Fertile Individuals Research Articles

Effect of non-ionizing radiation from mobile phones in different time periods exposure on DNA integrity of infertile men

Exposure to radiation of various forms is one of the many environmental, health, and lifestyle variables that have been linked in recent years to an increase in the rate of male infertility. According to data from several recently published studies, mobile phone use may negatively affect sperm parameters, which may lower male fertility .Ninety-seven samples of semen from fertile and infertile individuals were sent to the "High Institute for Infertility Diagnosis and Assisted Reproductive Technologies. Al-Nahrain University in Baghdad, between November 2022 and May 2023. The samples were split into four groups: normozoospermia (n = 40), asthenozoospermia (n = 25), oligozoospermia (n = 16), and teratozoospermia (n = 16). Each group was further divided into two groups based on the amount of time spent using a mobile phone each day: less than 4 hours per day and equal to or more than 4 hours per day. A routine seminal fluid analysis was carried out to measure the sperm parameters and test the deoxyribonucleic acid fragmentation rate using an acridine orange stain .Samples exposed to electromagnetic waves of cell phones showed a significant decrease in DNA normality. deoxyribonucleic acid fragmentation index (p < 0.001) in normozoospermia patients exposed to more than 4 hours/day of non-ionizing electromagnetic waves. The results of deoxyribonucleic acid fragmentation index after treatment were shown a significant improvement compared to before the treatment in all fertile and infertile groups. Electromagnetic waves emitted from cell phones are associated with a decrease of deoxyribonucleic acid normality when exposure for <4hours/day. The study recommend that men of reproductive age avoid keeping their cell phones in the hip pocket and front pants pocket which may negatively affect spermatozoa deoxyribonucleic acid integrity and impair male fertility

Differences in the anatomical structure of the uterus between fertile and infertile individuals.

Infertility affects a significant portion of the reproductive population and is caused by structural and hormonal factors. The prevalence of congenital uterine anomalies is 3%-4%, with the most common anomaly being septate uterus. However, further research is needed to determine whether these anomalies can cause infertility. In this study, we compared the morphometric parameters of the uterus of fertile and infertile individuals. Based on the data obtained, we aimed to determine the parameters to be evaluated for fertility prediction and to investigate the effect of uterine septum on fertility. The uteruses of 55 infertile and 80 fertile individuals between the age range of 20-45 years were analyzed retrospectively using magnetic resonance images. Infertile individuals were categorized into two groups according to the reasons for infertility: Group I, which included women with congenital uterine anomalies (septate uterus), and Group II, which included women with tubal and male factors. Group III comprised fertile individuals. Uterine length (UL), uterine body length (UbL), cervical length (CxL), uterine cavity length (UcL), anteroposterior diameter (APD), transverse diameter (TD), fundal thickness (FT), and ostial distance (OD) were measured. The uterine positions were examined. The data of uterine variables were evaluated statistically according to age and groups. The mean ages of individuals in Groups I, II, and III were 29.88 ± 6.69, 29.21 ± 4.59, and 27.45 ± 5.43 years, respectively. Significant differences were observed between the groups in terms of UL, UbL, CxL, UcL, APD, FT, and OD variables (p < 0.05), except for TD (p > 0.05). We observed that UL, UcL, length/width ratio, and APD parameters are important factors that influence fertility. Evaluating these parameters before septum resection would be useful in predicting the contribution of this surgical operation to fertility.

Socioeconomic status and fecundability in a Danish preconception cohort.

To what extent is socioeconomic status (SES), as measured by educational attainment and household income, associated with fecundability in a cohort of Danish couples trying to conceive? In this preconception cohort, lower educational attainment and lower household income were associated with lower fecundability after adjusting for potential confounders. Approximately 15% of couples are affected by infertility. Socioeconomic disparities in health are well established. However, little is known about socioeconomic disparity and its relation to fertility. This is a cohort study of Danish females aged 18-49 years who were trying to conceive between 2007 and 2021. Information was collected via baseline and bi-monthly follow-up questionnaires for 12 months or until reported pregnancy. Overall, 10475 participants contributed 38629 menstrual cycles and 6554 pregnancies during a maximum of 12 cycles of follow-up. We used proportional probabilities regression models to estimate fecundability ratios (FRs) and 95% CIs. Compared with upper tertiary education (highest level), fecundability was substantially lower for primary and secondary school (FR: 0.73, 95% CI: 0.62-0.85), upper secondary school (FR: 0.89, 95% CI: 0.79-1.00), vocational education (FR: 0.81, 95% CI: 0.75-0.89), and lower tertiary education (FR: 0.87, 95% CI: 0.80-0.95), but not for middle tertiary education (FR: 0.98, 95% CI: 0.93-1.03). Compared with a monthly household income of >65000 DKK, fecundability was lower for household income <25000 DKK (FR: 0.78, 95% CI: 0.72-0.85), 25000-39000 DKK (FR: 0.88, 95% CI: 0.82-0.94), and 40000-65000 DKK (FR: 0.94, 95% CI: 0.88-0.99). The results did not change appreciably after adjustment for potential confounders. We used educational attainment and household income as indicators of SES. However, SES is a complex concept, and these indicators may not reflect all aspects of SES. The study recruited couples planning to conceive, including the full spectrum of fertility from less fertile to highly fertile individuals. Our results may generalize to most couples who are trying to conceive. Our results are consistent with the literature indicating well-documented inequities in health across socioeconomic groups. The associations for income were surprisingly strong considering the Danish welfare state. These results indicate that the redistributive welfare system in Denmark does not suffice to eradicate inequities in reproductive health. The study was supported by the Department of Clinical Epidemiology, Aarhus University and Aarhus University Hospital, and the National Institute of Child Health and Human Development (RO1-HD086742, R21-HD050264, and R01-HD060680). The authors declare no conflict of interest. N/A.

The Role of Anti-Sperm Antibodies in Primary, Secondary, Immunological and Idiopathic Infertility in Men

The current study aimed to conduct a statistical study to find out the numbers of men with primary and secondary infertility, immunological infertility and Idiopathic infertility in the city of Tikrit with an estimate of the levels of some sex hormones and physiological and immunological variables. The study included 400 married men who attended the Urology Department at Salah Al-Din Hospital and private clinics in the city of Tikrit and its environs for the period from April 2021 to February 2022, and their ages ranged from (20-50) years, and they were divided into two main groups as follows: It included (80) men, and it was divided into four sub-groups, namely: - The group of patients with primary infertility and included 20 individuals. The group of patients with secondary infertility included 20 individuals. And the group of immunocompromised patients included 20 individuals. And the group of patients with unexplained infertility included 20 individuals. The group of natural (fertile) individuals: It is the fifth group and represents the control group, which included (20) individuals. The results showed that the percentage of primary infertility was the highest among infertile patients, reaching 41%, while the percentage was lowest for immunological infertility, reaching 7%. While the percentage of secondary and unexplained infertility was 21% and 31%, respectively. On the other hand, the results showed that there were significant differences (P&lt;0.05) in sperm parameters between the study groups, as sperm concentrations and the percentage of mobile sperm showed a significant decrease (P&lt;0.05), especially in the secondary infertility group compared to the control group. As for the percentage of slow-moving sperms, there were no significant changes (P&lt;0.05) in all infertile groups compared with the control group. As for the percentage of non-motile sperms, it showed a significant increase (P&lt;0.05), especially in the unexplained infertility group compared with the control group. With regard to sperm shape, the percentage of deformed sperm showed a significant increase (P&lt;0.05), especially in the primary infertility group compared with the control group. The results of the current study also showed that there were significant differences (P &lt; 0.05) in the concentrations of hormones between the study groups, as the concentrations of testosterone showed a significant decrease (P &lt; 0.05), especially in the primary infertility group compared with the control group, while the least significant decrease was recorded (P&lt;0.05) in testosterone concentration in the unexplained infertility group. Regarding the prolactin hormone, a significant increase (P&lt;0.05) was recorded in the infertility groups compared with the control group, as it was significantly higher in the secondary infertility group. LH also showed significant differences (P&lt;0.05) between the study groups, and the highest significant increase was in the primary infertility group compared to the control group, while the unexplained infertility group did not show any significant differences compared to the control group. As for the FSH hormone, it showed significant differences (P&lt;0.05) between the study groups, as it recorded the highest significant increase in the primary infertility group compared to the control group, while the unexplained infertility group did not show any significant differences compared to the control group. The results of the current study also showed that there were significant differences (P&lt;0.05) in the concentrations of immunoglobulins between the study groups, as the concentrations of IgA showed a significant decrease (P&lt;0.05), especially in the secondary infertility group compared with the control group, while the least significant increase was recorded (P&lt;0.05). 0.05) in the concentration of IgA in the immunocompromised group. Regarding IgG, a significant increase (P&lt;0.05) was recorded in the infertile groups compared with the control group, if the highest significant increase was in the immunocompromised group. While the primary and secondary infertility group did not show any significant differences compared to the control group. IgM showed significant differences (P &lt; 0.05) between the study groups, and the highest significant increase was in the immunocompromised infertility group compared with the control group, while the primary and secondary infertility groups did not show any significant differences compared with the control group. Also, ASA concentrations showed a significant increase (P&lt;0.05), especially in the unexplained infertility group, while no significant change (P&lt;0.05) was recorded in the ASA concentration in the primary infertility group. With regard to white blood cells, the percentage of white blood cells showed a significant increase (P&lt;0.05), especially in the group of immunocompromised infertility, while the percentage of white blood cells was lowest in the group of primary infertility.

Telomere attrition and genomic instability in unexplained recurrent pregnancy loss in humans: A preliminary study.

Genome instability is defined as an elevated rate of DNA damage and mutations as a result of exposure to potential direct and indirect mutagens. This current investigation was designed to elucidate the genomic instability among couples experiencing unexplained recurrent pregnancy loss (uRPL). A cohort of 1272 individuals with history of unexplained RPL with normal karyotype was retrospectively screened for levels of intracellular ROS production, baseline genomic instability and telomere functionality. The experimental outcome was compared with 728 fertile control individuals. In this study, it was perceived that individuals with uRPL exhibited higher intracellular oxidative stress, along with higher basal levels of genomic instability as compared with the fertile controls. This observation elucidates the role of genomic instability as well as involvement of telomeres in cases of uRPL. It was also observed that higher oxidative stress might be associated with DNA damage and telomere dysfunction resulting in genomic instability among subjects with unexplained RPL. This study highlighted the assessment of genomic instability status in individuals experiencing uRPL.

The suppressive potential of a gene drive in populations of invasive social wasps is currently limited

Social insects are very successful invasive species, and the continued increase of global trade and transportation has exacerbated this problem. The yellow-legged hornet, Vespa velutina nigrithorax (henceforth Asian hornet), is drastically expanding its range in Western Europe. As an apex insect predator, this hornet poses a serious threat to the honey bee industry and endemic pollinators. Current suppression methods have proven too inefficient and expensive to limit its spread. Gene drives might be an effective tool to control this species, but their use has not yet been thoroughly investigated in social insects. Here, we built a model that matches the hornet’s life history and modelled the effect of different gene drive scenarios on an established invasive population. To test the broader applicability and sensitivity of the model, we also incorporated the invasive European paper wasp Polistes dominula. We find that, due to the haplodiploidy of social hymenopterans, only a gene drive targeting female fertility is promising for population control. Our results show that although a gene drive can suppress a social wasp population, it can only do so under fairly stringent gene drive-specific conditions. This is due to a combination of two factors: first, the large number of surviving offspring that social wasp colonies produce make it possible that, even with very limited formation of resistance alleles, such alleles can quickly spread and rescue the population. Second, due to social wasp life history, infertile individuals do not compete with fertile ones, allowing fertile individuals to maintain a large population size even when drive alleles are widespread. Nevertheless, continued improvements in gene drive technology may make it a promising method for the control of invasive social insects in the future.

Comparative analysis of growth, survival and sex proportion among tetraploid-based autotriploid (Crassostrea gigas and C. angulata) and their allotriploid oysters

Triploid is widely cultivated in China and many other oyster-producing countries for the advantages of fast growth, favorable infertility and better meat quality. However, triploid Crassostrea gigas is found to have disadvantages of slow growth in later grow-out stage and high summer mortality, while the triploid C. angulata has the disadvantages of fragile shells and a relatively high ratio of developed gonads in population, and therefore, both of them have space for further improvement. Due to the limitations of poor fertility and low survival of offspring, triploid genetic improvement cannot be carried out directly. Hybridization between species is a useful method for indirect genetic improvement of triploids, and natural crosses between tetraploid males and diploid females have proven to be the best method for commercial promotion of triploids. The growth, survival and gonad development of autotriploids (DGTG-diploid C. gigas ♀ × tetraploid C. gigas ♂, DATA-diploid C. angulata ♀ × tetraploid C. angulata ♂) and allotriploids (DATG-diploid C. angulata ♀ × tetraploid C. gigas ♂, DGTA-diploid C. gigas ♀ × tetraploid C. angulata ♂) were investigated in this study. It is showed that the fertilization rates of autotriploids were significantly higher than that of allotriploids, and DATG larvae grew significantly faster than the other three groups (P < 0.05). DGTA had significant shell height, whole weight and survival advantages at Laizhou site (P < 0.05), while DATG exhibited significantly fastest growth and highest survival at Zhangpu site (P < 0.05), but both DATG and DATA showed significantly faster growth and higher survival at Beihai site (P < 0.05), with no significant difference between them (P > 0.05). Sites, genotypes and their interaction always presented significant effects on shell height and whole weight from the 90th to 360th day, but not on survival with the 180th day as an exception. Gonad examination confirmed that the proportions of fertile individuals in the groups with maternal C. gigas (DGTG and DGTA) were lower than that in the groups with maternal C. angulata (DATA and DATG), and DATA always had the highest rate of developed gonad at three sites. These results indicate that DGTA has advantages in growth, survival and infertility in northern China while DATG performs best in southern China, which exhibits that there is a significant maternal effect of hybridization in allotriploids, and promise a guidance for their commercial production.

HLA sharing and maternal HLA expression in couples with recurrent pregnancy loss in Taiwan

ObjectiveThe aim of this study is to investigate the frequency and distribution of human HLA sharing and maternal HLA allele expression in couples with recurrent pregnancy loss in Taiwan. Materials and methodsWe retrospectively reviewed couples experienced two or more pregnancy loss before 20th weeks of gestation from March 2014 to November 2020 having HLA determination. Fertile individuals with one or more live-birth offspring receiving HLA allele determination during the same period were included as the control group. The distribution and frequency of HLA sharing were analyzed and presented by descriptive statistics. Fisher Exact Test were used to analyze specific maternal and paternal HLA allele comparing individuals with RPL to fertile group. P-value < 0.05 was thought to be statistically significant. Results72 couples were enrolled from March 2014 to November 2020. Regarding the distribution of HLA sharing, HLA sharing between females and their male partners less and equal to 2 pairs were found in 40.3% of the couples. HLA sharing greater and equal to 3 pairs are found in 59.8% couples. HLA sharing was most frequently found in alleles HLA-A02, A11, DQ07, C07 and B60 in descending order. There was a significant lower expression of HLA-B13 in women with RPL compared to women who had successful pregnancy (p = 0.0234). Compared infertile men with fertile men cohort, the frequency of HLA-DR04 (p = 0.0438, OR 2.444, 95% CI 1.0251–5.8287), HLA-DR12 (p = 0.001, OR 30.85, 95% CI 4.0296–236.19) and HLA-15 (p = 0.0357, OR 9.354, 95% CI 1.1610–75.37) were found to be significantly higher in men with RPL. On the contrary, HLA-DR07 (p = 0.0085, OR 0.124, 95% CI 0.0264–0.587) and HLA-DR10 (p = 0.0395, OR 0.048, 95% CI 0.0027–0.8641) were found to be significantly lower in men with RPL. ConclusionWe found a tendency to recurrent pregnancy loss in couples with more than 2 pairs of HLA sharing. The similarity of HLA sharing, the expression of maternal HLA-B13 allele and paternal HLA-DR alleles in Taiwanese couples might play a role in recurrent pregnancy loss.

P-524 Bi-allelic CFAP61 variants cause male infertility in humans and mice with severe oligoasthenoteratozoospermia

Abstract Study question Are mutations in cilia and flagella-associated protein 61 (CFAP61) associated with human male infertility? Summary answer Bi-allelic variants ([NM_015585.4: c.1654C&amp;gt;T (p.R552C) and c.2911G&amp;gt;A (p.D971N), c.144-2A&amp;gt;G and c.1666G&amp;gt;A (p.G556R)] in CFAP61 were identified as contributory genetics factor in severe oligoasthenoteratozoospermia (OAT). What is known already Cfap61 knockout mice were infertile due to multiple morphological abnormalities of the sperm flagella (MMAF). However, so far there is no direct evidence that mutations of CFAP61 cause OAT and male infertility. Study design, size, duration Variant screening was performed by whole-exome sequencing (WES) from 325 infertile patients with OAT and 392 fertile individuals. A knockout mouse model was generate to confirm the candidate disease-causing gene, intracytoplasmic sperm injection (ICSI) was used to evaluate the efficiency of clinical treatment. Participants/materials, setting, methods A total 325 OAT-affected patients and 392 men with normal fertility were recruited from China. WES was performed, followed by Sanger sequencing validation. In silico bioinformatics predictions and in vitro functional analyses were performed to evaluate the impacts of candidate disease-causing variants. Hematoxylin and eosin (H&amp;E) staining, electron microscopy, and immunofluorescence assays were performed to evaluate the sperm morphology. Two OAT-affected men with CFAP61 variants were treated by ICSI, and pregnancy outcomes were followed. Main results and the role of chance We identified bi-allelic CFAP61 variants [NM_015585.4: c.1654C&amp;gt;T (p.R552C) and c.2911G&amp;gt;A (p.D971N), c.144-2A&amp;gt;G and c.1666G&amp;gt;A (p.G556R)] in two (0.62%) of the 325 OAT-affected men. In silico bioinformatics analysis predicted that all four variants were deleterious, and in vitro functional analysis confirmed the deleterious effects of the mutants. Notably, H&amp;E staining and electron microscopy analyses of the spermatozoa revealed multiple morphological abnormalities of sperm flagella, the absence of central pair microtubules, and mitochondrial sheath malformation in sperm flagella from man with CFAP61 variants. Further immunofluorescence assays revealed markedly reduced CFAP61 staining in the sperm flagella. In addition, Cfap61-deficient mice showed the OAT phenotype, suggesting that loss of function of CFAP61 was the cause of OAT. Two individuals accepted ICSI therapy using their own ejaculated sperm, and one of them succeeded in fathering a healthy baby. Limitations, reasons for caution Limitations include the lack of in vivo data from the one of patients, and the exact molecular mechanism should be further investigated. Wider implications of the findings Our findings indicate that CFAP61 is essential for spermatogenesis and that bi-allelic CFAP61 variants lead to OAT and male infertility in humans and mice. In addition, our results show that ICSI treatment can be recommended for CFAP61-related OAT. Trial registration number not applicable

Home Alone: Exploring Childcare Options to Remove Barriers to Second Childbearing in Belarus

This study investigates the relationship between childcare usage and parents’ intentions to have a second child in Belarus. Previous research has established that low fertility in Belarus can be primarily explained by falling second birth rates. However, a substantial research gap remains regarding the determinants of the low rate of second childbearing in Belarus. Based on a comprehensive review of hypothesised fertility barriers and family policy options in Belarus, this study leverages data from the Belarusian Generations and Gender Survey (GGS) from 2017 to examine the relationship between formal, informal, and mixed childcare usage and parents’ intention to have a second child. The analysis is based on fertile individuals aged 18–45 who have a partner and one biological child under 11 years old (i.e., up to the age at which children leave primary school). The model controls for sex, age, education, respondents’ economic wellbeing, the employment status of both partners, and the age of their child. Applying logistic regression, the analysis demonstrates that mixed childcare support increases respondents’ intentions to have an additional child. Having a child aged 3–6 years, being below 26 years old and male, are also associated with a higher likelihood of intentions to have a second child. No association was found between economic wellbeing or employment status and second‐parity fertility intentions. The results of this study suggest that gender‐egalitarian family policy instruments that improve institutional childcare and that incentivise men to participate in childcare could reduce barriers to second childbearing in Belarus.

The microbiome of seminal fluid

Manole COJOCARU Department of Physiology and Immunology, “Titu Maiorescu” University, Faculty of Medicine, Bucharest, Romania Abstract The microbiome of semen is a field of increasing scientific interest. Compared to other body sites, the seminal microbiota has been minimally investigated. Recent studies indicate that presence of bacteria in semen is relatively frequent, including in fertile individuals

Fine mapping of Rf2, a minor Restorer-of-fertility (Rf) gene for cytoplasmic male sterility in chili pepper G164 (Capsicum annuum L.).

Genome re-sequencing and recombination analyses identified Capana06g000193 as a strong candidate for the minor male fertility restoration locus Rf2 in chili pepper G164 harboring two dominant male fertility restoration genes. Male fertility restoration genes of chili pepper restorer line G164 (Capsicum annuum L.) were studied using molecular marker genotypes of an F2 population (7G) of G164 crossed with the cytoplasmic male sterility line 77013A. The ratio of sterile to fertile single plants in the F2 population was 1:15. This result indicates that chili pepper G164 has two dominant restoration genes, which we designated as Rf1 and Rf2. An individual plant recessive for Rf1 and heterozygous for Rf2, 7G-112 (rf1rf1Rf2rf2), was identified by molecular marker selection and genetic analysis, and a single Rf2 gene-segregating population with a 3:1 ratio of fertile to sterile plants was developed from the self-pollination of male fertile individuals of 77013A and 7G-112 hybrid progeny. Bulk segregant analysis of fertile and sterile pools from the segregating populations was used to genetically map Rf2 to a 3.1-Mb region on chromosome 6. Rf2 was further narrowed to a 179.3-kb interval through recombination analysis of molecular markers and obtained the most likely candidate gene, Capana06g000193.

Sperm centriole assessment identifies male factor infertility in couples with unexplained infertility – a pilot study

Unexplained infertility affects about one-third of infertile couples and is defined as the failure to identify the cause of infertility despite extensive evaluation of the male and female partners. Therefore, there is a need for a multiparametric approach to study sperm function. Recently, we developed a Fluorescence-Based Ratiometric Analysis of Sperm Centrioles (FRAC) assay to determine sperm centriole quality. Here, we perform a pilot study of sperm from 10 fertile men and 10 men in couples with unexplained infertility, using three centriolar biomarkers measured at three sperm locations from two sperm fractions, representing high and low sperm quality. We found that FRAC can identify men from couples with unexplained infertility as the likely source of infertility. Higher quality fractions from 10 fertile individuals were the reference population. All 180 studied FRAC values in the 10 fertile individuals fell within the reference population range. Eleven of the 180 studied FRAC values in the 10 infertile patients were outliers beyond the 95% confidence intervals (P = 0.0008). Three men with unexplained infertility had outlier FRAC values in their higher quality sperm fraction, while four had outlier FRAC values in their lower quality sperm fraction (3/10 and 4/10, P = 0.060 and P = 0.025, respectively), suggesting that these four individuals are infertile due, in part, to centriolar defects. We propose that a larger scale study should be performed to determine the ability of FRAC to identify male factor infertility and its potential contribution to sperm multiparametric analysis.

Characteristics of miRNAs Present in Bovine Sperm and Associations With Differences in Fertility.

Small non-coding RNAs have been linked to different phenotypes in bovine sperm, however attempts to identify sperm-borne molecular biomarkers of male fertility have thus far failed to identify a robust profile of expressed miRNAs related to fertility. We hypothesized that some differences in bull fertility may be reflected in the levels of different miRNAs in sperm. To explore such differences in fertility that are not due to differences in visible metrics of sperm quality, we employed Next Generation Sequencing to compare the miRNA populations in Bos taurus sperm from bulls with comparable motility and morphology but varying Sire Conception Rates. We identified the most abundant miRNAs in both populations (miRs -34b-3p; -100-5p; -191-5p; -30d-4p; -21-5p) and evaluated differences in the overall levels and specific patterns of isomiR expression. We also explored correlations between specific pairs of miRNAs in each population and identified 10 distinct pairs of miRNAs that were positively correlated in bulls with higher fertility and negatively correlated in comparatively less fertile individuals. Furthermore, 8 additional miRNA pairs demonstrated the opposite trend; negatively correlated in high fertility animals and positively correlated in less fertile bulls. Finally, we performed pathway analysis to identify potential roles of miRNAs present in bull sperm in the regulation of specific genes that impact spermatogenesis and embryo development. Together, these results present a comprehensive picture of the bovine sperm miRNAome that suggests multiple potential roles in fertility.

Molecular signature of immunological mechanism behind impaired endometrial receptivity in polycystic ovarian syndrome.

Despite the treatment of anovulation, infertility is still one of the main complications in PCOS women during reproductive age, which appears to be mainly due to impaired uterine receptivity. This study investigated the transcriptome profiles of endometrium in PCOS patients and healthy fertile individuals as the control group. Total mRNA was extracted from endometrial tissues of PCOS patients (n = 12) and healthy fertile individuals (n = 10) during the luteal phase. After cDNA synthesis, PCR array was performed using Human Female Infertility RT2 Profiler PCR Array kit (Qiagen, Cat.No: PAHS-164Z) for evaluating expression of 84 genes contributing to the female infertility. PCR Array data analysis identified significantly greater expression of CSF, IL11, IL15, IL1r1, IL1b, TNF, LIF, TNFRSF10B, TGFβ, C3, ITGA4 (Cd49d), SPP1, and Calca in PCOS women than in controls (P < 0.05). However, the expression of LIFR, C2, CD55, CFD, CALCA, LAM1, LAMC2, MMP2, MMP7, MMP9, ESR, SELL, ITGB3, and VCAM1 was significantly lower in PCOS group than in controls (P < 0.05). The results revealed dysregulation of immune-inflammatory molecules, complement activation and downregulation of IGF-I as well as adhesion molecules in PCOS group. The findings of this study indicated some potential causes of reduced receptivity of endometrium thus compromising the fertility in PCOS patients.

Evolution of the World Health Organization semen analysis manual: where are we?

The WHO (World Health Organization) manuals provide state-of-the-art guidance on how a semen analysis should be carried out. The much anticipated sixth edition of the WHO semen analysis manual has been released 10 years after its previous version and includes essential updates, such as new reference standards for semen volume and microscopic sperm characteristics of recent fathers. A well-conducted semen analysis remains an essential foundation of the infertility evaluation process and affects patient referral, diagnosis and treatment. However, a male infertility work-up primarily based on routine semen analysis does not provide men with an optimal fertility pathway; the primary reasons for routine semen analysis inadequacy in this context include its low predictive value for natural and assisted conception success, its inability to detect sperm DNA and epigenetic deficiencies that might negatively affect embryo development, implantation and offspring well-being, and the substantial overlap between semen parameters of fertile and subfertile individuals. Ideally, a full andrological assessment should be carried out by reproductive urologists in all men dealing with couple infertility and should include a detailed history analysis, physical examination, semen analysis, endocrine assessment and other tests as needed. Only through a complete male infertility work-up will relevant underlying medical and infertility conditions be revealed and potentially treated or alleviated. The ultimate goals of a comprehensive andrological assessment are to positively influence overall male health, pregnancy prospects and offspring well-being.

Evaluation the expression of insulin and insulin receptor-beta (IR-β) in sperm of infertile male with failed intracytoplasmic sperm injection (ICSI)

Introduction: Most infertility treatment centers are currently using semen analysis to differentiate between fertile and infertile individuals. Recent studies have evaluated the expression and secretion of independent insulin and related beta receptor (IR-β) in mammals’ ejaculated spermatozoa such as humans and pigs, and its effect on motility parameters capacitation and acrosomal reaction. Objectives: This study aimed to investigate the expression of insulin, IR-β and the parameters of sperm and DNA fragmentation index (DFI) in men with intracytoplasmic sperm injections (ICSI) failed. Patients and Methods: This study was conducted on 15 men with unknown infertility with a history of unsuccessful ICSI and 10 fertile men. After collecting samples, sperm parameters (concentration and motility), sperm DNA fragmentation and expression of insulin and IR-β genes were investigated. To assess DFI from SDFA kit and to review the expression, the genes were analyzed via RT-PCR method. Data were analyzed statistically by t-test and covariance tests. Results: Based on the findings of this research, the concentration and percentage of motility in infertile men with a history of unsuccessful ICSI was significantly lower than fertile individuals, moreover, the amount of DNA fragmentation significantly increased in infertile men with a history of unsuccessful ICSI compared to fertile men (P &lt; 0.05). Insulin expression in the sperm of infertile men with a failed ICSI history was significantly lower than fertile men (P &lt; 0.05); however, the IR-β expression rate was not significantly different between the two groups. Conclusion: Evaluation of insulin gene expression can be helpful as a good marker for identifying infertility with an unknown cause.

Biallelic CFAP61 variants cause male infertility in humans and mice with severe oligoasthenoteratozoospermia

BackgroundThe genetic causes for most male infertility due to severe oligoasthenoteratozoospermia (OAT) remain unclear.ObjectiveTo identify the genetic cause of male infertility characterised by OAT.MethodsVariant screening was performed by whole-exome sequencing...

Human live spermatozoa morphology assessment using digital holographic microscopy

Digital holographic microscopy (DHM) was applied for the morphological assessment of live intact spermatozoa from fertile and infertile men directly after semen liquefaction. This method allowed us to study the sperm population directly from the sample droplet and not only from the focal plane of the microscope as in classical optical microscopy. The newly implemented 3-dimensional sperm morphological parameters (head height, acrosome/nucleus height, head/midpiece height) were included in morphological assessment of semen samples from fertile and infertile individuals. The values of the 3D parameters were less variable in fertile men than for infertile ones. DHM was also used to compare the morphological profiles of spermatozoa after applying the “swim-up” and gradient centrifugation techniques. During selection, the most statistically significant differences were observed after separation with a Percoll gradient of 90% and a 60-min “swim-up” procedure versus ‘native’ unfractionated samples. This shows that the developed methodology can be efficiently used for the selection of morphologically sound spermatozoa. The motility type for each spermatozoon was also assessed. The results indicate that the extension of the number of morphological parameters with new 3D parameters and the simultaneous assessment of sperm motility may be valuable addition to sperm examination.

Fine-Scale Analysis of Runs of Homozygosity Islands Affecting Fertility in Mares.

The loss of genetic variability in livestock populations bred under strict selection processes is a growing concern, as it may lead to increased inbreeding values and lower fertility, as a consequence of the “inbreeding depression” effect. This is particularly important in horses, where inbreeding levels tend to rise as individuals become more and more closely related. In this study, we evaluated the effect of increased inbreeding levels on mare fertility by combining an SNP-based genomic approach using runs of homozygosity and the estimation of genetic breeding values for reproductive traits in a large population of Pura Raza Española mares. Our results showed a negative correlation between whole-genome homozygosity and fertility estimated breeding values (EBVs) at the genome level (ρ = −0.144). However, the analysis at chromosome level revealed a wide variability, with some chromosomes showing higher correlations than others. Interestingly, the correlation was stronger (−0.241) when we repeated the analysis in a reduced dataset including the 10% most and least fertile individuals, where the latter showed an increase in average inbreeding values (FROH) of around 30%. We also found 41 genomic regions (ROHi, runs of homozygosity islands) where homozygosity increased 100-fold, 13 of which were significantly associated with fertility after cross-validation. These regions encompassed 17 candidate genes previously related to oocyte and embryo development in several species. Overall, we demonstrated the relationship between increased homozygosis at the genomic level and fertility in mares. Our findings may help to deal with the occurrence of inbreeding depression, as well as further our understanding of the mechanisms underlying fertility in mares.