This study aimed to compare two female field hockey teams of different competitive levels by analyzing kinematic variables in repeated sprint ability (RSA) tests and the force-velocity profile (FVP). Twenty-five female hockey players representing the elite and subelite levels from the same club volunteered to participate. The RSA protocol included six 30 m maximal sprints with a 30 s recovery. Kinematic variables, such as sprint time, step frequency, and step length, were analyzed for each sprint. Additionally, players performed counter-movement jumps (CMJs) and CMJs with 50% body weight (CMJ50s) to calculate the FV50 using the Bosco Index. The elite players showed better (≈2%; p < 0.05) fatigue indexes in sprint time (0–30 m and 0–10 m sections), step length (0–10 m, 10–20 m, and 20–30 m sections), and step frequency (20–30 m section) during the RSA test, as well as greater values (>10%; p < 0.05) in the CMJ50 and FV50 tests. In addition, these RSA (sprint time, step frequency, and step length) and jumping (CMJ, CMJ50, and FV50) variables showed a moderate, significant, or very significant relationship with each other. Therefore, it seems that both strength and speed capacities can be used either in conjunction or as a complementary approach to enhance the overall RSA performance.