Fermentation Of Rice Straw Research Articles

Increasing humification and reducing CH4 emission during rice straw fermentation: the role of Streptomyces rochei ZY-2

Increasing humification and reducing CH4 emission during rice straw fermentation: the role of Streptomyces rochei ZY-2

Investigation of the Microbial Diversity in the Oryza sativa Cultivation Environment and Artificial Transplantation of Microorganisms to Improve Sustainable Mycobiota.

Rice straw is not easy to decompose, it takes a long time to compost, and the anaerobic bacteria involved in the decomposition process produce a large amount of carbon dioxide (CO2), indicating that applications for rice straw need to be developed. Recycling rice straw in agricultural crops is an opportunity to increase the sustainability of grain production. Several studies have shown that the probiotic population gradually decreases in the soil, leading to an increased risk of plant diseases and decreased biomass yield. Because the microorganisms in the soil are related to the growth of plants, when the soil microbial community is imbalanced it seriously affects plant growth. We investigated the feasibility of using composted rice stalks to artificially cultivate microorganisms obtained from the Oryza sativa-planted environment for analyzing the mycobiota and evaluating applications for sustainable agriculture. Microbes obtained from the water-submerged part (group-A) and soil part (group-B) of O. sativa were cultured in an artificial medium, and the microbial diversity was analyzed with internal transcribed spacer sequencing. Paddy field soil was mixed with fermented paddy straw compost, and the microbes obtained from the soil used for O. sativa planting were designated as group-C. The paddy fields transplanted with artificially cultured microbes from group-A were designated as group-D and those from group-B were designated as group-E. We found that fungi and yeasts can be cultured in groups-A and -B. These microbes altered the soil mycobiota in the paddy fields after transplantation in groups-D and -E compared to groups-A and -B. Development in O. sativa post treatment with microbial transplantation was observed in the groups-D and -E compared to group-C. These results showed that artificially cultured microorganisms could be efficiently transplanted into the soil and improve the mycobiota. Phytohormones were involved in improving O. sativa growth and rice yield via the submerged part-derived microbial medium (group-D) or the soil part-derived microbial medium (group-E) treatments. Collectively, these fungi and yeasts may be applied in microbial transplantation via rice straw fermentation to repair soil mycobiota imbalances, facilitating plant growth and sustainable agriculture. These fungi and yeasts may be applied in microbial transplantation to repair soil mycobiota imbalances and sustainable agriculture.

Cleaner anaerobic fermentation and greenhouse gas reduction of crop straw.

Rice anaerobic fermentation is a significant source of greenhouse gas (GHG) emissions, and in order to efficiently utilize crop residue resources to reduce GHG emissions, rice straw anaerobic fermentation was regulated using lactic acid bacteria (LAB) inoculants (FG1 and TH14), grass medium (GM) to culture LAB, and Acremonim cellulolyticus (AC). Microbial community, GHG emission, dry matter (DM) loss, and anaerobic fermentation were analyzed using PacBio single-molecule real-time and anaerobic fermentation system. The epiphytic microbial diversity of fresh rice straw was extremely rich and contained certain nutrients and minerals. During ensiling, large amounts of GHG such as carbon dioxide are produced due to plant respiration, enzymatic hydrolysis reactions, and proliferation of aerobic bacteria, resulting in energy and DM loss. Addition of FG1, TH14, and AC alone improved anaerobic fermentation by decreasing pH and ammonia nitrogen content (P < 0.05) and increased lactic acid content (P < 0.05) when compared to the control, and GM showed the same additive effect as LAB inoculants. Microbial additives formed a co-occurrence microbial network system dominated by LAB, enhanced the biosynthesis of secondary metabolites, diversified the microbial metabolic environment and carbohydrate metabolic pathways, weakened the amino acid metabolic pathways, and made the anaerobic fermentation cleaner. This study is of great significance for the effective utilization of crop straw resources, the promotion of sustainable livestock production, and the reduction of GHG emissions.IMPORTANCETo effectively utilize crop by-product resources, we applied microbial additives to silage fermentation of fresh rice straw. Fresh rice straw is extremely rich in microbial diversity, which was significantly reduced after silage fermentation, and its nutrients were well preserved. Silage fermentation was improved by microbial additives, where the combination of cellulase and lactic acid bacteria acted as enzyme-bacteria synergists to promote lactic acid fermentation and inhibit the proliferation of harmful bacteria, such as protein degradation and gas production, thereby reducing GHG emissions and DM losses. The microbial additives accelerated the formation of a symbiotic microbial network system dominated by lactic acid bacteria, which regulated silage fermentation and improved microbial metabolic pathways for carbohydrates and amino acids, as well as biosynthesis of secondary metabolites.

Fermentation of Rice Straw and Its Hydrolysate with Sludge for Ethanol Production

Fermentation of Rice Straw and Its Hydrolysate with Sludge for Ethanol Production

A lysing polysaccharide monooxygenase from Aspergillus niger effectively facilitated rumen microbial fermentation of rice straw.

This study investigated the impact of Aspergillus niger lysing polysaccharide monooxygenase (AnLPMO) on in vitro rumen microbial fermentation of rice straw. AnLPMO was heterologously expressed in Escherichia coli. Fourier transform infrared spectrometry and X-ray photoelectron spectroscopy analyzed the surface structure of rice straw after AnLPMO treatment. Two in vitro experiments, coupled with 16S highthroughput sequencing and quantitative real-time polymerase chain reaction techniques, assessed the influence of AnLPMO on rumen microbial fermentation of rice straw. AnLPMO exhibited peak activity at 40°C and pH 6.5, with a preference for rice straw xylan hydrolysis, followed by Avicel. AnLPMO application led to the fractional removal of cellulose and hemicelluloses and a notable reduction in the levels of carbon elements and C-C groups present on the surface of rice straw. Compared to the control (no AnLPMO), supplementing AnLPMO at 1.1 to 2.0 U significantly enhanced in vitro digestibility of dry matter (IVDMD, p<0.01), total gas production (p<0.01), and concentrations of total volatile fatty acids (VFA, p<0.01), acetate (p<0.01), and ammonia-N (p<0.01). Particularly, the 1.4 U AnLPMO group showed a 14.8% increase in IVDMD. In the second experiment, compared to deactivated AnLPMO (1.4 U), supplementing bioactive AnLPMO at 1.4 U increased IVDMD (p = 0.01), total gas production (p = 0.04), and concentrations of total VFA (p<0.01), propionate (p<0.01), and ammonia-N (p<0.01), with a limited 9.6% increase in IVDMD. Supplementing AnLPMO stimulated the growth of ruminal bacterial taxa facilitating fiber degradation, including Proteobacteria, Spirochaetes, Succinivibrio, Rikenellaceae_RC9_ Gut_Group, Prevotelaceae_UCG-003, Desulfovibrio, Fibrobacter succinogenes, Ruminococcus albus, R. flavefaciens, Prevotella bryantii, P. ruminicola, and Treponema bryantii. These findings highlight AnLPMO's potential as a feed additive for improving rice straw utilization in ruminant production.

Bioethanol production from Hydrolysis and fermentation of Rice straw using a combination of microbial isolates

Bioethanol production from Hydrolysis and fermentation of Rice straw using a combination of microbial isolates

Solid state fermentation characteristic of rice straw using herbivore s cecum microbes

This study was aimed at identifying highly capable lignolytic microbes from nature for use in Solid State Fermentation (SSF) of rice straw. The SSF silage was prepared in laboratory scale, as the following treatments: uninoculated (control), Lactobacillus plantarum FCC 123 (LP), fiber-degrading fungi (Aspergillus sp.) from horse cecum (FF), and fiber-degrading bacteria (Enterococcus casseliflavus) from buffalo cecum (FB). Incubation was carried out for a month at room temperature. The observed parameters were: organic acids, water-soluble carbohydrate (WSC), microorganism and nutrient composition. Rice straw SSF that was inoculated with LP showed the highest quality of fermentation, indicated by significant highest lactic acid bacteria (LAB) population, and has the lowest of poor bacteria indicators (coliform, aerobic bacteria, and bacilli). The LP treatment also has the highest LAB content and lowest WSC. Among treatments, FB treatment seems to have given a similar result with LP followed by FF. While the chemical composition seems unaffected by treatments. Compared with the fresh material, all fermentation with and without inoculants has reduced neutral detergent fiber (NDF) and increased acid detergent fiber (ADF), but there were no differences among all treatments. Inoculation of both LP and FB could improve rice straw SSF silage quality, but this system could not improve fiber degradation as well as in liquid state fermentation (LSF). Keywords: Microorganism, Organic acid, Silage, Straw, Water soluble carbohydrate

Economics of baled fermented rice straw over conventional method of baling system

Economics of baled fermented rice straw over conventional method of baling system

Training on Making Fermented Rice Straw for Animal Feed in Nepo Village, Wonomulyo District, Polewali Mandar Regency

Training on making fermented rice straw for animal feed in Desa Nepo Kecamatan Wonomulyo Kabupaten Polewali Mandar aims to provide understanding to the community about making fermented rice straw for animal feed. The methods used include providing education regarding fermentation technology for rice straw waste, then discussions with participants regarding the problem that occur and obstacles in handling agricultural waste, and finally direct training in making fermented rice straw. The result of this community service activity is that participants know how to make fermented rice straw for animal feed. The conclusion of the community service activities in Desa Nepo is that the participants were able to learn about fermentation technology that can be used to process rice straw waste into highly nutritious animal feed.

Effect of Fermentation Time for a Combination of Rice Straw and Tithonia (Tithonia diversifolia) on the NDF, ADF, and Cellulose Content as Ruminant Feeding

This research aims to examine the effect of fermentation time for rice straw and tithonia (Tithonia diversifolia) on local microorganisms in the rumen of cows. This research was carried out using a Completely Randomized Design with four treatments and 4 replications. Treatments consisted of P0 (Combination of rice straw and tithonia without local microorganisms), P1 (Combination of rice straw and tithonia with 0 Day Incubation ), P2 (Combination of rice straw and tithonia with 7 Day Incubation ), and P3 (Combination of rice straw and tithonia with 14 Day Incubation. The variables measured are NDF, ADF, and Cellulose. The results of the analysis of variance showed that the effect of fermentation of rice straw and tithonia with local microorganisms in the rumen content had a very significant effect (P&lt;0.01) on NDF, ADF, and Cellulose. Based on this research, it shows that the combination of rice straw and tithonia can increase the content of NDF, ADF, and Cellulose.

The Effect of Feeding Odot Grass and Ecoenzyme Fermented Rice Straw on The Performance of Local Rams

This study aims to determine the effect of odot grass and rice straw fermented using ecoenzyme (Garbage Enzyme) on the performance of local male sheep. This research was carried out in the firuza enclosure from September 8 to November 8, 2020. The method used is a complete randomized design (RAL) with 5 practices and 4 repeats. The treatment consists of 100% odot grass + 0% fermented rice straw (PO), 75% odot grass + 25% fermented rice straw (P1), 50% odot grass + 50% fermented rice straw (P2), 25% odot grass + 75% fermented rice straw (P3), 0% odot grass + 100% fermented rice straw (P4). The parameters studied were weight gain, feed consumption, feed conversion and Income Over Feed Cost (IOFC). The results of the study had a significant effect (P&lt;0.01) on weight gain, feed consumption and feed conversion. Based on this study, it can be concluded that high feed consumption is not comparable to low weight gain resulting in high conversion.

Selection of Lactiplantibacillus plantarum strains as inoculant of rice straw fermentation and its fermentation characteristics

Selection of Lactiplantibacillus plantarum strains as inoculant of rice straw fermentation and its fermentation characteristics

Selection of Lactiplantibacillus plantarum strains as inoculant of rice straw fermentation and its fermentation characteristics

Selection of Lactiplantibacillus plantarum strains as inoculant of rice straw fermentation and its fermentation characteristics

Synergistic treatment of crude enzymes from Bacillus sp. strains to boost anaerobic fermentation of rice straw

Synergistic treatment of crude enzymes from Bacillus sp. strains to boost anaerobic fermentation of rice straw

Multinutrient Block Biological Test with the Addition of Green Betel Leaf Juice on Consumption, Digestibility, and Retention of N for Thin-tailed Sheep

Multinutrient block (MNB) is a supplement with a fairly high water content and included Intermediate Moisture. Thus, it needs to be added such preservatives ingredients, among others, green betel leaf. This also has antiprotozoal characters, so this green betel leaf has an impact on the fermentation process in the rumen. The purpose of this study is to examine the biological quality of MNB added green betel leaf in thin-tailed sheep in terms of consumption, feed digestibility, and nitrogen retention. This study used a randomized design group consisting of 3 treatments and 6 replications. The material used was MNB consisting of 50% molasses, 30% fermented rice straw, 6% clam shell flour, 3% salt, 4% urea, and 7% bentonite. This research involved 18 Thin-tailed Sheep with the weight of 11.94 ± 2,14. The addition of green betel leaf on MNB as much as 0% on T0, 3% on T1 and 6% on T2. Feeding process included commercial concentrate 65%, napier grass 25%, and MNB 10%. Each treatment was implemented on the 6 sheep as the test. The results revealed that the addition of green betel leaf juice in MNB had no significant effect (P>0.05) on the consumption of dry matter, consumption of agrie matter feed digestibility and N retention. Moreover, the provision of green betel leaf juice by 6% in MNB did not result in the consumption, digestibility, and the retention of N value in thin-tailed sheep. This research was conducted and obtained permission from the Faculty of Animal and Agriculture, Diponegoro University Semarang.

Enzymatic valorization of cellulosic and hemicellulosic-based biomasses via the production of antioxidant water-soluble hydrolyzate of maize stalks and the green bio-deinking of mixed office waste paper

AbstractBio-valorization of various biomasses provides a sustainable promising approach for the eco-friendly production of variable value-added products. Herein, the current study devoted to the enzymatic valorization of two widely available biomasses, namely, maize stalks and waste paper. The cellulytic and hemicellulytic-rich cocktail was produced through the fermentation of rice straw by a locally isolated fungal strain Aspergillus terreus. The potential applicability of the produced cocktail for the enzymatic hydrolysis of the polysaccharide constituents of maize stalks was evaluated under various strategies. The reported results indicated that the microwave pretreatment of the biomass yielding a water-soluble hydrolyzate rich in cellobiose and xylobiose, sustained by thin layer (TLC) and high-performance liquid chromatographic (HPLC) measurements, in addition to phenolic compounds. Moreover, the enzymatic hydrolysis of the extracted hemicellulosic fraction from maize stalks was rich in xylooligosaccharides and phenolic compounds higher than that released from the hydrolysis of commercial xylan. The estimated antioxidant activity of the resulted hydrolyzate was also monitored by the scavenging of 1,1-diphenyl-2-picrylhydrazyl free radical spectrophotometrically at 515 nm. Moreover, the potential applicability of the produced enzymatic cocktail was examined for the bio-deinking of waste paper. The physical, chemical, and surface morphological characteristics of the treated paper sample was compared to a blank one regarding the whiteness index, ash content, scanning electron microscopy (SEM), energy dispersive X-ray (EDX), and Fourier transform infrared spectroscopy (FTIR). On the base of the estimated results, the produced enzymatic cocktail possessed efficient dislodgement ability for the printed ink from the paper surface.

Assessment of Fermented Rice Straw as Nutritive Substances for Bio-based Plant Pot

This research aims to develop bio- based nurseries or plant pots that contain soil nourishment from agricultural waste, namely rice straw. Tapioca starch glue as a binder was mixed with rice straw and then pressed by a hydraulic compression machine to form the sheet. To promote soil nourishment, the straw was fermented. The fermentations with and without enzyme assistance were compared as well as the fermentation time. The tensile strength of the specimens was assessed through a tensile test, revealing a surprising increase in strength with fermentation. The key distinction between the specimens with and without enzyme assistance was the time it took to reach the maximum value. Without the presence of enzyme, the specimens reached a maximum tensile strength of 0. 67 MPa after 7 days of fermentation. For fermentation with enzyme assistance, this value increased to 0. 81 MPa but required 14 days of fermentation to reach its maximum point. Not only the fermentation duration but also the inclusion of enzymes showed a slightly impact on water absorption and water swelling. Spectrophotometry was employed to analyze the value of phosphorus ( P) and potassium ( K) , while the amount of nitrogen ( N) was determined by the Kjeldahl method. As expected, soil nourishment can be promoted by fermentation and boosted by enzymes. The sheet from straw fermented with the enzyme at 21 days gives thehighest soil nourishment, i.e., 17.90%w/w for total K2O, 0.40%w/w for total P2O5, and 1.89%w/w for total N.

Hydrogen and electricity potential generation from rice husks and persiculture biomass in Rio Grande do Sul, Brazil

The Southern region of Brazil has the highest grain and fruit production at a national level. Therefore, the biomass waste from rice and peach harvest in the Rio Grande do Sul (RS) state represents a promising source of available clean energy. This study seeks to demonstrate the average potential for biohydrogen and energy production by utilizing residues from peach cultivation rice straw and husks from 2014 to 2021. The findings reveal that an average of 2.89E+06 m³.year−1 of bioH2 theoretically obtained from the dark fermentation of rice husks and straw, with a biogas production of 1.49E+06 m³.year−1, derived from the anaerobic digestion of peach residues. After the purification process, the biogas yields an average of 4.73E+05 kg year−1 of H2, resulting in a total of up to 2.26E+08 kg year−1 when combined with the bioH2 obtained from the dark fermentation of rice residues. The results indicate an average energy of 8.50E+06 GJ year−1 potentially obtained by burning rice husks. Furthermore, for peach and rice biomass, the average energy potential is 3.37E+04 GJ year−1. Besides, considering using 2% of the bioH2 theoretically produced for green ammonia generation, the results show an amount of approximately 2.55E+04 tons. Therefore, this study is relevant since highlights the region's potential to produce clean energy from agricultural residues while presenting viable solutions to mitigate the negative environmental impacts caused by improper disposal of these materials. Moreover, these residues used for clean energy production contribute to the sustainable energy transition, reduce dependence on fossil fuels, decrease the need for nitrogenous fertilizer imports, and promote the implementation of the Circular Economy.

Budidaya Sapi Perah Melalui Aplikasi Teknologi Fermentasi Hijauan Pada Kelompok Peternak Andini Lestari

The Andini Lestari Dairy Farmers Group (ALDFG) runs traditional dairy farming. Farmer members are not able to store forage when conditions are in excess nor are they able to provide good forage substitutes during shortages. The aim of this activity is to apply feed fermentation technology in the ALDFG to increase livestock productivity through the feed. The target activity are 17 farmers. The method was socialization, counseling, making demonstration plots, and evaluation. All the results of the activities are discussed descriptively. The results of the activity showed that the demonstration plot for making fermented feed was successfully carried out with the output of fresh grass silage and fermented rice straw. The quality of livestock milks also improved after the application of fermented feed as supplementation of 40% forage in the ration. The survey results showed that 64.71% of farmers were interested, 23.53% said they were not interested, and 11.76% said they were hesitant to apply the fermented feed technology that had been given in the extension, there are 3 (17.65%) farmers who still consistently apply fermented feed. This activity concludes that the application of forage fermentation technology in ALDFG can improve the quality of milk and increase the interest 17.65% of the farmer to consistently apply the technology.

The quality of fermented rice straw with Trichoderma viride inoculum

The Rice straw has several nutritional weaknesses, namely its high silica and lignin content, and its low level of protein, minerals and vitamins, so the impact on digestibility is also low. Aim of present study was to evaluating nutritional efficacy of rice straw after fermentation with Trichoderma viride. The study was conducted by using complete random design (CRD). There were three different treatments with four replicates for each treatment. Fermented rice straws were treated with varying concentrations of Trichoderma viride inoculum as follows; 0.5% (P1), 1% (P2), and 1.5% (P3). Fermented rice straw's nutrients, including dry ingredients, organic material, crude fiber, crude protein, dry matter digestibility coefficients, and organic matter digestibility coefficients were measured in this study. P3 (1.5% of Trichoderma viride) treatment performed a proper nutrient, with 80.02% dry ingredients, 80.03% organic materials, 31.68% crude fiber, 5.72% protein, 38.46% dry matter digestibility coefficient, and 61.05% organic matter digestibility coefficient. In conclusion, using 1.5% Trichoderma viride to improve the quality of rice straw, as stimulator of fermentation process can be efficient in ruminant or non-ruminant nutrition.