Aim. To examine the opinion of patients and nurses about professional appearance in nursing in relation to gender and age and to examine how they perceive professionalism. Methods. Respondents in the cross-sectional study were nurses and patients. An anonymous questionnaire created for the purpose of this research was used to collect data. Results. A total number of 764 respondents participated in the research. Male respondents show significantly more agreement with the statement that the physical appearance of the female nurse (p=0.003) / male nurse (p=0.005) affects the satisfaction with the health care provided. Respondents of older age groups show significantly more agreement with the statement that a female nurse dressed in a skimpier uniform will attract the attention of male patients and that during working hours she should cover the tattoo/piercing, unlike younger respondents. Compared to medical workers, patients show significantly more agreement with the statement that the physical appearance of female nurses is not related to their expertise (p=0.001), and that they would feel more comfortable if they were taken care of by a female nurse that is properly dressed (p=0.05). Both female and male nurses show significantly more agreement with the statement that the uniform should not put them in an uncomfortable situation (p=0.004). Conclusion. Most respondents believe that both female and male nurses should be properly dressed in the workplace and respect the provisions of professional appearance.