We extend recent conversations on gender differences in entrepreneurship by examining investors’ neural responses to pitches made by male versus female founders. We conduct an experiment that assigns a series of male and female entrepreneur pitches to prospective investors, while undergoing functional magnetic resonance imaging (fMRI) scanning to investigate differential neural activations. We find that investor’s neural activity in the left posterior temporal fusiform cortex is significantly enhanced when presented with pitches from male versus female entrepreneurs. Moreover, using machine learning methods we can reliably decode the gender of the entrepreneur from both multivariate neural activation patterns of investors (71% accuracy) and investing interest (86% accuracy), thereby exposing a possible association between neural mechanisms and behavioral attitudes towards male entrepreneurs. Collectively, this paper is one of the first-known investigations that experimentally advances the neuroscience research agenda in entrepreneurship, uses multivariate pattern analysis as methodology, and sheds new light on the neural-based underpinnings of differential gender evaluations in entrepreneurship.