Female Serum Research Articles

Copper and Lead Ions Anodic Stripping Voltammetry Quantitative Determination in Healthy and Cancerous Female Serum Samples

Copper and Lead Ions Anodic Stripping Voltammetry Quantitative Determination in Healthy and Cancerous Female Serum Samples

Effect of immune response on TGF-B levels in aborted females with T.gondii and Cytomegalovirus

Researched was the immune response on Transforming growth factor beta levels in aborted females infected with T.gondii and Cytomegalovirus in infected females serum. The samples were collected from aborted females from 2023/10/1 to 2024/4/1 than diagnosed in the infection with T.gondii and Cytomeglovirus by detection their IgG and IgM antibodies levels in their serum. The number of females that this study examined was 150, classified into 3 groups, the number for references during study period 30 which are group number1, 60 aborted females for unknown reasons as group 2 and 60 females infected with T.gondii and Cytomegalovirus as group 3.

The concentration of anti-muller hormone in the blood serum of reindeer depends on the level of sex hormones

Reindeer husbandry is the leading branch of agriculture for the regions of the Far North. Reindeer (Rangifer tarandus) remains poorly studied from the point of view of genetics and reproduction, which hinders the intensification of development of the industry. Research is being actively conduct in the field of genetics of the productive qualities of reindeer, but the formation and functioning of its reproductive system has been little study. Hormonal regulation of the reproductive process and the formation of hormonal status at key stages of the reproductive cycle is a significant aspect of animal reproduction. Reindeer are characterized by a pronounced seasonality of reproduction, and the study of hormonal regulation of the reproductive cycle is the main aspect of the further use of assisted reproductive technologies. The work is devoted to the analysis of the level of anti-Mullerian hormone in females during the breeding season. The studies were carried out on reindeer farms of the MAU “Directorate of the Territory of Traditional Natural Resources Management of Soyots”, Buryatia, (female, n=29). The dependence of the concentration of anti-Müllerian hormone (AMH), which is a generally accepted indicator of the female ovarian reserve, on the concentration of sex hormones (progesterone, estradiol, testosterone and luteinizing hormone) in the blood serum of females was study. Hormone levels were determined by enzyme immunoassay. A higher content of sex hormones - testosterone, estradiol, progesterone and luteinizing hormone was revealed in the group of women with an AMH concentration of >1 ng/ml than in the group of women with an AMH concentration of <1 ng/ml. The significance of the differences in progesterone was p<0.05, and in estradiol – p<0.001. A low concentration of AMH (<1 ng/ml), characterizing a reduced ovarian reserve of female reindeer, corresponds to a reduced concentration of sex hormones that support reproductive function, and, conversely, a high concentration of AMH (>1 ng/ml), and, accordingly, a high ovarian reserve the reserve corresponds to an increased concentration of sex hormones - testosterone, estradiol, progesterone and luteinizing hormone.

Feeding Parents and in ovo Injection Hatching Eggs with Omega-3 Fatty Acid and Sunflower Oil and its Effect on the Physiological Traits of Japanese Quail

It was conducted at the poultry field for the period from 12/20/2021 to 2/14/2022. 540 eggs were distributed to nine experimental and injection treatments (60 eggs per treatment). The experimental treatments for the experiment included the following: T1: negative control (without adding oil). T2: positive control (adding sunflower oil) at a rate of 0.01% to the diet. T3: added 0.01 Omega-3 to the parents diet. T4: The parents were fed omega-3 and the resulting eggs were in ovo injected with omega-3. T5: The parents were fed omega-3 and the resulting eggs were in ovo injected with sunflower oil. T6: The parents were fed with sunflower oil and the resulting eggs were in ovo injected with sunflower oil. T7: The parents were fed with sunflower oil and in ovo injected with Omega-3. T8: The parents were fed free-fed and the resulting eggs in ovo injected with omega-3. T9: The parents were free-fed and the resulting eggs were in ovo injected with sunflower oil. The results of the experiment indicated a significant increase on the number of blood cells (WBC) in the blood of both males and females, with a significant decrease in the concentration of hemoglobin (HGB) compared to the negative and positive control treatments. Feeding Japanese quail parents with in ovo injection of Omega-3 hatching eggs led to a significant increase in the concentration of total protein, albumin and globulin in the blood serum of Japanese quail males. While a significant increase in the concentration of total protein was observed with a significant decrease on the concentration of glucose and cholesterol in the blood serum of Japanese quail females compared to the control treatment.

Supplementation of chickpea flour and spinach leaves powder in Nutri bars to overcome iron deficiency in young females

ABSTRACT This study evaluated the iron-rich Nutri bar’s effect on iron-deficient females. Iron-rich Nutri-bars made with spinach powder and chickpea flour. The physicochemical profile of the bars was measured. Socioeconomic status and anthropometric data were taken for the effectiveness of the research. This effectiveness trial included 20 girls ages 20 to 25. After that, biochemical tests, including hemoglobin, ferritin, and total iron-binding capacity (TIBC) were conducted. Results showed that the iron-rich Nutri-bars (T3, 6.60) had more moisture than other treatments. T3 protein and fiber composition was 16.90% and 4.30%. In T3, crude fat and ash content were the lowest. All the proximate analyses of incorporated spinach powder and chickpea flour indicated a significant correlation (P > .005). A jury gave treatment T3 the highest score in overall acceptability. Iron and zinc concentrations were 13.2 and 5.9 mg/100 g in T3. Post-assessment height, weight, and body mass index (BMI) indicated a very significant (P > .005) connection compared to pre-assessment (P > .005). Selected young females' hemoglobin, serum ferritin, and total iron-binding capacity were tested biochemically. After the intervention, the experimental group’s mean hemoglobin (HB) was 12.21 ± 1.14 g/dl (P > .005). Increasing patterns support the current study’s iron improvement. Serum ferritin exhibited similarly substantial outcomes. TIBC dropped when iron status increased. Conclusively, iron-rich nutri-bars supplemented with spinach leaf powder and chickpea flour increased iron status in young females.

Unraveling the mechanisms of Fenugreek seed for managing different gynecological disorders: steroidal saponins and isoflavones revealed as key bioactive metabolites

This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders, particularly those related to steroidal hormones and their receptors, and the potential therapeutic effects of fenugreek (Trigonella foenum-graecum L.) constituents. The STITCH 5.0 database was utilized to identify potential molecular targets, and a compound-target network was constructed. The main targets associated with gynecological disorders included estrogen receptor beta (ESR2), estrogen-related receptor gamma (GPER1), oxytocin receptor (OXTR), progesterone receptor (PGR), prolactin receptor (PRLR), and several enzymes involved in sex hormone biosynthesis. Additionally, network topological analysis revealed that specific compounds, such as quercetin, luteolin, genistein, and vitexin, had significant interactions with the identified targets. Reverse molecular docking analysis confirmed the interactions between the identified compounds and target proteins where quercetin, luteolin, genistein, 4'-methylgenistein, trigoneoside IIB, diosgenin, and vitexin possessed the highest combined docking scores, indicating their multi-target nature. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. The chemical profiling of saponin-enriched and flavonoid-enriched fractions using UPLC/MS/MS further supported the presence of these bioactive compounds. In an animal model study, the steroidal saponins-enriched fraction of fenugreek seed exhibited a significant increase in the body weight of lactating female rats and serum prolactin levels while the flavonoids-enriched fraction showed an increase in serum estradiol levels and improved the histological structure of ovaries.

Exposure estimates of parabens from personal care products compared with biomonitoring data in human hair from Northeast China

Parabens (PBs), a class of endocrine-disrupting chemicals (EDCs), are extensively used as additives in personal care products (PCPs); however, distinguishing between endogenous and exogenous contamination from PCPs in hair remains a challenge. We conducted a comprehensive analysis of the levels, distribution patterns, impact factors, and sources of PBs in 119 human hair samples collected from Changchun, northeast China. The detection rates of methylparaben (MeP), propylparaben (PrP), and ethylparaben (EtP) in hair samples were found to be 100%. The concentration of PBs in hair followed the order of MeP (57.48 ng/g) > PrP (46.40 ng/g) > EtP (6.80 ng/g). The concentration of PrP in female hair was significantly higher (65.38 ng/g) than that observed in male hair (7.82 ng/g) (p < 0.05). The levels of excretion rates of MeP (ERMeP) and excretion rates of PrP (ERPrP) in the hair-dying samples (ERMeP: 17.89 ng/day; ERPrP: 14.15 ng/day) were found to be 2.52 and 2.40 times higher, respectively, compared to the non-hair-dying samples (ERMeP: 7.09 ng/day; ERPrP: 6.05 ng/day). However, the system exposure dosage (SED) results revealed that although hair dyes exhibited higher PBs, human exposure was found to be lower than certain PCPs. The results of the correlation analysis revealed that toner, face cream, body lotion, and hair conditioner were identified as the primary sources of PBs in male hair. Furthermore, the human exposure resulting from the utilization of female hair dye and serum exhibited a positive correlation with hair ERMeP and ERPrP levels, indicating in the screening of samples, excluding hair samples using hair dye and haircare essential oil can effectively avoid the interference caused by exogenous contamination from PCPs.

Risk of endothelial dysfunction in streptozotocin-induced diabetic Sprague-Dawley rats: Nitric oxide in focus

Endothelial dysfunction in diabetes manifests in part as reduction of nitric oxide (NO) bioavailability, which leads to inadequate relaxation of the vascular smooth muscle and a disparity between the vasoconstrictive and vasorelaxant intracellular pathways which favours a rise in vasoconstriction. In this current work, we evaluated serum Nitric oxide (NO) concentration and the activity of Nitric oxide synthase (NOS) in streptozotocin-induced diabetic male and female rats' serum. Our results showed initial and final fasting blood glucose concentration 5.30±0.16mmol/l and 5.24±.015mmol/l versus 5.68±0.18mmol/l and 5.43±0.15mmol/l in male and female controls. Among the diabetic rats, the initial and final fasting blood glucose concentration was 17.50±1.91mmol/l and 15.68±2.84mmol/l versus 20.50±4.76mmol/l and 19.40±4.13mmol/l in male and female rats respectively. NO concentration was 106.12±7.23μmol/l and 131.81±12.54 μmol/l in male and female control rats compared to 52.38±3.01μmol/l and 65.29±16.19 μmol/l in male and female diabetic rats respectively. Serum activity of NOS were 133.72±10.92μIU/l and 156.06±18.22 μIU/l in control male and female rats compared to 98.01±1.48 μIU/l, 78.89±8.39μIU/l in diabetic male and female rats respectively. The difference in both NO concentration and NOS activity was significant between diabetics and non-diabetic rats as well as between male and female diabetics rats (p&lt; 0.05). The endothelial dysfunction in diabetic animals regardless of gender may be an initial pointer to upcoming pathogenesis and we recommend routine evaluation of NO concentration and NOS activities among diabetics.

Efficacy of Oral Iron Supplementation in Treating Patients with Female Pattern Hair Loss and Low Serum Ferritin: A Pilot Study

The association between iron deficiency (ID) and hair loss is controversial. Currently, there is no standard oral iron supplementation for patients with female pattern hair loss (FPHL) and ID. We investigated the efficacy of oral iron supplementation for treating FPHL patients with ID. The results of our study revealed that oral iron supplementation combined with topical 3% minoxidil was not superior to topical 3% minoxidil alone in treating FPHL and ID.

Expression pattern of HLA-G and HLA-DR molecules on lymphocytes of women and their children with septal congenital heart defects

The aim of our study was to evaluate the features of HLA-G and HLA-DR expression on lymphocytes of women and their children with congenital heart defects (CHD) under the influence of allogeneic and autologous blood sera.38 women and their children with sporadic septal congenital heart defects (main group) were examined. The comparison groups included 21 women and their children without congenital heart disease (comparison group 1), as well as 17 apparently healthy men (comparison group 2). A total of 115 individuals were examined. The cross-match studies were carried out using a CytoFlex flow cytometer (Beckman Coulter, USA). The effects of autologous and allogeneic blood sera on HLA-G and HLA-DR expression on lymphocytes were evaluated. Statistical processing of the obtained results was carried out using Statistica for WINDOWS software packages from StatSoftInc. Version 10.0 and MedCalc 17.5.3. by the rules of variation statistics.The expression of HLA-G and HLA-DR molecules on the lymphocytes did not significantly change under the influence of autologous serum from men and women of children with CHD. At the same time, in women with more than two births of apparently healthy children, autologous serum significantly suppressed expression of HLA-G and HLA-DR on their lymphocytes. In particular, a pronounced and significant suppression was noted with autologous serum for HLA-DR molecules on CD3-positive lymphocytes. One may suggest that inflammation in the mother-embryo system is limited by this mechanism. Other significant differences concerned the effect of autologous and allogeneic (maternal) sera on the expression of HLA-G and HLA-DR molecules on the children’s lymphocytes. We have shown that in the group of children with septal CHD, autologous and allogeneic sera did not suppress the expression of HLA-G and HLA-DR on lymphocytes. At the same time, in the group of apparently healthy children, autologous and allogeneic (maternal) sera suppressed the expression of HLA-G and HLA-DR on lymphocytes. Moreover, the suppressive effect upon expression of both HLA-G and HLA-DR was significantly higher in allogeneic (maternal) sera than in autologous serum (p &lt; 0.01). This effect seems to be determined by the presence of autoand alloimmune antibodies to HLA-G and HLA-DR molecules in blood serum of multiparous women.The suppressor activity of female sera against allogeneic (embryo / fetus / child) and autologous (intrinsic) HLA-G and HLA-DR antigenic molecules may determine a protective effect related to development of septal congenital heart defects in offspring.

Gender-based relationship between serum creatinine and carotid plaque stability: A cross-sectional study in China

ObjectiveCarotid plaque instability is a risk factor for ischemic stroke, and changes in serum creatinine are associated with carotid plaque. However, the relationship between serum creatinine and carotid plaque stability is not well explained. This study aimed to interpret this relationship for clinical treatment of carotid plaque. MethodsA total of 4363 subjects aged 29–86 from December 2013 to December 2018 were included in this study. The stability of carotid plaque was determined based on ultrasound echoes and divided into two groups: carotid plaque stable group and carotid plaque unstable group. The relationship between serum creatinine and carotid plaque stability was determined using curve fitting methods as well as logistic regression. ResultsAfter age stratification, there was an inverted U-shaped curve between the stability of right carotid plaque and serum creatinine in males, When serum creatinine levels were less than 84 μmol/L, the probability of carotid plaque instability gradually increased, and the carotid plaque became stable when creatinine levels were greater than 84 μmol/L. The relationship between left carotid female plaque stability and serum creatinine showed a U-shaped curve. When serum creatinine levels were less than 80 μmol/L, the carotid plaque stability stabilized, and the probability increased when creatitine levels were more than 80 μmol/L, as the carotid plaque instability rose. ConclusionThere was an inverted U-shaped relationship between the stability of carotid plaque in the right carotid artery and serum creatinine in males, and a U-shaped relationship between the stability of carotid plaque in the left carotid artery and serum creatinine in females.

Metabolomic Profiling of Female Mink Serum during Early to Mid-Pregnancy to Reveal Metabolite Changes.

Mink embryos enter a period of diapause after the embryo develops into the blastocyst, and its reactivation is mainly caused by an increase in polyamine. The specific process of embryo diapause regulation and reactivation remains largely unexamined. This study aimed to identify changes in metabolites in the early pregnancy of mink by comparing and analyzing in serum metabolites up to twenty-nine days after mating. Blood samples were taken on the first day of mating, once a week until the fifth week. Metabolomic profiles of the serum samples taken during this period were analyzed by ultra-performance liquid chromatography/mass spectrometry. Multivariate statistical analyses identified differential metabolite expression at different time points in both positive and negative ion modes. The levels of dopamine, tyramine, L-phenylalanine, L-tyrosine, tyrosine, L-kynurenine, L-lysine, L-arginine, D-ornithine, and leucine changed significantly. These metabolites may be associated with the process of embryo diapause and subsequent reactivation.

A Bio-Indicator Pilot Study Screening Selected Heavy Metals in Female Hair, Nails, and Serum from Lifestyle Cosmetic, Canned Food, and Manufactured Drink Choices.

Lifestyles, genetic predispositions, environmental factors, and geographical regions are considered key factors of heavy metals initiatives related to health issues. Heavy metals enter the body via the environment, daily lifestyle, foods, beverages, cosmetics, and other products. The accumulation of heavy metals in the human body leads to neurological issues, carcinogenesis, failure of multiple organs in the body, and a reduction in sensitivity to treatment. We screened for Cr, Al, Pb, and Cd in selected foods, beverages, and cosmetics products depending on questionnaire outcomes from female volunteers. We also screened for Cr, Al, Pb, and Cd on hair, nails, and serum samples using inductively coupled plasma mass spectrometry (ICP-MS) from the same volunteers, and we analyzed the serum cholinesterase and complete blood picture (CBC). We performed an AutoDock study on Cr, Al, Pb, and Cd as potential ligands. Our results indicate that the most elevated heavy metal in the cosmetic sample was Al. In addition, in the food and beverages samples, it was Pb and Al, respectively. The results of the questionnaire showed that 71 percent of the female volunteers used the studied cosmetics, food, and beverages, which were contaminated with Cr, Al, Pb, and Cd, reflecting the high concentration of Cr, Al, Cd, and Pb in the three different types of biological samples of sera, nails, and hair of the same females, with 29 percent of the female volunteers not using the products in the studied samples. Our results also show an elevated level of cholinesterase in the serum of group 1 that was greater than group 2, and this result was confirmed by AutoDock. Moreover, the negative variation in the CBC result was compared with the reference ranges. Future studies should concentrate on the actions of these heavy metal contaminations and their potential health consequences for various human organs individually.

Predictors of Bleeding after Percutaneous Coronary Intervention

Background: Acute coronary syndrome patient outcomes have been improved using early invasive techniques. The aim of this study was to investigate the incidence, location, and severity of bleeding in PCI-treated cases to identify patient risk profiles and increased bleeding occurrences.&#x0D; Methods: This prospective observational study evaluated percutaneous coronary angiography in 80 patients with hypertension and diabetes mellitus who planned to undergo primary or elective PCI. The cases were separated into 2 groups; those who reported bleeding (n=11) and those who did not (n=69). All patients underwent physical examination, laboratory evaluation, 12-lead electrocardiography, and PCI.&#x0D; Results: In univariate regression analysis, age (OR: 1.09, 95% CI: 1.009 – 1.192), female gender (OR: 4.32, 95% CI: 1.157 – 16.131), history of peripheral arterial disease (OR: 7.31, 95% CI: 1.585 – 33.742), and femoral site of vascular access (OR: 9.6, 95% CI: 2.263 – 40.721) were independent predictors of major bleeding after PCI. In multivariate regression analysis, age (OR: 1.12, 95% CI:1.014 – 1.269), female gender (OR: 13.75, 95% CI: 1.983 – 161.2), history of peripheral arterial disease (OR: 43.38, 95% CI: 3.754 - 1042) and femoral site of vascular access (OR: 13.29, 95% CI: 2.233 – 128.5) were independent predictors of major bleeding after PCI.&#x0D; Conclusions: Patients who reported bleeding after PCI had a significantly higher age, prevalence of female sex, serum creatinine, and transfemoral intervention before and after intervention compared to patients who did not report bleeding, while haemoglobin and transradial intervention before and after intervention were significantly lower in the bleeding cases than in the non-bleeding cases.

Effects of different stocking densities on organ development, blood biochemical indices, and antioxidative status of breeder pigeons during the rearing period

The present experiment was conducted to investigate the effect of different stocking densities on the organ development, blood biochemical indices, and antioxidative status of breeder pigeons during the rearing period. A total of 280 (halfmaleandhalffemale) 40-day-old young pigeons were allocated into 4 groups, including 3 experimental groups (in compartments of the flying room): the high stocking density (HSD) (0.308 m3/bird), standard stocking density (SD) (0.616 m3/bird), and low stocking density (LSD) (1.232 m3/bird) and a caged control (0.04125 m3/bird). The results showed that the contents of corticosterone and heat shock protein 70 in males and the corticosterone content in females were higher in the control than in the other groups. The relative weight of liver, lung, and gizzard in males of the HSD group was the highest among the 4 treatments, whereas the abdominal fat index in the control group was higher than those in the other 3 treatments. Body weight and the relative weight of liver and abdominal fat in female pigeons in HSD group increased significantly. The levels of serum urea nitrogen and uric acid in pigeons of LSD group increased significantly, while the concentration of total cholesterol and the activity of alanine aminotransferase were higher in the control group. Ion (K+, Ca2+, and Na+) concentrations in female pigeon serum were also elevated in the control. The activity of antioxidant enzymes, including the total antioxidant capacity, superoxide dismutase, and glutathione peroxidase in pigeon breast muscle and liver had different degrees of inhibition when the space room was crowded. Moreover, the level of malondialdehyde in the liver of male caged pigeons was higher than that in the other treatments. In summary, rearing in cages or at a high density caused stress responses in the breeder pigeons. The stocking density of breeder pigeons during the rearing period should be ranged from 0.616 m3/bird to 1.232 m3/bird.

Evaluation of Launaea Angustifolia Extract Treatment on Rat Blood Serum Enzymes and Histology of Liver and Kidney

Background: Launaea angustifolia (LAN) is a wild plant grown in several Arabian countries in West Asia and North Africa. Launaea is a polymorphic inter- and intraspecific genus that includes about 54 species and 10 subspecies and is classified into eight sections. This study illustrates the effects of Launaea angustifo LAN (LAn) extract on male and female body weight and blood serum enzymes alanine aminotransferase (LAT) and Alkaline phosphatase LAN, with the histology of liver and kidneys used as an animal model for humans. Methods: A quantity of 100 mg of the extract powder was dissolved in 1 L of DMSO. Experimental rats were divided into five groups with 10 in each group (A, B, C, D, E: 100, 50 and 10 mg/kg and positive control and negative control, respectively). Result: No significant differences were observed between the treated and control-group females. However, the mean male body weight showed some variation within the 5th group (E) given the negative control (DMSO) and all treated groups presented lower body weights than the normal control (C). Analysis of the enzymatic activities of (ALT) alanine aminotransferase showed that the females in treatment group A (100 mg) and B (50 mg) had higher levels of ALT than those of group D females (10 mg) at P less than 0.05 and females in group E at P less than 0.001). The histology section of the liver and kidney and the histological structure of the rat kidney also showed no differences in all groups. Launaea angustifolia extract (LAn) was found to be safe, especially in the histology of the liver and kidney and for ALT and ALP blood parameters.

Gender-Specific Differences in Serum Sphingomyelin Species in Patients with Hepatitis C Virus Infection-Sphingomyelin Species Are Related to the Model of End-Stage Liver Disease (MELD) Score in Male Patients.

Hepatitis C virus (HCV) replication depends on cellular sphingomyelin (SM), but serum SM composition in chronic HCV infection has been hardly analyzed. In this work, 18 SM species could be quantified in the serum of 178 patients with chronic HCV infection before therapy with direct-acting antivirals (DAAs) and 12 weeks later, when therapy was completed. Six SM species were higher in the serum of females than males before therapy and nine at the end of therapy; thus, sex-specific analysis was performed. Type 2 diabetes was associated with lower serum levels of SM 36:2;O2 and 38:2;O2 in men. Serum SM species did not correlate with the viral load in both sexes. Of note, three SM species were lower in males infected with HCV genotype 3 in comparison to genotype 1 infection. These SM species normalized after viral cure. SM 38:1;O2, 40:1;O2, 41:1;O2, and 42:1;O2 (and, thus, total SM levels) were higher in the serum of both sexes at the end of therapy. In males, SM 39:1;O2 was induced in addition, and higher levels of all of these SM species were already detected at 4 weeks after therapy has been started. Serum lipids are related to liver disease severity, and in females 15 serum SM species were low in patients with liver cirrhosis before initiation of and after treatment with DAAs. The serum SM species did not correlate with the model of end-stage liver disease (MELD) score in the cirrhosis and the non-cirrhosis subgroups in females. In HCV-infected male patients, nine SM species were lower in the serum of patients with cirrhosis before DAA treatment and eleven at the end of the study. Most of the SM species showed strong negative correlations with the MELD score in the male cirrhosis patients before DAA treatment and at the end of therapy. Associations of SM species with the MELD score were not detected in the non-cirrhosis male subgroup. In summary, the current analysis identified sex-specific differences in the serum levels of SM species in HCV infection, in liver cirrhosis, and during DAA therapy. Correlations of SM species with the MELD score in male but not in female patients indicate a much closer association between SM metabolism and liver function in male patients.

Female BMI and Body Weight Is Not Associated with Oocyte Yield and Maturation in hCG, Agonist or Dual Trigger Cycles: A Large Observational Study including 5000 Cycles.

Triggering final oocyte maturation is a key step of ovarian stimulation. Although previous studies demonstrated a negative association between female BMI and serum hCG levels, little evidence is available regarding the association between oocyte yield and patients' BMI. The scope of the current study was to examine whether the efficiency of the r-hCG and triptorelin to trigger final oocyte maturation may be associated with patients' BMI or weight. This is a retrospective observational study including 5190 ovarian stimulation cycles performed between January 2019 and September 2022 in the Reproductive Medicine Department of Dexeus University Hospital. Cycles were analyzed according to the type of trigger (triptorelin vs. r-hCG vs. dual). The primary outcome measures were oocyte maturation rate (MII/oocytes) and FOI (oocytes/AFC); secondary outcomes were oocyte and MII yield. Multivariable regression analysis, adjusting for confounding factors, demonstrated that BMI was not associated with oocyte maturation rate (OR: 1.00 [95%CI: 0.99; 1.01]), FOI (Beta 0.52 [95%CI: -0.49; 1.54]), number of oocytes (Beta 0.02 [95%CI: -0.08; 0.13]) or MIIs (Beta 0.01 [95%CI: -0.08; 0.10]) retrieved. Similarly, all analyses conducted considering patients' weight failed to reveal any association. Our study demonstrates that, independent of the type of trigger, patients' BMI and weight are not associated with oocyte yield, maturation, or FOI.

The Efficacy and Safety of Dr. SKS Hair Booster Serum (a Cocktail of Micronutrients and Multivitamins) in Adult Males and Females With Androgenetic Alopecia: An Open-Label, Non-randomized, Prospective Study.

Background Androgenetic alopecia (AGA) is frequently encountered in dermatological practice; however, there is a lack of approved treatment. At present, only three therapies have been approved for on-label use in androgenetic alopecia: minoxidil, finasteride, and lower-level laser therapy. Micronutrients are primary elements in the normal hair follicle cycle, and their role in androgenetic alopecia is a growing matter of research nowadays. This study aims to investigate the clinical efficacy and safety of Dr. SKS Hair Booster Serum, a cocktail of micronutrients and multivitamins (copper, niacinamide, hyaluronic acid, thiamine, riboflavin, and biotin), in male and female patients with androgenetic alopecia. Methods We did an open-label, non-randomized, multicenter, prospective study across five hair clinic chains in India (Mumbai, Hyderabad, Jabalpur, Balaghat, and Nagpur). Eligible participants were patients with a confirmed diagnosis of androgenetic alopecia based on clinical examination and trichoscopic findings, age of 18 years or older, and any gender. Each patient received Dr. SKS Hair Booster Serum, 1 ml in volume, once a month by mesotherapy or derma roller/derma pen for up to six months. All patients were subjected to a 60-second hair counttest (comb test), hair pulltest, global photographic assessment (GPA), trichoscopy assessment, patient self-assessment questionnaire, and safety assessment at baseline and six months after the treatment. Results One thousand patients (500 males and females each) with androgenetic alopecia were analyzed. There was a significant reduction in hair fall with bulb (<0.0001) and without bulb (<0.0001) six months after the treatment versus baseline. There was a significant improvement in the number of hairs removed per pull (<0.0001), global photographic assessment score (<0.0001), hair growth rate (<0.0001), follicular hair density (<0.0001), vellus hair density (<0.0001), and terminal hair density (<0.0001) six months after the treatment versus baseline. The majority of patients (95%) were satisfied with six-monthtreatment of Dr. SKS Hair Booster Serum. No major adverse events were reported during the study. Conclusion Dr. SKS Hair Booster Serum was found to be a safe and effective treatment for androgenetic alopecia, with 95% patient self-assessment score.

Influence of Seasonal and Monthly Variation on Blood Serum Total Protein in Ctenopharyngodon idella

This study is serum protein of Ctenopharyngodon idella higher in breeding period. That value in post breeding slightly lower and pre–breathing less more than post breeding season in male. In female serum protein where higher in the post breeding. In the breeding season the serum protein is slightly lower and pre breeding value of serum protein less than breeding period.&#x0D; The mean values of female were higher in the post breathing period 5.65 gm% showing seasonal variation as 4.8 to 6.5 gm%. In the breeding season the mean value were very slightly lower side as were found in post breeding season, this period the mean value were 5.50±0.07gm% with seasonal variation of 4.4 to 6.9gm%. During summer pre breeding period the mean values 4.82±0.05 gm % with there seasonal variation of 4.0 to 5.7 gm%. In Male were higher breeding period as 5.65± 0.03 gm% showing seasonal variation as 4.5 to 6.3 gm %. In post breeding season the mean value was very slightly lower side as were found in breeding season in this period the mean value were 5.62±0.06 gm% with the seasonal variation of 4.5 to6.6gm%. During summer i.e. pre breeding period the mean value were 4.62±0.05gm% with their seasonal variation of 3.2 to5.6 gm%.