Female Sex Pheromone Research Articles

Female sex pheromone emission is affected by body condition, but not immune system function, in the orb‐web spider Argiope bruennichi

AbstractSex pheromones function as species and sex recognition signals but can be equally important for mate assessment and choice. Adaptive mate choice requires the covariation of signal characteristics with fitness‐relevant traits, which can be achieved by condition‐dependent costs of signalling. Given a physiological link between signal production and other vital processes, such as immune system function, the expression of the sexual signal may result in decreased body condition or increased pathogen susceptibility. According to honest signalling theory, only high‐quality individuals should be able to produce high‐quality sexual signals. We tested this prediction in the sexual signal of female wasp spiders, Argiope bruennichi. Female A. bruennichi produce the volatile pheromone trimethyl methylcitrate, which not only attracts males from a distance but also is the main foundation for short‐distance male mate choice. Recent experiments showed that females use pheromones prudently and tailor the amount of pheromone produced to their needs, suggesting costs of pheromone production. Here we manipulated female physiological condition using both immune challenge and feeding treatments in a fully factorial design. Fed or starved females were injured with a needle that was sterile or coated with bacterial lipopolysaccharides. We found no effect of immune system stimulation on pheromone production, but food stress significantly reduced the amount of pheromone produced. Importantly, this response was apparent in terms of both absolute pheromone amounts and body mass‐normalized relative pheromone amounts indicating that this effect was due to reduced pheromone production rather than reduced body surface or volume. Given the close association of the female pheromone and the citric acid cycle, the main catabolism pathway for energy production in the cell, we argue that the female pheromone of A. bruennichi may signal a female's general capacity of energy production according to the “shared pathway hypothesis” for signal honesty.

The Effect of Wind Speed on Male Potato Aphid, Macrosiphum euphorbiae, Responses to Primary Host Plant Volatiles and Female Sex Pheromone.

Simple SummaryWe demonstrate that male potato aphids respond more to a combination of volatiles from the host plant on which the species overwinters and the female sex pheromone than to the host plant alone. In both cases, the level of response declines as wind speed increases, but the higher attraction to the combined odour sources is maintained. These findings are discussed within the context of mate location by male aphids, which are insects that have little control over flight direction.In fall, alate males of the potato aphid, Macrosiphum euphorbiae (Thomas), migrate from their summer (secondary) host plants, such as potatoes, to primary host plants, such as roses, where they mate with wingless oviparae who produce the overwintering egg stage. Males are weak fliers and generally walk towards a pheromone source under windy conditions, so we tested the hypothesis that upwind walking behaviour in response to wind velocity would be affected by the volatile cues present. We compared male responses to the odour of a rugosa rose cutting alone and to the combination of host plant volatiles and the female sex pheromone under a range of wind speeds in a laboratory walking bioassay. The proportion of males responding decreased as the wind speed increased, but at all wind velocities, the responses to the combined odours were higher than to the host plant alone. However, at any given wind velocity, the speed at which responding aphids moved was not influenced by the odour source. These findings support the idea that host plant volatiles serve as long-distance cues for males and that the female sex pheromone is used once on the host plant.

Identification of Cuticular and Web Lipids of the Spider Argiope bruennichi

Emerging evidence shows that the cuticular and silk lipids of spiders are structurally more diverse than those of insects, although only a relatively low number of species have been investigated so far. As in insects, such lipids might play a role as signals in various contexts. The wasp spider Argiope bruennichi has probably the best investigated chemical communication system within spiders, including the known structure of the female sex pheromone. Recently we showed that kin-recognition in A. bruennichi could be mediated through the cuticular compounds consisting of hydrocarbons and, to a much larger proportion, of wax esters. By use of mass spectrometry and various derivatization methods, these were identified as esters of 2,4-dimethylalkanoic acids and 1-alkanols of varying chain lengths, such as tetradecyl 2,4-dimethylheptadecanoate. A representative enantioselective synthesis of this compound was performed which proved the identifications and allowed us to postulate that the natural enantiomer likely has the (2R,4R)-configuration. Chemical profiles of the silk and cuticular lipids of females were similar, while male cuticular profiles differed from those of females. Major components of the male cuticular lipids were tridecyl 2,4-dimethyl-C17-19 alkanoates, whereas those of females were slightly longer, comprising tridecyl 2,4-dimethyl-C19-21 alkanoates. In addition, minor female-specific 4-methylalkyl esters were detected.

The female sex pheromone (Z)-4-undecenal mediates flight attraction and courtship in Drosophila melanogaster

Specific mate communication and recognition underlies reproduction and hence speciation. Our study provides new insights in Drosophila melanogaster premating olfactory communication. Mate communication evolves during adaptation to ecological niches and makes use of social signals and habitat cues. Female-produced, species-specific volatile pheromone (Z)-4-undecenal (Z4-11Al) and male pheromone (Z)-11-octadecenyl acetate (cVA) interact with food odour in a sex-specific manner. Furthermore, Z4-11Al, which mediates upwind flight attraction in both sexes, also elicits courtship in experienced males. Two isoforms of the olfactory receptor Or69a are co-expressed in the same olfactory sensory neurons. Z4-11Al is perceived via Or69aB, while the food odorant (R)-linalool is a main ligand for the other variant, Or69aA. However, only Z4-11Al mediates courtship in experienced males, not (R)-linalool. Behavioural discrimination is reflected by calcium imaging of the antennal lobe, showing distinct glomerular activation patterns by these two compounds. Male sex pheromone cVA is known to affect male and female courtship at close range, but does not elicit upwind flight attraction as a single compound, in contrast to Z4-11Al. A blend of the food odour vinegar and cVA attracted females, while a blend of vinegar and female pheromone Z4-11Al attracted males, instead. Sex-specific upwind flight attraction to blends of food volatiles and male and female pheromone, respectively, adds a new element to Drosophila olfactory premating communication and is an unambiguous paradigm for identifying the behaviourally active components, towards a more complete concept of food-pheromone odour objects.

Toward an understanding of the chemical ecology of alternative reproductive tactics in the bulb mite (Rhizoglyphus robini)

BackgroundUnder strong sexual selection, certain species evolve distinct intrasexual, alternative reproductive tactics (ARTs). In many cases, ARTs can be viewed as environmentally-cued threshold traits, such that ARTs coexist if their relative fitness alternates over the environmental cue gradient. Surprisingly, the chemical ecology of ARTs has been underexplored in this context. To our knowledge, no prior study has directly quantified pheromone production for ARTs in a male-polymorphic species. Here, we used the bulb mite—in which males are either armed fighters that kill conspecifics, or unarmed scramblers (which have occasionally been observed to induce mating behavior in other males)—as a model system to gain insight into the role of pheromones in the evolutionary maintenance of ARTs. Given that scramblers forgo investment into weaponry, we tested whether scramblers produce higher quantities of the putative female sex-pheromone α-acaridial than fighters, which would improve the fitness of the scrambler phenotype through female mimicry by allowing avoidance of aggression from competitors. To this end, we sampled mites from a rich and a poor nutritional environment and quantified their production of α-acaridial through gas chromatography analysis.ResultsWe found a positive relationship between pheromone production and body size, but males exhibited a steeper slope in pheromone production with increasing size than females. Females exhibited a higher average pheromone production than males. We found no significant difference in slope of pheromone production over body size between fighters and scramblers. However, scramblers reached larger body sizes and higher pheromone production than fighters, providing some evidence for a potential female mimic strategy adopted by large scramblers. Pheromone production was significantly higher in mites from the rich nutritional environment than the poor environment.ConclusionFurther elucidation of pheromone functionality in bulb mites, and additional inter- and intrasexual comparisons of pheromone profiles are needed to determine if the observed intersexual and intrasexual differences in pheromone production are adaptive, if they are a by-product of allometric scaling, or diet-mediated pheromone production under weak selection. We argue chemical ecology offers a novel perspective for research on ARTs and other complex life-history traits.

Male attraction of Gymnandrosoma aurantianum (Lepidoptera: Tortricidae), from Guatemala, to its sex pheromone major component is not affected by the addition of secondary components

Abstract The macadamia nut borer, Gymnandrosoma aurantianum Lima (Lepidoptera: Tortricidae), is the main pest of Macadamia integrifolia Maiden and Betche (Proteaceae) in Central America. We identified five compounds in female sex pheromone gland extracts, (E)-8-dodecenyl acetate, tetradecyl acetate, dodecyl acetate, (E)-8-dodecenol, and (Z)-8-dodecenyl acetate, in a ratio of 100:44.9:33.4:6.5:1.2, respectively, from Guatemalan populations of this insect. All compounds except tetradecyl acetate elicited electroantennography responses in two- to five-day-old males. In field tests, traps baited with single compounds did not capture males, except for the major component (E)-8-dodecenyl acetate. Mixtures of (E)-8-dodecenyl acetate with secondary components were active in field tests, but the number of G. aurantianum males trapped did not differ between these mixtures and (E)-8-dodecenyl acetate alone. Doses of more than 1 mg (1.5 or 2 mg) of the major component did not significantly increase capture. The gradual loss of the amount of (E)-8-dodecenyl acetate in rubber septa under field conditions indicates that septa should be renewed every four weeks. In conclusion, the addition of secondary components to the major component of the sex pheromone gland contents does not improve capture of G. aurantianum males. Our results demonstrate that 1 mg of (E)-8-dodecenyl acetate could be used as an attractant for monitoring populations of this moth.

Both male- and female-produced pheromones influence Colorado potato beetle movement in the field.

Colorado potato beetle [Leptinotarsa decemlineata (Say); Coleoptera: Chrysomelidae] is a widespread key pest of potatoes and other solanaceous crops of the temperate Northern Hemisphere. Its evolution of multiple pesticide resistance is a continuing threat if chemical control is relied upon for pest management. An integrated approach including semiochemicals and other non-pesticidal tactics is essential to sustainable management. In addition to the known volatile aggregation pheromone produced by male adult beetles that is attractive to both females and males, there is a yet-unidentified female-produced sex pheromone. Using caged 'bait plants' in the field in Maryland, USA, we tested the attraction of male and female beetles to groups of male-only, female-only, larvae, and uninfested bait plants. Results of five field experiments demonstrate that the female-produced sex pheromone is attractive to male beetles, whereas the male-produced pheromone is attractive to both females and males. Male attraction increased with higher densities of females on bait plants, but female attraction did not change with female density on bait plants. This is the first study to demonstrate male attraction to the female sex pheromone in the field, and to our knowledge, the first to demonstrate a beetle species using bidirectional communication between males and females. Use of semiochemicals for monitoring and management of Colorado potato beetle should consider male-, female-, as well as plant-produced volatiles as important tools for pest manipulation in potato integrated pest management. Published 2021. This article is a U.S. Government work and is in the public domain in the USA.

Computational Interaction Analysis of Sirex noctilio Odorant-Binding Protein (SnocOBP7) Combined with Female Sex Pheromones and Symbiotic Fungal Volatiles

Sirex noctilio, a major forestry quarantine pest, has spread rapidly and caused serious harm. However, existing methods still need to be improved because its olfactory interaction mechanisms are poorly understood. In order to study the role of male-specific protein SnocOBP7 in the protein–ligand interactions, we selected it as the object of computational simulation and analysis. By docking it with 11 ligands and evaluating free binding energy decomposition, the three best binding ligands were found to be female sex pheromones ((Z)-7-heptacosene and (Z)-7-nonacosene) and symbiotic fungal volatiles ((−)-globulol). Binding mode analysis and computational alanine scanning suggested that five residues play key roles in the binding of each female sex pheromone to SnocOBP7, whereas two residues play key roles in (−)-globulol binding. Phe108 and Leu36 may be the crucial sites via which SnocOBP7 binds female sex pheromones, whereas Met40 may regulate the courtship behavior of males, and Leu61 may be related to mating and host finding. Our studies predicted the function of SnocOBP7 and found that the interaction between SnocOBP7 and pheromone is a complex process, and we successfully predicted its binding key amino-acid sites, providing a basis for the development of new prevention and control methods relying on female sex pheromones and symbiotic fungi.

An Expanded Survey of the Moth PBP/GOBP Clade in Bombyx mori: New Insight into Expression and Functional Roles.

We studied the expression profile and ontogeny (from the egg stage through the larval stages and pupal stages, to the elderly adult age) of four OBPs from the silkworm moth Bombyx mori. We first showed that male responsiveness to female sex pheromone in the silkworm moth B. mori does not depend on age variation; whereas the expression of BmorPBP1, BmorPBP2, BmorGOBP1, and BmorGOBP2 varies with age. The expression profile analysis revealed that the studied OBPs are expressed in non-olfactory tissues at different developmental stages. In addition, we tested the effect of insecticide exposure on the expression of the four OBPs studied. Exposure to a toxic macrolide insecticide endectocide molecule (abamectin) led to the modulated expression of all four genes in different tissues. The higher expression of OBPs was detected in metabolic tissues, such as the thorax, gut, and fat body. All these data strongly suggest some alternative functions for these proteins other than olfaction. Finally, we carried out ligand docking studies and reported that PBP1 and GOBP2 have the capacity of binding vitamin K1 and multiple different vitamins.

Pheromone binding protein is involved in temporal olfactory resolution in the silkmoth

SummaryMale moths utilize spatio-temporal female sex pheromone information to orient toward conspecific females. Pheromones are distributed as discontinuous plumes owing to air turbulence; thus, efficient tracking of intermittent stimuli is expected to require a high temporal resolution. Here, using pheromone binding protein (BmPBP1)-knockout silkmoths, we showed that a loss of functional PBP lowered the temporal sensory resolution of male antennae. This altered temporal resolution resulted in significantly reduced straight walking and longer turning behavior, which respectively occurred when males detected and lost contact with pheromones, indicating that temporal resolution was also lowered at the behavioral level. BmPBP1-knockout males required significantly longer time than wild-type males in locating pheromone sources and female moths. Our results suggest that BmPBP1 plays a critical role in determining olfactory response kinetics. Accordingly, high temporal olfactory and behavioral resolutions, as shaped by PBP, are essential for tracking pheromone plumes and locating females efficiently.

Transcriptome Analysis and Characterization of Chemosensory Genes in the Forest Pest, Dioryctria abietella (Lepidoptera: Pyralidae)

In Lepidoptera, RNA sequencing has become a useful tool in identifying chemosensory genes from antennal transcriptomes, but little attention is paid to non-antennal tissues. Though the antennae are primarily responsible for olfaction, studies have found that a certain number of chemosensory genes are exclusively or highly expressed in the non-antennal tissues, such as proboscises, legs and abdomens. In this study, we report a global transcriptome of 16 tissues from Dioryctria abietella, including chemosensory and non-chemosensory tissues. Through Illumina sequencing, totally 952,658,466 clean reads were generated, summing to 142.90 gigabases of data. Based on the transcriptome, 235 chemosensory-related genes were identified, comprising 42 odorant binding proteins (OBPs), 23 chemosensory proteins (CSPs), 75 odorant receptors (ORs), 62 gustatory receptors (GRs), 30 ionotropic receptors (IRs), and 3 sensory neuron membrane proteins (SNMPs). Compared to a previous study in this species, 140 novel genes were found. A transcriptome-wide analysis combined with PCR results revealed that except for GRs, the majority of other five chemosensory gene families in Lepidoptera were expressed in the antennae, including 160 chemosensory genes in D. abietella. Using phylogenetic and expression profiling analyses, members of the six chemosensory gene repertoires were characterized, in which 11 DabiORs were candidates for detecting female sex pheromones in D. abietella, and DabiOR23 may be involved in the sensing of plant-derived phenylacetaldehyde. Intriguingly, more than half of the genes were detected in the proboscises, and one fourth of the genes were found to have the expression in the legs. Our study not only greatly extends and improves the description of chemosensory genes in D. abietella, but also identifies potential molecular targets involved in olfaction, gustation and non-chemosensory functions for control of this pest.

Micro‐climate correlations and conserved sexual dimorphism of cuticular hydrocarbons in European populations of the jewel wasp Nasonia vitripennis

1. Protection against desiccation and chemical communication are two fundamental functions of cuticular hydrocarbons (CHCs) in insects. In the parasitoid jewel wasp Nasonia vitripennis (Walker), characterised by a cosmopolitan distribution through largely different environments, CHCs function as universally recognised female sex pheromones. However, CHC uniformity as basis for sexual recognition may conflict with the desiccation protection function, expected to display considerable flexibility through adaptation to different environmental conditions.2. We compared male and female CHC profiles of N. vitripennis across a wide latitudinal gradient in Europe and correlated their CHC variation with climatic factors associated with desiccation. Additionally, we tested male mate discrimination behaviour between populations to detect potential variations in female sexual attractiveness.3. Results did not conform to the general expectation that longer, straight‐chain CHCs occur in higher proportions in warmer and drier climates. Instead, unexpected environmental correlations of intermediate chain‐length CHCs (C31) were found exclusively in females, potentially reflecting the different life histories of the sexes in N. vitripennis.4. Furthermore, we found no indication of population‐specific male mate preference, confirming the stability of female sexual attractiveness, likely conveyed through their CHC profiles. C31 mono‐ and C33 di‐methyl‐branched alkanes were consistently and most strongly associated with sexual dimorphism, suggesting their potential role in encoding the female‐specific sexual signalling function.5. Our study sheds light on how both adaptive flexibility and conserved sexual attractiveness can potentially be integrated and encoded in CHC profiles of N. vitripennis females across a wide distribution range in Europe.

Pheromone communication among sexes of the garden cross spider Araneus diadematus

Chemical communication plays a fundamental role in many aspects of an animal’s life from assessing habitat quality to finding mating partners. Behavioural observations show that chemical communication likewise plays an important role in spiders, but the contexts and the substances involved are little explored. Here, we investigate the chemical communication in the garden cross spider Araneus diadematus (Clerck, 1757) between and within the sexes. Using choice trials, we demonstrate that males are attracted to odours of adult females, but not to those of subadult females. Our data further suggest that adult females avoid odours of conspecific adult females, possibly in order to reduce reproductive competition with other females. Cuticle and silk extracts as well as headspace samples of subadult and adult virgin females were analysed via GC–MS. Available candidate compounds for the female sex pheromone were tested via electroantennography on palps (electropalpography) of adult virgin females and on females in behavioural trials. We propose sulcatone (6-methyl-5-hepten-2-one) as a candidate substance for the female volatile pheromone and several long-chained alkanes and alcohols as candidates for contact pheromones. Apart from demonstrating that attraction of males to females depends on the latter’s developmental stage, our study suggests that pheromones can also play an important role between females, an aspect that requires further attention.

Experimental evidence for female mate choice in a noctuid moth

Sexual signal evolution is shaped by whether only one or both sexes execute mate choice. When mate choice by both sexes is considered, the same signalling modality is generally inferred for males and females. In the noctuid moth Chloridea ( Heliothis ) virescens (Lepidoptera: Noctuidae), both sexes may be signallers and responders, as both emit a sex-specific pheromone. Male mate choice is based on the female sex pheromone, perceived via the antennae, and has been well documented. However, whether females choose partners and whether their choice is based on the male pheromone are unknown. Since female mate choice is expected when males vary in quality, we reared males on two different larval diets that affected their size, which correlated positively with their fitness. When given a choice, more females mated with larger than with smaller males, and these females produced more eggs and larvae. Female choice was not affected by the absolute amount or composition of the male pheromone. Moreover, we found that antennaless females mated as readily as intact females, indicating that antennal sensory input is not required for females to mate. To determine whether females make an active choice, we studied courtship behaviour in detail and observed that females determined the outcome of courtship by moving away from the male (avoidance) or by facilitating copulation with an abdominal bend (acceptance). Additionally, we discovered that tactile sensory stimuli may be involved during courtship. Because tactile interactions may mediate contact-based (chemical) communication, we also investigated putative pheromone components on moth legs, but found no differences between the sexes. Together, our study is the first comprehensive investigation of female mate choice in a heliothine moth. • In the noctuid moth Chloridea virescens , females mated more with larger males. • Larger males were higher quality mating partners than smaller males. • The quality and quantity of male hairpencil pheromone did not explain female choice. • Females showed avoidance and acceptance behaviour, and thus chose partners actively. • Male traits other than the hairpencil pheromone likely affect female choice.

Males perceive honest information from female released sex pheromone in a moth

AbstractThere is accumulating evidence that male insects advertise their quality to conspecific females through pheromones. However, most studies of female released sex pheromone assume information transfer regarding merely the species of the female and her mating status. We show that more information is conveyed through the female sex pheromone, positioning it as an honest sexual trait. We demonstrate that females in bad physical conditions (small, starved, or old) lay significantly fewer eggs than females in good conditions (large, fed, or young). The ratio of components in the sex pheromone blend in gland extracts of the female pink bollworm moths provided honest information on most of the phenotypic conditions tested, whereas the pheromone amount in the glands provided an honest signal of quality for extreme phenotypes only. Moreover, males used the information conveyed by the female pheromone to choose their mates, approaching females that signaled higher reproductive potential. In addition, when simulating the female effect, using the synthetic pheromone blend that represents higher quality females (0.6:0.4 ZZ:ZE), more males were attracted to this blend than to the blend representing the population mean (0.5:0.5 ZZ:ZE). Both, female advertisement for males and the male choosiness, suggest that pheromones have evolved as sexual traits under directional, sexual selection. We suggest that the pheromone blend may serve as a multicomponent signal whereby each component adds information concerning the current condition of the female, and all are necessary to elicit a mate searching response.

Binding specificity of OBPs in the yellow peach moth Conogethes punctiferalis (Guenée)

AbstractOdorant‐binding proteins (OBPs) are translators of the external chemical signals, which are critical for maintaining insect life. However, few OBPs were reported in the yellow peach moth (YPM), Conogethes punctiferalis (Guenée). In the current study, five OBPs (CpunOBP1, CpunOBP2, CpunOBP7, CpunPBP2 and CpunPBP4) were expressed and purified from the antennae of the YPM. The results showed that the proteins encoded by five CpunOBPs had six conserved cysteine residues, which were typical structural features of classic OBPs. Moreover, the fluorescence competitive binding assays indicated that the binding affinity of five CpunOBPs to the selected YPM female sex pheromones, host plant volatiles and Penicillium‐inoculated apple volatiles was obviously different. The binding affinities of CpunOBP1 and CpunOBP2 with β‐ionone were the strongest. CpunOBP7 could bind with 12 host plant volatiles but was unable to interact with any one of the three tested female sex pheromones. CpunPBP2 and CpunPBP4 exhibited the highest binding affinity to female sex pheromone trans‐10‐hexadecenal among 30 tested compounds. In conclusion, these results suggest the functional differentiation of the CpunOBPs in recognizing sex pheromones, host plant volatiles and fungus‐infected host plant volatiles, which will provide new insights into selecting target proteins for YPM biocontrol.

How healthy is your mate? Sex-specific consequences of parasite infections in the moth Helicoverpa armigera

Parasites can play an important role in host sexual selection. According to the ‘Hamilton & Zuk hypothesis’, females acquire benefits from choosing parasite-free or parasite-resistant males based on their secondary sexual traits (e.g. colourful plumage) as indicators of heritable parasite resistance. However, females may also gain benefits by avoiding sexually transmitted parasites or acquiring more parental care, higher quality sperm and nuptial gifts provided by uninfected males. Here we tested how the presence of the Ophryocystis elektroscirrha -like parasite (OE-like) affected longevity, reproduction and mating frequency in the moth Helicoverpa armigera . We found that OE-like infection affected the sexes differently: the life span of uninfected males was reduced when paired with infected females, while these females had higher reproductive output than when paired with infected males. These results suggest some form of terminal investment by infected males. When we assessed the effect of OE-like infections on female calling behaviour and sex pheromone signal, we found that the OE-like parasite did not affect the female's pheromone signal in quality or quantity, but it did reduce her reproductive output, suggesting that infected females reallocate resources to maintain an attractive sex pheromone signal. In mate choice experiments, when females were the choosing sex, infected females mated significantly more often with uninfected than with infected males. Since these females produced approximately 12% more offspring, and uninfected females did not prefer uninfected or infected males, these results indicate female choice for uninfected males. Unexpectedly, when we compared the calling behaviour of the two females that were caged together, we found that infected females called significantly earlier than uninfected females, while this was not the case when females were housed alone. Thus, OE-like infection affects reproductive strategies in both males and females, but in different ways, which suggests sex-specific parasite-mediated selection. • A neogregarine parasite affects H. armigera male longevity and female reproduction. • Infected females produce more offspring with uninfected than infected males. • Male choice is affected by when female calls not by her infection status. • Infected females call significantly earlier than uninfected females. • Sex-specific effects of parasite infection result in sex-specific mating strategies.

Exposure to (Z)-11-hexadecenal [(Z)-11-16:Ald] increases Brassica nigra susceptibility to subsequent herbivory

It is well established that plants emit, detect and respond to volatile organic compounds; however, knowledge on the ability of plants to detect and respond to volatiles emitted by non-plant organisms is limited. Recent studies indicated that plants detect insect-emitted volatiles that induce defence responses; however, the mechanisms underlying this detection and defence priming is unknown. Therefore, we explored if exposure to a main component of Plutella xylostella female sex pheromone namely (Z)-11-hexadecenal [(Z)-11-16:Ald] induced detectable early and late stage defence-related plant responses in Brassica nigra. Exposure to biologically relevant levels of vapourised (Z)-11-16:Ald released from a loaded septum induced a change in volatile emissions of receiver plants after herbivore attack and increased the leaf area consumed by P. xylostella larvae. Further experiments examining the effects of the (Z)-11-16:Ald on several stages of plant defence-related responses showed that exposure to 100 ppm of (Z)-11-16:Ald in liquid state induced depolarisation of the transmembrane potential (Vm), an increase in cytosolic calcium concentration [Ca2+]cyt, production of H2O2 and an increase in expression of reactive oxygen species (ROS)-mediated genes and ROS-scavenging enzyme activity. The results suggest that exposure to volatile (Z)-11-16:Ald increases the susceptibility of B. nigra to subsequent herbivory. This unexpected finding, suggest alternative ecological effects of detecting insect pheromone to those reported earlier. Experiments conducted in vitro showed that high doses of (Z)-11-16:Ald induced defence-related responses, but further experiments should assess how specific the response is to this particular aldehyde.

Sex pheromone signal and stability covary with fitness.

If sexual signals are costly, covariance between signal expression and fitness is expected. Signal–fitness covariance is important, because it can contribute to the maintenance of genetic variation in signals that are under natural or sexual selection. Chemical signals, such as female sex pheromones in moths, have traditionally been assumed to be species-recognition signals, but their relationship with fitness is unclear. Here, we test whether chemical, conspecific mate finding signals covary with fitness in the moth Heliothis subflexa. Additionally, as moth signals are synthesized de novo every night, the maintenance of the signal can be costly. Therefore, we also hypothesized that fitness covaries with signal stability (i.e. lack of temporal intra-individual variation). We measured among- and within-individual variation in pheromone characteristics as well as fecundity, fertility and lifespan in two independent groups that differed in the time in between two pheromone samples. In both groups, we found fitness to be correlated with pheromone amount, composition and stability, supporting both our hypotheses. This study is, to our knowledge, the first to report a correlation between fitness and sex pheromone composition in moths, supporting evidence of condition-dependence and highlighting how signal–fitness covariance may contribute to heritable variation in chemical signals both among and within individuals.

(Invited) Sensors for Agricultural Pests Management

Pests are undesired living organisms which cause major threat to plants, animals, humans and other organisms. It can directly feed on the crops and indirectly spread diseases and decrease the productivity of plants. Traditionally we have been using pesticides to get rid of them, but these pesticides are chemicals which tend to accumulate in the soil and water and pose detrimental effects to all the living creatures. To reduce the major loss in food production and use of chemical pesticides, we have come up with a few innovative approaches to control pests and also detect the toxic pesticides in an easy, affordable and eco-friendly way using sensors. We had developed sensors for effective use of biopesticides such as Helicoverpa armigera nuclear polyhedrosis virus (HaNPV), Spodoptera litura nuclear polyhedrosis virus (SlNPV) and Spilosoma obliqua nuclear polyhedrosis virus (SpilNPV). We had also designed a silicon dioxide-based microelectro-mechanical system (MEMS) sensors as tool for an early selective pest detection of female sex pheromones of Helicoverpa armigera and Bactrocera oleae. This robust device can also be used to selectively measure the pheromone at femtogram levels, and it is handy for early detection of pests. The device works well in both rainy and peak summer seasons. Using these systems farmers can take necessary measures in the pest attack region without using pesticides and would be able to support organic farming.