Speech recognition performance in the presence of competing speech is typically better for adults when the target and masker talkers are different sexes than when the target and masker talkers are the same sex. One explanation for this result is that the acoustic differences between male and female speech productions promote segregation of the two streams of speech, thus leading to a reduction in informational masking. In this study, an observer-based psychophysical procedure was used to compare infants’ (7–13 months) masked speech detection thresholds for spondee words produced by a male or a female talker in either a two-female-talker or a speech-shaped noise masker. Infants were assigned to a single testing condition. Maskers were presented continuously throughout testing at an overall level of 50 dB SPL, fixed throughout testing. Following training to an 80%-correct criterion, thresholds for the target word were measured adaptively using a 2-down, 1-up procedure. Infants’ thresholds in the two-female-talker masker were higher for the female compared to the male target word. In contrast, infants’ thresholds were similar for the female and male target words in the speech-shaped noise masker. These results suggest that introducing a different sex between the target and masker aids in the segregation of sounds for infants, as has previously been shown for adults. [Work supported by the NIH.]