Soil Fertilization Research Articles

Evaluation of Two Ecosystem Services Provided by a Pistia stratiotes Population on the Pacific Coast of South America.

One of the most fascinating wetlands on Peru's central coast is the Santa Rosa wetland (Chancay, Lima), an ecosystem threatened by anthropogenic activities. Some of these impacts have led to the uncontrolled growth of Pistia stratiotes, an invasive aquatic plant. This study sought to quantify the regulation and provisioning of ecosystem services provided by P. stratiotes using carbon storage and the provision of biomass as indicators. To this end, the biomasses of 50 plots measuring 0.0625 m2 were weighed and georeferenced and the percentages of dry biomass (%DB) and total organic carbon in the biomass (%C) were quantified. The biomass and its coordinates were entered into ArcGIS and a Kriging interpolation technique was applied to determine the total amount of biomass (B). It was found that P. stratiotes stored 3942.57 tCO2 and that 2132.41 tons of biomass could be obtained for fodder. The total carbon stored by this aquatic plant represented 28.46% of the total carbon sequestered in the wetland ecosystem by vascular plants, suggesting that its contribution to the carbon cycle is significant. This is the first study to estimate the biomass of a floating aquatic plant population in a coastal Peruvian wetland and is a pioneering study addressing the in situ carbon estimation of Peruvian floating aquatic plants. The results and methods proposed in this research will serve in the evaluation of the potential of ecosystem services among similar populations of floating aquatic species. In addition, the data presented can be used to establish plans for the management and use of this biomass in the production of soil fertilizers and cattle forage.

Dairy effluent applications to a pasture enhance soil fertility and microbial activity without impacting soil bacterial and fungal community composition

Farm dairy effluents (FDE) from washing the milking parlor contain manure, urine, and chemicals and constitute a large amount of wastewater. Applying FDE as soil fertilizers to pastures can enhance forage yield and improve soil nutrient status. Since the dairy industry is increasingly attempting to maximize returns through better utilization of forage with lesser inputs, there is demand for a supply of FDE as fertilizers. Nevertheless, the impact of this practice on soil microbiota remains largely unexplored. It must be studied before large-scale soil disposal to avoid diminishing microbial diversity or enhancing pathogen abundance. This study evaluated the effects of applying lagoon-stored (Lagoon) and raw dairy effluents (Raw) at a rate of 50 kg N ha−1 in four equal doses, in comparison to urea fertilization, on soil fertility and the activity, abundance, and community structure of soil microbiota. Raw was obtained after solid separation, and Lagoon corresponds to the Raw stationed in a two-lagoon system. Microbial activity was assessed as basal respiration, potentially mineralizable N, potential nitrification activity, and enzymatic activities. The catabolic activity of the microbial community was evaluated using Biolog Ecoplates™. Bacterial and fungal community composition and diversity were analyzed through amplicon sequencing of 16S rRNA and ITS2. The application of FDE benefited soil fertility and microbial activity. Lagoon had the most potent effects on soil available P and extractable K+, Na+, Mg2+ and Ca2+. Soil treated with Raw displayed higher microbial activities, such as dehydrogenase, basal respiration, urease, and potentially mineralizable N, than the other treatments. FDE did not significantly alter the microbial composition, abundance, or functional diversity. In conclusion, in this short-term trial, despite changes in soil chemical properties and microbial activity, the composition and diversity of the bacterial and fungal communities remained unaffected by FDE irrigation.

基于土壤养分检测的变量施肥系统的设计与试验

In order to solve the problems of low correlation between variable fertilizer application system and soil nutrient content detection and insufficient real-time performance, a variable fertilizer application system based on real-time soil nutrient content detection was developed. This paper describes the structure, working principle and design of key components of the soil information acquisition and fertilizer application system. It includes the simulation and analysis of fertilizer application using the discrete element method and the selection of curved blade fertilizer application discs. The system uses STM32F429IGT6 microcontroller and ROS higher-level computer for decision making. The device detects soil nutrients in real time, adjusts the fertilizer motor speed accordingly, and runs autonomously along the planned path. The decision coefficient R^2 between the fertilizer application rate and the speed of the fertilizer application wheel is not less than 0.97, and the relative error between the actual fertilizer application rate and the theoretical fertilizer application rate is up to 5.91%, with the maximum value of the coefficient of variation (CV) of 10.18%. The indoor bench test shows that the relative error between the actual fertilizer application rate and the target fertilizer application rate within a single operating grid is up to 6.2%, with the maximum value of CV being 6.64%. The field test in the orchard showed that the maximum relative error between the actual fertilizer application and the target fertilizer application in a single operation grid was 6.3%, and the maximum value of CV was 12.34%, and the fertilizer application was completed in the operation grid, which demonstrated that the device was able to realize real-time and accurate variable fertilizer application according to the soil nutrient information.

Potassium supplementation mitigates flooding stress by regulating antioxidant enzymes, photosynthetic performance, yield attributes, and lint quality in cotton plants

Flooding stress declines oxygen availability in roots that induce hypoxia stress in plants, thereby severely decline plant growth. Study was undertaken to clarify how potassium supplementation alleviates flooding stress in cotton plants. We applied potassium (K) fertilizer in pot soils at different levels; K 0 = Control (No K), K 1 = 30 mg kg−1, K 2 = 60 mg kg−1 and K 3 = 80 mg kg−1 with different duration of flooding stress s. D 0 = Normal irrigation (0 day flooding), D 1 = three days flooding, D 2 = six days flooding, D 3 = nine days flooding, D4 = twelve days flooding. The K supplementation (80 mg kg−1) in soil with 3-day flooding stress showed better combination for mitigating flooding stress. The supplementation of K3 dose (80 mg kg−1) alleviated flooding against 3-day initial flooding (D1) of which physiological traits showed better response compared to prolonged flooding stress (12-day flooding stress). The result suggests that K-involving flooding stress mitigation strategy in cotton plants was active for initial flooding stress (3-day flooding stress), while it was not fully active for pronged (upto 12-day flooding stress). Furthermore, we found K significantly improved antioxidant enzymes, photosynthetic performance, yield attributes, and lint quality.

Phosphorus Availability and Balance with Long-Term Sewage Sludge and Nitrogen Fertilization in Chernozem Soil under Maize Monoculture.

A continuous long-term field experiment with maize monoculture was conducted to evaluate the P availability and balance, DM yield, P uptake, and P sorption parameters in chernozem soil after 27 years. A total of 2 doses of nitrogen (120 and 240 kg ha-1) were applied as mineral nitrogen (N120 and N240) and sewage sludge (SS120 and SS240) and compared with unfertilized control (Con). The aboveground biomass (DM) yields significantly increased in the order of Con < SS120 < SS240 < N120 < N240 treatments and the maximum P uptake was recorded for both N240 and SS240 (25.1 kg P ha-1) according to the nutrient application gradient. The N120 and N240 treatments positively influenced the DM yield but negatively influenced the P balance (-648 and -678 kg P ha-1 27 years-1), gradually bringing a risk of P deficiency in the soil. On the other hand, applications of SS120 and SS240 positively influenced the P availability and pseudototal (PAR) content in the soil, which resulted in a buildup of legacy P or an increase in P saturation greater than the environmental threshold value. Aluminum was found to be a major controlling sorption factor for P in our chernozem soil.

Effect of coating phosphorus with humic acids and micronutrients on yield of soybean and maize in succession

The increasing the efficiency of phosphate and micronutrient fertilization in tropical soils should be better studied to close crop yield gaps. The aim of this study was to evaluate the effect of a soluble P source with or without humic acids (HA) and micronutrients (M) on P-availability and maize and soybean yield in Minas Gerais State, Brazil. The crops were grown under a rainfed and no-till cropping system, with maize grown in the 2016/2017 rainy summer season, wheat grown as a cover crop in the 2017 winter season, and soybean grown in the 2017/2018 crop year. The field trial was organized in a 4 × 2 factorial arrangement [4 sources of P in combination with 2 forms of M supply]. The coated monoammonium phosphate (MAP) P source was supplied in three ways: 1) MAP, 2) MAP + HA, and 3) MAP + HA + M (B, Cu, Mn, and Zn), plus a control for P (no P). The micronutrient supply was 1) Mn + Zn + Cu + B (MZCB) and 2) control for MZCB (no M supply). Coating MAP with HA increased soil P availability but did not increase P content within the soybean leaves. The multivariate approach also showed that soybean yield can increase in response to coating MAP with HA. The study showed that MAP + HA increased soil P content. However, this increase was diminished when micronutrients were included in the same granule (MAP + HA + M). However, P sources raised P in soybean leaves equally compared to control for P. Soybean yield was unaffected by the P source, likely due to adequate leaf P concentrations across all treatments. However, soybean yield generally increased with micronutrient supply. For maize, yields were unaffected by supplying micronutrients with the P source. Conversely, maize yields typically decreased with micronutrient supply, except when using the MAP + HA + M combination granule.

Partial Substitution of Nitrogen Fertilizer with Biogas Slurry Increases Rice Yield and Fertilizer Utilization Efficiency, Enhancing Soil Fertility in the Chaohu Lake Basin.

To investigate the effects of biogas slurry substitution for fertilizer on rice yield, fertilizer utilization efficiency, and soil fertility, a field experiment was conducted on rice-wheat rotation soil in the Chaohu Lake Basin for two consecutive years, with the following six treatments: no fertilization (CK), conventional fertilization (CF), optimized fertilization (OF), biogas slurry replacing 15% of fertilizer (15% OFB), biogas slurry replacing 30% of fertilizer (30% OFB), and biogas slurry replacing 50% of fertilizer (50% OFB). The field experiment results showed that, compared with CF treatment, OF treatment in 2022 and 2023 significantly increased (p < 0.05) rice yield, promoted the uptake of nitrogen (N), phosphorus (P), and potassium (K) by grains and straws, improved fertilizer utilization efficiency, and increased the contents of soil organic C (SOC), NH4+-N, NO3--N, hydrolysable N, and available P. The 15% OFB and 30% OFB treatments significantly increased (p < 0.05) rice grain and straw yields compared with CF treatment, and rice grain and straw yields were the highest in the 30% OFB treatment. Compared with CF and OF treatments, 30% OFB treatment significantly increased (p < 0.05) the N, P, and K uptake of grains and straws and increased the fertilizer utilization efficiency. Compared with CF treatment, the grain yield of 50% OFB treatment was significantly decreased (p < 0.05) in 2022, and there was no significant difference in 2023, which may be because the biogas slurry was applied before planting in 2023 to provide more nutrients for early rice growth. Compared with CF treatment, 30% OFB treatment significantly increased (p < 0.05) the contents of SOC, NH4+-N, available K, and hydrolysable N. In summary, optimizing N and K topdressing methods can increase rice yield and improve the fertilizer utilization efficiency and soil fertility. The 30% OFB treatment resulted in the highest rice yield, fertilizer utilization efficiency, and improved soil fertility, indicating that biogas slurry replacing 30% of fertilizer was the best application mode for rice in this region.

ЭФФЕКТИВНОСТЬ ПРИМЕНЕНИЯ РАЗЛИЧНЫХ ВИДОВ УДОБРЕНИЙ ПРИ ВЫРАЩИВАНИИ КНЯЖЕНИКИ АРКТИЧЕСКОЙ (RUBUS ARCTICUS L.)

The objective of research is to develop a nutrition system for Arctic brake. The paper presents the results of research on growing Arctic brake (Rubus arcticus L.) on acidic light sod-podzolic soils in the natural and climatic conditions of the Kostroma Region. Two soil fertilization options were used: 1) azophoska 400 kg/ha (N60P60K60 as the main fertilizer + N30P30K30 top dressing); 2) the developed nutrition system, including the main organomineral fertilizer containing humic substances and bacterial spores (Bacillus subtillis, Bacillus mucilaginosus) and 3 top dressings with solutions of the mineral fertilizer Akvarin in May, June and September (10 %, 6 and 3 %, respectively). Local application of organomineral fertilizers at a dose of 500 kg/ha with 3 additional feedings of water-soluble mineral fertilizers of the Akvarin brand containing macro- and microelements has a beneficial effect on increasing the microbiological activity of the soil, ammonifiers by 25.8 %, phosphate-mobilizing bacteria by 39.1 %, its agrochemical indicators, inclu¬ding the content of organic matter by 0.4 %, mobile phosphorus and exchangeable potassium by 7.6–12.4 mg/kg, the survival rate of R. arcticus plants, the formation of the bush habitus, with the additional formation of partial shoots by 35 pcs/bush, the physiological activity of plants, expressed in an increase in the productivity of photosynthesis by 38 % and a significant increase in the yield of berries to 53.2 g/m2 (by 47.6 %). Expanding the possibility of forming R. arcticus plantations on acidic sod-podzolic soils will increase the occupancy rate of fallow soils and put them into operation. Production of berry products in the Kostroma Region as an ecologically safe region will provide enterprises engaged in the preparation of baby and dietary food with high-quality medicinal raw materials.

Agronomic Effectiveness of Zinc Sources as Micronutrient Fertilizers: A Comprehensive Review’

Zinc naturally occurs in the earth's crust as part of rocks and ore minerals. The average concentration of zinc in the lithosphere is 80 mg kg-1. While zinc is relatively immobile in soil, it is moderately mobile in plants. As a vital micronutrient for both humans and animals, zinc plays an essential role in plants as a catalytic, structural and regulatory cofactor for many enzyme reactions. It is necessary for the metabolism of carbohydrates, protein synthesis, the biosynthesis of growth hormones (particularly indoleacetic acid) and the maintenance of cell membrane integrity. Zinc deficiency is a widespread problem affecting both plants and humans in many regions of the world. To address this issue and improve plant zinc nutrition and yields, both soil and foliar zinc fertilizers have been applied. The agronomic effectiveness of Zn fertilizers has been related to the management factors such as placement, source type, seed treatment, foliar spray and biofortification that can affect the effectiveness of Zn fertilizers.

Evaluation of hydrochar-derived modifier and water-soluble fertilizer on saline soil improvement and pasture growth

Soil salinization poses a serious threat to crop growth. The selection of appropriate soil modifiers and water-soluble fertilizers for saline soils represents a crucial method for enhancing crop yields. The modifiers and medium-element water-soluble fertilizers were prepared using hydrochar derived from rice straw. Two distinct experiments were designed to study the effect of modifiers and water-soluble fertilizers on saline soils. The first experiment, designated as the “Soil Cultivation Experiment” , sought to investigate the impact of various modifiers on soil quality. The second experiment, designated as the “Method of Field Micro-Area Experiment”, aimed to assess the influence of water-soluble fertilizers on saline soils. The results showed that the application of modifiers and water-soluble fertilizers significantly enhanced comprehensive soil physical and chemical properties, crop growth, soil enzyme activity, and other key indicators in saline and alkaline soils. The optimal dosage of the modifier was 20 g/kg, which reduced the pH value from 8.62 to 8.21 and the decreased alkalinity by 8.26%. Furthermore, their application effectively boosted nutrient levels, including organic matter, and increased soil enzyme activity. The biomass of alfalfa showed enhancements of 63.01% and 20.87% and the biomass of leymus chinensis increased by 29.39% and 9.02% for the two batches, respectively. Notably, the application of water-soluble fertilizer yielded achieved superior results. This study also provided a theoretical basis for their future application in soda saline–alkali soil.

DNA barcoding and phylogenetic relationships of ecologically and commercially important seaweed species from the Azores (NE Atlantic)

Marine natural resources are increasingly demanded to support the livelihoods and wellbeing of humans facing multiple and simultaneous crises across the planet. Seaweeds, in particular, are renowned sources of bioactive marine compounds used for various purposes, including human and animal nutrition, soil fertilization, and healthcare markets. DNA-based tools are being adopted in integrative taxonomy and ecological research, as well as to monitor the supply chain of commercially important species. This research provides novel insights into the population genetics, phylogenetic relationships, and haplotype network of six commercially significant seaweed species that are abundant in the Azores archipelago and currently traded for industrial applications. This study provides a detailed assessment of the genetic diversity of the red seaweeds Asparagopsis armata, Asparagopsis taxiformis, and Pterocladiella capillacea, and the brown seaweeds Halopteris scoparia, Zonaria tournefortii, and Cystoseira pustulata, contributing with 17 newly generated barcodes of COI, rbcL, and 28S rDNA gene records from the Azores region to the reference databases. Understanding the species identification and population dynamics is critical for understanding species composition in biodiversity hotspots. This knowledge supports taxonomy, conservation, environmental protection, and the sustainable use of marine resources.

Potential of Vermifiltration Technique to Reduce Chemical Oxygen Demand, Biological Oxygen Demand, and Total Suspended Solid of Farm Dairy Effluent in Developing Countries: Case of Indonesian Farm Dairy Industry

Farm dairy effluent (FDE) contains high level of Chemical Oxygen Demand (COD), Biological Oxygen Demand (BOD), and Total Suspended Solids (TSS) which caused river and groundwater pollution. To reduce those parameters, vermifiltration technique was utilized for dairy effluent treatment. It also could reduce greenhouse gasses (GHG) emissions, removing organic and excessive nutrients without odor and recovering manure nutrients in the treated dairy effluent and vermicompost. The objective of this study is to evaluate the potential of Vermifiltration in reducing COD, BOD and TSS of the FDE. Samples of wastewater were collected from dairy farm industry from 3,542 of total cow populations located in Bandung regency, Indonesia. The sample characteristics of COD, BOD, TSS, pH, NH3-N, organic carbon, and macro nutrients (total N, P2O5, and K2O) were measured before and after vermifiltration treatment. Vermifiltration treatment will produce two types of products i.e.: tea water and vermicompost. The result showed that Vermifilter removed the COD, BOD, and TSS of 93%, 95%, and 93% respectively. The organic carbon NH3-N, and total macro nutrients generally decreased after treated by Vermifilter (2.01 to 0.0001% of organic carbon, 718 to 3.75 mg L-1 of NH3-N, 0.2 to 0.02% of total N, 0.19 to 004% P2O5, 0.57 to 0.18% of K2O); however, the pH was not significantly changing (from 8.13 to become 8.05). Vermicompost produced by vermifiltration beds by earthworms consumes sewage solids and excreting them in the vermicast which produces high macronutrients (2.4% of total N, 5.5% of P2O5, and 0.75% of K2O), and organic carbon (15.6%), hence beneficial for soil fertilizer. Vermifilter effectively degrades the organic matter in the dairy effluent. Derivatives produced by the Vermifiltration process, namely tea water and Vermicompost, can be used as substitutes for irrigation materials and organic fertilizers on agricultural land, and act as soil conditioners to improve soil properties.

Effects and mechanisms of kitchen waste organic fertilizers application on soil nitrogen transformation, plant pathogenic virulence genes, and metabolites

The application of organic fertilizers enhances soil fertility and alters the function and structure of the soil microbial communities. When subjected to aerobic composting, kitchen waste is transformed into organic fertilizer. However, the ecological benefits and safety risks of kitchen waste organic fertilizers (OF) in soil require further evaluation. Therefore, this study conducted a pot experiment to investigate the effects and mechanisms of OF application on soil properties, crop growth, nitrogen transformation, plant pathogenic virulence genes, and metabolites. The results indicated that OF treatment promoted vitamin C synthesis in pakchoi (35.23–48.00 %) and reduced soil N2O emissions during cultivation compared with inorganic fertilizer (IF) (65.50–68.08 %) (P<0.01). IF treatment significantly increased the abundance of Rhodanobacter (6.41 %) and Nitrobacter (3.16 %), leading to increased N2O emissions (17.94 mg kg−1). Key nitrogen metabolism enzymes and functional genes indicated that nitrification and denitrification pathways were the primary contributors to N2O production. Metagenomic results revealed that IF treatment enhanced virulence gene pathogenicity through quorum sensing (QS), whereas OF treatment reduced virulence gene accumulation in plant pathogenic bacteria. Metabolomic analysis demonstrated that OF treatment significantly upregulated substances beneficial to soil activity and plant growth, while inhibiting pollutant expression. PLS-PM results indicated that soil properties played a pivotal role in shaping the intricate relationships among microbial communities, functional genes, and substance metabolism. This study demonstrated that OF application contributed to the promotion of plant health and optimization of soil ecosystems, thereby providing theoretical support for the further expansion of OF applications in agriculture.

Effect of Zinc-fertilizer on varietal performance of finger millet (Eleusine coracana) and soybean (Glycine max) in western Kenya

Soil zinc deficiency in Sub-Saharan Africa (SSA) is a major contributor to poor crop responses to nitrogen, phosphorus and potassium (N, P and K) fertilizer leading to low economic returns on fertilizer use. Despite being drought-tolerant crops, finger millet (Eleusine coracana) and soybean (Glycine max) yields are consistently lower than 1000 kg ha−1 in western Kenya. On-farm trials were conducted in Bungoma and Siaya Counties of western Kenya during two subsequent cropping seasons (long & short rains of 2019) to evaluate the responses of two varieties of finger millet (U15 and SEC915) and soybean (SB19 and SB134) to N, P and K after addition of Zn. Zinc was applied at 0, 1.5, and 3 kg ha−1 and N, P and K at blanket rates. Results showed that application of Zn fertilizer alongside N, P and K fertilizer significantly (p < 0.05) increased grain yields, grain Zn concentration and grain Zn uptake. The largest Zn agronomic efficiency (AEZn) was realized when Zn was applied at 1.5 kg ha−1 for both crops. Application of Zn was profitable (VCR >1) for growing finger millet and soybean in Bungoma and Siaya during long rains and short rains. We recommend applying Zn along with N, P and K fertilizer in Zn-depleted soils to increase finger millet and soybean yields.

Effects of water and fertilizer regulation on soil microbial community, fruit nutrients, and saponin content of Panax notoginseng: A three years field experiment

In pursuit of maximum yield and economic benefits of Panax notoginseng, farmers usually apply excessive fertilizer and irrigation. Which easily induce the decline of Panax notoginseng quality, fertilizer and water use efficiencies and soil health. From 2021–2023, a field experiment was conducted in Dali Village, Luxi County, Honghe Prefecture, Yunnan Province, to study the response of soil microbial community, root nutrient content, and ginsenoside content of Panax notoginseng to different water and fertilizer regulation. The experiment including three irrigation levels (F1: 120 mm, F2: 240 mm, and F3: 360 mm) four fertilization rates(F1: 48 kg ha−1, F2: 72 kg ha−1, F2: 96 kg ha−1, and 120 kg ha−1),and a CK treatment (no fertilizer and no irrigation) The results showed that the average N, P, and K contents across all treatments were greater in 2023 than those in 2021 and 2022. W3F4 treatment significantly increased N absorption. W2F4 treatment significantly increased P and K absorption. W2F4 treatment significantly increased the Panax notoginseng yield, and W3F2 treatment significantly increased the saponin content. The Panax notoginseng yield ranged from 1165 to 3006 kg∙ha−1, with a maximum average yield in 2023. The Re content averaged 81.5 % in 2023 over 2021, and the highest Re content in W3F2 treatment in 2023 was 108.54 mg∙g−1. In 2023, W2F3 treatment had the highest R1 content of 15.34 mg∙g−1, W2F3 treatment had the highest content of Rg1 of 44.65 mg∙g−1, and W3F2 treatment had the highest Rb1 content of 52.52 mg∙g−1. From 2021–2023, the total number of unique OTUs in W1F1 was the largest, out followed by W3F2, W3F4, and W3F1. The treatment that significantly increased the bacterial Chao1 index was W3F1 and W2F2 in 2021, W3F3, W2F1, W2F1 and W2F3 in 2022, and W2F3 and W2F4 in 2023. The Shannon nor Simpson indices were not varied significantly from CK. At the phylum level, W1F3 in 2021 and W3F1 in 2022 and 2023 improved the relative abundance of Proteobacteria, Acidobacteriota At the genus level, W1F1 in 2021 and 2023 improved the relative abundance of Vicinamibacteraceae_norank and Vicinamibacterales_norank. LEfSe analysis of soil microbial communities showed the most divergent species in 2021 with W3F2, W1F1 in 2022, and W2F1 in 2023. Different water and fertilizer treatments affected the total OTU number of bacterial communities and changed the bacterial community structure. W3F1 and W2F3 treatments significantly increased soil bacterial diversity, and soil microbial diversity regulated plants N as well as R1 and Rg1 content, and the quality of Panax notoginseng is related to microbial diversity. N, P, K, and R1 had a significant positive correlation with microbial community diversity (P<0.05). The W1F1 treatment communities were relatively abundant, and the community species showed high similarity in W3F1 and W2F3.

Optimization of fertilizer use efficiency, soil quality and oil palm (Elaeis guineensis Jacq.) growth with biochar under drip irrigation conditions irrigation conditions

Biochar (BC) is an eco-friendly product characterized with high content of carbon and usually obtained by heating biomass without oxygen. Utilizing BC as organic material to amend the problematic soils and improve plant growth and yield has been proved previously. This study investigated the effect of vetiver grass biochar (VGB) on fertilizer use efficiency, soil quality, and oil palm growth performance. A net house experiment was conducted at the Farm Unit, UiTM Sarawak Branch, between August 2022 and March 2023. A factorial randomized complete block design (RCBD) with five treatments and four replications was devised. Treatments applied were: T0) absolute control; T1) 100% NPK fertilizer; T2) 100% vetiver grass biochar; T3) 50% vetiver grass biochar + 50% NPK fertilizer; and T4) 25% vetiver grass biochar + 75% NPK fertilizer. The BC application significantly improved the fertilizer use efficiency through reducing the rate of fertilizer applied. It also significantly enhanced most soil-measured chemical properties and soil nutrients. The growth performance of oil palm plant was significantly enhanced by BC application in terms of plant height, hump diameter, leaf number, chlorophyll content, and plant biomass. The BC application demonstrated its usefulness in managing soil and cultivating oil palm plant sustainably by reducing the rate of fertilizer applied and improve the fertilizer use efficiency. Based on the output, we suggest that treatment T3 (50% vetiver grass biochar + 50% NPK) can be used to improve the growth performance of oil palm.

Developments in biochar incorporated geopolymers and alkali activated materials: A systematic literature review

Annually, industries related to agricultural products generate substantial volumes of waste biomass, presenting critical challenges for solid waste management due to its environmental and health implications. Pyrolysis emerges as a significant technique for converting such waste into biochar (BC), a material with notable internal microstructure, porosity, and chemical properties, contingent on the feedstock and processing conditions. BC is recognized for its exceptional water retention and alkalinity, primarily utilized for soil enhancement and fertilization. Its application across various fields, particularly in enhancement for sustainability, has gained momentum. This review meticulously explores BC's role in sustainable composites, focusing on geopolymers and alkali-activated binders through a comprehensive examination of existing literature, underscored by bibliometric analysis. This pioneering review, leveraging the PRISMA framework and VOSviewer for bibliometric insights, aims to bridge the research gap in this evolving domain, offering a critical assessment of BC's integration in sustainable construction materials, its challenges, and future research directions. Hence, this pioneering systematic review represents the first-ever exploration within the research domain of BC's applications in geopolymers and alkali-activated materials, marking a foundational step toward understanding its potential and challenges in sustainable engineering practices.

Heavy Metal Accumulation in Soil and Plants Using Municipal Solid Waste Compost in Variable pH Conditions

ABSTRACT Municipal solid waste composts (MSWC) are extensively utilized as organic soil conditioners and fertilizers in agricultural fields across many countries. However, the use of MSWC can lead to heavy metal pollution in soil and plants, which may adversely affect plant growth. This study examined the impact of MSWC on the heavy metal content in plants and soil across various pH levels. The experiment was conducted in a pot setting with three replications. The research findings indicated that MSWC application increased the levels of copper (Cu) and zinc (Zn) in soils but had no significant effect on lead (Pb), nickel (Ni), manganese (Mn), and iron (Fe) levels. Additionally, increases were observed in the Cu, Zn, and Fe content in plants, while Pb and Ni content remained unchanged. Cu, Zn, and Fe are essential for plant growth, and their enhancement in both soil and plants is a direct result of their relatively high presence in the MSWC used. The soil-plant transfer coefficients show that the transfer of heavy metals from soil to plants is highest under acidic conditions, indicating that the heavy metal content in MSWC can vary depending on soil pH and application rate. Therefore, it is crucial to carefully analyze and evaluate the heavy metal contents of soil and compost samples before employing MSWC in agricultural fields.

Biochemical and biophysical drivers of the hydrogen isotopic composition of carbohydrates and acetogenic lipids.

The hydrogen isotopic composition (δ2H) of plant compounds is increasingly used as a hydroclimatic proxy; however, the interpretation of δ2H values is hampered by potential coeffecting biochemical and biophysical processes. Here, we studied δ2H values of water and carbohydrates in leaves and roots, and of leaf n-alkanes, in two distinct tobacco (Nicotiana sylvestris) experiments. Large differences in plant performance and biochemistry resulted from (a) soil fertilization with varying nitrogen (N) species ratios and (b) knockout-induced starch deficiency. We observed a strong 2H-enrichment in sugars and starch with a decreasing performance induced by increasing NO3-/NH4+ ratios and starch deficiency, as well as from leaves to roots. However, δ2H values of cellulose and n-alkanes were less affected. We show that relative concentrations of sugars and starch, interlinked with leaf gas exchange, shape δ2H values of carbohydrates. We thus provide insights into drivers of hydrogen isotopic composition of plant compounds and into the mechanistic modeling of plant cellulose δ2H values.

Inhibition of nitrate accumulation in vegetable by Chroococcus sp. and related mechanisms

Vegetable nitrate accumulation is a major threat to food security and human health. The application of a non-toxic and non-nitrogen-fixing cyanobacterium, Chroococcus sp., was found to reduce soil nitrate content; however, the influence of Chroococcus sp. on vegetable growth and nitrate accumulation remains unclear. In this study, Chroococcus sp. was introduced to soil fertilized with NaNO3, (NH4)2SO4, and CO(NH2)2. Variations in growth performance and nitrate content of pakchoi (Brassica chinensis L.), coupled with changes in soil fertility, were investigated through pot experiments. The varied abundance of rhizosphere bacteria and differential expression of bacterial functional genes were studied using 16S rRNA and meta-transcriptomic sequencing analyses, respectively. Chroococcus sp. reduced vegetable nitrate content by 42.29%, 21.34%, and 27.10% in pakchoi planted in soil fertilized with NaNO3, (NH4)2SO4, and CO(NH2)2, respectively. This reduction was mainly attributed to regulation of the rhizosphere bacterial community by Chroococcus sp. First, Chroococcus sp. stimulated denitrifying bacteria (such as Methylotenera, Gemmatimonas, Nitrosomonas, Nocardioides, Gaiella, Lysobacter and Sphingomonas) that contributed to a reduction in soil nitrate content. Second, Chroococcus sp. stimulated several rhizosphere bacteria such as Methylibium, Micromonospora, Bacillus, Pedomicrobium, Hyphomicrobium, Steroidobacter, Pseudolabrys, Streptomyces, Methylobacillus and Pseudomonas that directly participated in the reduction of vegetable nitrate accumulation, according to their negative correlation with vegetable nitrate content. Chroococcus sp. increased soil fertility and consequently promoted the growth of pakchoi by reducing soil salinity and increasing soil polysaccharide content, available phosphorus, and functional enzyme activity. The increased abundances of various rhizosphere bacteria genera also contributed to an increase in soil fertility and the promotion of vegetable growth. In general, this study demonstrated the effectiveness of Chroococcus sp. in reducing vegetable nitrate accumulation and explored the related mechanisms.