Fertilizer Utilization Efficiency Research Articles

The efficiency of adjusting nutrient solution renewal frequency on physicochemical properties and microbial community of cucumber exudates under closed cultivation tank.

The closed nutrient solution management method allows for the recycling and utilization of nutrient solutions, improving the efficiency of water and fertilizer utilization. This study was conducted to investigate the effects of changing the frequency of nutrient solution renewal and method of nutrient supply on the microbial communities composition, yield, and quality in closed soilless systems by using high-throughput sequencing technology and combining the physicochemical properties of root exudate solution. The results showed that different nutrient solution management modes had a significant impact on the structure and diversity of root exudate solution microbial communities. The abundance and diversity of microorganisms in inorganic perlites were correlative with EC. The abundance and diversity of bacterial communities in the root exudate solution of open liquid supply (CK) were higher than that of closed liquid supply, while the abundance and diversity of fungal communities in the root exudate solution of closed liquid supply (T1, T2, T3) were higher than that of open liquid supply. As the frequency of nutrient solution interval decreased, the accumulation of salt in root exudate solution and the richness and diversity of the fungal community also decreased, especially increasing the K+, Ca2+, and Mg2+ contents, which were positively correlated with potential beneficial Candidatus_Xiphinematobacter, Arachidicoccus, Cellvibrio, Mucilaginibacter, Taibaiella communities and decreasing the content of soluble protein, Vitamin C content, but not significantly increased cucumber yield.

Review on enhancing the efficiency of fertilizer utilization: Strategies for optimal nutrient management

Review on enhancing the efficiency of fertilizer utilization: Strategies for optimal nutrient management

Research on Underground Drip Irrigation and Soil Redistribution - Take Heshan District of Yiyang City as an Example

Abstract: The drip flow and the buried depth of drip irrigation zone are not only important parameters of subsurface irrigation design and operation management, but also important factors affecting the migration and redistribution of soil water and fertilizer. Crop growth is closely related to soil water and nitrogen leaching, water and fertilizer transport patterns and redistribution characteristics. It is of great significance to study the migration and redistribution of soil water and fertilizer under subsurface drip irrigation for improving the utilization efficiency of water and fertilizer and improving the system of subsurface drip irrigation. In this paper, the migration mode and redistribution characteristics of water and nitrogen in subsurface drip irrigation were studied and analyzed by means of laboratory soil box experiment, considering the two factors of drip flow and the buried depth of drip irrigation belt. The main research conclusions are as follows: Ⅰ. Different drip flows had effects on soil moisture front, soil moisture content and soil water redistribution. If other conditions remain unchanged, increasing the drip flow can increase the moist body area of soil and increase the moisture content of soil. The distance between vertical and horizontal directions increased with the large drip flow, and the soil water content decreased with the increase of distance. Large drip flow can promote the redistribution of soil water after drip irrigation. Ⅱ. Different drip depth had effects on soil moisture content and soil water redistribution. When the drip depth was 0cm, the surface shape of the wet body was a "horizontal" semi-ellipse after drip irrigation. When the drip depth was 10cm and 20cm, the surface of the wet body was an "upright" semi-ellipse after drip irrigation. The larger the buried depth of the drip can make the distribution of soil moisture more uniform, and the larger the buried depth of the drip can promote the migration of water in the soil. Ⅲ. Different drip discharge had an effect on soil nitrogen redistribution, and large drip discharge promoted soil nitrogen redistribution after drip irrigation. The Nitrate Nitrogen (NO3--N) content in the moist body is lower than that near the edge of the wetting front. The content of Ammonium Nitrogen (NH4+-N) near the edge of the wetting peak is higher than that inside the wetting body. Ⅳ. The redistribution of soil nitrogen was affected by different buried depth of drip head, and the greater buried depth of drip head could promote the redistribution of soil nitrogen. In terms of vertical and horizontal migration of ammonium nitrogen and nitrate nitrogen, on the day after drip irrigation, the cumulative effect of Nitrate Nitrogen (NO3--N) buried at a depth of 20cm was more significant near the wetting front edge at a larger vertical downward distance, and the cumulative effect of Nitrate Nitrogen (NO3--N) buried at a depth of 20cm was more significant inside the wetting body at a larger vertical downward distance. At the same time, the drip buried depth of 20cm can spread the cumulative effect inside the wet body and near the edge of the wet front to a longer horizontal distance.

Performance of a Drip Irrigation System under the Co-Application of Water, Fertilizer, and Air

The co-application of water, fertilizer, and air is a new water-saving irrigation method based on drip irrigation technology, which can effectively alleviate the phenomenon of soil rhizosphere hypoxia, improve water and fertilizer utilization efficiency, and inhibit the clogging of irrigation equipment in drip irrigation systems. The performance of drip irrigation systems is one of the important factors affecting the effectiveness of the co-application of water, fertilizer, and air. However, the impact of factors such as the aeration method, fertilization device, and working parameters on the performance of drip irrigation systems for the co-application of water, fertilizer, and air is still unclear. Therefore, based on two typical aeration methods, i.e., micro-nano and Venturi aeration, the performance of a drip irrigation system under the co-application of water, fertilizer, and air was studied by comparing and analyzing the effects of different aeration methods, working pressures of the drip irrigation system, and the pressure difference between the inlet and outlet of fertilizer irrigation on the spatial distribution uniformity of water, fertilizer, and air in the drip irrigation pipeline network. The results showed that the pressure difference between the inlet and outlet of fertilization irrigation had no significant impact on system performance, while the working pressure significantly affected system performance. Compared with the effective effect of Venturi aeration on system performance, micro-nano aeration can significantly affect drip irrigation system performance and effectively improve drip irrigation system performance. The micro-nano-aerated drip irrigation system with the co-application of water, fertilizer, and air under a working pressure of 0.1 MPa has better system performance. The research results are of great significance for revealing the mechanism underlying the impact of the co-application of water, fertilizer, and air on the performance of drip irrigation systems and constructing efficient drip irrigation technology for the co-application of water, fertilizer, and air.

PH-responsive chitosan nanoparticles for controlled-release nitrogen fertilizer: Template-tampering free radical graft copolymerization under energetic radiation study

Macromolecular template-tampering free radical graft copolymerization under energetic radiation is studied as a controlled process for fabricating and nanosizing chitosan-graft-polyacrylic acid interpolymer complex (CS-PAA IPC) nanoparticles. The CS-PAA IPC nanoparticles is proposed as a pH-responsive vehicle for water absorption and controlled release of nitrogen (N) fertilizer. Roles of chitosan macromolecular template and absorbed energy for controlling free radical graft copolymerization in an aqueous solution were studied. Controlling graft copolymerization, nanostructure, nanosize, and size distribution of the CS-PAA IPC nanoparticles were followed by varying different parameters, i.e., CS template chain length, AA concentrations (0.03–0.15 M), and absorbed energies (10–30 kJ/kg, kGy). The CS-PAA IPC nanoparticles have well-defined in spherical shape with the size in the range of 30–200 nm. The pre-irradiated CS template provides a crucial role for narrowing size distribution of the CS-PAA IPC nanoparticles. Kinetic swelling behavior, water absorption/diffusion mechanism, nitrogen (N) fertilizer (ammonium nitrate, NH4NO3) entrapment/controlled release based on pH-responsive characteristic were investigated in acidic to basic ranges (pH 5, 7 and 9). The diffusion mechanism of the CS-PAA IPC nanoparticles exhibited a non-Fickian diffusion. The N content was 23 g per 100 g of CS-PAA IPC nanoparticles fertilizer. Pot-testing was carried out through the baby corn growth in different soil conditions, i.e., sandy soil (SS), SS/ NH4NO3, SS/NH4NO3-entrapped CS-PAA, and cultivation soil/NH4NO3. The CS-PAA IPC nanoparticles is a promising water absorption and pH-responsive platform for N fertilizer entrapment and controlled-release for soil amending, enhancing fertilizer utilization efficiency, and reducing environmental impact due to N loss.

Controlled-Release Nitrogen Mixed with Common Nitrogen Fertilizer Can Maintain High Yield of Rapeseed and Improve Nitrogen Utilization Efficiency.

Field experiments were conducted to study the effects of different proportions of controlled-release nitrogen fertilizer mixed with quick-acting nitrogen fertilizer on the yield and nitrogen utilization efficiency of direct-seeding rapeseed. Using a conventional nitrogen application rate of 180 kg ha-1 as a control, a total of 5 types of available nitrogen fertilizers and different proportions of controlled-release nitrogen fertilizers were mixed for fertilizer treatment. The proportion of available nitrogen fertilizer used was 135 kg ha-1, and the addition ratios of the five types of controlled-release nitrogen fertilizers were 0%, 30%, 50%, 70%, and 100%, respectively (i.e., the proportion of controlled-release nitrogen to the total nitrogen application amount). These ratios were represented as N135R0, N135R1, N135R2, N135R3, and N135R4, respectively. The results showed that there was no significant difference in the number of pods per plant, the number of seeds per pod, or the grain yield under the treatment of controlled-release nitrogen fertilizer mixed with quick-acting nitrogen fertilizer for proportions of 30-50% (N135R1~R3) when compared with the control, and a stable yield was achieved. Mixing controlled-release nitrogen fertilizer under reduced nitrogen application can significantly improve the apparent utilization rate of rapeseed nitrogen fertilizer, but it first increases and then decreases with the increase of the controlled-release nitrogen mixing ratio, reaching its highest under the N135R2 treatment. The agronomic utilization efficiency and partial productivity of nitrogen fertilizer first increased and then decreased with the increased proportion of controlled-release nitrogen, and both reached their highest utilization with the N135R2 treatment. The mixed treatment of controlled-release nitrogen did not affect soil urease activity, but significantly increased soil sucrase activity. The mixed treatment of controlled-release nitrogen also increased soil microbial biomass nitrogen and carbon content. Especially in the flowering stage, the soil microbial biomass nitrogen and carbon content was significantly higher under a controlled-release nitrogen mixing ratio of 30-50%. At the same time, it had a similar effect on soil inorganic nitrogen content. Therefore, a controlled-release nitrogen mixing treatment provided sufficient nitrogen for the key growth period of rapeseed. Under the condition of reducing the amount of nitrogen fertilizer by 25% based on the amount of nitrogen fertilizer applied to conventional rapeseed, the application of controlled-release urea mixed with common nitrogen fertilizer mixed at a ratio of 30-50% can be an effective way to maintain grain yield levels and improve nitrogen utilization efficiency.

Mitigating growth-stress tradeoffs via elevated TOR signaling in rice

Rice production accounts for approximately half of the freshwater resources utilized in agriculture, resulting in greenhouse gas emissions such as methane (CH4) from flooded paddy fields. To address this challenge, environmentally friendly and cost-effective water-saving techniques have become widely adopted in rice cultivation. However, the implementation of water-saving treatments (WSTs) in paddy-field rice has been associated with a substantial yield loss of up to 50% as well as a reduction in nitrogen use efficiency (NUE). In this study, we discovered that the target of rapamycin (TOR) signaling pathway is compromised in rice under WST. Polysome profiling-coupled transcriptome sequencing (polysome-seq) analysis unveiled a substantial reduction in global translation in response to WST associated with the downregulation of TOR activity. Molecular, biochemical, and genetic analyses revealed new insights into the impact of the positive TOR-S6K-RPS6 and negative TOR-MAF1 modules on translation repression under WST. Intriguingly, ammonium exhibited a greater ability to alleviate growth constraints under WST by enhancing TOR signaling, which simultaneously promoted uptake and utilization of ammonium and nitrogen allocation. We further demonstrated that TOR modulates the ammonium transporter AMT1;1 as well as the amino acid permease APP1 and dipeptide transporter NPF7.3 at the translational level through the 5′ untranslated region. Collectively, these findings reveal that enhancing TOR signaling could mitigate rice yield penalty due to WST by regulating the processes involved in protein synthesis and NUE. Our study will contribute to the breeding of new rice varieties with increased water and fertilizer utilization efficiency.

Carbon footprint of major grain crops in the middle and lower reaches of the Yangtze River during 2011-2020

The middle and lower reaches of the Yangtze River is one of main grain production areas in China, which is of great significance to food security. Understanding the carbon footprint of major grain crop production is helpful to develop high-yield and low-carbon agriculture. Based on the data of yield, sown area and farmland production input of main grain crops (rice, wheat and maize) in six provinces (Jiangsu, Anhui, Jiangxi, Hubei, Hunan, and Zhejiang) in the middle and lower reaches of the Yangtze River from 2011 to 2020, we estimated carbon footprint in the production of the three grain crops. The results showed that from 2011 to 2020, yield per unit area, planting area, and total yield of rice, wheat and maize were the highest in Jiangsu Province. In terms of area-scaled carbon footprint, rice in the middle and lower reaches of the Yangtze River had the highest area-scaled carbon footprint, with an average of 2.0 t CE·hm-2, followed by wheat and maize. The area-scaled carbon footprint of the three staple crops was increasing. In terms of yield-scaled carbon footprint, rice was the highest, with an average of 0.8 kg CE·kg-1, followed by wheat and maize. In terms of carbon input structure, irrigation electricity, chemical fertilizers and pesticides accounted for a relatively high proportion. Irrigation electricity accounted for 35.0%, 36.3%, and 33.2% of the total carbon input of rice, wheat and maize, respectively. Chemical fertilizers accounted for 28.8%, 32.5%, and 32.5%, respectively, while pesticides accounted for 24.2%, 13.3% and 11.5%, respectively. In terms of carbon efficiency, maize had the highest (3.9 kg·kg-1 CE), followed by rice and wheat. With the green development of agriculture, carbon emission in the production of major grain crops in the middle and lower reaches of the Yangtze River could be reduced by improving irrigation efficiency, fertilizer utilization efficiency, pesticide utilization efficiency and mechanized operation efficiency, as well as diversification of straw returning, cultivation of new varieties and policy leverage.

Increasing iron use efficiency by controlling emitter clogging in drip irrigation systems

Iron is one of the essential elements for crop growth. Traditional iron fertilizer application method by injecting iron into soil results in low utilization efficiency of iron fertilizer and a considerable amount of iron residue in the soil. Applying iron fertilizer by drip irrigation system could effectively improve the utilization efficiency of iron fertilizer. However, emitter clogging seriously restrict this technology. Here, the effects of different types of iron fertilizers [Ferrous sulfate (FeSO4); Ferric citrate (FC-Fe); Sodium ferric ethylenediamine tetraacetate (EDTA-Fe); And no iron fertilizer (CK)] on emitters clogging were assessed. The components of emitter clogging substances were detected through X-ray Diffraction and Fourier Transform Infrared Spectrometer. The results showed that different types of iron fertilizers significantly affected emitter clogging. EDTA-Fe application significantly mitigated emitter clogging, reduced the total amount of clogging substances by 49.6% on average, and increased the emitter flow rate by 21.0%. This is due to EDTA-Fe chelated scaling cations (e.g., Ca2+) in water through its functional groups, thereby reducing the contents of calcite, monohydrate calcite and dolomite. However, FeSO4 and FC-Fe application aggravated emitter clogging, and increased the total clogging substances by 52.3% and 105.8%, respectively. Surprisingly, iron precipitation was not detected in the clogging substances. FeSO4 and FC-Fe aggravated clogging mainly by increasing the formation of particulate fouling (e.g., muscovite) and chemical precipitation (e.g., calcite). This study suggested choosing EDTA-Fe in drip irrigation systems to reduce emitter clogging. The research results are of significance for improving the utilization efficiency of iron fertilizer and promoting the application of iron fertilizer drip irrigation technology.

Metabolome and transcriptome analyses provide new insights into the mechanisms underlying the enhancement of medicinal component content in the roots of Acanthopanax senticosus (Rupr. et Maxim.) Harms through foliar application of zinc fertilizer.

Acanthopanax senticosus (Rupr. et Maxim.) Harms is a perennial shrub of the Acanthopanax genus in the Araliaceae family and has a high medicinal value. The application of zinc fertilizer can improve the yield and quality of medicinal materials. However, there are limited reports on approaches to increase the content of medicinal components in A. senticosus, hindering the improvement of its medicinal quality. In this study, A. senticosus was treated with 0.1% (LZn) and 0.4% (HZn) zinc sprayed on the leaf surface. The effects of zinc treatment on the medicinal components in the roots of A. senticosus were analyzed by comprehensive metabolomics and transcriptomics analyses. A total of 316 metabolites were detected, with a prevailing occurrence of terpenoids and phenylpropanoids. We identified metabolites related to the medicinal components that were upregulated after Zn treatment, including 43 terpenoids, 19 phenylpropanoids, eight phenols, and three flavonoids. Combining differential gene expression and K-means analysis, we found 95, 65, and 25 upregulated genes related to phenylpropanoid biosynthesis, terpenoid biosynthesis, and flavonoid biosynthesis, respectively. Under different concentrations of Zn treatment, the upregulated metabolite biosynthesis-related genes and differentially expressed transcription factors varied. Pearson correlation network analysis revealed significant correlations among terpenoids, phenylpropanoids, flavonoids biosynthetic genes, and several transcription factors (ERFs, WRKYs, bHLHs, NACs, and MYBs). This study lays the foundation for understanding the metabolic processes in response to varying levels of zinc foliar spray and provides a theoretical basis for enhancing the efficiency of zinc fertilizer utilization in A. senticosus.

Nitrogen (N) “supplementation, slow release, and retention” strategy improves N use efficiency via the synergistic effect of biochar, nitrogen-fixing bacteria, and dicyandiamide

Irrational nitrogen (N) fertilizer management and application practices have led to a range of ecological and environmental problems that seriously threaten food security. In this study, an effective N fertilizer management strategy was established for improving N fertilizer utilization efficiency (NUE). Biochar, N2-fixing bacteria (Enterobacter cloacae), and a nitrification inhibitor (dicyandiamide, DCD) were simultaneously added to the soil during maize cultivation. The goal was to increase soil ammonium nitrogen content and NUE by regulating the relative abundance, enzyme activity, and functional gene expression of N conversion-related soil microbes. Biochar combined with E. cloacae and DCD significantly increased soil N content, and the NUE reached 46.69 %. The relative abundance of Burkholderia and Bradyrhizobium and the activity of nitrogenase increased significantly during biological N2 fixation. Further, the abundance of the nifH gene was significantly up-regulated. The relative abundance of Sphingomonas, Pseudomonas, Nitrospira, and Castellaniella and the activities of ammonia monooxygenase and nitrate reductase decreased significantly during nitrification and denitrification. Moreover, the abundance of the genes amoA and narG was significantly down-regulated. Correlation analyses showed that the increase in soil N2 fixation and the suppression of nitrification and denitrification reactions were the key contributors to the increase in soil N content and NUE. Biochar combined with E. cloacae and DCD synergistically enabled the supplementation, slow release, and retention of N, thus providing adequate N for maize growth. Thus, the combination of biochar, E. cloacae, and DCD is effective for mitigating the irrational application of N fertilizers and reducing N pollution.

Estimation of Agronomic Characters of Wheat Based on Variable Selection and Machine Learning Algorithms

Wheat is one of the most important food crops in the world, and its high and stable yield is of great significance for ensuring food security. Timely, non-destructive, and accurate monitoring of wheat growth information is of great significance for optimizing cultivation management, improving fertilizer utilization efficiency, and improving wheat yield and quality. Different color indices and vegetation indices were calculated based on the reflectance of the wheat canopy obtained by a UAV remote sensing platform equipped with a digital camera and a hyperspectral camera. Three variable-screening algorithms, namely competitive adaptive re-weighted sampling (CARS), iteratively retains informative variables (IRIVs), and the random forest (RF) algorithm, were used to screen the acquired indices, and then three regression algorithms, namely gradient boosting decision tree (GBDT), multiple linear regression (MLR), and random forest regression (RFR), were used to construct the monitoring models of wheat aboveground biomass (AGB) and leaf nitrogen content (LNC), respectively. The results showed that the three variable-screening algorithms demonstrated different performances for different growth indicators, with the optimal variable-screening algorithm for AGB being RF and the optimal variable-screening algorithm for LNC being CARS. In addition, using different variable-screening algorithms results in more vegetation indices being selected than color indices, and it can effectively avoid autocorrelation between variables input into the model. This study indicates that constructing a model through variable-screening algorithms can reduce redundant information input into the model and achieve a better estimation of growth parameters. A suitable combination of variable-screening algorithms and regression algorithms needs to be considered when constructing models for estimating crop growth parameters in the future.

Water retention and sustained release of magnesium-based biochar modified hydrogel composite materials

In recent years, the low utilization efficiency of fertilizers and the scarcity of freshwater resources caused by globalization have received widespread attention. To address these issues, this study used an in-situ polymerization method to prepare a magnesium-based biochar modified hydrogel slow-release fertilizer (Mg-NBC/PAA) by combining magnesium-based biochar slow-release fertilizer (Mg-NBC) with polyacrylic acid hydrogel. The composition and properties of the material were characterized using various techniques, including FT-IR, SEM, XPS, and CA. The results show that the Mg-NBC successfully entered the network structure of the hydrogel material, and the hydrogel with added biochar exhibited higher water absorption performance than the pure hydrogel, with an improvement of 47.12%. Under the optimal synthesis conditions, the maximum water absorption capacity of the material was 1395.12 ± 35.45 g/g, which can increase soil water retention and holding capacity after being added to the soil. Moreover, the material also significantly promoted the growth of maize seedlings. Therefore, Mg-NBC/PAA has the potential to be widely used in sustainable agriculture, especially in dry and semi-arid areas, due to its ability to maintain soil moisture, reduce irrigation water use, and improve nutrient utilization efficiency.

Study on the effectiveness and mechanism of a sustainable dual slow-release model to improve N utilization efficiency and reduce N pollution in black soil

Long-term intensive cultivation has led to serious N loss and low N fertilizer utilization efficiency (NUE) in black soil areas. The lost N is not only a waste of resources but also a serious pollution threat to the environment, leading to the decline in water quality and food safety and the greenhouse effect. In the present study, a stable dual slow-release model, CPCS-Urea, was prepared by in situ polymerization using nitrapyrin, urea and melamine-formaldehyde resin as raw materials. The effect of the dual slow-release model was systematically evaluated using two consecutive years of field experiments. Five treatments were established in the field experiment: no N fertilizer (N0), urea (N180), 1 % CPEC-Urea, 0.5 % CPCS-Urea, and 1 % CPCS-Urea. The results showed that the new dual slow-release CPCS-Urea model outperformed both the use of urea and the traditional slow-release CPEC-Urea model in reducing N losses and improving NUE. The application of CPCS-Urea reduced nitrate (NO3−) leaching by 28.2 %–47.2 % and N2O emissions by 36.5 %–42.4 % and increased NUE by 20.7 %–28.5 % compared to urea application. The CPCS-Urea model modulated the activity of ammonia-oxidizing bacteria (AOB) and dissimilatory nitrate reduction to ammonium (DNRA) bacteria in soil, showing a significant decrease in AOB activity and an increase in DNRA activity. This results in a lower soil NO3−-N yield and a 53.1 %–72.0 % increase in NH4+-N content, providing sufficient N for the entire growth and development cycle of maize. In short, the dual slow-release CPCS-Urea model has great application prospects for promoting agricultural development in black soil areas.

Research Progress of Phosphorus Fertilizer Utilization Efficiency in Soil

Phosphorus is one of the essential macrotrophic elements in plants. It plays an important role in physiological and biological processes such as energy metabolism, nucleic acid synthesis, photosynthesis and glycolysis. Phosphate mine is also a non-renewable mineral resource, to ensure high yield crop, our country pours a large amount of phosphate fertilizer into farmland every year. Therefore, it is imperative to study the mechanism of efficient utilization of phosphorus fertilizer and the technology of reducing weight. Therefore, how to improve the utilization efficiency of phosphorus fertilizer and soil cumulative phosphorus has become a major scientific problem related to the sustainable production of crops, the realization of weight loss and efficiency and the green development of agriculture. In this paper, the research progress on the efficient utilization of phosphorus fertilizer in soil at home and abroad was reviewed. Finally, the future research directions on the efficient utilization of phosphorus fertilizer in farmland soil were prospected.

The Co-Application of PGPR and Biochar Enhances the Production Capacity of Continuous Cropping Peppers in the Karst Yellow Soil Region of Southwest China

In recent years, a significant impediment to the advancement of China’s agricultural sector is the noteworthy challenge posed by diminished crop yields and quality due to ongoing continuous cropping obstacles. Numerous studies have consistently showcased the potential of plant growth-promoting rhizobacteria (PGPR) and biochar in augmenting the alleviation of continuous cropping barriers. Nevertheless, the potential of PGPR and biochar to remediate and improve continuous cropping peppers in the karst yellow soil area remains unclear. A 2-year field experiment was implemented to examine the impact of PGPR and biochar, when applied alone or in combination, on the production potential of continuous cropping peppers. The results revealed that PGPR and biochar significantly elevated the yield of fresh and dry pepper compared with TF treatment. The utilization of PGPR and biochar resulted in an augmentation of free amino acids, soluble sugar, and vitamin C content in pepper fruits, but a reduction in the nitrate content, which proved advantageous in enhancing the overall quality of peppers. Furthermore, the use of PGPR and biochar demonstrated significant benefits in enhancing NPK accumulation, fertilizer utilization, and economic efficiency. Nevertheless, the co-application of PGPR and biochar yielded significantly better results compared to their individual application. In conclusion, the utilization of PGPR and biochar demonstrated a favorable impact on the productivity and economic benefits of continuous cropping peppers. The simultaneous application of PGPR and biochar represents a promising approach to enhancing yield and improving the quality of peppers in the karst yellow soil region of Southwest China.

Integrating the Soil Microbiota and Metabolome Reveals the Mechanism through Which Controlled Release Fertilizer Affects Sugarcane Growth.

Root-soil underground interactions mediated by soil microorganisms and metabolites are crucial for fertilizer utilization efficiency and crop growth regulation. This study employed a combined approach of soil microbial community profiling and non-targeted metabolomics to investigate the patterns of root-associated microbial aggregation and the mechanisms associated with metabolites under varying controlled-release fertilizer (CRF) application rates. The experimental treatments included five field application rates of CRF (D1: 675 kg/ha; D15: 1012.5 kg/ha; D2: 1350 kg/ha; D25: 1687.5 kg/ha; and D3: 2025 kg/ha) along with traditional fertilizer as a control (CK: 1687.5 kg/ha). The results indicated that the growth of sugarcane in the field was significantly influenced by the CRF application rate (p < 0.05). Compared with CK, the optimal field application of CRF was observed at D25, resulting in a 16.3% to 53.6% increase in sugarcane yield. Under the condition of reducing fertilizer application by 20%, D2 showed a 13.3% increase in stem yield and a 6.7% increase in sugar production. The bacterial ACE index exhibited significant differences between D25 and D1, while the Chao1 index showed significance among the D25, D1, and CK treatments. The dominant bacterial phyla in sugarcane rhizosphere aggregation included Proteobacteria, Actinobacteriota, and Acidobacteriota. Fungal phyla comprised Rozellomycota, Basidiomycota, and Ascomycota. The annotated metabolic pathways encompassed biosynthesis of secondary metabolites, carbohydrate metabolism, and lipid metabolism. Differential analysis and random forest selection identified distinctive biomarkers including Leotiomycetes, Cercospora, Anaeromyxobacter, isoleucyl-proline, and methylmalonic acid. Redundancy analysis unveiled soil pH, soil organic carbon, and available nitrogen as the primary drivers of microbial communities, while the metabolic profiles were notably influenced by the available potassium and phosphorus. The correlation heatmaps illustrated potential microbial-metabolite regulatory mechanisms under CRF application conditions. These findings underscore the significant potential of CRF in sugarcane field production, laying a theoretical foundation for sustainable development in the sugarcane industry.

Micro-nano bubble water with potassium fertigation improves strawberry yield and quality by changing soil bacterial community

Aerated drip irrigation can increase the soil oxygen content and improve the utilization efficiency of fertilizer. However, limited information is available on how soluble potassium fertilizer with different aeration levels affects plant yield and quality. Specifically, the underlying mechanism associated with microbial community level has not been reported so far. In this study, a micro-nano bubble water (MNBW) drip irrigation experiment was conducted in a strawberry plantation greenhouse, with three ratios of MNBW and two potassium application amounts and a control treatment (without MNBW). The results showed that compared with the CK (no aeration, 100% traditional potassium fertilizer application, 144 kg hm−2 of potassium sulfate), MNBW irrigation improved the soil oxygen content and changed the structure and functionality of the soil microbial community in the strawberry rhizosphere. The bacterial diversity was reduced, and the microbial co-occurrence patterns were altered. The soil respiration intensity increased by 1.32%–14.22%. The soil temperature increased, and the decomposition of organic matter was enhanced. It was indicated that the increase of soil oxygen had a positive impact on soil fertility, strawberry yield, and quality under the condition of reducing potassium application. The yield and quality of strawberries in MNBW treatments were significantly higher than those treated with CK (p < 0.05), but the carbon dioxide emissions significantly increased. High potassium fertilizer application amount and MNBW ratio did not mean high yield and quality of strawberries. Thus, O20K70 (20% of irrigation water with micro-nano bubbles, 70% of traditional drip irrigation potassium fertilizer level, 101 kg hm−2 of potassium sulfate) and O60K70 (60% of irrigation water with micro-nano bubbles, 70% of traditional drip irrigation potassium fertilizer level, 101 kg hm−2 of potassium sulfate) could be chosen for strawberry plantations with MNBW.

Effects of combined natural synergists and chemical inhibitors on yield, nitrogen utilization and balance in wheat/maize rotation system.

Urease inhibitors and nitrification inhibitors can enhance nitrogen (N) fertilizer utilization efficiency and reducing N losses through regulating urea-N transformation. Common urease or nitrification inhibitors, however, are predominantly chemically synthesized and high-cost. Furthermore, their inhibitory effects are mediated by soil pro-perties, climatic conditions, and crop systems. In this study, we conducted a field experiment using natural synergists humic acid/zeolite, along with chemical nitrification inhibitor dicyandiamide (DCD) and their combination to elucidate the impacts of natural synergists combined with chemical inhibitors on annual yield, nitrogen utilization efficiency, soil nitrate-N accumulation, and nitrogen balance within the wheat/maize rotation system. The treatments included no nitrogen fertilizer application (CK), single application of urea (N), urea +DCD (ND), urea + humic acid (NH), urea + zeolite (NP), urea + urease inhibitor N-butylthiophosphoric triamide + DCD (NUD), urea + humic acid + DCD (NHD), and urea + zeolite + DCD (NPD). The results showed that, compared to the treatments NH and NP, the integration of humic acid or zeolite with DCD (NHD and NPD) significantly increased maize yield (11268 and 11397 kg·hm-2) and total annual yield (20494 and 20582 kg·hm-2), which were comparable to those of combined chemical urease and nitrification inhibitors (NUD). The NHD and NPD treatments had higher nitrogen utilization efficiency and lower soil nitrate-N accumulation in 80-100 cm soil layer across all seasons relative to the N treatment, which had no significant difference compared to the NUD treatment. Furthermore, a decline in soil nitrogen surplus by 10.7% and 13.9% was observed when comparing the NHD and NPD treatments with the NH and NP treatments, respectively. These findings suggested that combined humic acid or zeolite and chemical nitrification inhibitors could effectively enhance crop yield and N utilization efficiency and meet the requirements of the green and environmental preservation of modern agriculture.

Effect of different NPK fertilization timing sequences management on soil-petiole system nutrient uptake and fertilizer utilization efficiency of drip irrigation cotton

In order to elucidate the effects of different nitrogen (N), phosphorus (P), and potassium (K) fertilization timing sequences management on nutrient absorption and utilization in drip irrigation cotton, field experiments were conducted from 2020 to 2021. There are six timing sequences management methods for NPK fertilization (S1–S6: 1/3Time N–1/3Time PK–1/3Time W, 1/3Time PK–1/3Time N–1/3Time W, 1/2Time NPK–1/2Time W, 1/4Time W–1/4Time N–1/4Time PK–1/4Time W, 1/3Time W–1/3Time NPK–1/3Time W), among which S6 is the current management method for field fertilization timing sequences, and S7 is the non N. The results showed that during the main growth stage, S5 accumulated more nitrate nitrogen (NO3–-N) and ammonium nitrogen (NH4+-N) content in soil between 20 and 40 cm, and accumulated more available phosphorus content in soil between 5–15 cm and 15–25 cm, S5 reducing N leaching and increasing P mobility. It is recommended to change the timing sequences management method of NPK fertilization for drip irrigation cotton to 1/4Time W–1/4Time PK–1/4Time N–1/4Time W, which is beneficial for plant nutrient absorption and utilization while reducing environmental pollution.