A study was conducted in an agricultural field to examine the biomass production of three fast-growing short rotation woody crop (SRWC) species, Populus deltoides, Quercus pagoda, and Platanus occidentalis using fertilization and irrigation (fertigation). The study included a randomized complete block (RCB) with five treatments; control, irrigated, and irrigated with 56, 112, and 224 kg nitrogen (N) ha−1 year−1. We quantified survival, basal area, standing biomass, aboveground net primary productivity (ANPP), leaf area index (LAI), and growth efficiency (GE) for each species along the soil nitrogen and water gradient. P. deltoides had low rates of survival (83, 82, and 77% years six, seven, and eight, respectively), but had production values greater than Q. pagoda and P. occidentalis. Standing biomass reached its peak for P. deltoides and P. occidentalis (17.56 and 10.36 Mg ha1, respectively) in the irrigation treatment, and in the 112 kg N treatment for Q. pagoda (5.42 Mg ha−1). P. deltoides and P. occidentalis ANPP peaked in the irrigation treatment (6.66 and 6.31 Mg ha−1 year−1, respectively) and in the 112 kg N (4.43 Mg ha−1 year−1) for Q. pagoda. ANPP was correlated with LAI; however, the relationship was species specific. Maximum ANPP was reached below the maximum LAI for Q. pagoda and P. occidentalis. P. deltoides ANPP was highest at the maximum LAI, which was achieved with IRR. These results suggest that species-specific cultural practices producing optimum LAI and maximum ANPP should be identified before fertigation techniques are adopted widely for SRWC production on agricultural fields.