The aim of this study was to explore the feasibility of an alternative method for in-flight absolute radiometric calibration of the thermal infrared (TIR) channels of the Chinese meteorological satellites FengYun-2B (FY-2B) and FengYun-2C (FY-2C). The alternative method substituted radiosonde atmospheric profiles with those from the National Centers for Environmental Prediction (NCEP) reanalysis and the water surface brightness temperatures from TIR radiometers (CE312) with those from an automated hydrometeorological buoy (AHMB) system over Qinghai Lake (QHL), China. These data were then used to calculate the calibration coefficients and their uncertainty for the TIR channels of FY-2B and FY-2C. The at-sensor radiance (ASR) and at-sensor brightness temperature (ASBT) of the TIR channels of FY-2B and FY-2C were calculated by using 14 atmospheric profiles as measured by radiosonde over QHL in August 2003 and the corresponding NCEP reanalysis data, respectively. In addition, we conducted sensitivity tests to different atmospheric profiles of varying relative humidity and air temperatures on the ASR and ASBT of the TIR channels of FY-2B and FY-2C. Differences in gains between the regular and alternative methods are less than 0.005 mW m–2 sr−1 cm−1 DN−1. The sensitivity tests show that the ASR and ASBT are more sensitive to the relative humidity than the temperature in the atmospheric profile. Our results show that the proposed alternative method, of which the uncertainty is about 1.5 K for the TIR channels of FY-2B and FY-2C, is feasible for the TIR channels of various remote sensors. One of the major benefits of this alternative method is the potential for more frequent, reliable and inexpensive calibrations of the TIR sensors in operational conditions.