Female Age Research Articles

Global burden and trends of disability‐adjusted life years and mortality for decubitus ulcer: A systematic analysis

AbstractThe management of chronic wounds has presented a significant dilemma, which is evident not only in clinical treatment but also in the substantial burden it places on medical resources. The global COVID‐19 pandemic in 2020 is likely to further exacerbate this trend. Therefore, it is imperative to delve deeper into the impact of chronic wound on disease burden across different regions and populations. In this study, we focused on decubitus ulcers (DU) as representative chronic wounds and utilized data from the Global Burden of Disease (GBD) 2019 database (http://ghdx.healthdata.org/gbd-results-tool) pertaining to age, gender, region, year and socio‐demographic index (SDI) group. Disability‐adjusted life years (DALYs) and mortality were utilized as indicators to assess the burden of DU. The analysis and visualization were performed using R software (version 4.2.3). A decrease in the global ASRs of DALYs and mortality for DU was observed across most regions between 1990 and 2019. The reduction in burden was particularly significant in regions characterized by a high SDI, while regions with a high‐middle SDI experienced an increase. The burden of DU increased with age for both males and females, with males generally experiencing a higher burden compared to females. Strengthening population‐based data on the prevalence of DU and implementing dynamic monitoring at the public health level will enable policymakers to develop evidence‐based strategies for efficient allocation of healthcare resources.

Metabolic syndromes increase significantly with the accumulation of bad dietary habits.

The association between dietary habits and metabolic syndrome (MetS) has not been well documented, due to the complexity and individualization of dietary culture in the Chinese population. To construct a composite score from various bad dietary habits and to evaluate their comprehensive association with the prevalence of MetS and its components among Chinese men and women across various age groups. Serial cross-sectional studies. Twenty-three dietary habits were assessed through face-to-face interviews with 98,838 males and 83,099 females in health check-up programs from 2015 to 2021, among which eighteen bad dietary habits were observed to be associated independently with total MetS. The total score of bad dietary habits was composed of four categories via variable clustering analysis, including irregular dietary habits, unhealthy dietary flavors, unbalanced dietary structure, and high-fat diet. The 2016 Chinese guideline for the management of dyslipidemia in adults was used to define MetS. Men had a higher score of bad dietary habits than women (9.63 ± 3.11 vs. 8.37 ± 3.23), which decreased significantly with increasing age in both males and females (Pinteraction<0.01). The prevalence of total MetS increased significantly with the cumulative score of bad dietary habits in both males (highest quintile vs. lowest quintile: OR, 1.90; 95% confidence interval [CI], 1.80-2.00; Plinear<0.01) and females (OR, 2.23; 95% CI, 2.02-2.46; Plinear<0.01) after adjusted for age, education, smoking status, alcohol consumption, and physical activities. These linear trends were also observed for each MetS component (all Plinear<0.01). The role of irregular dietary habits and high-fat diet on MetS prevalence are much higher in males than in females, while unhealthy dietary flavors and unbalanced dietary structure had a greater influence on females. The accumulation of bad dietary habits contributes to the MetS developments. Thus, individualized lifestyle interventions are needed to correct bad dietary habits with regard to gender differences.

Reshaping Gendered Narratives: Reinterpreting Female Art, Identity and Social Change in the Late Nordic Bronze Age

This paper explores the changes to art on artefacts attributed to females in the Late Nordic Bronze Age (ca. 1100–500 BC) from a gender critical, feminist perspective. Traditionally, Scandinavian research has focussed on the art of male artefacts, which is believed to represent a cosmological narrative, whilst female art has been considered devoid of cosmological motifs – concomitantly, it is often assumed that prominent social standing was reserved males. Through analytical discussion, the paper shows how the same motifs as are considered cosmological in male objects can be found on female objects, too, in compositions diverging from the male use, suggesting a gender differentiated use of art and association to cosmology. Through a gender theoretical lens, the paper explores what the social use of the art on these objects may suggest regarding identity and power relations in society, linking up with a reconfiguration of female identity at this time.

Sex‐based differences in track running distances of 100, 200, 400, 800, and 1500m in the 8 and under and 9–10‐year‐old age groups

AbstractThere are contradictory claims regarding sex‐based differences in athletic performance before puberty, but there has been minimal evaluation of sex‐based differences in competitive running performance before puberty. The purpose of this project was to determine if there are prepubertal sex‐based differences in track running performance. Finalist times from the USA Track and Field National Youth Outdoor Championships and National Junior Olympic Championships during the years 2016–2023 for running distances of 100, 200, 400, 800, and 1500m in the 8 and under and 9–10‐year‐old age groups were analyzed for sex‐based differences. In the 8 and under age group, the males were, on average, faster (p = 0.000, Hedges' g = 0.632–0.834) than females by 4.0% in the distance of 100m, 4.7% in 200m, 5.3% in 400m, 6.7% in 800m, and 6.1% in 1500m. In the 9–10‐year‐old age group, the males were, on average, faster (p = 0.000, Hedges' g = 0.584–1.089) than females by 2.9% in the distance of 100m, 4.6% in 200m, 4.0% in 400m, 4.0% in 800m, and 5.9% in 1500m. In each distance and age group between 2016 and 2023, the individual fastest male was faster than the individual fastest female by 3.7 ± 2.3%. The present data indicate that, in elite competition, males in the 8 and under and 9–10‐year‐old age groups typically run faster than females of the same age by 2.9%–6.7% for running distances of 100, 200, 400, 800, and 1500m. These findings are of particular importance as government agencies and sports organizations consider policies regarding single sex sports competition for youth.

Abstract WMP59: Prognosis After Cerebral Venous Thrombosis: Long-Term Readmission Patterns in a Canadian Population-Based Cohort

Background: Information on long-term care utilization patterns following the acute care period of cerebral venous thrombosis (CVT) is limited. We examined readmission reasons and patterns after discharge from index CVT hospitalization. Methods: A population-based cohort of patients diagnosed with CVT (2000-2017) was constructed using linked administrative data. Descriptive statistics were calculated for baseline demographics and readmission causes. Readmission proportions were calculated for first readmission every year during the 10 years after diagnosis. Survival analysis was used to examine associations between patient factors and readmission events. Results: The cohort included 508 patients, 56% female and mean age 49.6 years (SD 19). Median time-to-first readmission is 1.8 years (95% CI 1.4-2.4), with 39% of patients readmitted in 1 year (95% CI 35-43) and 69% in 5 years (95% CI 64-73). Cancer-related CVT was associated with a higher rate of readmission (p&lt;0.0001) and traumatic CVT was associated with a lower rate (p=0.005). In patients younger than age 50 (mean: 36.1 years, SD 9), headache and epilepsy were among the top five reasons for readmission. For patients older than 50 years (mean: 67.6 years, SD 11), cerebral infarction was among the top five reasons. Readmission rate stabilized over time. Conclusion: Cancer-related CVT was associated with significantly higher readmissions and traumatic CVT had significantly lower readmissions. The most common neurologic reasons for readmission differed by age group, but included headaches, seizures, and stroke. Readmission rates were highest in the first two years following discharge but stabilized over time.

Exploring Mental Health Literacy and Its Associated Factors: A National Cross-Sectional Study in Saudi Arabia, 2023.

The aim of this study is to examine, for the first time, the mental health literacy of the Saudi general population. Additionally, we aim to identify demographic factors associated with elevated mental health literacy scores. A cross-sectional study using a phone interview survey with quota sampling was conducted for equal distribution of sexes, age and administrative regions. Mental health literacy was assessed by the previously validated mental health literacy scale (MHLS). The MHLS ranges from 35 to 160, and a higher score indicates greater level of mental health literacy. In this study, 4,547 (70.2%) agreed to complete the interview. The sample was equally distributed across Saudi Arabia's 13 administrative regions with 50.1% female and mean age 35.9 (SD: ±12.8). In terms of education, 55.1% have a bachelor's degree or above. The nationally weighted score on the original MHLS was 110.75. the MHLS Saudi validation score was influenced by various variables including: marital status, healthcare-related job, sex, education level, and proximity to individuals with mental health issues. The study offers data for the first time on Saudi Arabian mental health literacy status. The high MHLS score reflects good societal awareness and understanding of mental health related concepts and issues in the Saudi population. The results of this study set the baseline for mental health literacy in Saudi Arabia. Future research may focus on utilizing the validated MHLS and the method used in this study to explore the effect of other social and individual factors on mental health literacy.

Abstract TMP49: Radiomics Model Predicts Histological Thrombus Composition on Admission CT Imaging

Background: Red blood cells (RBC)-rich thrombus are more easily retrieved via endovascular procedures while platelet-rich thrombus are more resistant to recanalization. Our aim was to generate a radiomics model able to identify both RBC and platelet-rich thrombus at CT admission in patients undergoing mechanical thrombectomy. Methods: We included consecutive patients that received mechanical thrombectomy due to a large vessel occlusion in which thrombi was obtained. Thrombi obtained during the procedure were hematoxiline-eosine processed and proportions of RBC were determined. Relative proportion of the platelets in the thrombi was quantified by using a immunohistochemical staining recognizing CD61. We considered RBC-rich thrombi those with a content of RBC&gt;30% and platelet-rich thrombi those with a content of CD61&gt;70%. Thrombi were segmented manually on co-registered non-contrast CT (NCCT) and CT angiography (CTA) at admission (&lt;1mm thickness). 804 quantitative radiological features were extracted from the NCCT and CTA images. The most relevant features were selected with a genetic algorithm. Xgboost models were trained and one-versus-all cross-validated on the selected features to classify RBC and platelet-rich thrombi. Results: From 97 patients with histologically and radiologically available thrombi, 43% were female and mean age was 71(±12) years. The area under the curve for detecting RBC&gt;30% was 0.938 (sensitivity 86%, specificity 86%, PPV 84%). Regarding platelet-rich thrombus, the area under the curve for detecting CD61&gt;70% was 0.89 (sensitivity 83%, specificity 84%, PPV 74%). Feature importance by imaging type for RBC-rich and platelet rich thrombus was 70.33% and 69.8% for NCCT and 29.67% and 30% for CTA, respectively. The most important feature types for RBC-rich thrombus were histograms (51%) and first order (27%) while the most important features for platelet rich-thrombus were histograms (94%) followed by texture (5.94%). No information about shape improved any model. Conclusion: Our radiomics model can reliably identify RBC and platelet-rich thrombi. Fast identification of thrombus histological components on CT at arrival can help to design the preferred therapeutic strategy

Quality of Life in Patients with Phenylketonuria: A Systematic Review.

The impact of phenylketonuria (PKU) on Quality of life (QoL) has been a topic of interest in recent research. This article reviews current researches on the impact of PKU on QoL. The review examines factors that may influence QoL, such as age, metabolic control, and treatment adherence. In this systematic review study, relevant articles were identified using a search strategy built with the keywords phenylketonuria, PKU, or hyperphenylalaninemia (or their synonyms) and QoL in Web of Science, Scopus, and PubMed databases. After identifying the articles, duplicates, reviews, scientific abstracts, articles published in languages other than English, and non relevant studies were excluded. The search strategy identified 951 records from databases, and after excluding duplicates, irrelevant studies, and those published in non English languages, 26 records were left that contained data on 1816 patients with PKU/hyperphenylalaninemia. The studies included both children/adolescents and adults. Overall, the studies found that the QoL of PKU patients was comparable to normative data, but some aspects such as emotional health and school functioning were lower. Metabolic control was found to significantly correlate with QoL. Younger patients and men had better QoL in several studies, while late treated patients and those with lower education had worse outcomes. It is concluded that QOL in patients with PKU is similar to the general population. However, given the chronic nature of the condition, it is important to pay special attention to their QoL. Poor QOL is associated with female gender, lower education, older age, and poor metabolic control.

Trauma exposure and associated sleep problems in Black adolescent and young adult students attending an alternative high school.

Given the dearth of literature examining the link between trauma exposure and sleep among youth and young adults of color, the current study examined the association between individual types of trauma exposure, accumulation within subtypes of trauma exposure, and overall cumulative trauma exposure with sleep problems in a sample of Black students attending an alternative high school. Participants were recruited from an alternative high school in a large, southeastern city in the United States, of which all students qualify for free/reduced lunch. The sample included 101 students (53% female) ages 16-24 (M = 17.86 years, SD = 1.36) who identified as Black. Participants reported a high rate of trauma exposure (M = 6.03 unique traumatic experiences, SD = 2.63). Linear regression models showed that exposure to more overall cumulative trauma and to interpersonal loss was significantly associated with higher levels of insomnia symptoms. Threats to health were significantly related to daytime sleepiness. Other threats to safety were associated with restless legs syndrome symptoms. Adolescence and young adulthood are a time of complex sleep-related issues. Black youth and young adults have elevated risk of trauma exposure and sleep problems; therefore, targeted assessment and intervention are warranted. Clinicians and researchers addressing sleep in youth and young adults, and those working within alternative school settings, should also consider a trauma-informed lens to optimize outcomes. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Deceleration Profiles Between the Penultimate and Final Steps of Planned and Reactive Side-Step Cutting.

Noncontact anterior cruciate ligament injury often occurs during rapid deceleration and change-of-direction maneuvers. These activities require an athlete to generate braking forces to slow down the center of mass and change direction in a dynamic environment. During preplanned cutting, athletes can use the penultimate step for braking before changing direction, resulting in less braking demand during the final step. During reactive cutting, athletes use different preparatory movement strategies during the penultimate step when planning time is limited. However, possible differences in the deceleration profile between the penultimate and final steps of preplanned and reactive side-step cuts remain unknown. To comprehensively evaluate deceleration during the penultimate and final steps of preplanned and reactive cutting. Cross-sectional study. Laboratory. Thirty-six women (age = 20.9 ± 1.7 years, height = 1.66 ± 0.07 m, mass = 62.4 ± 8.7 kg). Participants completed 90° side-step cutting maneuvers under preplanned and reactive conditions. Approach velocity, velocity at initial contact, and cutting angle were compared between conditions. Stance time, deceleration time, and biomechanical indicators of deceleration were assessed during the penultimate and final steps of preplanned and reactive 90° cuts. Separate repeated-measures analysis-of-variance models were used to assess the influence of step, condition, and their interaction on the biomechanical indicators of deceleration. Approach velocity (P = .69) and velocity at initial contact of the penultimate step (P = .33) did not differ between conditions. During reactive cutting, participants achieved a smaller cutting angle (P < .001). We identified a significant step-by-condition interaction for all biomechanical indicators of deceleration (P values < .05). A lack of planning time resulted in less penultimate step braking and greater final step braking during reactive cutting. As a result, participants exhibited a decreased cutting angle and longer stance time during the final step of reactive cutting. Improving an athlete's ability to respond to an external stimulus may facilitate a more effective penultimate step braking strategy that decreases the braking demand during the final step of reactive cutting.

Analysis of factors related to the development of ocular biometric parameters in Chinese children aged 6–10 years: a cross-sectional study

ObjectivesEmmetropia depends on the precise coordination of ocular biometry, including axial length (AL), corneal curvature, lens thickness and anterior chamber depth (ACD). Disruption of this coordination leads to refractive errors...

The use of natural minerals compound fertilizers for the production of sweet corn

The natural mineral of polyhalite, which consists of potassium, sulphur, magnesium, and calcium, has the potential to be to supply various essential nutrients to complete the plant’s nutrient requirement, such as sweet corn. The aim of this study to determine the effect of multiple doses of K-Ca-Mg-S fertilizer on sweet corn plants. This research was conducted at Medan City, North Sumatra, with a height of ± 25 meters above sea level from March to May 2023. This study used a Non-Factorial Randomized Block Design with 5 replications and 5 treatments consisting of the dose of fertilizer, namely 0; 2.14, 4.28, 6.42, and 8.57 g/plant. The results of the study showed that several doses of K-Ca-Mg-S compound fertilizer were able to accelerate the male flowering age, which was 53.8 days after planting (dap), the female flowering age was 57.4 dap, and was able to increase the weight of husked cobs, namely 157.758 kg, and seed sugar content of 15.6 % Brix, but did not increase the harvest index.

Abstract WP192: Impact of Second Line Thrombectomy Technique Following Failed First Pass Thrombectomy for Anterior Circulation Stroke: To Switch or Not to Switch?

Introduction: Despite comparable outcomes for different frontline techniques in mechanical thrombectomy (MT) for acute ischemic stroke (AIS), there are sparse data regarding if and when to switch techniques if the first pass is unsuccessful. We aimed to investigate the impact of converting from one MT technique to another on the second MT attempt for AIS among patients with large vessel occlusion (LVO). Methods: This was a retrospective observational study using data from the large multicenter international Stroke Thrombectomy and Aneurysm Registry (STAR). Data from 29 stroke centers for 10,229 AIS patients treated with MT for LVO between January 2010 and December 2022 was investigated. The primary outcome measure was successful recanalization defined as modified Thrombolysis in Cerebral Ischemia (mTICI) score of 2b or higher. 90-day modified Rankin score (mRS) 0-2, mortality and symptomatic hemorrhage were used as secondary outcomes. Clinical and technical outcomes after the second MT attempt were compared between those with or without technique conversion. Results: Among 10,229 AIS patients, 1,797 AIS patients with LVO failed first pass recanalization and were included in this retrospective analysis. 927 patients were female (52%) and median (interquartile range) age was 72 (61-81) years. Converting to alternative techniques following a first failed attempt was more likely to be associated with successful recanalization at the second attempt (adjusted odds ratio 2.30, 95% CI: 1.37-3.86, P = 0.002) and 90-day good clinical outcome (adjusted odds ratio 2.10, 95% CI: 1.15-3.85, P = 0.02) after multivariate adjustment. Conclusions: This study demonstrates better clinical and technical outcomes with conversion of MT technique for the second attempt in AIS patients with LVO who failed first pass recanalization.

A Case series of Tinea corporis caused by &lt;i&gt;Microsporum audouinii&lt;/i&gt;: sharing is not always caring

Tinea corporis caused by Microsporum audouinii is reported herein from two Indian adult females of age 27 and 33 years who are sisters. The fungus was reported from both based on microscopic and culture findings. Both cases had a satisfactory response to the ketoconazole and tolnaftate combination. Infections by this dermatophyte barring tinea capitis are few and far between and hardly reported. Hence, this is an exquisite case series to be reported in the literature. This report also highlights measures to be taken to prevent the transmission of fungal mold through fomites.

Association of platinum-based chemotherapy with live birth and infertility in female survivors of adolescent and young adult cancer

ObjectiveLack of treatment-specific fertility risks for female survivors of adolescent and young adult cancer limits counseling on fertility preservation decisions. The objective was to estimate the effect of platinum-based chemotherapy on live birth and infertility after cancer. DesignRetrospective cohort study using U.S. administrative data ParticipantsWe identified incident breast, colorectal and ovarian cancer cases in females ages 15-39 who received platinum-based chemotherapy or no chemotherapy and matched them to females without cancer. ExposurePlatinum-based chemotherapy OutcomesWe estimated the effect of chemotherapy on incidence of live birth and infertility after cancer, overall and after accounting for competing events (recurrence, death, sterilizing surgeries). ResultsThere were 1,287 survivors in the chemotherapy group, 3,192 survivors in the no chemotherapy group, and 34,147 women without cancer, mean age 33. Accounting for competing events, overall five-year live birth incidence was lower in the chemotherapy group (3.9%) versus the no chemotherapy (6.4%) group. Adjusted relative risks versus no chemotherapy and no cancer were 0.61 (95%CI 0.42-0.82) and 0.70 (95%CI 0.51-0.93), respectively. Overall five-year infertility incidence was similar in the chemotherapy group (21.8%) compared to no chemotherapy group (20.7%). The adjusted relative risks versus no chemotherapy and no cancer were 1.05 (95%CI 0.97-1.15) and 1.42 (95%CI 1.31-1.53), respectively. Conclusions and relevanceCancer survivors treated with platinum-based chemotherapy experienced modestly increased adverse fertility outcomes. Estimated effects of platinum-based chemotherapy were affected by competing events, suggesting the importance of this analytic approach for interpretations that ultimately inform clinical fertility preservation decisions.

Prevalence of autoimmune diseases in an admixed population of patients with type 1 diabetes: a multicenter study in Brazil

Background/objectivesThe primary aim of this study was to evaluate the prevalence of autoimmune diseases (AIDs) and its associated factors in an admixed Brazilian population of patients with type 1 diabetes (T1D). The secondary one was to determine the relationship between AIDs and the occurrence of diabetes-related chronic complications (DRCC).MethodsThis cross-sectional, nationwide survey was conducted in 13 public clinics in 11 Brazilian cities. Overall, 1,760 patients were included; 967 females (55.9%), 932 (54%) Caucasians, aged 29.9 ± 11.9 years, age at diagnosis 14.8 ± 8.9 years, diabetes duration 15.5 ± 9.3 years and 12.2 ± 3.8 years of school attendance. AIDs were retrieved from medical records or self-report and stratified as follows: absence of AIDs, only autoimmune thyroid disease (AITD), and other AIDs including the combination with AITD (hyper or hypothyroidism).ResultsThe prevalence of AIDs was 19.5% being AITDs (16.1%), the most frequently found. A higher prevalence of hypertension, dyslipidemia and overweight or obesity was found in patients who had exclusively AITDs. A higher prevalence of diabetic retinopathy (DR) was observed in patients with AITDs and patients with other AIDs in combination with AITDs. Chronic kidney disease (CKD) was more prevalent in patients with only AITDs. Lower levels of HbA1C, were observed in patients with isolated AITDs or with other AIDs, regardless of the presence of AITD. Hierarchical multivariate analysis, showed that AIDs were associated with female gender, older age, and longer diabetes duration, self-reported color-race (White and Brown), geographic region (Brazilian North/Northeast region) and higher anti-TPO levels (≥ 35 UI/ml).ConclusionsIn conclusion, Brazilian patients with T1D, belonging to a highly ethnically admixed population, had an important prevalence of AIDs, mostly AITDs, that was associated with female gender, self-reported color-race, older age and longer diabetes duration. Moreover, these patients also had a higher prevalence of DRCC. Even though we highlight the importance of investigating the presence of AIDs at diagnosis and at regular intervals, it is unclear whether screening and early detection of additional AIDs may improve the clinical outcomes in individuals with T1D. Future prospective studies are necessary to establish the interplay between T1D, AIDs and DRCC.

Pouch of sorrow: Undiagnosed isthmocele – presenting as secondary infertility of 11 years in a middle age female

In the past decade, the rate of cesarean sections has doubled leading to the more frequent occurrence of cesarean-related complications. Cesarean scar site diverticulum or isthmocele is one such complication that is defined as an outpouching of the anterior uterine wall at the cesarean scar site in continuity with the endometrial cavity, creating a wedge-shaped defect of variable depth. We report a case of a middle-aged female with secondary infertility for the last 11 years with one live issue 12 years back through a cesarean section at term. She had undergone an array of investigations for infertility for the past 5 years. She was diagnosed as having an isthmocele and managed with surgical repair of the defect at our hospital with subsequent assisted conception 8-month post-surgical repair. The importance of diagnosing a cesarean scar diverticulum cannot be understated not only because it is a surgically treatable cause of infertility but also because if such patients conceive, they may land in uterine rupture, placenta accreta, or scar ectopic pregnancy which can complicate into life-threatening situations.

Relationship of the bone phenotype of the Klotho mutant mouse model of accelerated aging to changes in skeletal architecture that occur with chronological aging.

Due to the relatively long life span of rodent models, in order to expediate the identification of novel therapeutics of age related diseases, mouse models of accelerated aging have been developed. In this study we examined skeletal changes in the male and female Klotho mutant (kl/kl) mice and in male and female chronically aged mice to determine whether the accelerated aging bone phenotype of the kl/kl mouse reflects changes in skeletal architecture that occur with chronological aging. 2, 6 and 20-23 month old C57BL/6 mice were obtained from the National Institute of Aging aged rodent colony and wildtype and kl/kl mice were generated as previously described by M. Kuro-o. Microcomputed tomography analysis was performed ex vivo to examine trabecular and cortical parameters from the proximal metaphyseal and mid-diaphyseal areas, respectively. Serum calcium and phosphate were analyzed using a colorimetric assay. The expression of duodenal Trpv6, which codes for TRPV6, a vitamin D regulated epithelial calcium channel whose expression reflects intestinal calcium absorptive efficiency, was analyzed by quantitative real-time PCR. Trabecular bone volume (BV/TV) and trabecular number decreased continuously with age in males and females. In contrast to aging mice, an increase in trabecular bone volume and trabecular number was observed in both male and female kl/kl mice. Cortical thickness decreased with advancing age and also decreased in male and female kl/kl mice. Serum calcium and phosphate levels were significantly increased in kl/kl mice but did not change with age. Aging resulted in a decline in Trpv6 expression. In the kl/kl mice duodenal Trpv6 was significantly increased. Our findings reflect differences in bone architecture as well as differences in calcium and phosphate homeostasis and expression of Trpv6 between the kl/kl mutant mouse model of accelerated aging and chronological aging. Although the Klotho deficient mouse has provided a new understanding of the regulation of mineral homeostasis and bone metabolism, our findings suggest that changes in bone architecture in the kl/kl mouse reflect in part systemic disturbances that differ from pathophysiological changes that occur with age including dysregulation of calcium homeostasis that contributes to age related bone loss.

The Estimation of Cuscus Population and Distribution in Teluk Wondama District, West Papua Province, Indonesia

Cuscus is a marsupial mammal endemic to the eastern part of Indonesia. Populations and their habitats are under pressure due to economic development, population growth and utilization of forest resources. So far, studies on cuscus ecology have mainly focused on protected habitats or conservation areas, this study aims to identify and analyze populations and predict the distribution of cuscus in non-protectes area which is PT Wijaya Sentosa. This research conduct in November 2020 until January 2021 in several land cover categories. The methods used in this study include literature and secondary data studies, field observations using strip-transect and camera trap techniques, interviews using purposive sampling techniques. This study found nine individuals of cuscus from direct observation with strip-transects and two individuals of cuscus from camera trapping. The ground cuscus population (P. gymnotis) had the highest abundance and density, while the common spotted cuscus (S. maculatus) population owned the lowest values. The population structure dominates by male sex and adult age class, and no female sex and young age class were found. Cuscus was more commonly found solitary in the line of observation and not found in every line. The shape of the distribution pattern of the cuscus population at PT Wijaya Sentosa is cluster/aggregated, determined from the value of the dispersion index and the ratio of variance to mean. The most common ground cuscus was found in the 2018 and the 2013 logging area (LOA).

Preimplantation genetic testing in couples with balanced chromosome rearrangement: a four-year period real world retrospective cohort study

BackgroundCouples with balanced chromosome rearrangement (BCR) are at high risk of recurrent miscarriages or birth defects due to chromosomally abnormal embryos. This study aimed to provide real-world evidence of the euploidy rate of blastocysts from couples with BCR using preimplantation genetic testing (PGT) and to guide pretesting genetic counselling.MethodsA continuous four-year PGT data from couples with BCR were retrospectively analyzed. Biopsied trophectoderm cells were amplified using whole genome amplification, and next-generation sequencing was performed to detect the chromosomal numerical and segmental aberrations. Clinical data and molecular genetic testing results were analyzed and compared among the subgroups.ResultsA total of 1571 PGT cycles with 5942 blastocysts were performed chromosomal numerical and segmental aberrations detection during the four years. Of them, 1034 PGT cycles with 4129 blastocysts for BCR couples were included; 68.96% (713/1034) PGT cycles had transferable euploid embryos. The total euploidy rate of blastocysts in couples carrying the BCR was 35.29% (1457/4129). Couples with complex BCR had euploid blastocyst rates similar to those of couples with non-complex BCR (46.15% vs. 35.18%, P > 0.05). Chromosome inversion had the highest chance of obtaining a euploid blastocyst (57.27%), followed by Robertsonian translocation (RobT) (46.06%), and the lowest in reciprocal translocation (RecT) (30.11%) (P < 0.05). Couples with males carrying RobT had higher rates of euploid embryo both in each PGT cycles and total blastocysts than female RobT carriers did, despite the female age in male RobT is significant older than those with female RobT (P < 0.05). The proportions of non-carrier embryos were 52.78% (95/180) and 47.06% (40/85) in euploid blastocysts from couples with RecT and RobT, respectively (P > 0.05). RecT had the highest proportion of blastocysts with translocated chromosome-associated abnormalities (74.23%, 1527/2057), followed by RobT (54.60%, 273/500) and inversion (30.85%, 29/94) (P < 0.05).ConclusionsIn couples carrying BCR, the total euploidy rate of blastocysts was 35.29%, with the highest in inversion, followed by RobT and RecT. Even in couples carrying complex BCR, the probability of having a transferable blastocyst was 46.15%. Among the euploid blastocysts, the non-carrier ratios in RecT and RobT were 52.78% and 47.06%, respectively. RecT had the highest proportion of blastocysts with translocated chromosome-associated abnormalities.