Many serious health problems resulting in an enormous burden of morbidity and high health care costs are traceable to infections caused by the nosocomial pathogens; major victims are relatives of patients, the care givers who are doing their legal duties and at times the patients whose conditions could be complicated. The causative organisms of these infections and their sensitivity to antibiotics need to be ascertained. This study investigated the antibiotic sensitivity and plasmid profile of bacteria associated with nosocomial infections. A total of 45 samples were collected from children, male and female surgical wards in Ekiti State Teaching Hospital, Ado-Ekiti by using swab sticks and settling plates method. Isolation of bacteria, antibiotic sensitivity and plasmid profiling of the isolates were carried out using standard microbiological methods. The results showed that a total of 56 bacterial isolates were obtained from the 3 different wards, this includes Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Lactobacillus fermenti, Bacillus subtilis, Bacillus cereus and Micrococcus luteus. Staphylococcus aureus had the highest frequency of occurrence with 30(53.6%), followed by Staphylococcus epidermidis 10(17.7%), Escherichia coli 7(12.5%), Lactobacillus fermentii and Micrococcus luteus 3(5.4%) and Bacillus subtilis 2(3.6%) respectively, while Bacillus cereus 1(1.8%) had the least frequency of occurrence. The gram positive isolates were more resistant to the antibiotics compare to the gram negative isolates, Staphylococcus aureus was the most resistant of all the isolates followed by Staphylococcus epidermidis, the five representative resistant isolates harbored plasmid of different sizes. The findings of this study suggests that patients, care givers and visitors to the hospital should maintain good personal hygiene and proper sanitary measures to avoid nosocomial infections, therefore, surveillance of nosocomial pathogens is important to reduce hospital stay, cost, morbidity and mortality rate.
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