Objective: To analyze the contributions of female fishers to the preservation of knowledge about local artisanal fishing, as well as the challenges they face in occupying these spaces. Introduction and Theoretical Framework: Data on the importance of artisanal fishing is presented, highlighting its relevance in the intergenerational transmission of this knowledge by women. It also emphasizes the neglect and invisibility faced by women throughout history due to gender issues, which led to women achieving basic rights granted to "fishermen" only belatedly. Method: The methodology adopted includes field data collection through semi-structured interviews with female fishers from the community, as well as direct observations. The interviews were conducted using the snowball sampling method, as described by Bailey (1994). The interviews were recorded and accompanied by photographic records, with proper authorization obtained through the signing of the Informed Consent Form (ICF), which was read and explained to each participant. Results and Discussion: The results show that female fishers possess extensive knowledge about fishing techniques and equipment, fishing resources, and other environmental issues. These women face a triple workload and many difficulties in estuarine environments. They recognize the sea and mangroves as prosperous areas that allow them to raise their children and obtain resources for their subsistence. Fishing becomes more than just an economic activity; it shapes the identity and ways of being/living/inhabiting of these women. Thus, the quilombola women of Santiago do Iguape take on a leading role in an activity marked by gender issues and the pursuit of basic rights. Implications of the Research: The research contributes to the recognition of fishing knowledge built by female fishers. These discussions may contribute to the improvement and implementation of new policies that address the current vulnerabilities faced by these fishers. Originality/Value: This study is essential for highlighting social and governmental invisibility due to gender issues, as well as the changes in women’s self-perception regarding their importance in the construction and preservation of knowledge about artisanal fishing and their rights.