Ferric Reducing Antioxidant Potential Research Articles

Formulation of Polyherbal Carbonated Beverage Based on Halalan Thoyyiban Principle

Herbal products usually have less palatability on their taste even though they are healthy and nutritious. Thus, this study aims to formulate polyherbal carbonated beverages, followed by the evaluation of sensory, physicochemical properties and their compliances with the halalan thoyyiban principle. Five formulations were prepared by varying the amount of water and polyherbal extracts that were designated using Design Expert 6.0.4 software. The most accepted formulation was formulation 1 (F1), containing 12.50% polyherbal extract and 87.50% water. F1 was characterised for its physicochemical properties, including carbon dioxide volume, pH, and total acidity, with 3.0 g/L, 4.5, and 1.7 %, respectively, and these findings have complied with the halalan thoyyiban principle. The antioxidant properties were evaluated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant potential (FRAP), as well as total phenolic content (TPC), and it exhibited 1.25 mg GAE/mL,1.08 mM Fe (II) and 0.39 mg GAE/mL respectively. Eight Halal Control Point (HCP) were identified along with the production. Moreover, the beverage was found to have antioxidant properties and nutritional content (carbohydrate (0.3%) and energy content (2.0 kcal/100g)), which can give health benefits to the consumer and proven safe for consumption (LC50 =1066.60 μg/ mL). To conclude, the formulated polyherbal carbonated beverage is accepted by the consumer and complies with the halalan thoyyiban principle.

In-Vitro Antioxidant Activites of Novel Synthesized Copper (II) Complexes

Introduction: The term radical is originally implied, to a portion of a molecule which cannot exist independently in nature. The Development of these radicals is driven by thermal hemolysis, high energy radiation and photolysis. Free radical attacks cause cell damage and homeostatic disruption. Targets of free radicals are all kinds of molecules in the body, such as lipids, nucleic acids and proteins are the major targets. Antioxidants are substances capable of slowing or stopping oxidation pathways that occur under the effect of reactive oxygen moieties or free atmospheric oxygen. Objectives: The main objective of the present study was to judge the advantages of the newly synthesized Copper (II) complexes in contrast to oxidative stress caused by free radicals utilizing different assays. Methods: The antioxidant ability of Cu (II) complexes were measured employing different assays including, DPPH free radical scavenging ability, ferric reducing antioxidant potential (FRAF) and total antioxidant ability assay. Results: In the DPPH scavenging potential assay, ferric reducing antioxidant potential and total antioxidant ability assay, RI-1 complex was studied to be more spectacular among the copper(II) complexes. Inhibiting ability of the complexes was RI-1 > NM-3 > AM-2 > SR-3. The Antioxidant scavenging potential was conformed as dose dependent which increases with the increase of complex concentration. Conclusion: The Copper (II) complexes were found to have high antioxidant abilities.

Effects of L.plantarum dy-1 fermentation time on the characteristic structure and antioxidant activity of barley β-glucan in vitro

This article explored the effect of Lactobacillus plantarum dy-1 (L. plantarum dy-1) fermentation on the basic physicochemical properties and associated in vitro antioxidant activity of barley β-glucan, including its molecular weight, monosaccharide composition, characteristic structure and rheology. Its DPPH, ABTS, hydroxyl radical scavenging capacity, and ferric reducing antioxidant potential (FRAP) were measured at different fermentation times. The results showed that the molecular weight of barley β-glucan was decreased from 1.052 × 105 Da to 4.965 × 104 Da within 0–24 h by L. plantarum dy-1 fermentation, but there was no effect on its characteristic structure. The water- and oil-holding properties of barley β-glucan were significantly enhanced with increased fermentation time, and the fluid viscous behavior of barley β-glucan was enhanced at 6% concentration, while elastic characteristics were weakened. The fermentation had no significant effect on the scavenging effect of DPPH and ABTS radicals of barley β-glucan, but the hydroxyl radical scavenging activity and total antioxidant capacity of FRAP were enhanced with increased fermentation time. Fermentation time may change the physicochemical properties and enhance antioxidant activity of barley β-glucan by reducing its molecular weight.

Phytochemical Screening, gc-ms Analysis and Antioxidant Activity of Methanol Stem Bark Extract of Cassia Sieberiana DC

The over production of free radicals in the human body is known to cause oxidative stress which plays an important role in the pathogenesis of many diseases. Therefore, the search for natural antioxidants would continue to be a major area of research for many decades. This study was to investigate the in vitro antioxidant activities and to determine the phytochemicals present in Cassia sieberiana. The methanol stem bark extract of C. sieberiana was subjected to phytochemical screening and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Its antioxidant activities were assayed in vitro using nitric oxide scavenging assay, ferric reducing antioxidant potential assay, lipid peroxidation inhibitory assay and 2-diphenyl-1-picrylhydrazyl scavenging assay. The phytochemical screening of the stem bark extract of C. sieberiana showed saponins, alkaloids, flavonoids, steroids, tannins, phlobatannins, terpernoids, cardiac glycosides, phenolics and reducing sugar. The GC-MS Table revealed that the methanol extract has 51 bioactive compounds with 3-0-Methyl-d-glucose having the highest percentage of 12.16 % while 5.alpha-Androstan-3-beta-ol,4,4-dimethyl- having the lowest percentage of 0.09 %. The antioxidant study showed concentration-dependent radical scavenging activity of the methanol stem bark extract of C. sieberiana. This concentration-dependent result was also observed when compared with ascorbic acid used as the standard drug. The presence of different phytochemicals such as alkaloids, saponins, tannins, phenols, flavonoids, among other things, might be responsible for C. sieberiana antioxidant activity. The study concluded that the methanol stem bark extract of C. sieberiana contains 51 bioactive compounds and showed good antioxidant activity which could be therapeutic in several human diseases. Key words: Cassia sieberiana, Phytochemical, Antioxidant, Pharmaceuticals

Antibacterial Activity, Antioxidant Potential and Stigmasterol Isolation from Laggera aurita Linn (Asteraceae)

The study was aimed at the in-vitro investigation of the antibacterial activity, antioxidant potential and bioactive compound isolation from ethyl acetate crude fraction of Laggera aurita (L. aurita) Linn. The crude fraction was tested against five gram negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhi, Proteus mirabilis) and three gram positive bacteria (Staphylococcus aureus, Enterococcus faecalis and Bacillus subtilis) using macro dilution technique. The antioxidant potentials were evaluated using two different but complementary methods namely ferrous ion chelating activity (FICA) and ferric reducing antioxidant potential (FRAP). Phytocompound isolation was carried out on low pressure open column chromatography. The crude fraction displayed moderate to significant activity against all tested bacteria exhibiting both bacteriostatic and bactericidal effects. The crude fraction showed Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) in the range of 62.50-250 µg/mL and 125-1000 µg/mL respectively. The crude fraction was bactericidal to all tested pathogens except E. coli. The fifty percent effective concentration (EC50) of EDTA standard and the ethyl acetate fraction were obtained as 10.87 μg/mL and 25.77 μg/mL respectively. Similarly the FRAP determinations for ascorbic acid and ethyl acetate fraction yielded 153.63 and 134.40 Fe2+ Equivalent per g of fraction indicating 80.06% and 70.61% FRAP units respectively. The isolated phytocompound coded LAE was obtained as a white crystalline solid with melting point of 136‐138°C and Rf of 0.56 in hexane: ethylacetate (6:4). The isolated compound was identified by spectroscopic data analysis from FT-IR, GC-MS, 1D and 2D NMR and in comparison with literature. The compound was identified as stigmasterol. The observed significant antibacterial and antioxidant properties demonstrated by L. aurita in this study validate its widespread use in traditional medicine. Therefore the study had shown that L. aurita contains bioactive principles and may serve as a source for potential antibacterial and oxidative stress therapeutic agents.

Uncovering the Bioactivity of Aurantiochytrium sp.: a Comparison of Extraction Methodologies.

Aurantiochytrium sp. is an emerging alternative source of polyunsaturated fatty acids (PUFAs), docosahexaenoic acid (DHA), and squalene, playing an important role in the phasing out of traditional fish sources for these compounds. Novel lipid extraction techniques with a focus on sustainability and low environmental footprint are being developed for this organism, but the exploration of other added-value compounds within it is still very limited. In this work, a combination of novel green extraction techniques (high hydrostatic pressure extraction (HPE) and supercritical fluid extraction (SFE)) and traditional techniques (organic solvent Soxhlet extraction and hydrodistillation (HD)) was used to obtain lipophilic extracts of Aurantiochytrium sp., which were then screened for antioxidant (DPPH radical reduction capacity and ferric-reducing antioxidant potential (FRAP) assays), lipid oxidation protection, antimicrobial, anti-aging enzyme inhibition (collagenase, elastase and hyaluronidase), and anti-inflammatory (inhibition of NO production) activities. The screening revealed promising extracts in nearly all categories of biological activity tested, with only the enzymatic inhibition being low in all extracts. Powerful lipid oxidation protection and anti-inflammatory activity were observed in most SFE samples. Ethanolic HPEs inhibited both lipid oxidation reactions and microbial growth. The HD extract demonstrated high antioxidant, antimicrobial, and anti-inflammatory activities making, it a major contender for further studies aiming at the valorization of Aurantiochytrium sp. Taken together, this study presents compelling evidence of the bioactive potential of Aurantiochytrium sp. and encourages further exploration of its composition and application.

HPLC profiling and studies on Copaifera salikounda methanol leaf extract on phenylhydrazine-induced hematotoxicity and oxidative stress in rats

Anemia is a clinical disorder orchestrated by factors such as drugs, including phenylhydrazine (PHZ). This study explored the protective roles of Copaifera salikounda methanol leaf extract (CSMLE) against PHZ-induced hematotoxicity and oxidative stress in rats. In vitro antioxidants studies of CSMLE using 1,1-diphenyl-2-picryl hydrazyl (DPPH) and ferric reducing antioxidant potential (FRAP) and quantification of CSMLE bioactive compounds using High-Performance-Liquid-Chromatography (HPLC) were done. In vivo , thirty rats in five groups (n = 6) were used. Anemia was induced intraperitoneally in groups 2 to 5 with 40 mg/kg body-weight/day PHZ for two days. Group 1 received normal saline, group 2 (untreated: negative-control), group 3 received 1 ml/kg-body-weight Vit B 12 syrup while groups 4 and 5 received 200 mg/kg and 400 mg/kg-body-weight CSMLE, respectively, daily for fourteen days. Result of CSMLE profiling showed luteolin (76.49 ng/Ml) and squalene (38.31 ng/Ml) as the most abundant flavonoid and terpenoid, respectively. In vitro CSMLE demonstrated dose-dependent antioxidant effects on DPPH and FRAP with IC 50 of 0.1085 mg/ml and 0.07344 mg/ml (EC 50 ), respectively. In vivo, CSMLE improved the body weight, red blood cell, hematocrit and hemoglobin concentrations, significantly (P < 0.05), reduced serum malondialdehyde and nitic oxide but increased reduced glutathione levels, superoxide dismutase and catalase activities and attenuated the levels of Interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) in dose-dependent manner in treated groups relative to the negative control. The spleen tissues histology results corroborated biochemical results. The results indicated that CSMLE possesses anti-anemic potentials and attenuate spleenotoxic effects of PHZ.

Effect of Cadmium and Copper Exposure on Growth, Physio-Chemicals and Medicinal Properties of Cajanus cajan L. (Pigeon Pea).

Soil contamination with heavy metals is an emerging concern in the modern era, affecting all forms of life. Pigeon pea is a multi-use shrub with medicinal and nutritional values. On the basis of a randomized complete design, we investigated in the current project the combined cadmium (Cd) and copper (Cu) effect on plant growth and physio-chemical/medicinal properties of pigeon pea. Three-week-old seedlings were grown in combined Cd and Cu amended soil with increasing metal concentrations (control, 20 + 30 mg/kg, 40 + 60 mg/kg, and 60 + 90 mg/kg) for three months. At high-dose metal cumulative stress (60 + 90 mg/kg), plant shoot and root growth in terms of plant height as well as fresh and dry weight were significantly inhibited in association with decreased photosynthetic attributes (chlorophyll a and b contents, net photosynthesis, transpiration rate, stomatal conductance, intercellular CO2 concentrations) and diminished nutrient contents. Cd and Cu at high amounts inflicted oxidative stresses as assessed in elevated lipid peroxidation (MDA), hydrogen peroxide (H2O2), and electrolyte leakage contents. Antioxidant enzyme activities, namely, those of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and glutathione peroxidase (GPX), were enhanced, along with proline content with increasing metal quantity. Phenolics and flavonoids exhibited a diverse response regarding metal concentration, and their biosynthesis was significantly suppressed at high Cd and Cu cumulative stress. The reduction in secondary metabolites may account for declined medicinal properties of pigeon pea as appraised in reduced antibacterial, 2, 2-diphenyl-1-picrylhydrazyl (DPPH), and ferric-reducing antioxidant potential (FRAP) activities. Our results clearly demonstrate that the exposure of pigeon pea to Cd- and Cu-contaminated soil might affect consumers due to the presence of metals and the negligible efficacy of the herbal products.

Comparative study of antioxidant and antimicrobial activity of different parts of lemongrass leaves and their application in the functional drink

PurposeThis study aims to compare the antioxidant and antimicrobial activity of different parts (tip, mid and base portion) of lemongrass leaves for application as a natural ingredient in the functional drink.Design/methodology/approachLemongrass leaf powder was prepared from different parts of leaves and evaluated for nutritional composition. Additionally, the extracts of different portions of lemongrass leaves were analyzed for total phenolics, free radical scavenging activities, ferric reducing antioxidant potential (FRAP) and antimicrobial activities for their application in food products.FindingsTip portion of lemongrass leaf anticipated significantly (p < 0.05) higher contents of ash, protein, calcium, potassium and iron i.e. 6.2 mg/100 g, 18 mg/100 g, 340 ppm, 819 ppm and 32 ppm, respectively. Maximum (p < 0.05) phenolics (14.7 mg GAE/100 g), 2,2-diphenyl-1-picryl-hydroxyl (86.3%) and FRAP (200 mmol/100 g) were observed in lemongrass leaf tip methanolic extracts. Moreover, lemongrass leaf tip hydro-methanolic extracts portrayed maximum zone of inhibition against E. coli and Staphylococcus aureus i.e. 16.7 and 18.2 mm, respectively.Practical implicationsThis study demonstrated higher antimicrobial and antioxidant activities of the tip of lemongrass leaves as compared with mid and base portions, hence suggesting its role in the improvement of physicochemical, antimicrobial and antioxidant properties of food products. Consequently, the application of lemongrass methanolic extract up to 10% remarkably enhanced the nutritional value and sensorial acceptance of the beverages.Originality/valueThe present research draws evidence from laboratory analysis of fresh lemongrass grown in Pakistan. The findings suggest that lemongrass methanolic extracts could be used as a nutritionally rich source of antioxidant activity in functional drinks.

Antioxidant and physicochemical properties of Chrysophyllum albidum fruit at different ripening stages

Chrysophyllum albidum (African Star Apple) fruit is an indigenous African fruit. The African Star Apple plant popularly referred to as the “alasa” tree grows widely in tropical Africa. Ripening influences biochemical processes and subsequently affects the nutritional and bioactive characteristics of the fruits. This study sampled Chrysophyllum albidum fruit of three maturity stages and determined the colour, proximate, total phenol and antioxidant characteristics of the fruits. Spectrophotometric methods were used in the determination of total antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2,2-azinobis,3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and Ferric reducing ability of plasma (FRAP) assay. Significant differences (p&lt;0.05) were observed in the colour of the fruit across all the ripening stages. The ripe stage of the fruit recorded the highest extent of lightness among all the other ripening stages. Both the ripe and over-ripe fruits appeared reddish in colour with the ripe fruit demonstrating a higher red intensity than the over-ripe fruit. The calculated metric chroma of the Chrysophyllum albidum fruits were in the range of 2.33-21.67 for the three ripening stages. Hue angle values recorded were in the range 46.46-92.00. Browning and colour indices for fruits of all the stages of development were of range 6.40-80.30 and -14.30-16.96, respectively. Proximate analysis of the three fruit categories showed that the unripe fruits had higher carbohydrate (69.27 %), crude protein (8.17 %), and crude fat (7.99 %) content relative to fruits which were ripe and over-ripe. The over-ripe fruit had higher crude fibre (7.36 %) and ash (3.86 %) content. Total phenolic content for the unripe fruit (20μg GAE/mg) was also higher than fruits of the other ripening stages. The antioxidant ability of the Chrysophyllum albidum fruit showed that the unripe stage recorded the highest DPPH (29.24 %) and ABTS (99.09 %) radical scavenging activity. The ferric reducing antioxidant potential of the fruit at different ripening stages was significantly different (p&lt;0.05) with the unripe stage recording the highest potential. The unripe fruits demonstrated higher proximate and antioxidant composition than the ripe and over-ripe fruits. Extracts from the unripe fruit can serve as useful nutraceuticals in functional food formulations.

Evaluation of acute oral toxicity, cytotoxicity, antidepressant and antioxidant activities of Japanese mint (Mentha arvensis L.) oil

Menthol-rich essential oil of Mentha arvensis L . (Japanese mint) have been identified as a valuable and new source of natural bioactive compounds with applications not only in industry but also in our daily life. The present study aimed to assess the cytotoxicity, acute toxicity, antidepressant and antioxidant activities of Japanese mint oil (JMO). Brine shrimp lethality assay was performed to investigate cytotoxicity of JMO. The acute toxicity was evaluated by Lorke's method. Forced swimming test (FST) was performed for determining antidepressant property. The antioxidant potentials were performed by DPPH scavenging assay, ferric reducing antioxidant potential (FRAP) and Fe 2+ ion chelating ability methods. In acute toxicity study, JMO showed toxic effects within 5 minutes and estimated LD 50 value was 2070.4 µl/kg body weight after 10 days oral treatment. The brine shrimp lethality assay exhibited concentration–dependent approach and the LC 50 value was determined to be 139.73 l/ml, with lower and upper limits of 82.2428 l/ml and 202.7082 l/ml, respectively. In the FST method, intraperitoneal injections of JMO emulsion at doses of 78l/kg, 156l/kg, and 225l/kg of body weight resulted in a significant (p<0.005) increase in struggling time and a reduction in immobility. This observation had a nearly identical effect to the reference drug. Besides, JMO showed weak antioxidant activity in DPPH assay with an IC 50 of 72.58±6.16387 μl/ml. But in other two methods, it exhibited strong antioxidant abilities whereas 1.4010 μl/ml reducing power for FRAP assay and 66.66% chelating rate for Fe2+ ion chelating ability test at 100 μl/ml concentration. The investigation clearly showed that JMO have significant antidepressant and cytotoxic properties whereas acute toxicity was moderate. Besides, JMO also possess considerable antioxidant activities.

Evaluation of the Antidiarrheal and Antioxidant Effects of Some Chewing Sticks Commonly Used for Oral Hygiene in Ghana.

Microbial etiology of diarrhea is a significant cause of death, especially in children in developing countries. The presence of microbes that are resistant to current treatment options for diarrhea suggests the need to find newer antimicrobial agents for treatment. Therefore, this study focused on investigating the antimicrobial effect of some Ghanaian chewing sticks commonly used for oral hygiene, Azadirachta indica, Garcinia afzelii, and Garcinia kola, against selected diarrhea-causing organisms. From the stem and bark of each plant, 70% methanolic extract was experimented on Salmonella and Shigella species, namely, Shigella sonnei, Shigella flexeneri, Salmonella typhinirium enterica, Salmonella typhi attenuated, and Klebsiella oxytoca for microbial susceptibility using the agar well diffusion method. Additionally, the antioxidant profile of the methanolic extracts were investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic) acid (ABTS) scavenging activities, and ferric-reducing antioxidant potential (FRAP) assays, while the total polyphenolic content was determined using the Folin–Ciocalteau reagent. G. afzelii and A. indica stem demonstrated the highest antimicrobial effect, inhibiting the growth of all test organisms. Additionally, the extracts demonstrated high antioxidant potential and were found to possess significant amounts of phenolic compounds. Therefore, methanolic extracts of G. afzelii and A. indica stem are promising candidates for the identification of safe novel compounds to mitigate diarrheal diseases.

Metabolic Characterization, Antioxidant Capacity, and In vitro Antiurolithiatic Potential of Achillea millefolium L.

<p style="text-align: justify;"><strong>Objectives: </strong>The current study investigated the antioxidant and antiurolithiatic potential of <em>A. millefolium </em>along with its metabolic characterization. <strong>Methods: </strong><em>A. millefolium </em>has been collected at vegetative (V) and mature (Inflorescence (I) and Stem and Leaves (S+L)) stages and extracted in ethanol. Antioxidant potential has been determined by total phenolic content, total flavonoid content, ferric reducing antioxidant potential, total antioxidants through phosphomolybdate assay and free radical scavenging assays (1,1-diphenyl-2-picrylhydrazyl and 2,2&rsquo;-azinobis( 3-ethylbenzothiazoline-6-sulfonic acid). <em>In vitro</em> antiurolithiatic activity is determined by turbidity changes in artificial urine, nucleation assay, and aggregation assay. Fourier transform-infrared spectroscopy and gas chromatography-mass spectrometry has been used to characterize the active metabolites of plant<em> A. millefolium.</em> <strong>Results:</strong> Maximum antioxidant activity was observed in Inflorescence with values 76.58 mg of GAE/g of extract, 18.82 mg of RE/g of extract, 199.799 mg of AAE/g of extract and 327.95 mg of AAE/g of extract for TPC, TFC, FRAP and total antioxidants through phosphomolybdate assay respectively. Maximum radical scavenging activity was also observed in inflorescence with 86.3% and 69.655 % inhibition against DPPH and ABTS free radicals at 1000&mu;g/ml concentration respectively. Maximum <em>in vitro </em>antiurolithiatic potential of plant<em> A. millefolium</em> observed in inflorescence i.e. 80 %, 41.84% and 63.41 % inhibition at 1000&mu;g/ml concentration for turbidity changes in artificial urine method, nucleation assay and aggregation assay respectively. FT- IR and GCMS of inflorescence of <em>A. millefolium</em> confirmed the presence of major functional groups and active metabolic compounds respectively. <strong>Conclusion: </strong>Inflorescence of plant <em>A. millefolium</em> is an excellent source of natural antioxidants with significant antiurolithiatic potential. <p style="text-align: justify;"><strong>Key words:</strong> <em>Achillea millefolium </em>L., Antioxidant activity, Antiurolithiatic activity, Inflorescence, Active metabolites.

Polysaccharides, Total Phenolic, and Flavonoid Content from Different Kenaf (Hibiscus cannabinus L.) Genotypes and Their Antioxidants and Antibacterial Properties.

Kenaf (Hibiscus cannabinus L.) is a valuable plant with a potential health benefit because of its extensive bioactive compounds. Leaf extracts of 33 kenaf genotypes were investigated for their polysaccharide, total phenolic, and flavonoid content. The antioxidant properties were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and ferric reducing antioxidant potential (FRAP) assays. Antimicrobial capacity was also assessed against Staphylococcus aureus and Escherichia coli using a disc diffusion assay. The polysaccharide content varied from 6.45–16.12 mg glucose per g DW. Total phenolic and flavonoid content ranged from 6.03–21.15 mg GAE/g DW and 1.55–9.24 mg RE/g DW, respectively. Similarly, varied values in the range 20.55–79.99% of inhibition by DPPH, 56.28–88.30% of inhibition by ABTS and 1.26–5.08 mmol Fe2+/g DW by FRAP assays were obtained for antioxidants of the genotype extracts. Extracts from CS4 and CS2 genotypes had the highest antioxidant activities. Kenaf leaves exhibited antibacterial activity against Staphylococcus aureus and Escherichia coli. Strong correlation was found between antioxidant activity with polysaccharide (DPPH, r = 0.893; ABTS, r = 0.819; FRAP, r = 0.864) and total phenolic content (DPPH, r = 0.850; ABTS, r = 0.959; FRAP, r = 0.953). The results suggested that the kenaf leaves could be used as a natural antioxidants and antimicrobial in food industries.

Fruit By-Product Processing and Bioactive Compounds

Total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant potential (FRAP), and diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity of avocado peel, avocado seeds, kiwi fruit, orange peel, pineapple peel, and pomegranate skin by-products during processing (i.e., dried, blanched, freezed, and fresh) were determined. It was hypothesized that fruit by-products would add a significant value to the food products. Heat treatments resulted in increasing TPC and TFC and reducing power of fruit by-products with avocado peels TFC of 136.9 and 63.1 mg/100 g of dried and blanched treatments, respectively, compared to 14.4 mg/100 g of fresh and 20.6 mg/100 g of freezed treatments. Orange peels TFC increased from 54.4 mg/100 g of fresh to 194.4 and 380.0 mg/100 g for blanched and dried treatments, respectively. Fresh treatments had the lowest DPPH (%) (IC50), indicating a significant effect of blanching and drying on fruit by-product antioxidant activity with some exceptions. IC50 increased from 20.0 of fresh to 39.8% of dried pineapple peel and from 6.5 to 15.0% for pomegranate skin of fresh and dried treatments, respectively. The use of fruit by-products regardless to its processing as supplements to flours would significantly increase flour’s bioactive availability.

Efficiency of Ultrasonic Incorporated Glutathione Treatment in Preserving the Physicochemical Quality and Preventing the Browning of Lotus Rhizome Slices

AbstractThis study investigated the efficacy of 40‐kHz (200 W) ultrasonication (US) for 2 min combined with glutathione (GSH) at 0.5% w/v (US + GSH) in preventing browning and preserving the physicochemical quality of lotus rhizome slices stored in a 4 ± 1 °C refrigerator. According to the visual appearance of the slices, US + GSH treatment more effectively inhibited the browning of the cut surface than did GSH or US treatment alone. US + GSH and GSH treatments were more effective than US and control treatments in preserving the slices’ colour attributes, such as their lightness and whiteness index values, and potentially retarded enzymatic browning reactions and increases in browning index and total colour difference values. Moreover, the application of US and US + GSH treatments noticeably preserved the texture of the slices. None of the treatments affected the total sugar content during storage. The US + GSH treatment markedly enhanced the ferric reducing antioxidant potential of the slices during storage. The slices treated with US, GSH, and US + GSH exhibited slower reductions in 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) free radical scavenging activity than did the controls. In conclusion, US + GSH treatment is a feasible approach for inhibiting browning, maintaining texture, and inducing antioxidant activity in lotus rhizome slices during storage.

Chitosan Displays a Potent Caloric Restriction Mimetic Effect in Senescent Rats.

Chitosan is a polysaccharide made up of β1,4-linked d-glucosamine (GlcN) and N-acetyl-GlcN. In this study, we evaluated the possible caloric restriction mimetic (CRM) effect of dietary chitosan on systemic redox status, inflammatory biomarkers, and lipid profile in plasma and erythrocyte samples of d-galactose-induced mimetically aged rats. We found a significant increase (p < 0.05) in the reactive oxygen species, protein carbonyl, fasting glucose, body weight, cholesterol, triglyceride, inflammatory markers-interleukin-6 and tumor necrosis factor-alpha in an accelerated senescent rat model. There was also a significant decrease (p < 0.05) in glutathione, advanced glycation end product in senescent rats. Chitosan treatment increased ferric-reducing antioxidant potential, glutathione, plasma membrane-reduced system in accelerated senescent model of rats. Our finding suggests that chitosan has properties similar to a CRM and can effectively maintain the redox homeostasis during the aging process in rat erythrocytes.

Age-dependent effect of continuous 'artificial light at night' on circadian rhythm in male rats: neuroprotective role of melatonin.

Circadian disruption due to artificial light at night (ALAN) is an alarming threat to modern society. In the present study we evaluated the protective effect of melatonin on age dependent redox insults and neurochemical deficits induced by ALAN in the brain of chronodisrupted rat model. Young (3months) and old (22months) male Wistar rats were exposed to ALAN along with melatonin supplementation (10mgKg-1, oral) for 10days. Results demonstrated significant increment in the pro-oxidant biomarkers: reactive oxygen species, lipid hydroperoxidation, protein carbonyl, nitric oxide while suppression in the total thiol, ferric reducing antioxidant potential level, superoxide dismutase and catalase activities in the brain of ALAN exposed groups with higher amplitude in aged rats. Further these oxidative modifications were protected by subsequent administration of melatonin. Mitochondrial complexes (C-I to C-IV) activity was significantly altered in young and old ALAN exposed groups with melatonin showing protective effect. Histopathological analysis show dense cytosolic staining and neuronal degeneration in cerebral cortex and different hippocampus regions with greater extent in old ALAN rats effectively moderated by melatonin supplementation. RT-PCR data analysis revealed melatonin effectively downregulated neuroinflammatory (IL-6, TNF α) and neurodegenerative marker (Ngb) while upregulating the aging (Sirt 1) gene expression in both young and old melatonin supplemented ALAN exposed groups. Our results may help in understanding the degree of ALAN induced photo-oxidative damage in neuronal redox homeostasis during aging. We also show that melatonin supplementation might provide a basis for amelioration of oxidative disturbances to improve circadian entrainment in aged populations.

Ultrasound-Assisted Extraction of Semi-Defatted Unripe Genipap (Genipa americana L.): Selective Conditions for the Recovery of Natural Colorants

Ultrasound-assisted extraction (UAE) of semi-defatted unripe genipap (SDG) using supercritical CO2 was performed to enhance the recovery of natural colorant iridoids genipin and geniposide. There are currently few natural sources of iridoids, and their application as colorants is scarce. The UAE resulted in extracts with blue and green colors using water and ethanol, respectively. The highest global yield and genipin content was recovered with water, and the geniposide was significantly recovered with ethanol. With water at 450 W, the UAE raised the maximum global yield (25.50 g/100 g raw material). At 150 W and 7 min, the maximum content of genipin (121.7 mg/g extract) and geniposide (312 mg/g extract) was recovered. The total phenolic content (TPC) and antioxidant capacity with the oxygen reactive antioxidant capacity (ORAC) assay were also high in aqueous extracts. Ethanolic extracts showed high ferric-reducing ability antioxidant potential (FRAP) values. UAE showed an efficient and fast method to obtain different extracts’ fractions from SDG, which have a wide spectrum of applications, especially as natural food colorants.

Effect of Microwave Power and Time on Total Phenolic Contents and Antioxidant Characteristics of Microwave Assisted Extracts of Watermelon Rind Powder

Watermelon is gaining importance as a functional food due to its therapeutic effect. The therapeutic effect of watermelon has been reported and has been attributed to antioxidant constitutes.The major component in watermelon rind is citrulline that has a strong antioxidant effect which protect body from free-radical damage.Objective:This study was conducted to investigate the effect of microwave powers (150 W, 300 W &amp; 450 W) and time intervals (1,3 &amp; 5 minutes) on total phenolic content (TPC) and total flavonoid content (TFC) and antioxidant characteristics i.e. DPPH and ferric reducing antioxidant potential (FRAP) of microwave assisted extracts of watermelon rind powder.Methods:The extracts collected after Microwave assisted extraction (MAE) of watermelon rind wereanalyzed for their antioxidant potential through different tests including total phenolic contents (TPC), total flavonoid content (TFC), DPPH assayand FRAP.Results:Microwave assisted extraction by using ethanol as a solvent at different microwave powers and various time intervals showed that total antioxidant potential was significantly higher at low microwave power such as TPC ranges obtained at 150W for 1, 3 &amp; 5 minutes of time intervals show ranges (159.84, 160.04 &amp; 169.71 mg GAE/100 g). While TFC ranges at 150W for time 1, 3 &amp; 5 minutes were (21.31, 24.15 &amp; 42.20 mg CEQ/100g) whereas DPPH ranges at 150W for time 1, 3 &amp; 5 minutes were (53.14, 54.87 &amp; 68.17 % ascorbic acid inhibition) and FRAP values at 150W for time 1, 3 &amp; 5 minutes were (201.71, 221.50 &amp; 326.43 mg FE/100g). While high microwave power 450W can result in disruption of some antioxidants at various time intervals.Conclusions:Watermelon rind is a rich source of many antioxidants andmicrowave assisted extraction technique should be implemented in the food and nutraceutical industries and microwave assisted extracts of watermelon rind should be utilize for the development of new functional food to combat many health related problems