Feedstock For Biodiesel Production Research Articles

Investigation on the Fouling and Heat Transfer Characteristics of Novel EGR Cooler (Semi-spiral) for Diesel Engine Fueled with Biodiesel

In this study, heat transfer characteristic and fouling of Novel EGR cooler (Semi-spiral) system were investigated. In this study, waste vegetable oil (Waste Cooking Oil) was used as feedstock for biodiesel production. The experiment was conducted in different loads (25%, 50% and 75%) and different speeds (2100rpm and 2400rpm). Four fuel blends were prepared and used (B0, B5, B10, and B15). The new EGR cooler has 6 tubes in length of 19 cm and overall heat transfer coefficient of 35.63 W/m^2 K which is acceptable with compared to overall heat transfer coefficient of shell and tube heat exchanger. Also, the total resistance of fouling (Rft) derived was 0.002712 m^2 K/W. The maximum heat transfer efficiency is 94% and the minimum heat transfer efficiency is 68%. Also, the results showed that when B0 is used, the deposition was 1.37 gr that amounts to 0.23gr/hr. When B15 is used, the deposition was I.21gr that amounts to 0.20gr/hr.

Recent Updates on Biodiesel Production Techniques: A Review

The present review presents the most commonplace vegetable-based feedstock for biodiesel production. It focuses on biodiesel production with an emphasis on the most recent studies and innovations. Transesterification of plant oil or animal fat is the most common process for the production of biodiesel. Several techniques are utilized for this transesterification reaction, such as batch processes, ultrasonic, microwave and other methods. Many conditions are usually studied, including temperature, pressure, solvent and catalysis. Furthermore, we aim to provide an investigation of the different processes and technologies applicable to the production of second-generation biodiesel, with special attention paid to the development of innovative catalysts as well as new reactor concepts.

Physicochemical Characterization of Jatropha podagrica Seed Oil for Potential Biodiesel Production and other Industrial Applications in Thailand

Jatropha is considered as one of the most promising potential oil sources for biodiesel production and other industrial applications. However, research on the potential of Jatropha seed oil is mainly focused on Jatropha curcas, with other species receiving little attention. The physicochemical properties of J. podagrica seed oil was studied to determine its potential as feedstock for biodiesel production and other industrial applications in Thailand. The seed oil was extracted with n-hexane from milled kernels using the soxhlet extractor and subsequently characterised for free fatty acids, iodine value, viscosity, saponification value, density, and acid value. The fatty acid profile of the seed oil was also analysed using gas chromatography (GC). Analysis of the physical properties of the J. podagrica seed kernel showed lower average physical characteristics when compared to those of J. curcas seed kernel. J. podagrica seeds had high oil content comparable to J. curcas oil content. The main fatty acid components of the seed oil were oleic acid (15%) and linoleic acid (70%). Generally, the results of the physicochemical analysis indicated that J. podagrica seed oil would be very useful for the production of soap and shampoo in Thailand. To produce biodiesel from the seed oil, a two-step acid-catalysed transesterification process would be appropriate.

The Potential of Single-Cell Oils Derived From Filamentous Fungi as Alternative Feedstock Sources for Biodiesel Production.

Microbial lipids, also known as single-cell oils (SCOs), are highly attractive feedstocks for biodiesel production due to their fast production rates, minimal labor requirements, independence from seasonal and climatic changes, and ease of scale-up for industrial processing. Among the SCO producers, the less explored filamentous fungi (molds) exhibit desirable features such as a repertoire of hydrolyzing enzymes and a unique pellet morphology that facilitates downstream harvesting. Although several oleaginous filamentous fungi have been identified and explored for SCO production, high production costs and technical difficulties still make the process less attractive compared to conventional lipid sources for biodiesel production. This review aims to highlight the ability of filamentous fungi to hydrolyze various organic wastes for SCO production and explore current strategies to enhance the efficiency and cost-effectiveness of the SCO production and recovery process. The review also highlights the mechanisms and components governing lipogenic pathways, which can inform the rational designs of processing conditions and metabolic engineering efforts for increasing the quality and accumulation of lipids in filamentous fungi. Furthermore, we describe other process integration strategies such as the co-production with hydrogen using advanced fermentation processes as a step toward a biorefinery process. These innovative approaches allow for integrating upstream and downstream processing units, thus resulting in an efficient and cost-effective method of simultaneous SCO production and utilization for biodiesel production.

Molecular Mechanism of Lipid Accumulation and Metabolism of Oleaginous Chlorococcum sphacosum GD from Soil under Salt Stress.

The oleaginous microalgae species Chlorococcum sphacosum GD is a promising feedstock for biodiesel production from soil. However, its metabolic mechanism of lipid production remains unclear. In this study, the lipid accumulation and metabolism mechanisms of Chlorococcum sphacosum GD were analyzed under salt stress based on transcriptome sequencing. The biomass and lipid content of the alga strain were determined under different NaCl concentrations, and total RNA from fresh cells were isolated and sequenced by HiSeq 2000 high throughput sequencing technology. As the salt concentration increased in culture medium, the algal lipid content increased but the biomass decreased. Following transcriptome sequencing by assembly and splicing, 24,128 unigenes were annotated, with read lengths mostly distributed in the 200–300 bp interval. Statistically significant differentially expressed unigenes were observed in different experimental groups, with 2051 up-regulated genes and 1835 down-regulated genes. The lipid metabolism pathway analysis showed that, under salt stress, gene-related fatty acid biosynthesis (ACCase, KASII, KAR, HAD, FATA) was significantly up-regulated, but some gene-related fatty acid degradation was significantly down-regulated. The comprehensive results showed that salt concentration can affect the lipid accumulation and metabolism of C. sphacosum GD, and the lipid accumulation is closely related to the fatty acid synthesis pathway.

Experimental Investigation and Optimization of Non-Catalytic In-Situ Biodiesel Production from Rice Bran Using with RSM Historical Data Design

Biodiesel has become one of the essential fuels in the present and future, and it can be produced from vegetable oil and animal fat. However, current feedstocks to produce biodiesel slow down the growth of biodiesel implementation due to the high cost of feedstock. As a result, rice bran oil (RBO) is claimed to be a potential feedstock for biodiesel production. A non-catalytic in-situ biodiesel production from low cost feedstock (RBO) using subcritical ethanol-water mixture probably decrease the production cost further and environmentally benign. Therefore, in this work the influence of four independent variables, adding co-solvent/without co-solvent, ethanol concentration, temperature, and time of reactions on the yield of biodiesel was examined tentatively. The independent variables limitations were (a) co-solvent of n-hexane, ethyl acetate and chloroform, (b) ethanol concentration of 20 - 80 %, v/v, (c) temperature of reaction 120 - 200 oC, and (d) time of reaction 1 - 4 h employed to direct the trials. In this examination historical data design was utilized and a quadratic polynomial model was built up and after that at last optimized by using response surface methodology (RSM). It was found that the yield of biodiesel achieved an optimum value of 79.79 %, v/v using ethyl acetate as co-solvent with ethanol concentration of 78.83 % (v/v), under constant operation conditions of P = 8 Mpa, T = 160 oC, t = 2 h, N = 400 rpm, ratio of rice bran (RB): solvent: co-solvent= 10 (g): 80 mL: 20 mL. In the other hand (rice bran 2), it was found that the yield of biodiesel achieved an optimum value of 76.98 % for 3.2 h of reaction time and temperature of reaction 200 oC, under constant operation conditions of P = 8 Mpa, co-solvent = ethyl acetate, ethanol concentration = 80 %, v/v, N = 400 rpm, ratio of RB: solvent: co-solvent= 10 (g): 80 mL: 20 mL. The examination has likewise uncovered that authentic plan information with RSM is a well-organized statistical technique for forecasting the optimum operating conditions of a non-catalytic in-situ biodiesel production from rice bran using subcritical ethanol-water mixture assumes a crucial role of ethanol concentration, types of co-solvent, temperature and time of reactions.

Candlenut oil: review on oil properties and future liquid biofuel prospects

The rapid depletion of diesel fuel, increasing energy demand, and environmental pollution concerns are increasing worldwide interest in the production of liquid biofuels. Biofuel (bio-aviation and biodiesel) is a potential and plausible alternative to diesel fuel to substantially mitigate the environmental impact of future energy demand. Non-edible crop oil is viewed as a potential feedstock for liquid biofuel production owing to the massive demand for edible oil as a food source. However, the major limitation of utilizing non-edible crop oil for biodiesel production is the cost due to the high price of feedstocks and the limited supply of large-scale biodiesel production. Candlenut trees can grow in harsh and arid climates due to low moisture requirements. Therefore, candlenut can be cultivated in the most unused lands, particularly in developing countries along coasts and riverbanks and in deserts and other wastelands unsuitable for edible crops. Additionally, candlenut seed contains a high amount of oil (30%-60%). Thus, candlenut oil is a promising source for commercial biodiesel production. The present study was conducted to review the possibilities and challenges of utilizing candlenut as a potential feedstock for biodiesel production. Additionally, several important aspects related to candlenut oil processing, such as extraction technology, physicochemical properties, biodiesel production technologies, and advantages and limitations of candlenut biodiesel production are discussed.

Correction to: Biodiesel production from microalgae Dunaliella tertiolecta: a study on economic feasibility on large-scale cultivation systems

Microalgae have attracted significant interest worldwide as a prospective biofuel feedstock. Currently, biodiesel potential of microalgae has been confined at lab scale; however, economic feasibility is a limiting factor for commercialization. Thus, development of large-scale production system is an essential element to assess economic feasibility as well as policy support needed to implement large-scale production. To answer this, Dunaliella tertiolecta was selected for biodiesel feedstock production among other isolates, having 1.244 g/L dry biomass yield and 37%w/w lipid content. Operational cost of biodiesel production was 3.19 USD/L for lab scale which can be reduced to 0.77 USD/L at large-scale production by integrated and efficient resource utilization, namely, natural seawater (NSW), as growth media and unproductive coastland for production site. Capital investment of USD 82019.89 was calculated for biodiesel production facility with 16.7 KL annual production for one-hectare unproductive land. Economic evaluation of 10 years of production resulted into net present value (NPV) of USD 750.91 and 5.18% internal rate of return (IRR). Capital recovery can be achieved in 7.76 years as per normal payback analysis. Additionally, NPV can be increased to USD 30355 by upscaling production to five times with 50% more biomass productivity that would result into 14.23% IRR. Efficient resource integration, multiple products and co-products with market linkages, and appropriate policy facilitation are key factors for commercial viability of microalgae biodiesel production.

Evaluation of Thirty Microalgal Isolates as Biodiesel Feedstocks Based on Lipid Productivity and Triacylglycerol (TAG) Content.

Microalgae are considered feedstock for biodiesel production due to their capability to accumulate triacylglycerols, which have a 99% conversion rate into biodiesel, under certain conditions. This study aims to evaluate thirty native microalgal strains as feedstock for biodiesel production based on their biomass and lipid productivities, and total lipid and triacylglycerol contents under nitrogen-sufficient and nitrogen starvation conditions. In addition, Chlamydomonas reinhardtii cw15 mutant strain was utilized as a reference strain for triacylglycerol accumulation. Among the eight potent strains, Chlorella vulgaris KP2 was considered as a most promising strain with the highest triacylglycerol content, highest total lipid content (28.56% of dry cell weight), and the highest lipid productivity (4.56mg/L/day) under nitrogen starvation. Under nitrogen starvation, the major fatty acids in the triacylglycerol ofChlorella vulgaris KP2 wereC18:1 (37.56%), C16:0 (23.16%), C18:0 (23.07), C18:2 (7.00%), and C18:3 (3.12%), and the percentages of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids represented 49.26, 38.73, and 10.12% of the total fatty acids, respectively. Furthermore, the fatty acid methyl esters of triacylglycerol displayed remarkable biodiesel properties with a lower iodine value (59.00 gI2/100g), higher oxidative stability (14.24h) and higher cetane number (58.73) under nitrogen starvation. This study suggests that nitrogen-starved Chlorella vulgaris KP2 could be used as a feedstock for biodiesel production due to the considerable amounts of triacylglycerol and favorable biodiesel properties.

Enhancement of Tobacco (Nicotiana tabacum L.) Seed Lipid Content for Biodiesel Production by CRISPR-Cas9-Mediated Knockout of NtAn1.

Tobacco (Nicotiana tabacum L.) seed lipid is a promising non-edible feedstock for biodiesel production. In order to meet the increasing demand, achieving high seed lipid content is one of the major goals in tobacco seed production. The TT8 gene and its homologs negatively regulate seed lipid accumulation in Arabidopsis and Brassica species. We speculated that manipulating the homolog genes of TT8 in tobacco could enhance the accumulation of seed lipid. In this present study, we found that the TT8 homolog genes in tobacco, NtAn1a and NtAn1b, were highly expressed in developing seed. Targeted mutagenesis of NtAn1 genes was created by the CRISPR-Cas9-based gene editing technology. Due to the defect of proanthocyanidin (PA) biosynthesis, mutant seeds showed the phenotype of a yellow seed coat. Seed lipid accumulation was enhanced by about 18 and 15% in two targeted mutant lines. Protein content was also significantly increased in mutant seeds. In addition, the seed yield-related traits were not affected by the targeted mutagenesis of NtAn1 genes. Thus, the overall lipid productivity of the NtAn1 knockout mutants was dramatically enhanced. The results in this present paper indicated that tobacco NtAn1 genes regulate both PAs and lipid accumulation in the process of seed development and that targeted mutagenesis of NtAn1 genes could generate a yellow-seeded tobacco variety with high lipid and protein content. Furthermore, the present results revealed that the CRISPR-Cas9 system could be employed in tobacco seed de novo domestication for biodiesel feedstock production.

Open system for the autotrophic cultivation of Scenedesmus obliquus NCIM 5586: multiobjective optimization for the tradeoff between biomass and lipid

Microalgae are used as a third-generation biofuel feedstock to overcome the problems, of first- and second-generation feedstocks, such as food versus fuel competition and longer harvesting time. However, the use of microalgae as a feedstock for biodiesel production is limited by the lipid content in the biomass. The development of strategies to enhance both the biomass and lipid content would lead to low-cost processes for biofuel production. In the present study, NaNO3, K2HPO4, NaCl, and initial medium pH were optimized to enhance both biomass yield and lipid content of freshwater microalgae Scenedesmus obliquus NCIM 5586 in a 5-L open pond system under light and dark period using central composite design. A multiobjective optimization method is employed to find the tradeoff between biomass and lipid accumulation and to predict the optimum composition for the multiple responses. The maximized yield of biomass and lipid was observed when concentrations of NaNO3, K2HPO4, and NaCl were 1.60 μM, 178.38 μM, and 24.80 mM, respectively, with initial medium pH of 7.23. The final yields of biomass and lipid at optimized conditions were recorded to be 1275.8 mg/L/day and 50.66% dcw, respectively. The obtained results indicate the significance of Scenedesmus obliquus cultivation in the open pond for the generation of feedstock for 3G biofuels.

Transesterification via Parametric Modelling and Optimization of Marula (Sclerocarya birrea) Seed Oil Methyl Ester Synthesis.

This study investigates Marula (Sclerocarya birrea) seed oil (SBSO) as a novel feedstock for biodiesel production through the transesterification process catalysed by heterogeneous bio-alkali derived from banana (Musa acuminata) peels. Response surface methodology (RSM) and artificial neural network (ANN) tools were used for the modelling and optimization of the process variables. The reaction process parameters considered were methanol/SBSO molar ratio, catalyst loading levels, reaction time and temperature. Central composite design (CCD) was espoused to generate 30 experimental conditions which were deployed in investigating the individual and synergetic effect of the process input variables on Sclerocarya birrea oil methyl ester (SBOME) yield. Appropriate statistical indices were adopted to investigate the predictive aptitude of the two models. Analysis shows that ANN model obtained for the transesterification process has a higher coefficient of determination (R2) of 0.9846 and lower absolute average deviation (AAD) of 0.07% compared to RSM model with R2 of 0.9482 and AAD of 0.12%. The process modelling outcome also confirmed ANN performance to be more precise than RSM. At methanol/SBSO ratio of 6:1, catalyst loading level of 2 wt%, process reaction time of 50 min and temperature of 55°C, the experimental maximum SBOME yield was observed to be 96.45 wt % following the ANN predicted yield of 96.45 wt % and RSM predicted yield of 96.65 wt % respectively. The analysed fuel properties of SBOME was found satisfactory within the biodiesel stipulated standard limit(s). The study establishes that SBSO is a good source for biodiesel production and its biodiesel methyl ester is a potential substitute for petroleum diesel and a bioenergy fuel.

Emission and performance behavior of hemp seed oil biodiesel/diesel blends in DI diesel engine

Hemp seed oil is chosen as a feedstock for biodiesel production, methanol and KOH were used in the biodiesel production. The properties like chemical and physical of hemp oil biodiesel are measured to investigate the engine emission and performance characteristics of diesel engine. From the results, B20 blend performance is best among other fuels tested. Besides, the Brake Specific Fuel consumption (BSFC) at full load (0.3 kg/kW h) for B20 is slightly lesser than diesel fuel (0.32 kg/kW h). This indicates that the lower diesel/hemp seed oil biodiesel blends will increase the performance as well as decrease the fuel consumption. Considering, the emissions from exhaust exhibited that Carbon Monoxide (CO), Hydrocarbon (HC) and Nitrox oxide (NOx) were lowered for all hemp seed oil biodiesel/diesel blends. However, smoke emission increased slightly than diesel. In general, the results concluded that hemp oil biodiesel is a best alternative as well as good substitute fuel to diesel.

Algae as a feedstock for biodiesel production in Indian perspective

Fast-growing population and transportation sectors have created a need to adopt a socio-economical way to produce and utilize the novel alternative fuel as an energy source all across the world. In the search for new alternative fuel, Biodiesel was found to be a reliable, and a clean fuel, having less tailpipe emission which can improve engine performance and lower the tailpipe emission while reducing the dependency on diesel. In this review, the third-generation feedstock, i.e., Algae has been selected and studied for biodiesel production. Algae has a high oil yield, carbon neutral, and booming nature. The main objective of this research work is the study of biodiesel production and its application on Diesel engine with and without nanoparticles additives. To understand critical importance of production, performance and emission aspects, a comparative study was performed. Analysis of algae as a biofuel from various research work showed that optimum concentration of additives in biodiesel blend can improve the engine performance, such as, BSFC and BTE, and further reduce the tailpipe emission especially NOX. This study reveals performance analysis of Algae as a biofuel and various advantages of using nano additives in biodiesel blends. We believe that this study will help to address the crucial research gap on biodiesel blends and nanoparticles as fuel in compression ignition engine.

Esterification of a waste cooking oil using metal-hybrid catalysts for chemoenzymatic reactions

Waste cooking oil (WCO) serve as a cheap feedstocks for biodiesel production. However, the high acidity of this oil disqualifies it from using alkaline transesterification as the synthesis reaction for biodiesel production. Thus, the integration of enzymatic and chemical catalysts to form a hybrid catalyst for reducing oil acidity is a new option in the biodiesel industry. In the present study, five types of metal oxides and Candida rugosa lipase (CRL) were sequentially co-immobilized on the Alumina (Al2O3) for esterification of free fatty acid (FFA) to fatty acid methyl esters (FAMEs).The lowest FFA content achieved was 1.10% using CRL-CaO-Al2O3 for 24 h esterification reaction, correspond to a conversion rates of 66.94%. The FAMEs conversion of the treated WCO indicates that the hybrid catalyst also can perform transesterification of triglycerides in the oil. Among all the hybrid catalysts, CRL-MgO-Al2O3 shows the highest FAME conversion of 69.10%. The best immobilization time was recorded at 7 h which successfully reduced the FFA content until 1.39%. The application of heterogeneous enzyme-metal hybrid catalysts promise a better insight in the biodiesel industry in terms of its simplicity and catalyst recovery.

Kinetics and Transesterification of the Oil Obtained from <i>Cussonia bateri</i> (Jansa Seed) as a Step in Biodiesel Production Using Natural Heterogeneous Catalyst

The ongoing search for a more sustainable, renewable, and affordable fuel source has necessitated the quest and search for a better diesel. Biodiesel, an environmentally friendly diesel, has been able to solve many of the issues that have arisen as a result of the use of fossil fuels. They are mostly synthesized by transesterification of a FFA with an alcohol employing an appropriate catalyst. This study examines the use of jansa seed oil as a low-cost feedstock for biodiesel production. Transesterification of free fatty acids (FFA) with methanol and ethanol catalyzed by snail shell was used to process the biodiesel. In the biodiesel production, the alcohol to oil molar ratio was 12:1, the catalyst amount was 0.75 g, and the reaction temperature was 65°C. The reversible second-order reaction rate was used to characterize the kinetics of FFA transesterification. Kinetic modeling of the biodiesel production process was also carried out in order to determine the sequence of the reaction and estimate the reaction rate constant. The activation energy of the ethyl ester was higher than that of the methyl ester, implying that the ethyl ester would require more energy (slower reaction rate) to activate a molecule for chemical transformation. The reusability of the catalyst for continuous transesterfication runs was investigated under the same operating conditions, and the conversion of the catalyst declined from 99.6 percent to 86.4 percent after the fifth regeneration cycle. Jansa seed oil has the potential to be a valuable raw source for generating fatty oil for the use as an alternate feedstock in the production of biodiesel.

Microalgae biodiesel and its various diesel blends as promising alternative fuel for diesel engine

Abstract The current scenario of petroleum diesel and its unlimited utilisation by society was lead to investigate the substitute for diesel. In this volatile environment India is mostly depend upon the highly sensitive and unstable countries for its petroleum need. This will directly result on economy as well as growth rate of country. An effective alternative fuel derived from vegetable and non-vegetable feedstock that can be used as fuel in engines is termed as biodiesel. Microalgae can grow in any environment and requires very less land space in comparison with other feedstock. The feedstock to oil conversion ratio of algae is also pretty good, which distinguished it from others. Because of this reasons, Microalgae have been pointed out as a promising biodiesel production feedstock amongst all available sources of biodiesel. This paper focused on microalgae potential, methods and material for biodiesel formation and its blending as well as physiochemical properties obtained from pure microalgae and its blends was also plotted in tabular form for better understanding.

Study on the ethanol production from hydrolysate derived by ultrasonic pretreated defatted biomass of chlorella sorokiniana NITTS3

Abstract Microalgae biomass is considered by many as a most promising renewable source for future generation feedstock for simultaneous production of biodiesel and bioethanol due to the accumulation of considerable amounts of lipids and carbohydrates, respectively. In this work, defatted green microalga, Chlorella sorokiniana NITTS3 biomass was ultrasonic pretreated and effectively used as a culture medium for ethanol production through fermentation using Saccharomyces cerevisiae NITTS1. The ultrasonic pretreatment improved ethanol yield by 25.83 g/L than untreated defatted microalgal biomass. Carbohydrate content of hydrolysate of defatted microalgal biomass after ultrasonic pretreatment was analyzed and found that hydrolysate primarily possessed simple sugars, namely glucose and xylose. Maximally 52.10 ± 0.12 g/L (86.70 ± 0.52 mg bioethanol/g DMB) of ethanol was produced at optimum fermentation conditions of 30 ℃, pH 4 and 200 rpm. This study results show that pretreated microalgal biomass could be used as a cheap, sustainable feedstock for enhanced ethanol production.

Synthesis of biodiesel from Annona muricata – Calophyllum inophyllum oil blends using calcined waste wood ash as a heterogeneous base catalyst

Naturally, biodiesel synthesized from highly viscous and high-density vegetable oil is usually unsuitable as fuel in the internal combustion engine. However, mixing/blending of two or more oils as a feedstock for biodiesel production could produce a low viscous fuel suitable for the engine. This study produced a novel heterogeneous base catalyst from waste wood ash (WWA) and applied it to synthesis of biodiesel from Annona muricata and Calophyllum inophyllum oilseed blend. The production route was via a two-step process due to the high free fatty acid of the blended oil. Process optimization of the transesterification step was carried out via response surface methodology (RSM). The strength of the developed catalyst was tested through catalyst regeneration and recyclability. The quality of the biodiesel was compared with biodiesel recommended standard.•Waste wood ash contained a high percentage of calcium carbonate•Blended oil produced oil of low viscosity•Two-step production route was used for biodiesel synthesis•Process optimization via hybrid design produced optimum biodiesel yield.

Optimization of ultrasound-assisted oil extraction from Canarium odontophyllum kernel as a novel biodiesel feedstock

In this novel study, oil was extracted from the kernel of an exotic indigenous species known as Canarium odontophyllum via an ultrasound-assisted process. The extraction process was optimized using response surface methodology (RSM) based on the Box-Behnken experimental design (BBD). The optimal conditions for the investigated parameters were determined as ultrasound amplitude level: 38.30%, ratio of n-hexane to kernel powder: 50:1 in mL/g, extraction time: 45.79 min, resulting in an oil extraction yield of 63.48%. For verification purposes, experiments were conducted using the same optimized values of the investigated parameters which resulted in the average oil yield of 63.27% and this prove the reliability of the regression model. The extracted oil’s fatty acid composition was obtained using a gas chromatograph (GC) equipped with flame-ionization detection (FID). The low acid value of the extracted oil is another interesting finding. This is important because it circumvents pretreatment processes such as degumming and esterification prior to the transesterification process. Biodiesel was produced from the oil via ultrasound-assisted transesterification, with a yield of 95.2%. Physiochemical properties of the C. odontophyllum biodiesel were determined, and it was found that all the tested properties comply with fuel specifications based on ASTM D6751 and EN 14214 standards. Significant savings of 52.3% and 80.9% in energy consumption and extraction time, respectively were achieved via ultrasound-assisted extraction compared with the conventional Soxhlet extraction. This study establishes the foundation and the need to further explore the usage of C. odontophyllum as a potential feedstock for biodiesel production.