Female Pairs Research Articles

Volitional tank spawning of domestic striped bass (Morone saxatilis) using human chorionic gonadotropin (hCG) and gonadotropin releasing hormone analogue (GnRHa)- induced ‘pace-setting’ females

Seventy-one tank spawning trials were conducted to evaluate the efficacy of exogenous hormone compounds and a novel “pace-set” strategy for inducing volitional tank spawning behavior in 5th generation domestic striped bass. Female fish (4.74 ± 0.73 kg; mean ± standard deviation) were treated with human chorionic gonadotropin (hCG; 29 trials), gonadotropin releasing hormone analog (GnRHa; 39 trials) or received no hormone treatment (control; 3 trials). Spawning trials were conducted using single females placed in spawning tanks with two (12 trials) or three (38 trials) males or with paired females placed in spawning tanks with three (4 trials) males. Significant differences in egg production, fry production, hatching rate, and fry/kg female body weight were generally not observed between exogenous hormone treatment groups (alpha = 0.05), with the exception of egg production differing between paired females spawning with three males (Student's t-test, p = 0.0255). However, a trend suggesting that increasing the number of males or females within the tanks improves yield of larvae (fry/kg female body weight) was observed. The untreated control females failed to spawn within 7 days. The pace-set spawning was conducted whereby one female treated with either hCG (7 trials) or GnRHa (7 trials) was placed in a spawning tank with one untreated female and multiple males. The results of these trials show for the first time that a hormone-induced female striped bass can be used to stimulate successful volitional spawning of an untreated female in the same tank with fry/kg female body weight production similar to that of hormone-treated fish. Microsatellite-based parentage of select tank spawns and four additional trials conducted with an increased number of males (19 trials total) showed that female striped bass typically spawn with at least two males; a single pair mating was only observed for one spawning trial. These data allowed for the determination of effective broodstock size (Nb) of each tank spawning trial at between 2.00 and 5.60 when considering all male contributions. The Nb generally increased as the number of males and female fish in the tank increased (from 2.53 for one female and two males to 5.52 for two females and six males). These results indicate that domestic striped bass are promiscuous and will generally reproduce in captivity using tank spawning procedures that allow for a high level of genetic diversity to be retained among the offspring. The pace-set method reduces hormone use and may be applied to commercial striped bass production as well as captive spawning of other fish species.

Dear enemy effect in the Mexican volcano mouse Neotomodon alstoni: Implications of sex in the agonistic behaviour among neighbours

Two opposite phenomena have been found in territorial animals, the “dear enemy’’ and the “nasty neighbour’’, which refer to individuals that show less aggression toward neighbours than toward strangers and vice versa. However, the need to maintain territory should differ for males and females because sexual reproduction is the result of the different adaptive strategies of the sexes. In this study, we explore territorial behaviour in the context of dear-enemy and nasty neighbour effects in the Mexican volcano mouse (Neotomodon alstoni). Patterns of spatial relationships between individuals and the degrees of agonistic behaviour among neighbours were analysed for a period of one year. Results exhibit a greater spatial proximity between male pairs during the non-reproductive period than during the reproductive period, and greater spatial proximity between pairs of females during the reproductive period than during the non-reproductive period. The analysis of agonistic behaviour showed that there is less tolerance for distant neighbours than for nearest neighbours. However, there is a greater frequency of aggression between male pairs than between females, while females appear to exhibit non-aggressive avoidance among individuals. The results support the theory that Mexican volcano mouse exhibits the "dear enemy phenomenon".

The use of inbreeding in dairy cattle breeding

In cattle breeding during breeding season, great importance is attached to the selection of male and female pairs. The purpose of the work is to assess the effect of inbreeding on the milk production of cows of different genotypes. It was found that the main number of animals in the herd was obtained using remote inbreeding, which is 83.8%. The use of related mating led to a decrease in the age of the first calving by 0.3 months, a lactation period by 0.4, and a slight increase in milk yield by 78 kg with a decrease in the mass fraction of fat by 0.01% and protein by 0.02%, relative to outbred cows. A significant change in the productive longevity of animals obtained by outbred breeding and the use of a moderate level of inbreeding (P≤0.05) was noted. The effect of blood on Holstein breed on the productive qualities of cows was also established. An increase in blood level to 75-91% led to an increase in milk yield and qualitative indicators of productivity. Thus, the average milk yield in the group increased by 566 kg or 9.4%, while increasing the mass fraction of fat by 0.04% and protein by 0.04%. The best indicators of productivity within the genotype groups were established with a remote level of inbreeding and a genotype of Holstein breed 91-97% blood. In the future, when developing a breeding plan, it is necessary to take into account the fact that the best quality indicators of productivity, namely the mass fraction of fat and the mass fraction of protein are higher in cows with a genotype from 76 to 91% and a moderate level of inbreeding - 3.97% and 3.08%.

Are the Relationships of Lean Mass and Fat Mass With Bone Microarchitecture Causal or Due to Familial Confounders? A Novel Study of Adult Female Twin Pairs.

ABSTRACTIt is not known whether the relationships of lean mass (LM) and fat mass (FM) with bone microarchitecture and geometry are causal and/or are because of confounders, including familial confounders arising from genetic and environment effects shared by relatives. We tested the hypotheses that: (i) LM is associated with cortical bone traits, (ii) FM is associated with trabecular bone traits, and (iii) these relationships of LM and FM with bone microarchitecture and geometry have a causal component. Total body composition was quantified for 98 monozygotic (MZ) and 54 dizygotic (DZ) white female twin pairs aged 31 to 77 years. Microarchitecture at the distal tibia and distal radius was quantified using HRpQCT and StrAx software. We applied the Inference about Causation through Examination of FAmiliaL CONfounding (ICE FALCON) method. Within‐individuals, distal tibia total bone area, cortical area, cortical thickness, and trabecular number were positively associated with LM (standardized regression coefficient (β) = 0.13 to 0.43; all p < 0.05); porosity of the inner transitional zone (ITZ) was negatively associated with LM (β = −0.22; p < 0.01). Trabecular number was positively associated with FM (β = 0.40; p < 0.001), and trabecular thickness was negatively associated with FM (β = −0.27; p < 0.001). For porosity of ITZ and trabecular number, the cross‐pair cross‐trait association with LM was significant before and after adjustment for the within‐individual association with LM (all ps < 0.05). For trabecular number, the cross‐pair cross‐trait association with FM was significant before and after adjustment for the within‐individual association with FM (p < 0.01). There were no significant changes in these cross‐pair cross‐trait associations after adjustment for the within‐individual association (p = 0.06 to 0.99). Similar results were found for distal radius measures. We conclude that there was no evidence that the relationships of LM and FM with bone microarchitecture and geometry are causal; they must in part due to by familial confounders affecting both bone architecture and body composition. © 2020 The Authors. JBMR Plus published by Wiley Periodicals LLC. on behalf of American Society for Bone and Mineral Research.

Finding the Right Couple Between Microfinance Loan Officers and Clients in Terms of Gender

This paper examines the impact of the gender composition of client- loan officer pairs on loan repayment in an Ecuadorian microfinance institution. Using negative binomial regressions and random effects analyses, our results reveal that the most favourable client-loan officer pairs in terms of repayments are those with female loan officers whereas those with male loan officers are the least performing ones. We also show that a male client - female loan officer and female client-female loan officer pairs perform better when the previous loan officer the client was associated with was also a woman. Our findings have implications for theory and management. They point to relational differences between male and female loan officers when interacting with microfinance clients, these differences being confirmed through an additional qualitative research on the field.

Behavioral Teleporting of Individual Ethograms onto Inanimate Robots: Experiments on Social Interactions in Live Zebrafish.

SummarySocial behavior is widespread in the animal kingdom, and it remarkably influences human personal and professional lives. However, a thorough understanding of the mechanisms underlying social behavior is elusive. Integrating the seemingly different fields of robotics and preclinical research could bring new insight on social behavior. Toward this aim, we established “behavioral teleporting” as an experimental solution to independently manipulate multiple factors underpinning social interactions. Behavioral teleporting consists of real-time transfer of the complete ethogram of a live zebrafish onto a remotely-located robotic replica. Through parallel and simultaneous behavioral teleporting, we studied the interaction between two live fish swimming in remotely-located tanks: each live fish interacted with an inanimate robot that mirrored the behavior of the other fish, and the morphology of each robot was independently tailored. Our results indicate that behavioral teleporting can preserve natural interaction between two live animals, while allowing fine control over morphological features that modulate social behavior.

The impact of vitamin D supplementation on VDR gene expression and body composition in monozygotic twins: randomized controlled trial

Vitamin D supplementation is widely used. However, there is no consensus on the use and dosage of this supplement and the existing recommendations arise from studies based on the benefits that this nutrient can facilitate in bones. In addition, individual genetics can influence the response to supplementation, therefore, research involving monozygotic twins aims to reduce these differences in phenotypic responses. The objective of this randomised controlled study is to examine the effect of vitamin D supplementation on body composition and the expression of the vitamin D receptor (VDR) mRNA. An intervention was performed through supplementation with cholecalciferol at the concentration of 2000 IU in 90 healthy adult monozygotic twins (male or female pairs) for 2 months. The findings showed that serum vitamin D concentration increased by 65% and VDR gene expression sixty times (p = 0.001). Changes in body composition parameters were observed regarding body fat and lean mass. Our results indicate that an increase in serum vitamin D concentration may have potential therapeutic implications.

Sex classification using long-range temporal dependence of resting-state functional MRI time series.

A thorough understanding of sex differences that exist in the brains of healthy individuals is crucial for the study of neurological illnesses that exhibit phenotypic differences between males and females. Here we evaluate sex differences in regional temporal dependence of resting‐state brain activity in 195 adult male–female pairs strictly matched for total grey matter volume from the Human Connectome Project. We find that males have more persistent temporal dependence in regions within temporal, parietal, and occipital cortices. Machine learning algorithms trained on regional temporal dependence measures achieve sex classification accuracies up to 81%. Regions with the strongest feature importance in the sex classification task included cerebellum, amygdala, and frontal and occipital cortices. Secondarily, we show that even after strict matching of total gray matter volume, significant volumetric sex differences persist; males have larger absolute cerebella, hippocampi, parahippocampi, thalami, caudates, and amygdalae while females have larger absolute cingulates, precunei, and frontal and parietal cortices. Sex classification based on regional volume achieves accuracies up to 85%, highlighting the importance of strict volume‐matching when studying brain‐based sex differences. Differential patterns in regional temporal dependence between the sexes identifies a potential neurobiological substrate or environmental effect underlying sex differences in functional brain activation patterns.

Does individual personality predict male mating preference for female body size in the Trinidadian guppy?

AbstractWithin populations, individual animals vary considerably in their behaviour, including mate choice and personality. There is mounting interest in the potential covariation between these two behaviours within individuals, such that personality would influence mate choice. We experimentally tested this proposition under controlled laboratory conditions using male Trinidadian guppies (Poecilia reticulata) as a model study system. We assayed repeatedly the mating preference of individual males for females based on their body size. Additionally, we assayed repeatedly two ecologically relevant personality traits in males, namely exploration of a novel environment and boldness under a simulated predation threat. Finally, we analysed whether male mating preference and personality traits were repeatable, and tested whether the personality of individual males was correlated (covaried) with their mating preference scores. Although all but one of the measures of exploration and boldness behaviour were repeatable over time, male mating preference scores were not repeatable. Measures of male exploration and boldness were not inter‐correlated among individuals, suggesting the absence of a behavioural syndrome between exploration and boldness. Unexpectedly, males did not exhibit on average a significant mating preference for larger females over smaller ones; they chose randomly between the paired stimulus females. Overall, we found no compelling evidence for a relationship between individual personality traits and mating preference in male guppies, suggesting that personality does not predict mate choice, at least in our study population and under our experimental conditions. We discuss potential factors, other than male personality and body length, that might maintain inter‐individual variation in male mating preferences in the guppy in the wild.

Monitoring glucocorticoid metabolite concentrations as a proxy of environmental stress across important life-history stages in captive African penguins

Due to considerable global decline in wildlife population numbers and species diversity, because of various anthropogenic activities, conservationists increasingly rely on captive and managed populations as important reservoirs to ensure the survival of endangered and vulnerable species. However, very few of these facilities implement robust, non-invasive monitoring techniques to confirm the effectiveness of their management practices to address animal welfare challenges. This study assessed adrenocortical activity as an indication of environmental stress by investigating the effects of both natural (climate, life-history stages) and anthropogenic (visitor presence) factors on captive-housed African penguins. Seven male–female African penguin breeding pairs were housed in a large, naturalistic outside enclosure at the National Zoological Garden (NZG), South Africa. Weekly urofaecal samples were collected from all individuals over one-year to measure urofaecal glucocorticoid metabolite (ufGCM) concentrations. General linear mixed model analysis determined that visitor presence (for males) and rainfall (for females) were the two factors which best explained the variation in ufGCM concentrations of the study population; however, none of the environmental and anthropogenic factors monitored were found to be significant. A posthoc graphical analysis showed considerable individual variation in terms of ufGCM concentrations within and between sexes when comparing life-history stages. This study confirms that non-invasive steroid monitoring can be an effective tool set for defining and assessing environmental stressors for African penguins and potentially other captive seabirds. However, conservationists and wildlife managers should also consider that individual-, sex-, and population-specific differences in the response to environmental stressors can exist. As such, a generalized management protocol for a specific species may not be sufficient and should be customized according to the specific captive population and/or individual.

Relationship-oriented values and marital and life satisfaction among Chinese couples

Objective and background: The present study tested whether the similarity levels of relationship-oriented values among Chinese couples would be higher than those among randomly matched male–female pairs. Furthermore, we examined whether couple similarity of relationship-oriented values would predict spouses’ marital satisfaction and life satisfaction above the actor and partner effects of relationship-oriented values over time and the potential marriage cohort differences. Method: Data were retrieved from the China Family Panel Studies data set in 2010–2014. Our study included a nationally representative sample of 10,860 first-married couples who provided data on relationship-oriented values, marital satisfaction, and life satisfaction. Results: The average profile similarity on relationship-oriented values of the real couples was higher than that of the randomly matched male–female pseudo couples. Actor–Partner Interdependence Model analyses showed that (1) couple similarity of relationship-oriented values was positively associated with husbands’ and wives’ life satisfaction indirectly through wives’ marital satisfaction among couples with short to medium marital duration, even after controlling for life satisfaction 4 years ago and a set of sociodemographic variables; (2) husbands’ relationship-oriented values were positively associated with couples’ life satisfaction indirectly through husbands’ marital satisfaction among couples with short to medium and long marital duration. Conclusion: With a large dyadic sample of Chinese couples, our findings expand the literature on the significant role of couple similarity of relationship-oriented values in personal and relational well-being.

Breeding patterns of female prairie voles (Microtus ochrogaster) displaying alternative reproductive tactics.

Individuals of either sex may display alternative behaviors to obtain copulations, but few studies have examined the breeding patterns of females and males in populations where individuals of both sexes exhibit alternative reproductive tactics (ARTs). In prairie voles (Microtus ochrogaster), most adults are territorial, residing at a single nest site either as male-female pairs or as solitary individuals. However, some adults adopt nonterritorial, wandering tactics. During two field seasons monitoring prairie vole populations maintained in seminatural enclosures, we found evidence that females exhibiting different ARTs bred differentially with resident and wandering males. Females residing at a nest with a male bred significantly more often with a paired resident male, primarily their social partner, and significantly less often with male wanderers compared to single resident females or wandering females. These patterns were not due to chance, because paired resident females produced offspring with paired resident males significantly more than expected based on the relative abundance of these males in the population, whereas single resident females produced offspring with male wanderers significantly more than expected based on the proportion of male wanderers in the population. We did not find any evidence that multiple paternity was greater in the litters of single resident females and wanderer females even though these females lacked a male social partner to limit mating access by multiple males. This suggests that mate guarding by a female's male social partner was not the primary determinant of multiple paternity in the litters of females exhibiting different reproductive tactics. However, male ART did affect the likelihood of multiple paternity. Females that produced offspring with single resident or wanderer males had an increased likelihood of multiple paternity relative to females producing offspring with paired resident males. The results of this study show that female and male ARTs can affect breeding patterns.

Evaluating the Potential Association between Prenatal Exposure to Testosterone and Criminal Involvement and Criminogenic Traits

Criminologists have long been interested in understanding the mechanisms that create male-female differences in criminal involvement. One possible explanation that has generated a significant amount of interest is whether testosterone—including exposure to prenatal testosterone—might be central to male-female disparities in crime. We use this possibility as a springboard to examine the potential association between prenatal testosterone and multiple criminogenic outcomes. To do so, we analyze a sample of female dizygotic twin pairs drawn from the National Longitudinal Study of Adolescent to Adult Health and employ a proxy measure of prenatal testosterone based off of the twin testosterone transfer (TTT) hypothesis. The analyses revealed that prenatal testosterone is not associated with nonviolent criminal involvement, violent criminal involvement, psychopathic personality traits, being arrested, and being incarcerated. Interestingly, there was a small association with low self-control, but the effect was in the opposite direction of expectations. We conclude by discussing the implications and limitations of our study as well as considerations for future research.

Experience of reproduction of a ship in artificial conditions fishing facilities of the Kyzylordin Region of Kazakhstan

The article presents data on the capture of pikeperch producers from natural reservoirs, the placement of males and females in pairs for spawning in cages on nests; spawning and sexually obtained products by the factory method, incubation of pike perch eggs in the grass carp incubation apparatus, and hatching of larvae. As a result of the studies, the dates of the beginning and end of spawning, the duration of incubation of eggs and hatching of pikeperch larvae were determined. The conclusion is the optimal dates for the spawning campaign of zander; optimal sizes of manufacturers. The principal opportunity of the artificial is reproduction of zander in the conditions of fish farming in the south of Kazakhstan.

Sperm Allocation Strategies Depending on Female Quality in Medaka (Oryzias latipes).

Sperm production is costly, and males are expected to strategically allocate this potentially limiting resource to maximize their fitness. Sperm allocation theory predicts that males should adjust their sperm expenditure in relation to female quality. However, the available empirical evidence is limited. In this study, we assessed whether wild male medaka (Oryzias latipes) would allocate their sperm depending on female quality under controlled conditions. Behavioral observations revealed that spawning behavior could be classified into four stages: male quivering, female quivering, sperm release, and stay. Of these behaviors, only the duration of sperm release was positively correlated with the number of sperm ejaculated in the aquarium, suggesting that males may adjust the sperm number expended for each mating by controlling the duration of sperm release. The estimated amount of sperm released per spawning was positively and significantly correlated with the body depth and weight of paired females. This result is consistent with the prediction arising from the sperm allocation hypothesis, and suggests that male medaka may allocate their sperm depending on paired female body depth and weight. However, the number of released sperm did not correlate with the number of spawned eggs, which was positively correlated with body depth and weight of females. These results imply that medaka females may have counter-strategies against sperm allocation by males, and consequently, males might not be able to adjust the number of sperm efficiently. We disscuss our results from the perspective of sexual conflict over sperm as a limited resource.

Trial marriage model-Female mate choice under male interference.

In sexually reproducing animals, the process of mate choice by females is often mixed with the process of male-male competition. Current models of female male choice focus mainly on how females identify the higher quality of males, but neglect the effect of male-male competition on the mate choice of females. Therefore, it remains controversial what is the relative importance of two processes in forming a social bond. We propose a new 'trial marriage' model for females' mate choice. The model assumes that females unconditionally accept any male they first encounter as their mating partner, and then conditionally switch mates to a new male of higher quality than their current partner when male-male competition occurs. This model was tested in the green weevil Hypomeces squamosus by exploring how females switched mates when males' mating interference was experimentally induced. The likelihood that females switched mates, as well as their conditional acceptance criteria of a new mate, was both raised with the intensity of males' mating interference that was manipulated in an enhanced encounter rate experiment, and in male introduction or stepwise removal experiments. These experimental findings confirm that a 'trial marriage' strategy occurs during females' mate choice. Compared with other strategies, it is more beneficial for females to choose a better mate without paying the costs of identifying males as suggested by the 'trial marriage' strategy. More importantly, using the current partner quality as the conditional acceptance threshold of new mates, females can choose better males in future encounters with potential mates. In the green weevils, males' preference for larger females and the higher possibility of the largest male winning an interference are mixed together when males' mating interference reaches a higher intensity. Therefore, the consequence of a male interference will determine which male could be chosen by a female. Under this condition, conditional acceptance of the winner becomes the most beneficial strategy of females in choosing their mates. We thus suggest that the 'trial marriage' strategy would be more efficient when males' mating interference becomes the determinant factor of females' mate choice.

Gut microbial pulse provides nutrition for parental provisioning in incipient termite colonies

Success in aerial dispersal involves a trade-off between propagule size and dispersal ability, such that reducing weight may increase dispersal capability. Therefore, dispersing propagules such as plant seeds, fungal spores, and insect alates need to settle in new habitats with very limited resources. In termites, the time from alate dispersal to colony foundation is the most critical period in their life cycle, because a male and female pair establishes a new colony without help from others. In addition, termites depend largely on gut microbes for their nutrition. However, little is known about how termite founding pairs with limited resources establish new colonies and raise their first brood in association with their gut symbionts. Here, we provide an integrated analysis of the reproduction schedule, gut microbial fluctuations, and resource allocation of founding pairs in incipient colonies of the subterranean termite Reticulitermes speratus. There was a steep increase followed by a rapid decrease in the numbers of intestinal protozoa (hereafter, gut microbial pulse) at the time of larval hatching. Besides the monogamous pairs, same-sex (male-male and female-female) pairs also form tentative colonies in this species. Comparisons of changes in the amounts of protein and lipids in the different pair types revealed that the gut microbial pulse played an essential role in the parental feeding of the first-brood larvae. It was also observed that founders utilized stored protein and lipids for brood production. Our study highlights a significant role for intestinal symbionts during the early stage of the termite life cycle. Propagules of most animals and plants encounter resource limitations after dispersal. This is especially true for termite alates; monogamous pairs found new colonies without help from workers. How do they manage the limited resources, including their nutritional reserves and gut microbes, during colony foundation? To gain a better understanding, we analyzed time-series data of reproduction schedules, gut microbial fluctuations, and resource allocation patterns in the founders of incipient termite colonies. We identified a “gut microbial pulse”, i.e., a steep increase followed by a rapid decrease in the number of intestinal protozoa, that occurred at the time of larval hatching. Our data imply that resources derived from the pulse play an essential role in parental provisioning to larvae. This study highlights a novel strategy of termites to overcome resource-limited situations and successfully establish a new colony.

Etiology of Autism Spectrum Disorders and Autistic Traits Over Time

The frequency with which autism spectrum disorders (ASDs) are diagnosed has shown a marked increase in recent years. One suggestion is that this is partly because of secular changes in the environment, yet to our knowledge this hypothesis lacks evidence. To assess whether the relative importance of genetic and environmental associations with ASD and autistic traits has changed over a 16-year and 26-year period. A twin design was used to assess whether the heritability of ASD and autistic traits has changed over time. Data from 2 nationwide Swedish twin cohorts was used: the Swedish Twin Registry (STR; participants born between January 1982 and December 2008) and the Child and Adolescent Twin Study in Sweden (CATSS; participants born between January 1992 and December 2008). Autism spectrum disorder diagnoses were identified for twins in the STR, with follow-up to 2013. Questionnaires assigned screening diagnoses of ASD to CATSS participants and assessed autistic traits. Analyses were performed from September 1, 2018, to March 31, 2019. Each sample was divided into several birth cohorts covering 1982 to 1991 (for the STR only), 1992-1995, 1996-1999, 2000-2003, and 2004-2008. We assessed whether the genetric and environment variance underlying autistic traits changed across birth cohorts and examined whether the relative contribution of genetics and environment to liability for autism changed across birth cohorts. Data were available for 22 678 twin pairs (5922 female same-sex pairs [26.1%], 5563 male same-sex pairs [24.5%], and 11193 opposite-sex pairs [49.4%]) in the STR and 15 280 pairs (4880 female same-sex pairs [31.9%], 5092 male same-sex pairs [33.3%], and 5308 opposite-sex pairs [34.7%]) in CATSS. The heritability of ASD diagnoses in the STR ranged from 0.88 (95% CI, 0.74-0.96) to 0.97 (95% CI, 0.89-0.99). The heritability of screening diagnoses in CATSS varied from 0.75 (95% CI, 0.58-0.87) to 0.93 (95% CI, 0.84-0.98). Autistic traits showed a modest variance increase over time that was associated with increases in genetic and environmental variance, with the total variance increasing from 0.95 (95% CI, 0.92-0.98) to 1.17 (95% CI, 1.13-1.21) over time. Weak evidence was found for changes in the genetic and environmental factors underlying ASD and autistic traits over time. Genetic factors played a consistently larger role than environmental factors. Environmental factors are thus unlikely to explain the increase in the prevalence of ASD.

Gender Nonconformity of Identical Twins with Discordant Sexual Orientations: Evidence from Video Recordings.

Gender nonconformity (femininity in males, masculinity in females) is more common in non-heterosexual (bisexual and homosexual) than heterosexual individuals. In the past research (Watts, Holmes, Raines, Orbell, & Rieger, 2018b), identical twins discordant for sexual orientation were perceived more similar in their gender nonconformity than unrelated heterosexual and non-heterosexual people. Yet, these perceptions were based on photographs, which do not contain all aspects of gender nonconformity, such as movement and sound. This could have led to an underestimation of observable difference within twin pairs. We examined evaluations of video recordings from adult identical twins with discordant sexual orientations (eight male pairs, 11 female pairs). These twins were a small subset of those who participated in Watts et al. (2018b). Non-heterosexual twins were rated as significantly more gender nonconforming than their heterosexual co-twins, but only when males and females were combined. Their difference was smaller than the analogous difference between identical twins who were concordant heterosexual (three male pairs, three female pairs) and, unrelated to them, identical twins who were concordant non-heterosexual (six male pairs, two female pairs). These patterns were partially confirmed with twins' self-reported gender nonconformity. Shared influences possibly made twins from discordant pairs somewhat similar in their gender nonconformity, even if non-shared factors differentiated their sexual orientations.

Inseminação artificial em codornas através de diferentes métodos de coleta de sêmen

Objetivou-se avaliar diferentes métodos de coleta de sêmen em codornas e a possível viabilidade da inseminação artificial. Foram utilizados dois métodos de coleta: método 1: Massagem abdominal no macho, e método 2: Massagem abdominal na fêmea após a cópula do macho. Foram utilizadas 16 fêmeas e 13 machos da espécie Coturnix coturnix coturnix, com idade de 10 semanas. No primeiro método, o macho selecionado foi estimulado com a técnica massagem abdominal, fazendo-o liberar uma secreção espumosa pelas glândulas para cloacais e seu aparelho reprodutivo, a qual era coletada em uma seringa descartável de insulina de 1 ml, sendo posteriormente aplicada imediatamente na região da vagina de cada dupla de fêmeas do método 1. Já no segundo método 2, foi realizada a cópula do macho selecionado nas fêmeas manequins, onde as mesmas receberam uma massagem abdominal para a retirada do ejaculado do macho. Os ovos de cada método foram marcados e incubados durante 18 dias. Houve um índice de fertilidade de 0% no método 1, e de 4.88% no método 2. Após o insucesso nas taxas de fertilidade e viabilidade, procuraram-se os testes para detecção dos espermatozoides no sistema reprodutivo do macho através de cortes histológicos. Chegou-se à conclusão previa os métodos de inseminação artificial não foram eficientes por não exercerem a pressão necessária para viabilizar o espermatozoide. Dessa forma, os métodos de coleta de sêmen e inseminação artificial aplicado obtiveram índices indesejáveis, logo não são métodos viáveis de manejo reprodutivo em codornas.