Female Asian Elephants Research Articles

BUILDING AN ELEPHANT BRAIN DATABASE: ADVANCING INSIGHTS INTO COGNITIVE AGING IN ELEPHANTS

Abstract Cognitive aging is a complex phenomenon, and a comparative approach leverages the strengths of various species to identify conserved and divergent mechanisms associated with cognitive health. Elephants are an informative species for cognitive aging research because they are analogous to humans in that they live to comparable ages, display complex cognition, have strong long-term memory, and rely on social bonds. They differ, however, in their evolutionary path to arrive at these same outcomes. Studying elephants can potentially reveal different trajectories for cognitive performance maintenance and impairment compared to humans, and provide information on brain aging in elephants, contributing missing knowledge on aspects of well-being in a long-lived species. We initiated the Elephant Brain Database to enhance the use of elephants as a comparative species to further understand the structure, function, and evolution of the brain, and to increase the availability of elephant postmortem brain tissue, MRI scans, and related datasets for investigators. This repository is for postmortem fixed and frozen brain specimens and when possible, postmortem structural MRI scans and diffusion tensor images. In collaboration with North American zoos and partners in Zambia, we obtained fixed or frozen brain specimens from 23 individuals (age: stillborn to 75 years). Of those, 12 are from Asian elephants (Elephas maximus; 8 females, 4 males) and 11 are from African savanna elephants (Loxodonta africana; 4 females, 7 males). A structural MRI scan was conducted on one left hemisphere from a 52-year-old female Asian elephant. This collection represents the largest consolidation of elephant brain resources.

Physical activity and temperature changes of Asian elephants (Elephas maximus) participating in eco-tourism activities and elephant polo.

Captive and domestic animals are often required to engage in physical activity initiated or organised by humans, which may impact their body temperature, with consequences for their health and welfare. This is a particular concern for animals such as elephants that face thermoregulatory challenges because of their body size and physiology. Using infrared thermography, we measured changes in skin temperature associated with two types of physical activity in ten female Asian elephants (Elephas maximus) at an eco-tourism lodge in Nepal. Six elephants took part in an activity relatively unfamiliar to the elephants-a polo tournament-and four participated in more familiar ecotourism activities. We recorded skin temperatures for four body regions affected by the activities, as well as an average skin temperature. Temperature change was used as the response variable in the analysis and calculated as the difference in elephant temperature before and after activity. We found no significant differences in temperature change between the elephants in the polo-playing group and those from the non-polo playing group. However, for both groups, when comparing the average skin body temperature and several different body regions, we found significant differences in skin temperature change before and after activity. The ear pinna was the most impacted region and was significantly different to all other body regions. This result highlights the importance of this region in thermoregulation for elephants during physical activity. However, as we found no differences between the average body temperatures of the polo and non-polo playing groups, we suggest that thermoregulatory mechanisms can counteract the effects of both physical activities the elephants engaged in.

Do food distribution and competitor density affect agonistic behaviour within and between clans in a high fission-fusion species?

According to the ecological model of female social relationships (EMFSR), within-group competition and between-group competition in female-bonded species are shaped by food distribution. Strong between-group contests are expected over large, monopolizable resources and high population density, but not when low-quality food is distributed across large, undefended home ranges. Within-group contests are expected to be more frequent with increasing heterogeneity among feeding sites and with group size. We tested these predictions in female Asian elephants, which show traits associated with infrequent contests-graminivory, high fission-fusion and overlapping home ranges. We examined how food distribution and competitor density affected agonistic interactions within and between female elephant clans (social groupings) in the Kabini grassland, southern India. We found stronger between-clan contest in the grassland than that known from neighbouring forests, and more frequent agonism between females between clans than within clans. Such strong between-clan contest was attributable to the grassland being a food-rich habitat patch, thus supporting the EMFSR. Within-clan agonism was also frequent, but did not increase with food heterogeneity, contradicting the EMFSR. Contrary to recent claims, increasing within-clan agonism with group size suggested ecological constraints on large groups despite high fission-fusion. High population density may explain such frequent contests despite graminivory and fission-fusion.

Distribution and ranging area of elephant in Simpan Panti Forest, Kota Tinggi, Johor, Malaysia

Abstract. Hassan NHN, Abdullah NM, Norazlimi NA, Saaban S, Yazi MFA. 2023. Distribution and ranging area of elephant in Simpan Panti Forest, Kota Tinggi, Johor, Malaysia. Biodiversitas 24: 5731-5738. Home range estimation is one of the most used animal ecology studies using a Geographic Information System (GIS). One adult female Asian elephant (Elephas maximus Linnaeus, 1758) was fitted with a telemetry satellite collar African Wildlife Tracking (AWT) to determine its home range size distribution through its ranging behaviour and to identify the relationship between the GPS collar location record and the area covered. The home range estimation was determined using GPS collar location data from a satellite telemetry system and analyzed by using the Minimum Convex Polygon methods. GPS collar location data were obtained from the Department of Wildlife and National Parks (DWNP). The movement of elephant data was overlayed with the land use data of the Simpan Panti Forest (Hutan Simpan Panti), Kota Tinggi District, Johor, Malaysia before being exported to ArcGIS Pro software. The ArcGIS Pro software can estimate this Asian elephant home range sizes by using the MCP method for twelve months, from 1 June 2019 until 31 May 2020. The total home range size is 447.17 km². Home range size is estimated to be 31.77 to 303.86 km² in one year. The size of this home range has presumably been impacted by extensive human activities such as highways and settlement areas. So, good and strong management is needed to control this conflict for humans to live in harmony in one area and protect the remaining forest reserve area from expanding to agricultural activity and human development areas.

3D Reconstruction of the Blood Supply in an Elephant's Forefoot Using Fused CT and MRI Sequences.

Being the largest still-living terrestrial mammal on earth, an elephant's feet play an important role in its health status. The musculoskeletal structures in the forefoot are well described in the literature, but information about vascularization is limited. The novel aim of this work is to provide anatomical guidance to structures found in the forefoot, focusing on the arterial system. Initially, native CT and MRI sequences were taken of the left forefoot of a deceased 6-year-old female Asian elephant; the foot was then filled with an iodine-containing contrast medium through the a. mediana and the CT scans were repeated in the same position. The images obtained were processed with 3D Slicer software for the 3D reconstruction of the bones and arteries. The results clearly showed the palmar blood supply of the forefoot. A so far undescribed vessel was revealed, stemming from the a. metacarpea, supplying the first digit and the digital cushion. The course of the deep palmar arch's terminal section was also established. This paper provides the first description of the exact disposition of the arteries in the palmar aspect of an elephant's forefoot and may be used in planning surgeries in clinically affected animals.

CORNEAL STROMAL ABSCESS IN A CAPTIVE ASIAN ELEPHANT: DIAGNOSIS AND TREATMENT REGIMES

Ocular injuries are often observed in elephants, most due to accidents. Diagnostic and treatment are challenging, as the elephants usually keep their eyes closed during examinations. This article describes the diagnosis and treatment regime of a juvenile captive female Asian elephant (Elephas maximus; approximately four years old) that showed signs of severe epiphora, blepharospasm, and corneal opacity in her left eye. Ophthalmic examination using a slit lamp and ocular ultrasonography revealed a corneal stromal abscess. Intensive treatment was performed, including administering intravenous antibiotics and anti-inflammatory drugs and an hourly eye topical treatment. The treatment regime was adjusted from time to time following the clinical signs and diagnostic results. The abscess was invisible within approximately five weeks (day 37). The treatment was continued until day 74 and then ceased. The eye recovered entirely, and only a small scar remained at the center.

Virtual fencing of captive Asian elephants fitted with an aversive geofencing device to manage their movement

Aversive Geofencing Devices (AGDs) are designed to emit audible warning signals followed by electric shocks when animals reach virtual fences (VFs) with the intent that animals will learn to turn away at audio warnings and thereby avoid receiving shocks. AGDs are a potentially useful tool for mitigating human-elephant conflict, but a greater understanding of captive elephant responses to AGDs is required before they might be confidently used on wild elephants. We conducted experiments with eight, female captive Asian elephants using a modified dog-training collar to deliver mild electric shocks (4 kV) of varying strength (pulse frequencies) to determine the ideal location on the neck to deliver the stimuli and the optimum strength of the shock required to generate desired aversive responses. Ten shocks (<1 s duration) of different strengths were delivered during a 10 min session (i.e., one shock per minute) at two positions on one side of the elephant’s neck. Results indicated that elephants were more likely to display desirable aversive behaviours at the upper position tested on the neck (odds ratio=0.47, 95% CI 0.25–0.87, P = 0.018) and at higher stimuli strengths (odds ratio=1.03, 95% CI 1.01–1.04, P ≤ 0.001). A conditioning experiment was then conducted several months later with five of the same elephants. These were individually trained to walk along a ∼100 m path to a food reward on three consecutive days, wearing a dummy collar. On the next three days and on one other day few months later, the elephants were fitted with a similar shock collar (positioned at the upper neck location, and with the highest strength tested earlier) to determine if the AGD could prevent the elephants from accessing the food reward. Three VFs were established at ∼30 m, ∼50 m and ∼60 m points along the path. As the elephant approached the food, a mild audio warning, a more aggressive audio warning, and an electric shock was administered at the first, second and third VFs respectively. Warnings and shocks were not delivered if elephants heeded earlier warnings. A maximum of five such trials were attempted. The VFs successfully kept elephants from reaching the food 77.8% of the time, with elephants responding to the audio warnings and avoiding electric stimulation 47.2% of the trials. These findings suggest that AGDs are a promising method to manage elephant movement, but further research is needed to develop a reliable approach for wild elephants.

Surgical Removal of Urinary Bladder Urolith in Female Asian Elephant (Elephas maximus) by Episiotomy and Urinary Bladder Sphincterotomy

Surgical Removal of Urinary Bladder Urolith in Female Asian Elephant (Elephas maximus) by Episiotomy and Urinary Bladder Sphincterotomy

Cover Picture and Issue Information

Journal of EcologyVolume 110, Issue 2 p. 279-281 COVER PICTURE AND ISSUE INFORMATIONFree Access Cover Picture and Issue Information First published: 09 February 2022 https://doi.org/10.1111/1365-2745.13697AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Graphical Abstract Cover image: Female Asian elephant (Elephas maximus) foraging in a dipterocarp forest of Peninsular Malaysia. Large and threatened vertebrates like elephants, gibbons, and hornbills, play irreplaceable roles in ecosystem processes, such as seed dispersal, that are rarely evaluated at the community level. Photograph by Lisa Ong. For full details see pages 313–326. Volume110, Issue2February 2022Pages 279-281 RelatedInformation

Characteristics of social relationships in a group of captive Asian elephants (Elephas maximus) in the elephant village in Thailand

Wild Asian elephants (Elephas maximus) form complex fission–fusion societies centered on matrilineal groups. In captivity, unrelated females remain in the same group and create social relationships. To better understand well-being in captivity, it is important to investigate the social relationships between females. However, to date, little information is available on this topic. The goal of this study was to clarify the social relationships between captive female Asian elephants using social network analysis. Our subjects were 13 captive Asian elephants at the Elephant Study Center in Surin Province, Thailand. We investigated variations in the frequencies of affiliative behaviors between dyads. Individuals that stayed in the group longer tended to play a more central role in terms of group member connections. We found that two individuals played an important role in strengthening connections, and that their removal influenced group cohesion. Our results revealed that individuals that stayed in the group long-term and that had existing relationships with others in the group tended to build better social connections, regardless of their age. In addition, the existence of some young individuals that show frequent affiliative behaviors may be important for bond strength and therefore, overall group cohesion.

Acoustic structure and information content of trumpets in female Asian elephants (Elephas maximus).

Most studies on elephant vocal communication have focused on the low-frequency rumble, with less effort on other vocalization types such as the most characteristic elephant call, the trumpet. Yet, a better and more complete understanding of the elephant vocal system requires investigating other vocalization types and their functioning in more detail as well. We recorded adult female Asian elephants (Elephas maximus) at a private facility in Nepal and analyzed 206 trumpets from six individuals regarding their frequency, temporal and contour shape, and related acoustic parameters of the fundamental frequency. We also tested for information content regarding individuality and context. Finally, we recorded the occurrence of non-linear phenomena such as bifurcation, biphonation, subharmonics and deterministic chaos. We documented a mean fundamental frequency ± SD of 474 ± 70 Hz and a mean duration ± SD of 1.38 ± 1.46 s (Nindiv. = 6, Ncalls = 206). Our study reveals that the contour of the fundamental frequency of trumpets encodes information about individuality, but we found no evidence for trumpet subtypes in greeting versus disturbance contexts. Non-linear phenomena prevailed and varied in abundance among individuals, suggesting that irregularities in trumpets might enhance the potential for individual recognition. We propose that trumpets in adult female Asian elephants serve to convey an individual’s identity as well as to signal arousal and excitement to conspecifics.

Clinical approach to colic and collapse in an Asian elephant (Elephas maximus) withSalmonellasaintpaul septicaemia and subsequent ileus

AbstractAn adult female Asian elephant (Elephas maximus) presented with clinical signs of colic unresponsive to analgesia, which progressed to hypothermia and collapse within 48 hours. Repeated sedations using butorphanol and detomidine were performed for initial diagnostic sampling, first aid and subsequent treatment. Initial haematology showed evidence of septicaemia and disseminated intravascular coagulation; urine analysis was consistent with metabolic acidosis. The initial treatment focused on rectal administration of enrofloxacin, metronidazole and fluids. By Day 7, the immune system was recovering as demonstrated by blood parameters but ileus had developed. Sedation interventions were discontinued and treatment consisted of oral ranitidine, fibre provision and rehydration.Salmonellasaintpaul was cultured from the faeces and a disease risk analysis identified a possible infection route through food contamination. Serial haematology provided direction in clinical decision making throughout this challenging case.

Reproductive tract neoplasia in adult female Asian elephants (Elephas maximus).

Recent reports have highlighted a lower-than-expected prevalence of neoplasia in elephants and suggested mechanisms for cancer resistance. But despite infrequent reports in the literature, uterine neoplasia is common in managed Asian elephants (Elephas maximus). This study is an archival review of reproductive tract neoplasia in 80 adult female Asian elephant mortalities in managed care facilities in the United States from 1988 to 2019. Neoplasms occurred in 64/80 (80%) of cases. Most were in the uterus (63/64; 98%) with only a single case of ovarian neoplasia. Myometrial leiomyomas were present in 57/63 (90%) cases with uterine neoplasia. Uterine adenocarcinoma was present in 8/63 (13%) cases. Remaining cases included endometrial adenoma (2), focal carcinoma in situ in endometrial polyps (1), anaplastic carcinoma (1), endometrial hemangioma (1), primitive neuroectodermal tumor (PNET; 1), and angiosarcoma (1). One case with uterine adenocarcinoma had a separate pelvic mass histologically characterized as an anaplastic sarcoma. Distant metastases were documented in 5/8 (63%) cases of uterine adenocarcinoma, and in the uterine anaplastic carcinoma, PNET, and angiosarcoma. Four uterine adenocarcinomas and one carcinoma in situ were examined immunohistochemically for pan-cytokeratin, vimentin, and estrogen receptor. In all, neoplastic cells were pan-cytokeratin positive and vimentin negative, and in 2 cases were immunoreactive for estrogen receptor. Results show that female reproductive tract neoplasia, particularly of the uterus, is common in Asian elephants and is not limited to leiomyomas. Importantly, uterine neoplasms have the potential to impact fecundity and may represent obstacles to conservation in managed care.

Effects of physiological changes and social life events on adrenal glucocorticoid activity in female zoo-housed Asian elephants (Elephas maximus).

Ensuring good health and welfare is an increasingly important consideration for conservation of endangered species and includes breeding of individuals managed under human care. Understanding how factors in the captive environment affect individual animal wellbeing can be aided by long-term monitoring of biological functioning. This study involved longitudinal assessments (4 to 28 years) of reproductive and adrenal hormones in zoo-housed female Asian elephants (Elephas maximus) (age range 4 to ~71 years) to elucidate patterns in adrenal glucocorticoid (GC) activity in association with reproductive and demographic factors, and examine individual response to major social changes. Concentrations of serum and urinary cortisol covaried more consistently with physiological changes (ovarian cycle phase, puberty, pregnancy, lactational anestrus, and age) than with social life events (births, deaths, and facility transfers). Cortisol fluctuated across the ovarian cycle with mean concentrations being higher in the follicular than in the luteal phase, and concentrations were highest in lactational anestrous compared to all other reproductive states. The elephants in this study exhibited substantial individuality in adrenal GC response to major social change, reinforcing the need to assess welfare on an individual basis and to consider factors influencing the impact of perceived stressors, such as social relationships, social support, temperament, and life history. Outcomes from this study deepen our understanding of Asian elephant physiology and highlight the importance of taking intrinsic patterns of hormone secretion into account when evaluating the impact of external factors. Finally, a better understanding of the impact of social change and resiliency in response to real and perceived stressors allows us to improve social management to enhance welfare in both captive settings and free-ranging environments.

Utilizing Camera Traps, Closed Circuit Cameras and Behavior Observation Software to Monitor Activity Budgets, Habitat Use, and Social Interactions of Zoo-Housed Asian Elephants (Elephus maximus).

Simple SummaryZoological institutions place strong emphasis on monitoring the welfare of individual animals in their care. Long-term behavior data can be integrated into zoological management to allow for adjustments on a continuous basis as animals respond over time to social, environmental, or physical changes. Behavioral observations conducted for two years on one male and six female Asian elephants (Elephas maximus) using Zoo Monitor, a closed-circuit camera system, and camera traps revealed individual activity budgets, habitat use and social interactions in ways beyond use of these tools in isolation. This multi-tool approach provides a broader assessment of individual welfare, which can lead to better management of elephants, and potentially other species, in zoological settings.Accredited zoos and aquariums value superior animal husbandry and strive to ensure that the physical, psychological, and social needs of animals are met. In North America, the Association of Zoos and Aquariums (AZA) relies on species-specific standards to ensure facilities provide the best care for collection animals. The AZA also makes explicit recommendations for long-term monitoring of welfare. Data collected through behavioral observations can be used to modify management as animals respond over time to social, environmental, or physical changes. In long-lived, social species like elephants, it is particularly important to document herd dynamics, calf development, geriatric health, and social bonds throughout their lifetimes. The Smithsonian’s National Zoological Park housed one male and six female Asian elephants in dynamic social groupings. Behavioral observations were conducted on all elephants for two years using two methods involving ZooMonitor, closed circuit cameras, and camera traps. The goal was to compare how these two methods function to provide individual activity budgets, habitat use, and social interactions. Methodologies such as these, alone or in combination, have the potential to produce valuable data about potential changes in welfare over time in a zoological setting and can be performed either by staff or volunteers with high reliability.

Changes in age-structure over four decades were a key determinant of population growth rate in a long-lived mammal.

A changing environment directly influences birth and mortality rates, and thus population growth rates. However, population growth rates in the short term are also influenced by population age-structure. Despite its importance, the contribution of age-structure to population growth rates has rarely been explored empirically in wildlife populations with long-term demographic data. Here we assessed how changes in age-structure influenced short-term population dynamics in a semi-captive population of Asian elephants Elephas maximus. We addressed this question using a demographic dataset of female Asian elephants from timber camps in Myanmar spanning 45years (1970-2014). First, we explored temporal variation in age-structure. Then, using annual matrix population models, we used a retrospective approach to assess the contributions of age-structure and vital rates to short-term population growth rates with respect to the average environment. Age-structure was highly variable over the study period, with large proportions of juveniles in the years 1970 and 1985, and made a substantial contribution to annual population growth rate deviations. High adult birth rates between 1970 and 1980 would have resulted in large positive population growth rates, but these were prevented by a low proportion of reproductive-aged females. We highlight that an understanding of both age-specific vital rates and age-structure is needed to assess short-term population dynamics. Furthermore, this example from a human-managed system suggests that the importance of age-structure may be accentuated in populations experiencing human disturbance where age-structure is unstable, such as those in captivity or for endangered species. Ultimately, changes to the environment drive population dynamics by influencing birth and mortality rates, but understanding demographic structure is crucial for assessing population growth.

Circadian Rhythm of Salivary Immunoglobulin A and Associations with Cortisol as A Stress Biomarker in Captive Asian Elephants (Elephas maximus)

Simple SummarySalivary immunoglobulin A (sIgA) and cortisol concentrations were measured in Asian elephants to determine circadian rhythm effects and the relationship between both biomarkers. Saliva samples were collected every 4 h from 06:00 to 22:00 h for 3 consecutive days (n = 15 samples/elephant). We used enzyme immunoassays for quantification of sIgA and cortisol concentrations. Both sIgA and cortisol followed a circadian rhythm, although the patterns differed. For both, the highest concentrations were in the early morning hours when elephants began the work day; however, sIgA concentrations were more variable during the day. There was no correlation between the two indices because the pattern of sIgA was quartic, while that of cortisol was linear. We provide basic knowledge for further studies using sIgA as a welfare biomarker.Salivary immunoglobulin A (sIgA) has been proposed as a potential indicator of welfare for various species, including Asian elephants, and may be related to adrenal cortisol responses. This study aimed to distinguish circadian rhythm effects on sIgA in male and female Asian elephants and compare patterns to those of salivary cortisol, information that could potentially have welfare implications. Subjects were captive elephants at an elephant camp in Chiang Mai province, Thailand (n = 5 males, 5 females). Salivette® kits were used to collect saliva from each elephant every 4 h from 06:00 to 22:00 h for 3 consecutive days (n = 15 samples/elephant). Enzyme immunoassays were used to quantify concentrations of IgA and cortisol in unextracted saliva. Circadian rhythm patterns were determined using a generalized least-squares method. Both sIgA and cortisol followed a circadian rhythm, although the patterns differed. sIgA displayed a daily quartic trend, whereas cortisol concentrations demonstrated a decreasing linear trend in concentrations throughout the day. There was no clear relationship between patterns of sIgA and salivary cortisol, implying that mechanisms of control and secretion differ. Results demonstrate for the first time that circadian rhythms affect sIgA, and concentrations follow a daily quartic pattern in Asian elephants, so standardizing time of collection is necessary.

Clinical Management of a Complete Gastrointestinal Obstruction and Ileus in A Geriatric Female Asian Elephant (Elephas maximus)

A 58-year-old female Asian elephant (Elephas maximus) was diagnosed with a complete gastrointestinal obstruction and accompanying ileus. She was anorexic for three days, did not pass stool for seven days, and her abdomen was visibly distended. Treatment included: rectal and IV fluids; oral and rectal stool softeners; mineral oil; ketoprofen; laser acupuncture; and fecal transfaunation. The blockage was successfully expelled after seven days yielding a large bolus weighing 52 kg followed shortly by another 45 kg of feces.

Capture from the wild has long-term costs on reproductive success in Asian elephants.

Capturing wild animals is common for conservation, economic or research purposes. Understanding how capture itself affects lifetime fitness measures is often difficult because wild and captive populations live in very different environments and there is a need for long-term life-history data. Here, we show how wild capture influences reproduction in 2685 female Asian elephants (Elephas maximus) used in the timber industry in Myanmar. Wild-caught females demonstrated a consistent reduction in breeding success relative to captive-born females, with significantly lower lifetime reproduction probabilities, lower breeding probabilities at peak reproductive ages and a later age of first reproduction. Furthermore, these negative effects lasted for over a decade, and there was a significant influence on the next generation: wild-caught females had calves with reduced survival to age 5. Our results suggest that wild capture has long-term consequences for reproduction, which is important not only for elephants, but also for other species in captivity.

To treat or not to treat?

In a world of ever-increasing liability, the decision not to treat a patient comes less and less easily, even though both evidence-based medical and financial reasons may speak against treatment....