Fermented Extract Research Articles

A combination of genome mining with OSMAC strategy facilitates the discovery of bioactive metabolites produced from termite-associated Streptomyces tanashiensis BYF-112.

Previously, eight new alkaloids were obtained from the fermentation extract of termite-associated Streptomyces tanashiensis BYF-112. However, genome analysis indicated the presence of many undiscovered secondary metabolites in S. tanashiensis BYF-112. Herein, 12 new alkaloids, tianwuine A-E (1-5), cephalandole C (6), venezuelines I-L (7-10), N-(4-methylphenyl-2-hydroxy) formamide (11) and N-(5-formyl-2-hydroxyphenyl) formamide (12), as well as three known metabolites (13-15) were discovered from BYF-112 based on a combination of genome mining and the one strain many compounds (OSMAC) strategy. Plausible biosynthetic pathways of 1-13 were proposed using bioinformatic analysis of the full genome of BYF-112. Partial metabolites were evaluated in vitro for their antibacterial, phytotoxic, and anti-inflammatory activities. Pyrroloformamide A (14) showed strong antibacterial activities against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Pseudomonas syringae pv. actinidae, Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola at a concentration of 50 μg per 6 mm disk. Simultaneously, pyrroloformamide A (14) also had a strong inhibitory effect on the radicle growth of Echinochloa crusgalli with an inhibition rate of 98.01% at a concentration of 100 μg/mL, equivalent to the positive 2,4-dichlorophenoxyacetic acid. Subsequently, the possible herbicidal mechanism of 14 was explored using molecular docking simulation. In addition, venezueline G (13) displayed a strong inhibitory effect of NO production, with an half-maximal inhibitory concentration (IC50) value of 2.3 μm, which was comparable with that of BAY 11-7082 with an IC50 value of 2.1 μm. These findings revealed a perspective for the development of novel bioactive drugs in the food, agricultural, and biomedical fields utilizing the metabolites of BYF-112. © 2025 Society of Chemical Industry.

A novel source of phytochemical and bioactive peptide from fermented mushroom trimming waste extract by Bifidobacterium longum

A novel source of phytochemical and bioactive peptide from fermented mushroom trimming waste extract by Bifidobacterium longum

Functional and bioactive properties of chitosan produced from Acheta domesticus with fermentation, enzymatic and microwave-assisted extraction

Chitin was recovered from crickets with green methods and converted into chitosan that was evaluated as functional and bioactive ingredient.

Fungal Submerged Fermentation of Coffee Silverskin: A Sustainable Source of Natural Meat Additives

The coffee industry, while processing coffee beans, generates residues like husk, pulp, and silverskin, which have been considered a promising source of bioactive metabolites. Recovering these metabolites offers a sustainable strategy to obtain natural food additives. Based on the above, this study aimed to determine the effect of the aqueous extract obtained from maceration and fungal fermented coffee silverskin (CSS) on ground pork meat’s oxidative and microbiological stability. Treatments used to recover bioactive compounds from CSS were the following: maceration extraction (ME) using 0, 1.5, and 3.0% of CSS (ME–0%, ME–1.5%, and ME–3%); fungal submerged fermentation extraction (FE) using 0, 1.5, and 3.0% of CSS (FE–0%, FE–1.5%, and FE–3%) and Pleurotus pulmonarius mycelium. Concerning metabolite content and bioactivity, results showed a decrease in the carbohydrate content of the ME and FE-obtained, as well as an increase in the phenol, flavonoid, and caffeoylquinic acid content. Also, an increase in radical cation scavenging activity, reducing power values and antibacterial activity of the extracts obtained with FE, was observed. Regarding pork meat homogenate treated with ME and FE extracts and subjected to oxidation with potassium ferrocyanide, results demonstrated that inclusion of FE-obtained extract led to decreased pH, lipid oxidation, metmyoglobin, and color changes (L*, a*, b*, C*, and h*), and microbial growth. These results demonstrate that CSS aqueous extract obtained with FE can be considered a potential additive for the meat industry with antioxidant and antibacterial activity. Nevertheless, further research is necessary to assess the sensory impact, biochemical mechanism, food safety, sustainability, and industrial feasibility of these extracts for broader applications.

Effects of Metabolites Derived from Guava (Psidium guajava L.) Leaf Extract Fermented by Limosilactobacillus fermentum on Hepatic Energy Metabolism via SIRT1-PGC1α Signaling in Diabetic Mice.

Type 2 diabetes mellitus (T2DM) is considered a serious risk to public health since its prevalence is rapidly increasing worldwide despite numerous therapeutics. Insulin resistance in T2DM contributes to chronic inflammation and other metabolic abnormalities that generate fat accumulation in the liver, eventually leading to the progression of metabolic dysfunction-associated fatty liver disease (MAFLD). Recently, the possibility that microbial-derived metabolites may alleviate MAFLD through enterohepatic circulation has emerged, but the underlying mechanism remains unclear. In this research, we utilized metabolites obtained from the fermentation of guava leaf extract, which is well-known for its antidiabetic activity, to investigate their effects and mechanisms on MAFLD. Diabetes was induced by a high-fat diet and streptozotocin injection (80 mg/kg body weight) twice in mice. Subsequently, mice whose fasting blood glucose levels were measured higher than 300 mg/dL were administered with metabolites of Limosilactobacillus fermentum (LF) (50 mg/kg/day) or guava leaf extract fermented by L. fermentum (GFL) (50 mg/kg/day) by gavage for 15 weeks. GFL supplementation mitigated hyperglycemia and hepatic insulin resistance. Moreover, GFL regulated abnormal hepatic histological changes and lipid profiles in diabetic mice. Furthermore, GFL enhanced energy metabolism by activating the sirtuin1 (SIRT1)/proliferator-activated receptor γ coactivator 1α (PGC1α)/peroxisome proliferator-activated receptor (PPAR)-α pathway in diabetic mice. Meanwhile, GFL supplementation suppressed hepatic inflammation in diabetic mice. Taken together, the current study elucidated that GFL could be a potential therapeutic to ameliorate hyperglycemia and hepatic steatosis by improving SIRT1/PGC-1α/ PPAR-α-related energy metabolism in T2DM.

Коллаген, гидролизованный из сырья маралов: технология получения и биохимический состав

Collagen has a complex beneficial effect on human health, which makes it a popular component in various therapeutic diets. Deer antlers are a promising source of collagen. It has been used in traditional Chinese medicine for more than 20 centuries as an additive that supports the musculoskeletal system. The article describes the effect of extraction technology on the amino acid and biochemical composition of collagen obtained from the Altai wapiti, or maral (Cervus Canadensis). The research featured hydrolysates obtained from ground skin and tendons of ten marals. The mass fraction of collagen was determined by the concentration of oxyproline. The yield of dry solids was calculated in line with State Standard GOST 316402012. The method of atomic adsorption spectroscopy made it possible to calculate the mass concentration of macro- and microelements. Amine nitrogen was detected by formol titration while the general amino acid composition was studied using the method of high-performance liquid chromatography. The list of amino acids included glycine (14.36 g/100g), proline (8.87 g/100g), and oxyproline (7.83 g/100 g). Their concentration depended on the production technology. The content of arginine and lysine increased 4–5 times during fermentation and 1.5–2.3 times during high-temperature extraction of oxyproline, glutamic acid, and threonine. A step-by-step hydrolysis protocol with fermentation and high-temperature extraction provided the maximal yield of dry solids and amino acids. In this study, the collagen hydrolyzed from maral skin and tendons was rich in glycine, proline, and oxyproline, which makes it a prospective additive to be used in products that lack these amino acids. The effect of hydrolyzed maral collagen on the human body needs further research

Impact of organic inputs on quality traits and nutrient content of Bottle gourd (Lagenaria siceraria L.): Heat map, PCA, correlation and path analysis

Nutrient deficiency in vegetables is a significant challenge for meeting the dietary needs of a growing population and improving the nutritional quality of bottle gourd (Lagenaria siceraria L.) through organic inputs can address this issue. An experiment was conducted at the Department of Vegetable Science, Tamil Nadu Agricultural University (2023-2024) aimed to enhance the nutritional quality of bottle gourd through organic methods. The research was conducted using a Randomized Block Design (RBD) with 11 treatment combinations and control, the study focused on improving its nutrient content, avoiding pesticide residues and supporting eco-friendly cultivation. Statistical analyses such as ANOVA, Principal Component Analysis (PCA), path analysis, phenotypic correlation and heat map analysis were used to evaluate the effects of organic inputs on the nutritional quality and market value of bottle gourd, ensuring better health benefits. Results from Season I and Season II revealed that treatment T9, which incorporated (Farmyard manure, Vermicompost, Neem cake, 3G extract, five-leaf extract, Panchagavya, Fish fermented extract, Egg fermented extract, Bacillus substilis), treatment T9 yielded the highest nutritional quality in bottle gourd, including protein (0.0275%), ascorbic acid (8.61%) and total ash (6.27%). It also had the highest macro and micronutrient levels, such as phosphorus (19.1 mg/g), potassium (1.74 mg/g), calcium (30.2 mg/g), copper (0.066 mg/g), iron (0.038 mg/g) and selenium (0.318 mg/g), showcasing its superior nutritional profile. These findings suggest that organic inputs, particularly in treatment T9, significantly enhance the nutritional quality of bottle gourd, offering a viable eco-friendly alternative to conventional cultivation methods.

THE EFFECT OF COLD BREW AND HOT BREW EXTRACTION METHODS AND DURATION OF FERMENTATION ON THE QUALITY OF ROBUSTA COFFEE WINE (Coffea canephora) TEMANGGUNG

This study examines the effect of fermentation duration and extraction methods (cold brew and hot brew) on the quality of robusta wine coffee. Wine coffee is a beverage resulting from the fermentation process of coffee extract which is providing a unique taste with a blend of coffee and alcohol flavors, along with a characteristic wine aroma. Through these two extraction methods, cold brew and hot brew offer different characteristics to the chemical parameters such as pH, reducing sugar content, alcohol content, caffeine content, and flavor. The research results indicate that cold brew yields a lighter flavor, while hot brew tends to have a stronger or more bitter taste. The duration of fermentation significantly affects the chemical parameters, with a decrease in pH and reducing sugar content as fermentation time increases. The alcohol content in cold brew reaches 19.93% on day 28, higher than hot brew which has 18.40% of alcohol. Organoleptic analysis results show that cold brew is preferred by the panelists, especially on the 28th day of fermentation.

Gamma-aminobutyric acid fermentation and its fermented extracts on α-glucosidase inhibition and anti-obesity effect.

Levilactobacillus brevis KCL010 was fermented in a simple medium containing 8% (w/v) of rice bran extract. We modified the carbon, nitrogen, and initial pH conditions using 10g/L of sucrose, 10g/L of yeast extract, and 5.0 of pH, respectively. To minimize the pH increase due to decarboxylation, we fermented 100mL of modified synthetic medium containing citrate-phosphate buffer (CPB, pH 5.0) of 25-200mM in 250mL Erlenmeyer flasks. After 72h of fermentation with 50mM CPB, the maximum GABA concentration and conversion efficiency were 3.42g/L and 22.39%. Furthermore, the potential α-glucosidase inhibitory activity, MTT assay, and oil red O staining were determined by fermented extracts of L. brevis KCL010. At the highest concentration of 500μg/mL, the α-glucosidase inhibition percentages for non-fermented rice bran (NFRB), rice bran fermented by L. brevis (RBFL), and GABA (analytical standard) extracts were 55.03%, 58.37%, and 59.48%, respectively. All extracts exceeded 80% viability, suggesting that there was no cytotoxic to 3T3-L1 adipocytes. The rice bran fermented by L. brevis (RBFL) extract shows a high inhibition of lipid accumulation by 29.33% compared to those of extracts.

Pasteurization and the Potential Anti-Obesity Function of Fermented Beverages: A Significant Increase in Nitrogen-Containing Aromatic Heterocyclic Compound Content

Obesity is a chronic disease that profoundly impacts human health, and the role of plant-based formulas (PBFs) in combating obesity has garnered significant interest. Studies have revealed that fermentation significantly enhances the taste, aroma, quality, and health benefits of PBF water extract, with pasteurization being the preferred sterilization technology. However, few studies have investigated the effects of pasteurization on the active components and potential functions of PBF water extract fermentation broth. To examine the impact of pasteurization on fermented water extract of Millettia speciosa Champ (FH08F) and its potential anti-obesity properties, the components of FH08F and thermal-pasteurized FH08F (FH08FS) were analyzed in this study. The analysis revealed a substantial rise in ester content following sterilization. This can be attributed to the acidic environment that promotes the esterification reaction during the heating phase. Network pharmacology was employed to thoroughly examine seven active components of upregulated compounds (URCs) with potential obesity targets, which constituted 92.97% of the total URC content, and four of them were nitrogen-containing aromatic heterocyclic compounds (NAHCs), which accounted for 90.33% of the total URC content. Upregulated NAHCs appear to actively contribute to efficacy against obesity. Molecular docking analyses have shown that theophylline, an NAHC, has the strongest binding affinity with the obesity-related target PTGS2 (Prostaglandin G/H synthase 2, 5FLG). These results imply that theophylline may directly activate PKA/PKG-mediated phosphorylated hormone-sensitive lipase (p-HSL), thereby promoting lipolysis through the cAMP signaling pathway and stimulating the catabolism of triglycerides (TGs) to combat obesity. In conclusion, pasteurization substantially alters the composition of FH08F, and NAHCs are likely to play a significant role in its potential anti-obesity function. These findings provide a scientific foundation for the potential therapeutic effect of FH08FS on obesity and associated metabolic diseases.

Chondroprotective Effect of Campylaephora hypnaeoides Extract in Primary Chondrocytes and Rat OA Model.

Campylaephora hypnaeoides (C. hypnaeoides) was extracted using fermented ethanol. The effect of fermented ethanol extract of C. hypnaeoides (FeCH) on chondrocyte viability was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-iphenyltetrazolium bromide assay, which showed no cytotoxicity at 2 mg/mL. FeCH pretreatment in IL-1β-stimulated chondrocytes significantly inhibited the accumulation of nitric oxide and prostaglandin E2, which was analyzed using the ELISA assay. In addition, protein expression levels of inflammatory-related factors, such as inducible nitric oxide synthase, cyclooxygenase-2, interleukin-6, tumor necrosis factor-alpha, and cartilage-degrading-related enzymes, such as matrix metalloproteinases-1, -3, and -13, and a disintegrin and metalloproteinase with thrombospondin motifs-4 and -5 were significantly decreased in IL-1β-stimulated chondrocytes pretreated with FeCH, which were analyzed using western blot analysis. In addition, as a result of analyzing the content of collagen type II (Col II) and proteoglycan through western blot analysis and alcian blue staining, FeCH pretreatment prevented the degradation of Col II and proteoglycan. It was analyzed through western blot analysis that the chondroprotective effect of FeCH may be mediated through MAPKs and NF-κB-signaling mechanisms. In an in vivo study, an osteoarthritis experimental animal model with damaged medial meniscus (DMM) was utilized and orally administered daily for 8 weeks after surgery. At the study end point, knee joints were harvested and subjected to histological analysis with safranin O staining. As a result, articular cartilage was significantly protected in the FeCH group compared to the DMM group. These results suggest FeCH as a candidate material for the development of pharmaceutical materials for the treatment or prevention of degenerative arthritis.

Integrated biotechnological process based on submerged fermentation and sonotrode extraction as a valuable strategy to obtain phenolic enriched extracts from moringa leaves

Integrated biotechnological process based on submerged fermentation and sonotrode extraction as a valuable strategy to obtain phenolic enriched extracts from moringa leaves

Toxicity of fermented ginger waste extract in controlling the common cutworm (Spodoptera litura) and beet armyworm (Spodoptera exigua) under laboratory conditions

Toxicity of fermented ginger waste extract in controlling the common cutworm (Spodoptera litura) and beet armyworm (Spodoptera exigua) under laboratory conditions

The Anti-ulcer Potential of Weissella cibaria Assisted Bio-fermented Product of Citrus limetta Waste Peel in Wistar Albino Rats.

There are patents available related to fermented food and beverages which enhance to human health. Citrus limetta (Mosambi) has a high content of flavonoids and exhibits antioxidant activity, which could stimulate the digestive system and be useful for gastroprotective activity. It supports digestion by neutralizing the acidic digestive juices and reducing gastric acidity. This study explored the potential of using waste peel extract from Citrus limetta to prevent ulcers. The study specifically sought to assess the anti-ulcer properties of fermented and non-fermented extracts and compare them. Further, the study looked at the potential benefits of treating or preventing ulcers with Citrus limetta waste peels and whether fermentation affected the efficacy of the treatment. Thirty female Wistar albino rats were equally distributed into five different groups. Group 1 received distilled water (20 ml/kg/b.w); Group 2 received indomethacin (mg/kg/b.w); Group 3 received omeprazole (20 mg/kg/b.w); Group 4 received aqueous extract of Mosambi peel (400 mg/kg/b.w) and Group 5 received fermented product of extract of Mosambi peel (400 mg/kg/b.w). Findings explored that, compared to non-fermented citrus fruit juice, biofermented exhibited less gastric volume (1.58 ± 0.10 ml vs. 1.8 ± 0.14 ml), reduced MDA levels (355.23 ± 100.70 μmol/mg protein vs. 454.49 ± 155.88 μmol/mg protein), and low ulcer index (0.49 ± 0.07 vs. 0.72 ± 0.14). The results suggest that the bio-fermented product of Citrus limetta peel has better anti-ulcer potential against peptic ulcer induced by indomethacin in Wistar albino rats compared to non-fermented.

Antiphytopathogenic Diphenyl Ethers from the Marine-derived Fungus Aspergillus sydowii

Background: Natural products from the marine-derived Aspergillus sp. have great potential in agricultural usage due to their broad biological activities. Objective: This study was designed to investigate the antiphytopathogenic compounds from marinederived fungus Aspergillus sydowii LW09. Methods: The compounds were isolated and purified by chromatography methods, and their structures were elucidated by analysis of the NMR and MS spectroscopic data as well as comparison with those of literature. All compounds were evaluated for antibacterial activities against phytopathogenic bacteria Pseudomonas syringae and Ralstonia solanacarum, along with spore germination inhibition of phytopathogenic fungi Fusarium oxysporum and Alternaria alternata. Results: Two diphenyl ethers violaceols I (1) and II (2), along with two alkaloids acremolin (3) and WIN 64821 (4) were isolated from the fermentation extracts of A. sydowii LW09. Compound 1 showed significant antibacterial activity against P. syringae and R. solanacarum with the same MIC values of 4 μg/mL, while compound 2 showed obvious antibacterial activity against P. syringae and R. solanacarum with MIC values of 2 and 1 μg/mL, respectively. Moreover, both 1 and 2 could inhibit the spore germination of F. oxysporum in the concentration range of 64–128 μg/mL. In addition, violaceol I (1) also inhibited the spore germination of A. alternata at 128 μg/mL. Conclusion: This study provided the potential antiphytopathogenic drug candidate for further studies.

Biological Activities and Phytochemical Constituents in the Stimulatory Potential of Tithonia diversifolia Fermented Extracts: A Review

The world food production must increase by 70% to feed this massive population through the use of chemical inputs in the new millennium. Crop culture is challenged by parasitic constraints, seedlings unavailability and soil fertility issues, which can severely reduce productivity. However, the repeated uses of chemical inputs negatively impact environment, plant and human health equilibrium. So, pathogen resistance, seedling quality and plant fertilization are severely concerned over the course of time. In this context, the use of a plant extract as Tithonia diversifolia, could be a good natural alternative to chemical inputs stimulants. The lack of knowledge of phytochemical constituents involving biologicals activities in liquid form of T. diversifolia extract, need to be improved to ensure their labeling. This study reviews biological activities and main phytochemical constituents in the stimulatory potential of T. diversifolia fermented liquid extracts. The stimulatory potential bio-efficacy of T. diversifolia extracts concerns the combination of biocontrol and green fertilizer effects. The green manure is conducted by watering or mulching on varied crops, such as rice, maize or plantain banana. Existing concerns are to expand the information amounts concerning the stimulating properties of the bioactive compounds contained in the fermented liquid extracts. It would be a key to fully demonstrate their effectiveness in stimulating the mechanism of plant growth and the protection against phytopathogens. However, this review updates on the challenge of the possibility to characterize the major bioactive compounds in the extracts. The other challenge of this current work is to suggest an eco-responsible and biological labeling product based on T. diversifolia fermented liquid extract.

Fermented Extract of Senduduk Leaf as Herbal Additive to Reduce Escherichia coli Population in Broiler Cecum

Escerichia coli is a type of bacteria that normally grows in the caecum of broilers and has the potential to become a pathogen that causes disease in broilers. This study aims to determine the effect of adding fermented sendunia leaf extract (EFDS) to drinking water on the population of Escherichia coli bacteria in the caecum of broilers. EFDS is obtained from the fermentation of fresh sendua leaves in a mixture of 1 kg of sendunia leaves + 1 l of EM4 + 16 l of sterile distilled water in a jerry can which is incubated for 2 weeks at room temperature. This study used a completely randomized design (CRD) with 4 treatments adding EFDS to drinking water at levels: A = 0% (control), B = 2%, C = 4%, and D = 6% with 5 replications. Lohman type broilers were kept for 5 weeks in cages measuring 60 x 100 cm containing 5 broilers for one treatment unit. The ration used contains 22% crude protein and 3000 Kcal/kg energy. The results of parameter measurements at the end of maintenance showed that the treatment with the addition of EFDS at levels of 4 and 6% showed that the diameter of the inhibition zone for Escherichia coli bacteria was classified as moderate level, namely 5.1-5.3 mm. A population decrease (P<0.05) was found at the EFDS level of 4% in drinking water with a population of 0.86 x106 CFU/ml with a decrease of 63.25% compared to the control. The addition of EFDS had no significant effect (P>0.05) on cecum pH and cecum length, but there was a tendency for pH to decrease (6.4-5.7) according to the level of EFDS addition. The addition of 4% EFDS in broiler drinking water is recommended in this study to control the Escherichia coli population in the broiler caecum.

Immunostimulatory Effects of Red Beet Ferment Extract on Cyclophosphamide-Induced Immunosuppression in Wistar Rats

Red beet extract contains a variety of bioactive compounds, including antioxidant and immune-stimulating properties. Red beets are rich in nutrients and have been reported to help mitigate cardiovascular disease, while also possessing potent antioxidant capacity. In this study, we sought to determine the immune-enhancing effects of red beet fermented extract. The immunosuppressive drug cyclophosphamide (Cy) and red beet fermented extract were orally administered to Wistar rats once daily for four weeks. Blood analysis was performed to measure the expression of immune cytokines. We also examined the immunostimulatory effects of red beet fermented extract by measuring the activity and viability of spleen cells and the expression of immune cytokines. The expression of immune cytokines in the blood and spleen cells was increased in the red beet fermented extract treatment group compared to that in the negative control group, which induced immunosuppression of cytokines. It also increased the activity of NK cells and improved the viability of spleen cells. In conclusion, red beet fermented extract increased the expression of immune cytokines and increased the activity of adipocytes and NK cells, confirming its immunostimulatory effect. Therefore, red beet fermented extract has the potential to be utilized as a nutraceutical ingredient to enhance immunity.

Modulating the anti-inflammatory and barrier protective effects on intestinal epithelial cells utilizing an aqueous Aspalathus linearis extract in vitro

BackgroundRooibos is a natural herbal plant containing numerous unique polyphenols which have been associated with certain health benefits. Several of these bioactive constituents have been linked to promoting gut health through its beneficial actions such as anti-inflammatory and anti-spasmodic characteristics. PurposeThe study aims to compare the anti-inflammatory and barrier protective effects of an unfermented and fermented rooibos aqueous extract on intestinal porcine epithelial cells (IPEC-J2). MethodsAqueous extracts of unfermented and fermented rooibos were prepared, and chemical and antioxidant analysis were performed of each extract. Intestinal porcine epithelial cells (IPEC-J2) were pre-treated with either unfermented or fermented aqueous rooibos extracts (0.1 and 0.05 mg/ml) then exposed to 10 µg/ml of Escherichia coli lipopolysaccharide (LPS). Cell viability, protein and gene expression of pro-inflammatory cytokines were investigated to determine the anti-inflammatory effect of both extracts. Barrier integrity of IPEC-J2 was investigated by measuring the Transepithelial electrical resistance (TEER) and quantifying tight junction gene expression. ResultsBoth the unfermented and fermented rooibos extracts have significant anti-inflammatory properties by reducing pro-inflammatory cytokine production. Interestingly for certain genes (IL-8, IL-6, IL-1B and CCL20) the fermented extracts, specifically at 0.1 mg/ml, exhibited greater effectiveness by decreasing the expression after LPS-induced inflammation whilst for another gene (TNF-α) the unfermented extract at both concentrations was more efficient in decreasing expression and ultimately protecting the IPEC-J2 cells from inflammation. When evaluating the barrier integrity, 0.1 mg/ml of the unfermented rooibos extract showed the most significant barrier protective effects through upregulation of tight junction proteins, ZO-1, Occludin as well as Claudin-4. ConclusionHigher concentration of the fermented rooibos extract prevented the inflammatory response significantly whilst the higher concentration of the unfermented rooibos extract enhanced tight junction expression and protected barrier integrity in LPS-induced inflamed intestinal cells. This is attributed to the differences in the polyphenol content of each extract and demonstrating the unique activity mechanisms of different rooibos extracts to reduce onset of early inflammation.

Investigating the effect of fermentation byLactobacillus reuteri on biochemical properties of Ceylon Cinnamon

Abstract Introduction Fermentation is an important biotechnological process that improves the biochemical properties of natural raw materials by transforming high molecular compounds into more beneficial low molecular compounds through microbial biotransformation. Ceylon cinnamon (Cinnamomum zeylanicum Blume), globally recognized as 'true cinnamon', contains high molecular weight, complex compounds that are responsible for its renowned antioxidant, antibacterial, and therapeutic properties, making it a vital component in medicinal applications1. Aims The present study aimed to investigate the effect of fermentation by Lactobacillus reuteri on selected phytochemical contents, antioxidant, and antibacterial properties of bark extracts of Ceylon cinnamon (Cinnamomum zeylanicum Blume) and to determine the optimum conditions for fermentation of Cinnamon bark extract by L. reuteri. Method Authenticated Ceylon cinnamon bark (CCB) was extracted into water using the decoction method. The water extract was fermented by L. reuteri under controlled conditions (pH 6, incubation at 35 ºC) and optimized using two parameters; the inoculum density (1.5×108 cfu/ml and 7.5×108 cfu/ml) and incubation time (2 and 4 days). After fermentation, total polyphenolic content (TPC), total flavonoid content (TFC), DPPH free radical scavenging activity and antibacterial activity of non-fermented extracts (NFE) and fermented extracts (FE) of CCB were evaluated. Antibacterial activity was determined using the agar well diffusion method against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Ethical approval was not required since no human or animal study was done during the study. Results The extract fermented with an inoculum density of 7.5×108 cfu/ml and incubated for 4 days showed the highest TFC (19.3 ± 0.3 mg RE/g) and antioxidant activity (IC50: 97.2 ± 0.8 µg/ml) in comparison to that of NFEs (TFC:13.3 ± 0.3 mg RE/g and DPPH activity: IC50: 233.7 ± 12.8 µg/ml). Moreover, the fermented samples showed a reduction in TPC, indicating possible biotransformation of compounds. and no significant change in the antibacterial activity in comparison to the non-fermented samples. Discussion and Conclusion This study was mainly focused on investigating the effect of fermentation of Ceylon cinnamon bark aqueous extract by Lactobacillus reuteri. The increased antioxidant activity and total flavonoid content are notable and could be attributed to chemical transformations of some complex flavanols including catechin gallate and epicatechin gallate present in aqueous cinnamon to catechin and epicatechin monomers by the action of enzymes secreted by L. reuteri during the fermentation process2. Hence, this study suggests that fermentation using L. reuteri has a considerable positive impact on certain biochemical properties of cinnamon water extract. Cinnamon bark is a complex matrix containing various phytochemicals that could interact with bacterial strains in ways that are not fully understood. These interactions could lead to unexpected effects or limitations during fermentation.