Catfish (Pangasius pangasius) is one of popular freshwater fisheries commodities and its growth relatively fast. Fish growth and survival were affected by feed. The high-quality feed is expensive so it can be added with alternative feed ingredients such as Seligi leaf flour. Seligi leaf flour has 11.53% protein and 15.48% crude fibre. However, high crude fiber can be overcome by fermentation using probiotics. This study aims to determine the use of Seligi leaf flour fermentation (Phyllanthus buxifolius) in feed on fat retention and energy retention of catfish (Pangasius pangasius). This study used an experimental method with a completely randomized design (CRD) consisting of four treatments (0%, 2%, 4%, and 6%) with five replications. Data analysis used Analysis of Variance (ANOVA) and continued with Duncan’s Multiple Range Test. The results of the research that had been carried out were significant differences (P <0.05) on fat retention and energy retention of catfish. The highest fat retention and energy retention was in P2 treatment, while the lowest fat retention and energy retention was in P0 treatment.