm.

Supplementing freeze diluents with certain antioxidants can maintain the quality of chilled sperm. The present study was an attempt to investigate the effect of Beltsville extender supplementation with the mitochondrial-targeted antioxidant 'Mitoquinol' on the quality parameters and fertility potential of rooster sperm during the cooling process. Semen samples were diluted in Beltsville extender, divided into five groups, and supplemented with 0, 1, 10, 100 and 1000 nM Mitoquinol. Samples were stored at 5°C for up to 50 h and then assayed for sperm motility, viability, mitochondrial function, membrane integrity and malondialdehyde concentration after 0, 25 and 50 h of cooling. To assess reproductive performance, artificial insemination was performed using sperm cooled for 25 h. The results showed no differences between groups at the beginning time. Extender supplementation with 10 and 100 nM Mitoquinol resulted in an improvement in total motility, progressive motility, membrane integrity, mitochondrial function and viability (p ≤ 0.05), as well as a lower malondialdehyde concentration (p ≤ 0.05) in comparison to the other groups during 25 and 50 h storage. Fertility rates were higher when roosters were inseminated with semen samples supplemented with 10 and 100 nM Mitoquinol, compared to the control group. Therefore, supplementing Beltsville extender with Mitoquinol (10 and 100 nM) effective in improving the quality and fertility potential of cooled rooster sperm.