Work related musculoskeletal problems and its impacts are present worldwide and currently musculoskeletal problem is mostly affected by transportation services mostly buses. In buses, among conductors and bus drivers most affected are the lady conductors. There are about 33% reserved for the female bus conductors job. Due to this reservation, there are many females working in MSRTC (Maharashtra State Road Transport Corporation). They have to work minimum 8-10 hours per day with less period of rest. Therefore, they have to face many musculoskeletal and psychological problems due to their work schedule which begins from early morning to late night. Our objective is to study the prevalence of work related musculoskeletal and psychological problems among female bus conductors in Karad. Study was conducted using a sample size of 48 female bus conductors for a period of 6 months. The inclusion criteria was female bus conductors who were having more than 5 years of working experience and exclusion criteria was the subjects with musculoskeletal problems detected prior to joining their recruited duties and subjects who were not willing to participateWe found that most of the females are suffering from discomfort in lower back 88% followed by head and neck 56%, Psychologically, 60% from mild depression, 44% from stress and 40% from moderate anxiety suffered. From this study, it can be concluded that these female bus conductors suffer from work related musculoskeletal such as discomfort in major body parts and psychological problems such as depression, anxiety and stress.