Female Floral Buds Research Articles

A developmentally regulated GTP binding tyrosine phosphorylated protein A-like cDNA in cucumber (Cucumis sativus L.)

Cucumber (Cucumis sativus) is a monoecious plant that serves as a model for the study of floral sex determination. The genetic background, hormonal and environmental factors regulating unisexual flower development are well characterized, however, the molecular mechanisms are less well understood. To isolate genes involved in male and female flower development we conducted a differential cDNA-Amplified Fragment Length Polymorphism analysis using plant growth apices of predominantly male (monoecious) and female (gynoecious) near isogenic cucumber lines. The plant apices of monoecious cucumbers carry bisexual and unisexual male floral buds while gynoecious ones carry bisexual and unisexual female floral buds. We isolated a cDNA fragment that encodes a putative GTP binding tyrosine phosphorylated protein A (CsTypA1) that is developmentally regulated. CsTypA1 is expressed in stamen primordia and its transcript is more abundant in monoecious plant apices implying a role for CsTypA1 in the early stages of male reproductive organ development. At later stages of flower development a higher transcript level is observed in female flowers in stigmatic papilla, nectary and in particular ovule/ovary tissue. The differential expression of CsTypA1 during male and female flower development indicates a role for CsTypA1 in female flower development, in particular that of the ovary/ovule. Thus, CsTypA1 might have a dual role, one in the early stages of flower development, possibly during sex determination, and the other in the development of the ovary/ovule. This is the first report of a gene encoding a putative TypA in the plant kingdom that is differentially expressed during plant development.

Isolation of a partial sequence of a putative nucleotide sugar epimerase, which may involve in stamen development in cucumber (Cucumis sativus L.)

Sex determination is the most widely studied subject in cucumber. The sex of cucumber plants can be monoecious, hermaphrodite, gynoecious, androecious, or andromonoecious. Besides environmental factors, three major genes, F/f, M/m, and A/a mainly govern the sex types in cucumber. Regardless of their sex all floral buds are bisexual at the early bud stage. A stage specific arrest of either stamen or carpel leads to unisexual flower development. The possible downstream product of the interaction of the sex determining genes that may directly allow the growth or selectively arrest stamen or pistil is not yet identified. Therefore, in the current study, we performed suppression subtractive hybridization using floral buds from nearly isogenic gynoecious and hermaphrodite cucumber plants and identified for the first time a cDNA homologous to nucleotide sugar epimerase. The expression level of the isolated putative nucleotide sugar epimerase is weak in female floral buds but strong in bisexual and male flowers. The weak level of the putative nucleotide sugar epimerase may be an indication for its improper functioning, which may influence stamen development in cucumber plants.

Shoot ontogeny in Fraxinus pennsylvanica (green ash). II. Development of the inflorescence

From initiation to fruit set, which occurs over three growing seasons, eight stages are recognized in the development of axillary inflorescences in the dioecious species Fraxinus pennsylvanica var. subintegerrima (Vahl) Fern, (green ash). In the first season, buds are initiated in the axils of foliage leaves. As the shoots expand in the following spring, the buds complete their development. Although similar at first, differences begin to emerge between vegetative and inflorescence buds in that the latter produce robust second-order meristems, the incipient paracladia, protruding close to the original apex. After about 3–4 weeks, when the initiation of such buds is complete, the terminal and subtending lateral meristems present on each axis develop into a three-membered cluster of floral buds. There was a mean of 214.3 ± 12.2 floral buds initiated per female inflorescence, and the number generally increased with the length of the associated shoot. A ridge, the incipient perianth, begins to form around the periphery of each rounded floral apex. Male and female floral buds are not distinguishable at this stage, but the inflorescence buds are distinctly different from vegetative buds. The male and female buds then diverge in their development in that an identation forms at the summit of the incipient gynoecium and male buds initiate two or three anthers. By autumn, the gynoecium is distinctly conical, with an orifice at its summit, and the anthers are lobed. There is lobing of the perianth ridge, but in the mature flower distinct organs traceable to such lobes could not readily be identified.

Sex expression in floral buds of Acorn squash

Staminate flowers of the Acorn squash (Cucurbita pepo, cv. Table Queen) are initiated monosexually, pistillate flowers are initiated bisexually. Pistillate flower primordia are physiologically bisexual from a stage below 0.3 mm until they are 0.8 mm in width. Male and female floral buds were cultivated on White's medium, modified according to Galun et al. (1963). In excised female floral buds the relative development of stamens is better than in in-situ floral buds; the opposite relationship holds for ovary development. The smaller the bud at excision, the greater the stamen growth and the lesser the ovary growth, both in absolute and relative terms. Although smaller pistillate floral buds cultured in vitro showed a greater increase in maleness than larger ones, there was no difference in the percentage of female buds between explants of the two sizes when originating from the “mixed” stem region (that is, the stem region carrying male and female flowers). Addition of 3-indoleacetic acid and the anti-auxin p-chlorophenoxyisobutyric acid to standard medium in equal amounts resulted in somewhat better development of excised floral buds than addition of each of these compounds alone. The percentage of female buds among explants originating from identical nodes was not significantly influenced by 3-indoleacetic acid or p-chlorophenoxyisobutyric acid.